UV-B对大麦幼苗叶片部分生理和光合特性的影响
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摘要
采用室内培养法,研究了模拟UV-B辐射对大麦幼苗叶片部分生理指标和光合特性的影响,以期揭示UV-B辐射对大麦幼苗胁迫效应规律。结果表明,随着UV-B辐射处理(2～6 kJ/m2)强度的增强,大麦幼苗叶片细胞膜透性(CMP)、丙二醛(MDA)含量、胞间二氧化碳浓度(Ci)呈上升趋势,而叶绿素含量、净光合速率(Pn)、气孔导度(Gs)及蒸腾速率(Tr)呈下降趋势;各项指标在T3(6 kJ/m2)处理组与对照间达极显著差异(P<0.01),并且辐射处理时间越长对大麦幼苗叶片的各项生理指标和光合特性的影响越大。
To find UV-B radiation how to influence photosynthesis of Hordeum vulgare seedling leaves, the effects of simulated UV-B radiation on physiological and photosynthetic characteristics of Hordeum vulgare seedling leaves were studied by the indoor cultivation experiments. The results showed that with the increase of UV-B radiation intensity(2-6 kJ/m2), cellular membrane penetration(CMP), malondialdehyde(MDA)content, the concentration of carbon dioxide(Ci)in the Hordeum vulgare seedling leaves showed an upward trend, chlorophyll content, net photosynthetic rate(Pn), stomatal conductance(Gs)and transpiration rate(Tr)showed a decreasing trend. Compared with contrast(0 kJ/m2), the indexes in T3(6 kJ/m2)treatment group reached very significant difference(P<0.01). In addition, the results also showed that the longer the radiation time was, the more serious the effects on physiological and photosynthetic characteristics of Hordeum vulgare seedling leaves were.
To find UV-B radiation how to influence photosynthesis of Hordeum vulgare seedling leaves, the effects of simulated UV-B radiation on physiological and photosynthetic characteristics of Hordeum vulgare seedling leaves were studied by the indoor cultivation experiments. The results showed that with the increase of UV-B radiation intensity(2-6 kJ/m2), cellular membrane penetration(CMP), malondialdehyde(MDA)content, the concentration of carbon dioxide(Ci)in the Hordeum vulgare seedling leaves showed an upward trend, chlorophyll content, net photosynthetic rate(Pn), stomatal conductance(Gs)and transpiration rate(Tr)showed a decreasing trend. Compared with contrast(0 kJ/m2), the indexes in T3(6 kJ/m2)treatment group reached very significant difference(P<0.01). In addition, the results also showed that the longer the radiation time was, the more serious the effects on physiological and photosynthetic characteristics of Hordeum vulgare seedling leaves were.
引文
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[22]胡晓雪,杜维俊,杨珍平.大气CO2浓度和气温升高对野生大豆光合作用的影响[J].山西农业科学,2015,43(7):798-801,853.
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[25]董杰,陈新新,杨倩.高光、水分和盐胁迫下小麦光合特性和抗氧化酶系统的比较[J].麦类作物学报,2018,38(3):315-322.
[26]时丽冉,崔兴国,朱云霞.盐胁迫对不同品种小黑麦光合特性和生长的影响[J].衡水学院学报,2009,11(4):61-64.
[27]黄梅玲,江洪,金清,等. UV-B辐射胁迫下不同起源时期的3种木本植物幼苗的生长及光合特性[J].生态学报,2010,30(8):1998-2009.
[28]石盼盼,宋朝鹏,贺帆,等.不同光质对烤烟旺长期生长发育和光合特性的影响[J].山西农业科学,2013,41(1):36-39,56.
[29]马野,原换换.干旱胁迫下外源油菜素内酯对玉米幼苗光合作用和D1蛋白的调控效应[J].华北农学报,2017,32(3):118-124.
[2] RANJBARFORDOEI A,SAMSON R,VAN DAMME P. Photosynthesis performance in sweet almond exposed to supplemental UV-B radiation[J]. Photosynthetica,2011,49(1):107-111.
[3]吴杏春,林文雄. UV-B辐射增强对两种不同抗性水稻叶片光合生理及超显微结构的影响[J].生态学报,2007,27(2):554-564.
[4]刘兵,王程,金剑,等. UV-B辐射增强对大豆等植物生理生态特性的影响[J].大豆科学,2009,28(6):1097-1100.
[5]殷红,郭巍,毛晓艳,等.增强的UV-B辐射对水稻光合作用的影响[J].作物杂志,2009(4):41-45.
[6]张莉娜,安黎哲,冯虎元.增强UV-B辐射和干旱对春小麦光合作用及其生长的影响[J].西北植物学报,2010,30(5):981-986.
[7] BREZNIK B GERM M,GABERSCIK A. Combined effects of elevated UV-B radiation and the addition of selenium on common and tartary buckwheat[J]. Photosynthetica,2005,43(4):583-589.
[8]杨璐,赵天宏. UV-B辐射增强对大豆根系活性氧代谢及抗氧化系统的影响[J].华北农学报,2018,33(5):174-180.
[9]顾自奋,黄志仁,许如根,等.近10年世界大麦生产概况[J].大麦科学,2001(1):1-4.
[10]武君,娄运生,李永秀,等. UV-B辐射增强对大麦生理生态的影响[J].农业环境科学学报,2010,29(6):1033-1038.
[11]娄运生,曾志平,韩艳,等. UV-B增强下施钾对大麦抽穗期生理特性日变化的影响[J].土壤,2014,46(2):250-255.
[12]张义贤,李晓科.镉、铅及其复合污染对大麦幼苗部分生理指标的影响[J].植物研究,2008,28(1):43-46.
[13]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:258-261.
[14]潘瑞炽.植物生理学[M].北京:高等教育出版社,2012:74-81.
[15]徐佳妮,雷梦琦,鲁瑞琪,等. UV-B辐射增强对植物影响的研究进展[J].基因组学与应用生物学,2015,34(6):1347-1352.
[16]李昌亨,高美英,张鹏飞,等. UV-C照射对赤霞珠叶片抗氧化酶系的影响[J].山西农业科学,2014,42(4):332-334,337.
[17]吴济南,王丽玲,王惟帅,等.阿特拉津和乙草胺混用对夏玉米叶片生理指标的影响[J].河南农业科学,2011,40(8):142-144.
[18]张义贤,付亚萍,肖志华,等.铅胁迫对不同基因型谷子幼苗生理特性及基因组DNA多态性的影响[J].农业环境科学学报,2013,32(3):478-484.
[19]晏斌,戴秋杰.紫外线B对水稻叶组织中活性氧代谢及膜系统的影响[J].植物生理学报,1996,22(4):373-378.
[20]张新永,郭华春,艾星梅.增强UVB辐射对彩色马铃薯相关生理生化特性的影响[J].生态环境学报,2009,18(2):664-668.
[21]莫凌,黄玉清,桂堂辉,等. 5个热带禾本科牧草品种的光合-光响应特性研究[J].草业科学,2010,27(9):64-68.
[22]胡晓雪,杜维俊,杨珍平.大气CO2浓度和气温升高对野生大豆光合作用的影响[J].山西农业科学,2015,43(7):798-801,853.
[23]韩世洁,董宽虎,王朋.不同居群白羊草光合特性比较[J].草业科学,2018,35(3):597-605.
[24]郑国琦,许兴,徐兆桢,等.盐胁迫对枸杞光合作用的气孔与非气孔限制[J].西北植物学报,2002,22(6):1355-1359.
[25]董杰,陈新新,杨倩.高光、水分和盐胁迫下小麦光合特性和抗氧化酶系统的比较[J].麦类作物学报,2018,38(3):315-322.
[26]时丽冉,崔兴国,朱云霞.盐胁迫对不同品种小黑麦光合特性和生长的影响[J].衡水学院学报,2009,11(4):61-64.
[27]黄梅玲,江洪,金清,等. UV-B辐射胁迫下不同起源时期的3种木本植物幼苗的生长及光合特性[J].生态学报,2010,30(8):1998-2009.
[28]石盼盼,宋朝鹏,贺帆,等.不同光质对烤烟旺长期生长发育和光合特性的影响[J].山西农业科学,2013,41(1):36-39,56.
[29]马野,原换换.干旱胁迫下外源油菜素内酯对玉米幼苗光合作用和D1蛋白的调控效应[J].华北农学报,2017,32(3):118-124.