天冬氨酸-β-环糊精对芴-镉复合污染土壤酶活性及微生物种群的影响
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摘要
为了评估天冬氨酸-β-环糊精(ACD)对土壤环境的影响,以土壤中微生物种群和酶活性为研究对象,利用平板计数和酶活性测定方法,对ACD作用下的芴和镉复合污染土壤中微生物种群数量和土壤酶活性进行了分析.结果表明:2%ACD作用于芴和镉复合污染土壤后,对于100 mg·kg~(-1)芴和15 mg·kg~(-1)镉的复合污染中脲酶和多酚氧化酶的活性分别增加26.85%和35.40%,且污染土壤中细菌、真菌、放线菌数量分别增加43.86%、35.40%和70.96%,ACD对复合污染土壤中酶活性的改善和微生物种群数量的提高有明显的促进效果,可以有效改善土壤的生态环境.
In order to evaluate the effect of aspartic acid-β-cyclodextrin(ACD) on the soil environment, the microbial populations and soil enzyme activities, cadmium and fluorine co-contaminated soil were studied. The number of main microbial populations and the enzyme activities of the soil contaminated with 100 mg·kg~(-1) fluorine and 15 mg· kg~(-1) cadmiumin at the presence of ACD were analyzed by plate colony counting and the enzyme assay. The results showed that with 2% ACD being added to the contaminated soil, the activity of urease and polyphenol oxidase increased by 26.85% and 35.40%, respectively, the number of bacteria, fungi and actinomycetes in contaminated soil increased by 43.86 %, 35.40 % and 70.96%, respectively. It could be concluded that ACD has evident accelerating effect on the improvement of enzyme activities and microbial populations in soil, which may improve the soil environment.
In order to evaluate the effect of aspartic acid-β-cyclodextrin(ACD) on the soil environment, the microbial populations and soil enzyme activities, cadmium and fluorine co-contaminated soil were studied. The number of main microbial populations and the enzyme activities of the soil contaminated with 100 mg·kg~(-1) fluorine and 15 mg· kg~(-1) cadmiumin at the presence of ACD were analyzed by plate colony counting and the enzyme assay. The results showed that with 2% ACD being added to the contaminated soil, the activity of urease and polyphenol oxidase increased by 26.85% and 35.40%, respectively, the number of bacteria, fungi and actinomycetes in contaminated soil increased by 43.86 %, 35.40 % and 70.96%, respectively. It could be concluded that ACD has evident accelerating effect on the improvement of enzyme activities and microbial populations in soil, which may improve the soil environment.
引文
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[15]徐兰,胡苏杭,章绍康,等.天冬氨酸-β-环糊精对土壤中芴和镉的分配行为研究[J].环境污染与防治,2016,38(7):56-60.Xu L,Hu S H,Zhang S K,et al.Distribution behavior of fluorene and cadmium in soils by aspartic acid-β-cyclodextrin[J].Environmental Pollution&Control,2016,38(7):56-60.
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[18]杜春梅,董锡文,吴玉德,等.长期旱改水对黑土微生物类群及酶活性的影响[J].北方园艺,2018,42(4):127-132.Du C M,Dong X W,Wu D Y,et al.Effects of longterm turning dry land into paddy field on microbial groups and enzyme activities in black soil[J].Northern Horticulture,2018,42(4):127-132.
[19]Del Valle E M M.Cyclodextrins and their uses:a review[J].Process Biochemistry,2004,39(9):1 033-1 046.DOI:10.1016/S0032-9592(03)00258-9.
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[21]徐兰,李青,章绍康,等.环糊精及其衍生物对降低铅和菲的藻类毒性研究[J].环境污染与防治,2016,38(9):66-72.Xu L,Li Q,Zhang S K,et al.Effects of cyclodextrin and its derivatives on decreasing toxicity of Pb and phenanthrene to algae[J].Environmental Pollution&Control,2016,38(9):66-72.
[22]高大翔,郝建朝,金建华,等.重金属汞、镉单一胁迫及复合胁迫对土壤酶活性的影响[J].农业环境科学学报,2008,27(3):903-908.DOI:10.3321/j.issn:1672-2043.2008.03.013.Gao D X,Hao J C,Jing J H,et al.Effects of single stress and combined stress of Hg and Cd on soil enzyme activities[J].Journal of Agro-Environment Science,2008,27(3):903-908.
[23]Tribak M,Ocampo J A,Garc1a-Romera I.Production of xyloglucanolytic enzymes by Trichoderma viride,Paecilomyces farinosus,Wardomyces inflatus,and Pleurotus ostreatus[J].Mycologia,2002,94(3):404-410.DOI:10.1080/15572536.2003.11833205.
[2]Dixit R,Malaviya D,Pandiyan K,et al.Bioremediation of heavy metals from soil and aquatic environment:an overview of principles and criteria of fundamental processes[J].Sustainability,2015,7(2):2 189-2 212.DOI:10.3390/su7022189.
[3]孔龙,谭向平,和文祥,等.外源Cd对中国不同类型土壤酶活性的影响[J].中国农业科学,2013,46(24):5 150-5 162.DOI:10.3864/j.issn.0578-1752.2013.24.009.Kong L,Tan X P,He W X,et al.Response of soil enzyme activity in different type of soils to cadmium exposure in China[J].Scientia Agricultura Sinica,2013,46(24):5 150-5 162.
[4]Binet P,Portal J M,Leyval C.Dissipation of 3~6 ring polycyclic aromatic hydrocarbons in the rhizosphere of ryegrass[J].Soil Biology and Biochemistry,2001,32(24):2 011-2 017.
[5]和文祥,陈会明.汞铬砷元素污染土壤的酶监测研究[J].环境科学学报,2000,20(3):338-343.He W X,Chem H M.Study on enzyme index in soils polluted by mercury,chromium and arsenic[J].Acta Scientiae Circumstantiae,2000,20(3):338-343.
[6]朱凡,洪湘琦,闫文德,等.PAHs污染土壤植物修复对酶活性的影响[J].生态学报,2014,34(3):581-588.Zhu F,Hong X Q,Yan W D,et al.Enzymatic activity during phytoremediation of polycyclic aromatic hydrocarbon impacted soil[J].Acta Ecologica Sinica,2014,34(3):581-588.
[7]Yang C J,Zhou Q X,Wei S H,et al.Chemical-assisted phytoremediation of Cd-PAHs contaminated soils using Solanum nigrum L.[J].International Journal of Phytoremediation,2011,13(8):818-833.DOI:10.1080/15226514.2010.532179.
[8]王光辉,黄磊,于荣,等.β-环糊精对双酚A分子识别作用的表征研究[J].云南大学学报:自然科学版,2008,30(6):606-610.DOI:10.3321/j.issn:0258-7971.2008.06.013.Wang G H,Huang L,Yu R,et al.Study on characterization of molecular recognition of interaction of bisphenol A byβ-cyclodextrin[J].Journal of Yunnan University:Natural Sciences Edition,2008,30(6):606-610.
[9]李海丰,王光辉,于荣,等.环糊精在环境治理中的应用研究进展[J].环境污染与防治,2007,29(11):844-847.DOI:10.3969/j.issn.1001-3865.2007.11.012.Li H F,Wang G H,Yu R,et al.Literature review on the uses of cyclodextrins in environmental control applications[J].Environmental Pollution and Control,2007,29(11):844-847.
[10]Wang G H,Zhou Y M,Wang X G,et al.Simultaneous removal of phenanthrene and lead from artificially contaminated soils with glycine-β-cyclodextrin[J].Journal of Hazardous Materials,2010,184(1-3):690-695.DOI:10.1016/j.jhazmat.2010.08.094.
[11]Wang G H,Xu W D,Wang X G,et al.Glycine-β-cyclodextrin enhanced electrokinetic removal of atrazine from contaminated soils[J].Environmental Engineering Science,2012,29(6):406-411.DOI:10.1089/ees.2010.0272.
[12]Wang G H,Wang Y,Deng N S,et al.Cysteine-β-cyclodextrin enhanced phytoremediation of soil co-contaminated with phenanthrene and lead[J].Environmental Science and Pollution Research,2015,22(13):10 107-10 115.DOI:10.1007/s11356-015-4210-7.
[13]Wang G H,Jiang Y,Hu S H,et al.Aspartic acid-β-cyclodextrin-assisted phytoremediation of soil cocontaminated with cadmium and fluorene using Alfalfa(Medicago sativa L.)[J].Environmental Engineering Science,2018,35(4):278-288.
[14]徐兰.天冬氨酸-β-CD对重金属和有机污染物毒性及酶和微生物活性影响研究[D].南昌:东华理工大学,2016.Xu L.Study on effect of aspartic acid-β-CD on toxicities of heavy metals and organic pollutants,enzyme and microbial activity[D].Nanchang:East China University of Technology.2016
[15]徐兰,胡苏杭,章绍康,等.天冬氨酸-β-环糊精对土壤中芴和镉的分配行为研究[J].环境污染与防治,2016,38(7):56-60.Xu L,Hu S H,Zhang S K,et al.Distribution behavior of fluorene and cadmium in soils by aspartic acid-β-cyclodextrin[J].Environmental Pollution&Control,2016,38(7):56-60.
[16]李振高,骆永明,腾应.土壤与环境微生物研究法[M].北京:科学出版社,2008:52-69.Li Z G,Luo Y M,Teng Y.Soil and environmental microbiology[M].Beijing:Science Press,2008:52-69.
[17]关松荫.土壤酶及其研究法[M].北京:农业出版社,1986:274-339.Guan X Y.Soil enzyme and its method[M].Beijing:Agriculture Press,1986:274-339.
[18]杜春梅,董锡文,吴玉德,等.长期旱改水对黑土微生物类群及酶活性的影响[J].北方园艺,2018,42(4):127-132.Du C M,Dong X W,Wu D Y,et al.Effects of longterm turning dry land into paddy field on microbial groups and enzyme activities in black soil[J].Northern Horticulture,2018,42(4):127-132.
[19]Del Valle E M M.Cyclodextrins and their uses:a review[J].Process Biochemistry,2004,39(9):1 033-1 046.DOI:10.1016/S0032-9592(03)00258-9.
[20]Luo W S,D'Angelo E M,Coyne M S.Plant secondary metabolites,biphenyl and hydroxypropyl-β-cyclodextrin effects on aerobic polychlorinated biphenyl removal and microbial community structure in soils[J].Soil Biology and Biochemistry,2007,39(3):735-743.DOI:10 .1016/j.soilbio.2006.09.019.
[21]徐兰,李青,章绍康,等.环糊精及其衍生物对降低铅和菲的藻类毒性研究[J].环境污染与防治,2016,38(9):66-72.Xu L,Li Q,Zhang S K,et al.Effects of cyclodextrin and its derivatives on decreasing toxicity of Pb and phenanthrene to algae[J].Environmental Pollution&Control,2016,38(9):66-72.
[22]高大翔,郝建朝,金建华,等.重金属汞、镉单一胁迫及复合胁迫对土壤酶活性的影响[J].农业环境科学学报,2008,27(3):903-908.DOI:10.3321/j.issn:1672-2043.2008.03.013.Gao D X,Hao J C,Jing J H,et al.Effects of single stress and combined stress of Hg and Cd on soil enzyme activities[J].Journal of Agro-Environment Science,2008,27(3):903-908.
[23]Tribak M,Ocampo J A,Garc1a-Romera I.Production of xyloglucanolytic enzymes by Trichoderma viride,Paecilomyces farinosus,Wardomyces inflatus,and Pleurotus ostreatus[J].Mycologia,2002,94(3):404-410.DOI:10.1080/15572536.2003.11833205.