骨组织再生工程中富血小板血浆的应用与研究进展
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摘要
背景:研究发现血小板浓缩物是一种自体全血离心后获得的富集大量血小板的血浆,具有促进组织愈合的能力,而富血小板血浆是其第1代血小板浓缩物,研究表明其具有诱导成骨效应。目的:阐述富血小板血浆的制备、分类、血小板源生长因子的释放及其促进骨再生作用及临床应用,为进一步研究富血小板血浆在骨组织再生工程的作用及临床应用提供一定的理论基础。方法:以"platelet-rich plasma,bone defect,bone regeneration,growth factors"及"富血小板血浆,骨缺损,骨再生,生长因子"为检索词,应用计算机在CNKI、PubMed数据库检索1970年7月至2018年7月相关文献共136篇。对富血小板血浆的制备、分类、血小板源生长因子的释放及其促进骨再生作用及临床应用进行综述。结果与结论:富血小板血浆是通过控制离心来获得不同浓度血小板的血浆制品,其来源于外周血,具有来源丰富、取材方便、制备简单和可吸收的优点。富血小板血浆不仅具有止血的作用,而且含有多种高浓度的生长因子及纤维蛋白,为修复细胞和组织提供良好的生长环境和支架,还刺激软组织再生,促进伤口早期闭合,以及多种炎性因子调控损伤修复的环境,还可防止感染。富血小板血浆可加快修复损伤或骨缺损,联合其他疗法应用于再生医学中为帮助愈合、缩短愈合时间提供了新的思路,尤其是在骨、软骨和肌腱修复方面有着广泛的应用前景。
BACKGROUND: Platelet concentrates have been found to be a kind of platelet-rich plasma obtained by centrifugation of autologous whole blood, and has the ability to promote tissue healing. Platelet-rich plasma, as the first generation of platelet concentrates, can facilitate osteogenesis.OBJECTIVE: To review the preparation, and classification of platelet-rich plasma, release of platelet-derived growth factor and its promoting function and clinical application of bone regeneration, so as to provide theoretical basis for further investigation on the effect and clinical application of platelet-rich plasma in bone regeneration.METHODS: CNKI and Pub Med databases were retrieved using the keywords of "platelet-rich plasma, bone defect, bone regeneration, growth factors" in English and Chinese, respectively for the articles published from July 1970 to July 2018. Totally 136 articles were searched. The preparation and classification of platelet-rich plasma, the release of platelet-derived growth factor and promote bone regeneration effect and clinical applications were summarized.RESULTS AND CONCLUSION: Platelet-rich plasma is a plasma product with different concentrations of platelets obtained by controlling centrifugation. It is derived from peripheral blood and has the advantages of abundant source, convenient material, simple preparation and absorbability. Platelet-rich plasma not only has the effect of hemostasis, but contains various high concentrations of growth factors and fibrin,which provides a beneficial growth environment and scaffold for cells and tissues. Moreover, it can stimulate soft tissue regeneration, promote early closure of wound, and provides the environment for a variety of inflammatory factor repair and prevents infection. Platelet-rich plasma can accelerate the repair of damaged or bone defects. Combined with other therapies in regenerative medicine, it provides new ideas for promoting healing and shortening healing time, especially has a broad prospect in the repair of bone, cartilage and tendon.
BACKGROUND: Platelet concentrates have been found to be a kind of platelet-rich plasma obtained by centrifugation of autologous whole blood, and has the ability to promote tissue healing. Platelet-rich plasma, as the first generation of platelet concentrates, can facilitate osteogenesis.OBJECTIVE: To review the preparation, and classification of platelet-rich plasma, release of platelet-derived growth factor and its promoting function and clinical application of bone regeneration, so as to provide theoretical basis for further investigation on the effect and clinical application of platelet-rich plasma in bone regeneration.METHODS: CNKI and Pub Med databases were retrieved using the keywords of "platelet-rich plasma, bone defect, bone regeneration, growth factors" in English and Chinese, respectively for the articles published from July 1970 to July 2018. Totally 136 articles were searched. The preparation and classification of platelet-rich plasma, the release of platelet-derived growth factor and promote bone regeneration effect and clinical applications were summarized.RESULTS AND CONCLUSION: Platelet-rich plasma is a plasma product with different concentrations of platelets obtained by controlling centrifugation. It is derived from peripheral blood and has the advantages of abundant source, convenient material, simple preparation and absorbability. Platelet-rich plasma not only has the effect of hemostasis, but contains various high concentrations of growth factors and fibrin,which provides a beneficial growth environment and scaffold for cells and tissues. Moreover, it can stimulate soft tissue regeneration, promote early closure of wound, and provides the environment for a variety of inflammatory factor repair and prevents infection. Platelet-rich plasma can accelerate the repair of damaged or bone defects. Combined with other therapies in regenerative medicine, it provides new ideas for promoting healing and shortening healing time, especially has a broad prospect in the repair of bone, cartilage and tendon.
引文
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