回顾性研究证明中国猪圆环病毒3型存在不同进化分支
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摘要
为研究猪圆环病毒3型(PCV3)在我国的流行分布情况,以及对PCV3毒株的遗传进化进行分析,笔者利用PCR检测方法对2006年-2017年采集的猪组织和血清样本进行了PCV3检测,并进一步对全基因序列进行了扩增和测序,对基因序列和氨基酸序列进行比对分析并绘制遗传进化树。结果表明,PCV3在我国各地区猪场广泛存在并传播,且近期分离到的PCV3毒株与早期的PCV3分离株有明显差异,证明了PCV3病毒在不断进化。该研究结果揭示了PCV3的进化趋势,并提示迫切需要对PCV3进行更为广泛的流行病学监测和致病性评估。
The objective of this study was to investigate the occurrence and polygenetic analysis of Porcine circovirus type 3(PCV3) in China. PCV3 virus was detected in pig tissue and blood samples collected from 2006 to 2017 via PCR detection method, complete genome of PCV3 was further amplified and sequenced, and the gene sequence and amino acid sequence were analyzed by phylogenetic tree. The result suggest that PCV3 was widely distributed and spread in pig farms in various regions of China. The PCV3 stain recently isolated was significantly different from the early PCV3 isolates, which suggested that PCV3 virus was undergoing continuous evolution. Our findings suggested the evolution of PCV3 and highlighted the need for extensive epidemiological surveillance and pathogenicity evaluation.
The objective of this study was to investigate the occurrence and polygenetic analysis of Porcine circovirus type 3(PCV3) in China. PCV3 virus was detected in pig tissue and blood samples collected from 2006 to 2017 via PCR detection method, complete genome of PCV3 was further amplified and sequenced, and the gene sequence and amino acid sequence were analyzed by phylogenetic tree. The result suggest that PCV3 was widely distributed and spread in pig farms in various regions of China. The PCV3 stain recently isolated was significantly different from the early PCV3 isolates, which suggested that PCV3 virus was undergoing continuous evolution. Our findings suggested the evolution of PCV3 and highlighted the need for extensive epidemiological surveillance and pathogenicity evaluation.
引文
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[2] Cao L,Sun WC,Lu HJ,et al.Genetic variation analysis of PCV1 strains isolated from Guangxi province of China in2015[J].BMC Vet Res,2018,14(1):43.
[3] Meng XJ.Porcine circovirus type 2(PCV2):pathogenesis and interaction with the immune system[J].Annu Rev Anim Biosci,2013(1):43-64.
[4] Baekbo P,Kristensen CS,Larsen LE.Porcine circovirus diseases:a review of PMWS[J].Transbound Emerg Dis,2012,59(Suppl1):60-67.
[5] Klausmann S,Sydler T,Summerfield A,et al.T-cell reprogramming through targeted CD4-coreceptor and T-cell receptor expression on maturing thymocytes by latent Circoviridae family member porcine cir covirus type 2 cell infections in the thymus[J].Emerg Microbes Infect,2015,4(3):e15.
[6] Zhu SS,Zhang CY,Jing W,et al.Immunity elicited by an experimental vaccine based on recombinant flagellin-porcine circovirus type 2 cap fusion protein in piglets[J].PLo S One,2016,11(2):e0147432.
[7] Palinski R,Pineyro P,Shang P,et al.A novel porcine circovirus distantly related to known circoviruses is associated with porcine dermatitis and nephropathy syndrome and reproductive failure[J].J Virol,2017,91(1).
[8] Phan TG,Giannitti F,Rossow S,et al.Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation[J].Virol J,2016,13(1):184.
[9] Wen S,Sun W,Li Z,et al.The detection of porcine circovirus3 in Guangxi,China[J].Transbound Emerg Dis,2018,65(1):27-31.
[10] Tochetto C,Lima DA,Varela AP,et al.Full-genome sequence of porcine circovirus type 3 recovered from serum of sows with stillbirths in Brazil[J].Transbound Emerg Dis,2018,65(1):5-9.
[11] Kedkovid,R,Woonwong Y,Arunorat J,et al.Porcine circovirus type 3(PCV3)infection in grower pigs from a Thai farm suffering from porcine respiratory disease complex(PRDC)[J].Vet Microbiol,2018(215):71-76.
[12] Stadejek T,Wozniak A,Milek D,et al.First detection of porcine circovirus type 3 on commercial pig farms in Poland[J].Transbound Emerg Dis,2017,64(5):1350-1353.
[13] Kwon T,Yoo SJ,Park CK,et al.Prevalence of novel porcine circovirus 3 in Korean pig populations[J].Vet Microbiol,2017,207:178-180.
[14] Faccini S,Barbieri I,Gilioli A,et al.Detection and genetic characterization of porcine circovirus type 3 in Italy[J].Transbound Emerg Dis,2017,64(6):1661-1664.
[15] Fux,R,Sockler C,Renken C,et al,Full genome characterization of porcine circovirus type 3 isolates reveals the existence of two distinct groups of virus strains[J].Virol J,2018,15(25):2-9.
[16] Franzo G,Legnardi M,Hjulsager CK,et al.Full-genome sequencing of porcine circovirus 3 field strains from Denmark,Italy and Spain demonstrates a high within-Europe genetic heterogeneity[J].Transbound Emerg Dis,2018,65(3):602-606.
[17] Ku X,Chen F,Li P,et al.Identification and genetic characterization of porcine circovirus type 3 in China[J].Transbound Emerg Dis,2017,64(3):703-708.
[18] Fu X,Fang B,Ma J,et al.Insights into the epidemic characteristics and evolutionary history of the novel porcine circovirus type 3 in southern China[J].Transbound Emerg Dis,2018,65(2):e296-e303.
[19] Oliver-Ferrando S,Segales J,Lopez-Soria S,et al.Evaluation of natural porcine circovirus type 2(PCV2)subclinical infection and seroconversion dynamics in piglets vaccinated at different ages[J].Vet Res,2016,47(1):121.
[20] Schuh AJ,Ward MJ,Brown AJL,et al.Dynamics of the emergence and establishment of a newly dominant genotype of Japanese encephalitis virus throughout Asia[J].J Virol,2014,88(8):4522-4532.
[21] Lung O,Pasick,J,Fisher M,et al.Insulated isothermal reverse transcriptase PCR(ii RT-PCR)for rapid and sensitive detection of classical swine fever virus[J].Transbound Emerg Dis,2016,63(5):E395-E402.
[22] Zhou XR,Zhang TS,Song DP,et al.Comparison and evaluation of conventional RT-PCR,SYBR green I and Taq Man real-time RT-PCR assays for the detection of porcine epidemic diarrhea virus[J].Mol Cell Prob,2017(33):36-41.
[23] Yuan WZ,Wang JC,Sun MT,et al.Rapid detection of encephalomyocarditis virus by one-step reverse transcription loop-mediated isothermal amplification method[J].Virus Res,2014(189):75-78.
[24] Zeng ZY,Liu ZJ,Wang WC,et al.Establishment and application of a multiplex PCR for rapid and simultaneous detection of six viruses in swine[J].J Virol Method,2014(208):102-106.
[25] Pieters M,Daniels J,Rovira A.Comparison of sample types and diagnostic methods for in vivo detection of Mycoplasma hyopneumoniae during early stages of infection[J].Vet Microbiol,2017(203):103-109.
[26] Zhou L,Zhang JL,Zeng JW,et al.The 30-Amino-Acid Deletion in the Nsp2 of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China is not related to its virulence[J].J Virol,2009,83(10):5156-5167.
[27] An TQ,Tian ZJ,Leng CL,et al.Highly pathogenic porcine reproductive and respiratory syndrome virus,Asia[J].Emerg Infect Dis,2011,17(9):1782-1784.
[28] Huong VTL,Thanh LV,Phu VD,et al.Temporal and spatial association of Streptococcus suis infection in humans and porcine reproductive and respiratory syndrome outbreaks in pigs in northern Vietnam[J].Epidemiol Infect,2016,144(1):35-44.