狐尾藻乙酸乙酯萃取部位总黄酮含量测定与抗氧化活性研究

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英文篇名：Determination of Total Flavonoid Content and Antioxidant Activity of Myriophyllum Verticillatum L Ethyl Acetate Extraction Site 作者：陈春雅 ; 毛玉玲 ; 李泽宁 ; 许益迪 ; 潘奇献 ; 徐润生 英文作者：CHEN Chun-ya;MAO Yu-ling;LI Ze-ning;XU Yi-di;PAN Qi-xian;XU Run-sheng;Jiyang College, Zhejiang Agricultural and Forestry University; 关键词：狐尾藻 ; 乙酸乙酯萃取部位 ; 总黄酮 ; 含量测定 ; 抗氧化活性 英文关键词：Myriophyllum verticillatum L.;;ethyl acetate extraction site;;total flavonoids;;content determination;;antioxidant activity 中文刊名：ZJHG 英文刊名：Zhejiang Chemical Industry 机构：浙江农林大学暨阳学院; 出版日期：2019-03-15 出版单位：浙江化工 年：2019 期：v.50 基金：绍兴市大学生科技创新项目(SXSDC201735) 语种：中文; 页：ZJHG201903013 页数：4 CN：03 ISSN：33-1093/TQ 分类号：46-49

摘要

目的:以浙江省采集的狐尾藻作为研究对象,将狐尾藻植物乙醇提取的浸膏用乙酸乙酯进行萃取,对所得乙酸乙酯部位进行抗氧化活性测定。为狐尾藻的开发利用提供科学依据。方法:通过比色法对狐尾藻乙酸乙酯部位总黄酮总酚酸含量进行测定,采用DPPH法、羟自由基法测定狐尾藻乙酸乙酯部位的抗氧化活性。结果:狐尾藻乙酸乙酯部位含有黄酮类化合物,且样品溶液具有较好的抗氧化活性,在一定浓度范围内,样品浓度越高,其抗氧化活性越强。结论:该研究结果表明狐尾藻中含有黄酮类化合物,且在一定范围内其抗氧化活性比Trolox强。
        Objective: To extract the ethanol extract of Myriophyllum verticillatum L. from Zhejiang Province by ethyl acetate and extract the acetic acid.The antioxidant activity of ethyl acetate was provided as a scientific basis for the development and utilization of Myriophyllum verticillatum L. Methods: The total flavonoid content of total flavonoids in the ethyl acetate fraction of Myriophyllum verticillatum L. flavus was determined by colorimetry. DPPH method and hydroxyl radical method were used to determine the antioxidant activity of the ethyl acetate fraction of Myriophyllum verticillatum L.. Results: The ethyl acetate fraction of Myriophyllum verticillatum L.. flavus contained flavonoids.And the sample solution has good antioxidant activity, in a certain concentration range, the higher the concentration of the sample, the stronger the antioxidant activity. Conclusions: The results showed that there are flavonoids in the Myriophyllum verticillatum L. and in a certain range, its antioxidant activity was stronger than Trolox..

引文

[1]李益健.淡水藻类的经济意义[J].生物学通报, 1982,(5):3-5,28.
    [2]祝寅淏,王帅,李瑶,等.黄酮类化合物药理作用的研究进展[J].吉林医药学院学报,2018,(3):219-223.
    [3]刘勇弟,徐寿昌.紫外-Fenton试剂的作用机理及在废水处理中的应用[J].环境化学, 1994,(4):302-306.
    [4]曾燕,郭兰萍,王继永,等.植物黄酮类化合物的化学生态学功能及中药材定向栽培的构想[J].中国现代中药,2015,17(8):776-790.
    [5]左玉.自由基、活性氧与疾病[J].粮食与油脂,2011,(9):9-11.