一株光合细菌的分离筛选及其对无机氮的去除能力
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摘要
通过对海洋环境污泥进行富集培养及分离筛选得到一株光合细菌,通过16S rDNA全序列分析,结合菌株形态和结构,鉴定其为Ectothiorhodospira magna。研究表明菌株在盐度30‰、28℃、DO 8 mg/L的条件下,对初始浓度分别为280、84、98 mg/L的氨氮、亚硝酸盐氮和硝酸盐氮经过10 d处理的去除率分别为81.83%、46.21%、86.79%。在氨氮、亚硝酸盐氮和硝酸盐氮共存的环境下,菌株首先利用氨氮,之后将亚硝酸盐氮转化成硝酸盐氮。
By enriching the marine environmental sludge,a strain of photosynthetic bacterium was isolated,of which the 16 S rDNA sequence was constructed. Combined with the morphology of the strain,the strain was initially identified to Ectothiorhodospira magna. Its removal efficiencies of ammonia nitrogen,nitrite nitrogen,and nitrate nitrogen were studied under the conditions of salinity 30‰,28 ℃,and DO 8 mg/L. Under artificial seawater conditions,within 10 days,the removal rates of 280 mg/L ammonia nitrogen,84 mg/L nitrite nitrogen,and 98 mg/L nitrate nitrogen were 81.83%,46.21%,and 86.79%,respectively. In the environment where ammonia nitrogen,nitrite and nitrate coexist,the strain first removes ammonia nitrogen and converts it into nitrite and then converts nitrite into nitrate.
By enriching the marine environmental sludge,a strain of photosynthetic bacterium was isolated,of which the 16 S rDNA sequence was constructed. Combined with the morphology of the strain,the strain was initially identified to Ectothiorhodospira magna. Its removal efficiencies of ammonia nitrogen,nitrite nitrogen,and nitrate nitrogen were studied under the conditions of salinity 30‰,28 ℃,and DO 8 mg/L. Under artificial seawater conditions,within 10 days,the removal rates of 280 mg/L ammonia nitrogen,84 mg/L nitrite nitrogen,and 98 mg/L nitrate nitrogen were 81.83%,46.21%,and 86.79%,respectively. In the environment where ammonia nitrogen,nitrite and nitrate coexist,the strain first removes ammonia nitrogen and converts it into nitrite and then converts nitrite into nitrate.
引文
[1] 卢海凤,张光明,赵微.光合细菌污水资源化技术[M].北京:中国建筑工业出版社,2015,1.
[2] Holt J H,Bergey D H,Krieget N R,et al.Bergey's manual of determinative bacteriology[M].Williams Wilkins Company,1994:520-521.
[3] Kobayashi M,chan Y T.Treatment of Industrial Waste Solutions and Production of Useful By-Products Using a Photosynthetic Bacterial Method[J].Water Research,1973,7(8):1219-1224.
[4] Bryantseva I A,Tourova T P,Kovaleva O L,et al.Ectothiorhodospira magna sp.nov.,a New Large Alkaliphilic Purple Sulfur Bacterium[J].Microbiology,2010,79(6):780-790.
[5] 卢海凤,张光明,何春华.一株光合细菌的鉴定及其处理大豆加工废水试验[J].哈尔滨工业大学学报,2011.
[6] 水和废水检测分析方法(第四版)[M].北京:中国环境科学出版社,2002:266-268,279-281.
[7] 闫坤朋,牛成洁,宋志文,等.重氮偶联法测定水中亚硝酸盐氮的改进[J].中国环境监测,2018.
[8] Oren A,The Family Ectothiorhodospiraceae//Rosenberg E,DeLong E F,Lory S,et al.The Prokaryotes Gammaproteobacteria 4th ed[M].Springer Heidelberg New York Dordrecht London.199-222.
[9] Gorlenko V M,Bryantseva I A,Rabold S,et al.Ectothiorhodospira variabilis sp.nov.,an alkaliphilic and halophilic purple sulfur bacterium from soda lakes[J].Int J Syst Evol Microbiol,2009,59:658-664.
[2] Holt J H,Bergey D H,Krieget N R,et al.Bergey's manual of determinative bacteriology[M].Williams Wilkins Company,1994:520-521.
[3] Kobayashi M,chan Y T.Treatment of Industrial Waste Solutions and Production of Useful By-Products Using a Photosynthetic Bacterial Method[J].Water Research,1973,7(8):1219-1224.
[4] Bryantseva I A,Tourova T P,Kovaleva O L,et al.Ectothiorhodospira magna sp.nov.,a New Large Alkaliphilic Purple Sulfur Bacterium[J].Microbiology,2010,79(6):780-790.
[5] 卢海凤,张光明,何春华.一株光合细菌的鉴定及其处理大豆加工废水试验[J].哈尔滨工业大学学报,2011.
[6] 水和废水检测分析方法(第四版)[M].北京:中国环境科学出版社,2002:266-268,279-281.
[7] 闫坤朋,牛成洁,宋志文,等.重氮偶联法测定水中亚硝酸盐氮的改进[J].中国环境监测,2018.
[8] Oren A,The Family Ectothiorhodospiraceae//Rosenberg E,DeLong E F,Lory S,et al.The Prokaryotes Gammaproteobacteria 4th ed[M].Springer Heidelberg New York Dordrecht London.199-222.
[9] Gorlenko V M,Bryantseva I A,Rabold S,et al.Ectothiorhodospira variabilis sp.nov.,an alkaliphilic and halophilic purple sulfur bacterium from soda lakes[J].Int J Syst Evol Microbiol,2009,59:658-664.