基于普鲁士蓝纳米立方的类过氧化物酶活性检测还原型谷胱甘肽
|
摘要
本研究开发了一种基于普鲁士蓝纳米立方体(PB NCs)类过氧化物酶活性检测还原型谷胱甘肽(GSH)的方法.PB NCs具有类过氧化物酶活性,可催化过氧化氢氧化3,3',5,5'-四甲基联苯胺(TMB)生成氧化态的TMB(oxTMB),在652nm处有较强的紫外-可见吸收峰;GSH既可以与PB NCs表面Fe3+结合,又可还原oxTMB,这双重作用抑制了TMB的氧化过程,引起体系紫外-可见吸收峰强度降低.基于体系紫外-可见吸收峰强度随GSH浓度的变化关系,我们得到GSH检测线性范围为0.01-5.0μmol/L.该方法具有较高的灵敏度和较好的选择性,不仅为GSH的检测提供了一种新的思路,还拓展了PB NCs在生物分析检测中的应用.
In this paper,a novel method for glutathione detection is developed based on the peroxidaselike activity of Prussian blue nanocube(PB NCs).PB NCs catalyzes the oxidation of TMB by H2 O2 to obtain the oxidized TMB(oxTMB)with a maximum absorption at 652 nm.The introduction of glutathione can cheat with Fe3+on the surface of PB NCs and cause the reduction of oxTMB,both of which inhibit the oxidation of TMB,resulting in the decrease of the absorbance at 652 nm.Based on the relationship between the changes of absorbance and the concentration of glutathione,a linear relationship in the glutathione range from 0.01 to 5.0μmol/L is obtained.This method shows high sensitivity and good selectivity,which may not only provide a new idea for glutathione detection,but also expand the application of PB NCs in bioanalytical detection.
In this paper,a novel method for glutathione detection is developed based on the peroxidaselike activity of Prussian blue nanocube(PB NCs).PB NCs catalyzes the oxidation of TMB by H2 O2 to obtain the oxidized TMB(oxTMB)with a maximum absorption at 652 nm.The introduction of glutathione can cheat with Fe3+on the surface of PB NCs and cause the reduction of oxTMB,both of which inhibit the oxidation of TMB,resulting in the decrease of the absorbance at 652 nm.Based on the relationship between the changes of absorbance and the concentration of glutathione,a linear relationship in the glutathione range from 0.01 to 5.0μmol/L is obtained.This method shows high sensitivity and good selectivity,which may not only provide a new idea for glutathione detection,but also expand the application of PB NCs in bioanalytical detection.
引文
[1] Gao Y,Wu K L,Li H Y,et al.Glutathione detection based on peroxidase-like activity of Co3O4-Montmorillonite nanocomposites Sens[J].Actuators B,2018,273:1635-1639.
[2] Qi S J,Liu W M,Zhang P P,et al.A colorimetric and ratiometric fluorescent probe for highly selective detection of glutathione in the mitochondria of living cells[J].Sens Actuators B,2018,270:459-465.
[3] McDermott G P,Francis P S,Holt K J,et al.Determination of intracellular glutathione and glutathione disulfide using high performance liquid chromatography with acidic potassium permanganate chemiluminescence detection[J].Analyst,2011,136(12):2578-2585.
[4] Tsardaka E C,Zacharis C K,Tzanavaras P D,et al.Determination of glutathione in baker's yeast by capillary electrophoresis using methyl propiolate as derivatizing reagent[J].J Chromatogr A,2013,1300:204-208.
[5] Gong D Y,Han S C,Iqbal A,et al.Fast and selective two-stage ratiometric fluorescent probes for imaging of glutathione in living cells[J].Anal Chem,2017,89(24):13112-13119.
[6] Yuan B Q,Xu C Y,Zhang R C,et al.Glassy carbon electrode modified with 7,7,8,8-tetracyanoquinodimethane and graphene oxide triggered a synergistic effect:Low-potential amperometric detection of reduced glutathione[J].Biosens Bioelectron,2017,96:1-7.
[7] Xianyu Y L,Xie Y Z Y,Wang N X,et al.A dispersion-dominated chromogenic strategy for colorimetric sensing of glutathione at the nanomolar level using gold nanoparticles[J].Small,2015,11(41):5510-5514.
[8] Wang Q Q,Wei H,Zhang Z Q,et al.Nanozyme:An emerging alternative to natural enzyme for biosensing and immunoassay[J].TrACTrends Anal Chem,2018,105:218-224.
[9] Gao L Z,Zhuang J,Nie L,et al.Intrinsic peroxidase-like activity of ferromagnetic nanoparticles[J].Nat Nanotechnol,2007,2:577-583.
[10]Wang G L,Xu X F,Wu X M,et al.Visible-light-stimulated enzymelike activity of graphene oxide and its application for facile glucose sensing[J].J Phys Chem C,2014,118:28109-28117.
[11]Hu L Z,Yuan Y L,Zhang L,et al.Copper nanoclusters as peroxidase mimetics and their applications to H2O2and glucose detection[J].Anal Chim Acta,2013,762:83-86.
[12]Tan L L,Zhang Y M,Qiang H,et al.A sensitive Hg(II)colorimetric sensor based on synergistic catalytic effect of gold nanoparticles and Hg[J].Sens Actuators B:Chem,2016,229:686-691.
[13]Zhang L L,Han L,Hu P,et al.TiO2nanotube arrays:Intrinsic peroxidase mimetics[J].Chem Commun,2013,49:10480-10482.
[14]Lin T R,Zhong L S,Guo L Q,et al.Seeing diabetes:visual detection of glucose based on the intrinsic peroxidase-like activity of MoS2nanosheets[J].Nanoscale,2014,6:11856-11862.
[15]Lin T R,Zhong L S,Song Z P,et al.Visual detection of blood glucose based on peroxidase-like activity of WS2nanosheets[J].Biosens Bioelectron,2014,62:302-307.
[16]Ni P J,Sun Y J,Dai H C,et al.Prussian blue nanocubes peroxidase mimetic-based colorimetric assay for screening acetylcholinesterase activity and its inhibitor[J].Sens Actuators B,2017,240:1314-1320.
[17]Zhang W M,Ma D,Du J X.Prussian blue nanoparticles as peroxidase mimetics for sensitive colorimetric detection of hydrogen peroxide and glucose[J].Talanta,2014,120:362-367.
[18]Hu M,Belik A A,Imura M,et al.Tailored design of multiplenanoarchitectures in metal-cyanide hybrid coordination polymers[J].J Am Chem Soc,2013,135:384-391
[19]Li P-H,Lin J-Y,Chen C-T,et al.Using Gold Nanoclusters As Selective Luminescent Probes for Phosphate-Containing Metabolites[J].Anal Chem,2012,84(13):5484-5488.
[20]Sun Y H,Wang J,Li W,et al.DNA-stabilized bimetallic nanozyme and its application on colorimetric assay of biothiols[J].Biosens Bioelectron,2015,74:1038-1046.
[21]Xiong Y H,Chen S H,Ye F G,et al.Synthesis of a mixed valence state Ce-MOF as an oxidase mimetic for the colorimetric detection of biothiols[J].Chem Commun,2015,51(22):4635-4638.
[22]Feng J Y,Huang P C,Shi S Z,et al.Colorimetric detection of glutathione in cells based on peroxidase-like activity of gold nanoclusters:A promising powerful tool for identifying cancer cells[J].Anal Chim Acta,2017,967:64-69.
[2] Qi S J,Liu W M,Zhang P P,et al.A colorimetric and ratiometric fluorescent probe for highly selective detection of glutathione in the mitochondria of living cells[J].Sens Actuators B,2018,270:459-465.
[3] McDermott G P,Francis P S,Holt K J,et al.Determination of intracellular glutathione and glutathione disulfide using high performance liquid chromatography with acidic potassium permanganate chemiluminescence detection[J].Analyst,2011,136(12):2578-2585.
[4] Tsardaka E C,Zacharis C K,Tzanavaras P D,et al.Determination of glutathione in baker's yeast by capillary electrophoresis using methyl propiolate as derivatizing reagent[J].J Chromatogr A,2013,1300:204-208.
[5] Gong D Y,Han S C,Iqbal A,et al.Fast and selective two-stage ratiometric fluorescent probes for imaging of glutathione in living cells[J].Anal Chem,2017,89(24):13112-13119.
[6] Yuan B Q,Xu C Y,Zhang R C,et al.Glassy carbon electrode modified with 7,7,8,8-tetracyanoquinodimethane and graphene oxide triggered a synergistic effect:Low-potential amperometric detection of reduced glutathione[J].Biosens Bioelectron,2017,96:1-7.
[7] Xianyu Y L,Xie Y Z Y,Wang N X,et al.A dispersion-dominated chromogenic strategy for colorimetric sensing of glutathione at the nanomolar level using gold nanoparticles[J].Small,2015,11(41):5510-5514.
[8] Wang Q Q,Wei H,Zhang Z Q,et al.Nanozyme:An emerging alternative to natural enzyme for biosensing and immunoassay[J].TrACTrends Anal Chem,2018,105:218-224.
[9] Gao L Z,Zhuang J,Nie L,et al.Intrinsic peroxidase-like activity of ferromagnetic nanoparticles[J].Nat Nanotechnol,2007,2:577-583.
[10]Wang G L,Xu X F,Wu X M,et al.Visible-light-stimulated enzymelike activity of graphene oxide and its application for facile glucose sensing[J].J Phys Chem C,2014,118:28109-28117.
[11]Hu L Z,Yuan Y L,Zhang L,et al.Copper nanoclusters as peroxidase mimetics and their applications to H2O2and glucose detection[J].Anal Chim Acta,2013,762:83-86.
[12]Tan L L,Zhang Y M,Qiang H,et al.A sensitive Hg(II)colorimetric sensor based on synergistic catalytic effect of gold nanoparticles and Hg[J].Sens Actuators B:Chem,2016,229:686-691.
[13]Zhang L L,Han L,Hu P,et al.TiO2nanotube arrays:Intrinsic peroxidase mimetics[J].Chem Commun,2013,49:10480-10482.
[14]Lin T R,Zhong L S,Guo L Q,et al.Seeing diabetes:visual detection of glucose based on the intrinsic peroxidase-like activity of MoS2nanosheets[J].Nanoscale,2014,6:11856-11862.
[15]Lin T R,Zhong L S,Song Z P,et al.Visual detection of blood glucose based on peroxidase-like activity of WS2nanosheets[J].Biosens Bioelectron,2014,62:302-307.
[16]Ni P J,Sun Y J,Dai H C,et al.Prussian blue nanocubes peroxidase mimetic-based colorimetric assay for screening acetylcholinesterase activity and its inhibitor[J].Sens Actuators B,2017,240:1314-1320.
[17]Zhang W M,Ma D,Du J X.Prussian blue nanoparticles as peroxidase mimetics for sensitive colorimetric detection of hydrogen peroxide and glucose[J].Talanta,2014,120:362-367.
[18]Hu M,Belik A A,Imura M,et al.Tailored design of multiplenanoarchitectures in metal-cyanide hybrid coordination polymers[J].J Am Chem Soc,2013,135:384-391
[19]Li P-H,Lin J-Y,Chen C-T,et al.Using Gold Nanoclusters As Selective Luminescent Probes for Phosphate-Containing Metabolites[J].Anal Chem,2012,84(13):5484-5488.
[20]Sun Y H,Wang J,Li W,et al.DNA-stabilized bimetallic nanozyme and its application on colorimetric assay of biothiols[J].Biosens Bioelectron,2015,74:1038-1046.
[21]Xiong Y H,Chen S H,Ye F G,et al.Synthesis of a mixed valence state Ce-MOF as an oxidase mimetic for the colorimetric detection of biothiols[J].Chem Commun,2015,51(22):4635-4638.
[22]Feng J Y,Huang P C,Shi S Z,et al.Colorimetric detection of glutathione in cells based on peroxidase-like activity of gold nanoclusters:A promising powerful tool for identifying cancer cells[J].Anal Chim Acta,2017,967:64-69.