种鸡及子代肉仔鸡饲粮添加外源肌苷酸对子代肉仔鸡生长性能、肉品质及相关基因表达的影响
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摘要
本试验旨在研究种鸡及子代肉仔鸡饲粮添加外源肌苷酸(IMP)对子代肉仔鸡生长性能、肉品质及相关基因表达的影响。选取遗传背景相同、体重相近、产蛋率达到5%的20周龄健康爱拔益加(AA)种鸡864羽,随机分为2组,每组6个重复,每个重复72羽。对照组饲喂基础饲粮,试验组在基础饲粮中添加0.5%肌苷酸,试验期30 d。试验结束前1周,母鸡产蛋率到达高峰期,连续收集7 d种蛋,共3 700枚种蛋孵化,孵化结束后,依据母代分组情况,选取孵化所得1日龄健康子代肉仔鸡3 000羽,分成4组,分别记为II(母代饲喂基础饲粮+0.5%肌苷酸,子代饲喂基础饲粮+0.5%肌苷酸)、IN(母代饲喂基础饲粮+0.5%肌苷酸,子代饲喂基础饲粮)、NI(母代饲喂基础饲粮,子代饲喂基础饲粮+0.5%肌苷酸)和NN组(母代饲喂基础饲粮,子代饲喂基础饲粮),每组6个重复,每个重复125羽。试验期42 d。结果表明:1)与NN组相比,II、IN和NI组子代肉仔鸡的平均日增重、平均日采食量和料重比均无显著差异(P>0.05)。2)与NN组相比,II、IN和NI组子代肉仔鸡胸肌率和腿肌率均无显著差异(P>0.05);II组腹脂率显著高于NI组(P<0.05)。3)与NN组相比,II、IN和NI组子代肉仔鸡胸肌、腿肌的红度(a*)和黄度(b*)值均无显著差异(P>0.05),II组胸肌的亮度(L*)值显著降低(P<0.05);IN和NI组胸肌和腿肌pH45 min显著提高(P<0.05),IN和NI组胸肌pH24 h显著提高(P<0.05),II、IN和NI组腿肌pH24 h显著升高(P<0.05)。4)与NN组相比,II、IN和NI组21和42日龄胸肌和腿肌的谷氨酰胺磷酸核糖焦磷酸酰胺转移酶(GPAT)基因相对表达量没有显著差异(P>0.05),21日龄NI组胸肌和IN组腿肌的腺苷琥珀酸裂解酶(ADSL)基因相对表达量显著提高(P<0.05),42日龄II和IN组胸肌的ADSL基因相对表达量显著提高(P<0.05)。以上结果提示,种鸡及子代肉仔鸡饲粮添加外源肌苷酸对子代肉仔鸡生长性能、屠宰性能无显著影响;能够提高肌肉pH,降低胸肌L*值,提高胸肌ADSL基因表达量,在一定程度上能够提高子代肉仔鸡肉品质。
The objective of this study was to investigate the effects of adding exogenous inosinic acid(IMP) in diets of breeding hens and offspring broilers on growth performance,meat quality and related gene expression of offspring broilers.A total of 864 Arbor Acres(AA) breeding hens(20-week-old) with similar genetic background,weight and 5% egg production rate were randomly divided into 2 groups with 6 replicates in each group and 72 broilers in each replicate.Hens in the control group were fed a basic diet,and the others in the experimental group were fed the basic diet supplemented with 0.5% inosinic acid.The experiment lasted for 30 days.One week before the end of the experiment,the laying rate of hens reached the peak,a total of 3 700 eggs were continuously collected for 7 days and hatched.After hatching,a total of 3 000 one-day-old broilers were selected and divided into 4 groups according to the maternal group,named II(mother feed basal diet+0.5% inosinic acid,offspring feed basal diet+0.5% inosinic acid),IN(mother feed basal diet+0.5% inosinic acid,offspring feed basal diet),NI(mother feed basal diet,offspring feed basal diet+0.5% inosinic acid)and NN group(mother feed basal diet,offspring feed basal diet),each group had 6 replicates and 125 birds in each replicate.The experiment lasted for 42 days.The results showedas follows:1) compared with the NN group,the average daily feed intake,average daily gain and feed to gain ratio in II,IN and NI groups were no significant difference(P>0.05).2) Compared with the NN group,the breast muscle rate and leg muscle rate in II,IN and NI groups were no significant difference(P>0.05);the abdominal fat rate in II group was significantly higher than that in NI group(P<0.05).3) Compared with the NN group,the values of redness(a*) and yellowness(b*) of breast muscle and leg muscle in II,IN and NI groups were no significant difference(P>0.05),the breast muscle brightness(L*) value in II group was significantly decreased(P<0.05);the pH45 min and pH24 h of breast muscle and leg muscle in IN and NI groups were significantly increased(P<0.05);the pH24 h of breast muscle in IN and NI groups was significantly increased(P<0.05);the pH24 h of leg muscle in II,IN and NI groups was significantly increased(P<0.05).4) Compared with the NN group,the relative expression level of glutamine phosphoribosylpyrophosphate amidotransferase(GPAT) gene of breast muscle and leg muscle in II,IN and NI groups at 21 and 42 days of age was no significant difference(P>0.05),the relative expression level of adenylosuccinate lyase(ADSL) gene of breast muscle in NI group and that of leg muscle in IN group at 21 days of age was significantly increased(P<0.05),the relative expression level of ADSL gene of breast muscle in II and IN groups at 42 days of age was significantly increased(P<0.05).These results suggest that adding exogenous inosinic acid in diets of breeding hens and offspring broilers have no significant effect on growth performance and slaughter performance of offspring broilers;and can increase muscle pH,decrease breast muscle L* value,improve the relative expression of ADSL gene,and improve meat quality of offspring broilers to a certain extent.
The objective of this study was to investigate the effects of adding exogenous inosinic acid(IMP) in diets of breeding hens and offspring broilers on growth performance,meat quality and related gene expression of offspring broilers.A total of 864 Arbor Acres(AA) breeding hens(20-week-old) with similar genetic background,weight and 5% egg production rate were randomly divided into 2 groups with 6 replicates in each group and 72 broilers in each replicate.Hens in the control group were fed a basic diet,and the others in the experimental group were fed the basic diet supplemented with 0.5% inosinic acid.The experiment lasted for 30 days.One week before the end of the experiment,the laying rate of hens reached the peak,a total of 3 700 eggs were continuously collected for 7 days and hatched.After hatching,a total of 3 000 one-day-old broilers were selected and divided into 4 groups according to the maternal group,named II(mother feed basal diet+0.5% inosinic acid,offspring feed basal diet+0.5% inosinic acid),IN(mother feed basal diet+0.5% inosinic acid,offspring feed basal diet),NI(mother feed basal diet,offspring feed basal diet+0.5% inosinic acid)and NN group(mother feed basal diet,offspring feed basal diet),each group had 6 replicates and 125 birds in each replicate.The experiment lasted for 42 days.The results showedas follows:1) compared with the NN group,the average daily feed intake,average daily gain and feed to gain ratio in II,IN and NI groups were no significant difference(P>0.05).2) Compared with the NN group,the breast muscle rate and leg muscle rate in II,IN and NI groups were no significant difference(P>0.05);the abdominal fat rate in II group was significantly higher than that in NI group(P<0.05).3) Compared with the NN group,the values of redness(a*) and yellowness(b*) of breast muscle and leg muscle in II,IN and NI groups were no significant difference(P>0.05),the breast muscle brightness(L*) value in II group was significantly decreased(P<0.05);the pH45 min and pH24 h of breast muscle and leg muscle in IN and NI groups were significantly increased(P<0.05);the pH24 h of breast muscle in IN and NI groups was significantly increased(P<0.05);the pH24 h of leg muscle in II,IN and NI groups was significantly increased(P<0.05).4) Compared with the NN group,the relative expression level of glutamine phosphoribosylpyrophosphate amidotransferase(GPAT) gene of breast muscle and leg muscle in II,IN and NI groups at 21 and 42 days of age was no significant difference(P>0.05),the relative expression level of adenylosuccinate lyase(ADSL) gene of breast muscle in NI group and that of leg muscle in IN group at 21 days of age was significantly increased(P<0.05),the relative expression level of ADSL gene of breast muscle in II and IN groups at 42 days of age was significantly increased(P<0.05).These results suggest that adding exogenous inosinic acid in diets of breeding hens and offspring broilers have no significant effect on growth performance and slaughter performance of offspring broilers;and can increase muscle pH,decrease breast muscle L* value,improve the relative expression of ADSL gene,and improve meat quality of offspring broilers to a certain extent.
引文
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[9]闫俊书,宦海琳,周维仁,等.外源肌苷酸对肉鸡生长性能、肉品质及血清生化指标的影响[J].动物营养学报,2016,28(1):125-134.
[10]闫俊书,周维仁,张惠,等.饲粮肌苷酸对雪山草鸡胴体品质、肉质性状及血清中生化指标的影响[J].江苏农业学报,2012,28(6):1378-1385.
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