福建省福清地区马铃薯病毒病病原的分子检测
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摘要
2017年调查福建福清地区马铃薯病毒病的发生情况,以明确该地区马铃薯主要病毒病原。共采集了46份疑似感染病毒的马铃薯植株,提取总RNA,利用RT-PCR技术进行分子检测,结果表明,福清地区危害马铃薯的病毒有马铃薯Y病毒Potato virus Y(PVY)、马铃薯卷叶病毒Potato leaf roll virus(PLRV)、马铃薯S病毒Potato virus S(PVS),检出率分别为56.52%、17.39%和10.87%,以PVY检出率最高,说明PVY是危害该地区马铃薯样品的主要病毒病原。通过病毒复合侵染进行分析,发现该地区存在病毒复合侵染马铃薯现象。研究结果可为福清地区马铃薯种薯的引进和病毒病害防治提供参考依据。
The aim of this study is to investigate the occurrence of potato viruses in Fuqing area, Fujian province, and to diagnose the main viruses causing major diseases. In 2017, 46 infected potato plant samples were collected from Fuqing area, and the total RNA was extracted from the infected potato leaves. From these samples, PVY, PLRV and PVS were detected via the conventional RT-PCR techniques, and the detection rates were 56.52%, 17.39% and 10.87%, respectively, while other viruses were not detected. The analysis of complex infection showed that complex infection existed in potato plants in this area. This study can provide reference for the introduction of seed potato and the management strategy of virus diseases control in Fuqing area.
The aim of this study is to investigate the occurrence of potato viruses in Fuqing area, Fujian province, and to diagnose the main viruses causing major diseases. In 2017, 46 infected potato plant samples were collected from Fuqing area, and the total RNA was extracted from the infected potato leaves. From these samples, PVY, PLRV and PVS were detected via the conventional RT-PCR techniques, and the detection rates were 56.52%, 17.39% and 10.87%, respectively, while other viruses were not detected. The analysis of complex infection showed that complex infection existed in potato plants in this area. This study can provide reference for the introduction of seed potato and the management strategy of virus diseases control in Fuqing area.
引文
[1] SALAZAR L F.马铃薯病毒及其防治[M].阎文昭,张勇飞,译.北京:中国农业科学技术出版社,2000.
[2] 张威,白艳菊,高艳玲,等.马铃薯主产区病毒病发生情况调查[J].黑龙江农业科学,2010(4):71-73.
[3] 吴丽萍,王蒂,司怀军,等.马铃薯S病毒的RT-PCR检测[J].中国马铃薯,2006,20(4):200-203.
[4] WANG Biao,MA Yanli,ZHANG Zhibo,et al.Potato viruses in China [J].Crop Protection,2011,30:1117-1123.
[5] SOLOMON-BLACKBURN R M,BARKER H.Breeding virus resistant potatoes (Solanum tuberosum):a review of traditional and molecular approaches [J].Heredity,2001,86:17-35.
[6] 钟婷婷,蒲志刚,何俊蓉,等.四川省马铃薯主产区最新病毒病普查及血清学鉴定[J].西南农业学报,2008,21(1):96-99.
[7] 白艳菊,文景芝,杨明秀,等.西南地区与东北地区马铃薯主要病毒发生比较[J].东北农业大学学报,2007,38(6):733-736.
[8] 陈士华,刘晓磊,张晓婷,等.中国部分马铃薯产区马铃薯Y病毒(PVY)的株系分化与鉴定[J].河南农业大学学报,2011,45(5):548-551.
[9] 冯光惠,杜虎平,李夏隆,等.陕北地区马铃薯Y病毒的RT-PCR检测及其序列分析[J].山西农业科学,2014,42(9):941-944.
[10] 高芳銮,沈建国,史凤阳,等.中国马铃薯Y病毒的检测鉴定及CP基因的分子变异[J].中国农业科学,2013,46(15):3125-3133.
[11] 冯光惠,杜虎平,李夏隆,等.陕北地区马铃薯卷叶病毒的RT-PCR检测与序列分析[J].湖北农业科学,2014,53(19):4734-4736.
[12] DIENER T O.Potato spindle tuber “virus”.IV.A replicating,low molecular weight RNA [J].Virology,1971,45(2):411-428.
[13] 吴兴泉,陈士华,谢联辉.马铃薯X病毒的分子鉴定与检测技术[J].河南农业科学,2006(2):72-75.
[14] 杨雪芹,向本春,施磊.马铃薯脱毒及脱毒苗检测技术的研究进展[J].安徽农学通报,2007,13(8):98-100.
[15] 聂峰杰,詹红,张丽,等.RT-PCR技术对宁夏马铃薯脱毒种薯病毒检测的研究[J].植物保护,2016,42(5):188-193.
[16] KUMAR R,JEEVALATHA A,BASWARAJ R,et al.A multiplex RT-PCR assay for simultaneous detection of five viruses in potato [J].Journal of Plant Pathology,2017,99(1):37-45.
[17] 沈林林,邹文超,高芳銮,等.采用多基因联合方法鉴定福建长乐和福清产区马铃薯Y病毒株系组成[J].中国农业科学,2016,49(20):3918-3926.
[18] 张威,白艳菊,范国权,等.应用三重RT-PCR技术检测三种马铃薯病毒[J].中国马铃薯,2015(3):162-166.
[19] KOENIG R,ZIEBELL H.Sequence-modified primers for the differential RT-PCR detection of Andean potato latent and Andean potato mild mosaic viruses in quarantine tests[J].Archives of Virology,2014,159(5):1169-1173.
[20] AQSIQ.SN/T 1135.7-2009马铃薯A病毒检疫鉴定方法[S].2009-09-02.
[21] XU Huimin,D’AUBIN J,NIE J.Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes [J].Virology Journal,2010,7(1):25.
[22] AQSIQ.SN/T 1135.10-2013马铃薯V病毒检疫鉴定方法[S].2013-08-30.
[23] 文朝慧,南志标.甘肃省张掖地区苜蓿花叶病病原的检测[J].草业学报,2015,24(4):121-126.
[24] RIGA E,LARSEN R,EASTWELL K,et al.Rapid detection of tobacco rattle tobravirus in viruliferous Paratrichodorus allius from greenhouse and field specimens [J].Journal of Nematology,2009,41(1):60-63.
[25] 李学湛,吕典秋,白艳菊,等.应用NASH方法检测马铃薯类病毒(PSTVd)[J].中国马铃薯,2001(2):87-88.
[2] 张威,白艳菊,高艳玲,等.马铃薯主产区病毒病发生情况调查[J].黑龙江农业科学,2010(4):71-73.
[3] 吴丽萍,王蒂,司怀军,等.马铃薯S病毒的RT-PCR检测[J].中国马铃薯,2006,20(4):200-203.
[4] WANG Biao,MA Yanli,ZHANG Zhibo,et al.Potato viruses in China [J].Crop Protection,2011,30:1117-1123.
[5] SOLOMON-BLACKBURN R M,BARKER H.Breeding virus resistant potatoes (Solanum tuberosum):a review of traditional and molecular approaches [J].Heredity,2001,86:17-35.
[6] 钟婷婷,蒲志刚,何俊蓉,等.四川省马铃薯主产区最新病毒病普查及血清学鉴定[J].西南农业学报,2008,21(1):96-99.
[7] 白艳菊,文景芝,杨明秀,等.西南地区与东北地区马铃薯主要病毒发生比较[J].东北农业大学学报,2007,38(6):733-736.
[8] 陈士华,刘晓磊,张晓婷,等.中国部分马铃薯产区马铃薯Y病毒(PVY)的株系分化与鉴定[J].河南农业大学学报,2011,45(5):548-551.
[9] 冯光惠,杜虎平,李夏隆,等.陕北地区马铃薯Y病毒的RT-PCR检测及其序列分析[J].山西农业科学,2014,42(9):941-944.
[10] 高芳銮,沈建国,史凤阳,等.中国马铃薯Y病毒的检测鉴定及CP基因的分子变异[J].中国农业科学,2013,46(15):3125-3133.
[11] 冯光惠,杜虎平,李夏隆,等.陕北地区马铃薯卷叶病毒的RT-PCR检测与序列分析[J].湖北农业科学,2014,53(19):4734-4736.
[12] DIENER T O.Potato spindle tuber “virus”.IV.A replicating,low molecular weight RNA [J].Virology,1971,45(2):411-428.
[13] 吴兴泉,陈士华,谢联辉.马铃薯X病毒的分子鉴定与检测技术[J].河南农业科学,2006(2):72-75.
[14] 杨雪芹,向本春,施磊.马铃薯脱毒及脱毒苗检测技术的研究进展[J].安徽农学通报,2007,13(8):98-100.
[15] 聂峰杰,詹红,张丽,等.RT-PCR技术对宁夏马铃薯脱毒种薯病毒检测的研究[J].植物保护,2016,42(5):188-193.
[16] KUMAR R,JEEVALATHA A,BASWARAJ R,et al.A multiplex RT-PCR assay for simultaneous detection of five viruses in potato [J].Journal of Plant Pathology,2017,99(1):37-45.
[17] 沈林林,邹文超,高芳銮,等.采用多基因联合方法鉴定福建长乐和福清产区马铃薯Y病毒株系组成[J].中国农业科学,2016,49(20):3918-3926.
[18] 张威,白艳菊,范国权,等.应用三重RT-PCR技术检测三种马铃薯病毒[J].中国马铃薯,2015(3):162-166.
[19] KOENIG R,ZIEBELL H.Sequence-modified primers for the differential RT-PCR detection of Andean potato latent and Andean potato mild mosaic viruses in quarantine tests[J].Archives of Virology,2014,159(5):1169-1173.
[20] AQSIQ.SN/T 1135.7-2009马铃薯A病毒检疫鉴定方法[S].2009-09-02.
[21] XU Huimin,D’AUBIN J,NIE J.Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes [J].Virology Journal,2010,7(1):25.
[22] AQSIQ.SN/T 1135.10-2013马铃薯V病毒检疫鉴定方法[S].2013-08-30.
[23] 文朝慧,南志标.甘肃省张掖地区苜蓿花叶病病原的检测[J].草业学报,2015,24(4):121-126.
[24] RIGA E,LARSEN R,EASTWELL K,et al.Rapid detection of tobacco rattle tobravirus in viruliferous Paratrichodorus allius from greenhouse and field specimens [J].Journal of Nematology,2009,41(1):60-63.
[25] 李学湛,吕典秋,白艳菊,等.应用NASH方法检测马铃薯类病毒(PSTVd)[J].中国马铃薯,2001(2):87-88.