胶体金快速检测条法测定玉米油中玉米赤霉烯酮含量
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摘要
目的建立胶体金快速检测条法测定玉米油中玉米赤霉烯酮含量的分析方法,并与高效液相色谱法作比对。方法分别运用胶体金快速检测条法和免疫亲和层析净化高效液相色谱检测法对14个玉米油中玉米赤霉烯酮的含量进行检测。结果对实验结果进行成对样本t-检验,发现2种实验方法所得出的结果在95%的置信水平上无显著差异。同时运用胶体金快速检测条法对每个样品进行6次重复实验,分析其变异系数范围在2.9%～6.3%,说明此种方法的重复性较好。结论胶体金检测条法可以作为玉米油中玉米赤霉烯酮的快速检测方法。
Objective To establish a method for the determination of zearalenone in corn oil by colloidal gold strip method, and to compare with high performance liquid chromatography(HPLC). Methods The zearalenone content in 14 corn oil was detected by the methods of colloidal gold test strip and immunoaffinity chromatography purification HPLC, respectively. Results The results of the paired sample t-test showed that there was no significant difference between the 2 methods in the 95% confidence level. At the same time, the colloidal gold test strip was used to conduct 6 repeated experiments for each sample, the coefficient of variation were 2.9%-6.3%, indicating that the method has good repeatability. Conclusion The colloidal gold test strip can be used as a rapid method for the detection of zearalenone in corn oil.
Objective To establish a method for the determination of zearalenone in corn oil by colloidal gold strip method, and to compare with high performance liquid chromatography(HPLC). Methods The zearalenone content in 14 corn oil was detected by the methods of colloidal gold test strip and immunoaffinity chromatography purification HPLC, respectively. Results The results of the paired sample t-test showed that there was no significant difference between the 2 methods in the 95% confidence level. At the same time, the colloidal gold test strip was used to conduct 6 repeated experiments for each sample, the coefficient of variation were 2.9%-6.3%, indicating that the method has good repeatability. Conclusion The colloidal gold test strip can be used as a rapid method for the detection of zearalenone in corn oil.
引文
[1]杨小丽,杨美华,欧阳臻.玉米赤霉烯酮及其衍生物分析方法的研究进展[J].贵州农业科学,2011,39(7):234-238.Yang XL,Yang MH,Ouyang Z.Advances in the analytical methods of zearalenone and its derivatives[J].Guizhou Agric Sci,2011,39(7):234-238.
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[8]徐飞,刘峰,张亚军,等.液相色谱-串联质谱法测定粮食中的玉米赤霉烯酮[J].中国食品卫生杂志,2015,27(2):124-126.Xu F,Liu F,Zhang YJ,et al.Determination of zearalenone in grain and its products by liquid chromatography coupled with electrospray tandem mass spectrometry[J].Chin J Food Hyg,2015,27(2):124-126.
[9]应永飞,朱聪英,韦敏珏,等.液相色谱-串联质谱法测定饲料中14种霉菌毒素及其类似物[J].分析化学,2010,38(12):1759-1764.Ying YF,Zhu CY,Wei MY,et al.Simultaneous determination of 14mycotoxins and analogues in feeds by liquid chromatography-tandem mass spectrometry[J].Chin J Anal Chem,2010,38(12):1759-1764.
[10]宫小明,郝莹,董静,等.真菌毒素检测技术的研究进展[J].检验检疫学刊,2010,20(2):67-69.Gong XM,Hao Y,Dong J,et al.Review of research progress for the detection of mycotoxins[J].Inspect Quarant Sci,2010,20(2):67-69.
[11]包懿,李智瑾.谷物中玉米赤霉烯酮检测方法的研究进展[J].分析化学进展,2016,6(1):7-13.Bao Y,Li ZJ.Research progress on the determination methods of zearalenone in grain[J].Adv Anal Chem,2016,6(1):7-13.
[12]王桂芳,李培真,曹阳,等.胶体金测试条法对粮食中玉米赤霉烯酮快速定量测定的应用研究[J].粮食科技与经济,2014,39(1):32-34.Wang GF,Li PZ,Cao Y,et al.Application of colloidal gold test strip method for rapid quantitative determination of zearalenone in corn[J].Food Sci Technol Econom,2014,39(1):32-34.
[13]牟钧,潘蓓,杨军,等.胶体金免疫层析法快速测定玉米和小麦中玉米赤霉烯酮[J].粮油食品科技,2011,19(5):43-45.Mou J,Pan B,Yang J,et al.Rapid determination of zearalenone in corn and wheat by colloidal gold immunochromatography[J].Sci Technol Cere Oils Foods,2011,19(5):43-45.
[14]王丽丽.假设检验中关于检验问题探讨[J].科技视界,2018,(22):82-84.Wang LL.Discussion on T test problems in hypothesis test[J].Sci Technol Vision,2018,(22):82-84.
[15]兰冲锋.统计学教学中关于假设检验问题探讨[J].湖南城市学院学报(自然科学版),2016,25(2):303-304.Lan CF.Discussion on hypothesis test problems in statistics teaching[J].JHunan City Univ(Nat Sci Ed),2016,25(2):303-304.
[2]姜淑贞,杨维仁,杨在宾.玉米赤霉烯酮的代谢、毒性及其预防措施[J].动物营养学报,2011,23(2):196-202.Jiang SZ,Yang WR,Yang ZB.Metabolm,toxicity and preventive measures of zearalenone[J].Chin J Anim Nutr,2011,23(2):196-202.
[3]何庆华,许杨.玉米赤霉烯酮毒性研究及检测方法进展[J].卫生研究,2005,34(4):502-504.He QH,Xu Y.Advance in study on zearalenone’s toxicity and determination[J].J Hyg Res,2005,34(4):502-504.
[4]单妹,许梓荣,冯建蕾.玉米赤霉烯酮对家畜繁殖性能和人体健康的影响[J].中国畜牧兽医,2006,33(1):3-5.Shan M,Xu ZR,Feng JL.Influence of zearalenon on livestocks reproductive performance and human health[J].Chin Anim Husband Veter Med,2006,33(1):3-5.
[5]吴文达,王宝杰,蔡兰芬,等.薄层色谱和高效液相色谱联合检测玉米赤霉烯酮的方法研究[J].畜牧与兽医,2010,42(7):17-20.Wu WD,Wang BJ,Cai LF,et al.Detection of zearalenone by thin layer chromatography combined with high performance liquid chromatography[J].Anim Husband Veter Med,2010,42(7):17-20.
[6]康维钧,王玉平,杨福江,等.反相高效液相色谱法测定玉米中玉米赤霉烯酮[J].分析实验室,2007,26(10):66-68.Kang WJ,Wang YP,Yang FJ,et al.Detection of zearalenone in corn by high performance liquid chromatography with fluorescence detection[J].Chin J Anal Lab,2007,26(10):66-68.
[7]谢刚,王松雪,崔华,等.超高效液相色谱法快速检测粮食中玉米赤霉烯酮的含量[J].粮油食品科技,2014,22(2):71-75.Xie G,Wang SX,Cui H,et al.Rapid detection of zearalenone in grain by UPLC[J].Sci Technol Cere Oils Foods,2014,22(2):71-75.
[8]徐飞,刘峰,张亚军,等.液相色谱-串联质谱法测定粮食中的玉米赤霉烯酮[J].中国食品卫生杂志,2015,27(2):124-126.Xu F,Liu F,Zhang YJ,et al.Determination of zearalenone in grain and its products by liquid chromatography coupled with electrospray tandem mass spectrometry[J].Chin J Food Hyg,2015,27(2):124-126.
[9]应永飞,朱聪英,韦敏珏,等.液相色谱-串联质谱法测定饲料中14种霉菌毒素及其类似物[J].分析化学,2010,38(12):1759-1764.Ying YF,Zhu CY,Wei MY,et al.Simultaneous determination of 14mycotoxins and analogues in feeds by liquid chromatography-tandem mass spectrometry[J].Chin J Anal Chem,2010,38(12):1759-1764.
[10]宫小明,郝莹,董静,等.真菌毒素检测技术的研究进展[J].检验检疫学刊,2010,20(2):67-69.Gong XM,Hao Y,Dong J,et al.Review of research progress for the detection of mycotoxins[J].Inspect Quarant Sci,2010,20(2):67-69.
[11]包懿,李智瑾.谷物中玉米赤霉烯酮检测方法的研究进展[J].分析化学进展,2016,6(1):7-13.Bao Y,Li ZJ.Research progress on the determination methods of zearalenone in grain[J].Adv Anal Chem,2016,6(1):7-13.
[12]王桂芳,李培真,曹阳,等.胶体金测试条法对粮食中玉米赤霉烯酮快速定量测定的应用研究[J].粮食科技与经济,2014,39(1):32-34.Wang GF,Li PZ,Cao Y,et al.Application of colloidal gold test strip method for rapid quantitative determination of zearalenone in corn[J].Food Sci Technol Econom,2014,39(1):32-34.
[13]牟钧,潘蓓,杨军,等.胶体金免疫层析法快速测定玉米和小麦中玉米赤霉烯酮[J].粮油食品科技,2011,19(5):43-45.Mou J,Pan B,Yang J,et al.Rapid determination of zearalenone in corn and wheat by colloidal gold immunochromatography[J].Sci Technol Cere Oils Foods,2011,19(5):43-45.
[14]王丽丽.假设检验中关于检验问题探讨[J].科技视界,2018,(22):82-84.Wang LL.Discussion on T test problems in hypothesis test[J].Sci Technol Vision,2018,(22):82-84.
[15]兰冲锋.统计学教学中关于假设检验问题探讨[J].湖南城市学院学报(自然科学版),2016,25(2):303-304.Lan CF.Discussion on hypothesis test problems in statistics teaching[J].JHunan City Univ(Nat Sci Ed),2016,25(2):303-304.