取食香根草后水稻螟虫对杀虫剂敏感度变化
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摘要
【目的】香根草能有效诱集水稻螟虫产卵,且孵化的幼虫取食香根草一定时间后死亡,可用于水稻螟虫的田间防控。但田间防治往往是多种防治措施相结合。为了研究取食香根草后的幼虫对常用杀虫剂的敏感度,【方法】利用稻苗浸渍法测定了茚虫威、氯虫苯甲酰胺、多杀菌素、甲氨基阿维菌素、氟虫胺、氰氟虫腙、阿维菌素、毒死蜱8种常用杀虫剂对水稻大螟和二化螟的毒力作用,以及取食香根草后螟虫对这些药剂的敏感度变化。【结果】8种杀虫剂(氰氟虫腙,甲氨基阿维菌素,氯虫苯甲酰胺,氟虫胺,多杀菌素,阿维菌素,茚虫威,毒死蜱)对大螟的LC_(50)依次为1.04,1.40,2.67,3.48,7.59,13.03,16.57,23.28 mg/L;对二化螟的LC_(50)依次为1.94,2.07,4.20,8.93,9.72,17.63,9.51,64.68mg/L。与取食水稻的螟虫比较,香根草饲喂3d后,大螟幼虫对8种药剂的敏感性均有所提高,致死率提高13.3～22.2个百分点,其中,对氯虫苯甲酰胺、多杀菌素、氟虫胺、氰氟虫腙、毒死蜱的敏感度显著提高;而香根草饲喂后的二化螟幼虫对所有测定药剂敏感度均显著提高,致死率提高26.7～33.3个百分点。二化螟幼虫对所有测定药剂敏感度升高更为明显。【结论】初步明确了水稻螟虫取食香根草后对杀虫剂的敏感度有不同程度提高。
【Objective】Previous studies showed that vetiver grass(Vetiveria zizanioides) can effectively attract female adults of Sesamia inferens(Walker) and Chilo suppressalis(Walker) to lay eggs, while hatched larvae will die after feeding on vetiver grass for a certain time. Therefore, vetiver grass is recommended for field control of rice stem borers.However, the combination of various measures is always used for field control. At present, chemical control is still an irreplaceable measure. Nevertheless, the sensitivity change of rice stem borers after feeding on vetiver to commonly used insecticides is still unclear. 【Method】Seedling dipping bioassay method was used to investigate the toxicity of eight insecticides, including indoxacarb, chlorantraniliprole, spinosad, emamectin benzoate, sulfluramid, metaflumizone,abamectin and chlopyrifos to both S. inferens and C. suppressalis, and the sensitivity changes of these two insect species to these insecticides after feeding on vetiver grass. 【Result】 LC_(50) to S. inferens were as follows: metaflumizone 1.04 mg/L, emamectin benzoate 1.40 mg/L, chlorantraniliprole 2.67 mg/L, sulfluramid 3.48 mg/L, spinosad 7.59 mg/L,abamectin 13.03 mg/L, indoxacarb 16.57 mg/L, chlorpyrifos 23.28 mg/L; and to C. suppressalis were metaflumizone1.94 mg/L, emamectin benzoate 2.07 mg/L, chlorantraniliprole 4.20 mg/L, sulfluramid 8.93 mg/L, indoxacarb 9.51 mg/L,spinosad 9.72 mg/L, abamectin 17.63 mg/L, chlorpyrifos 64.68 mg/L. The sensitivity of the two kinds of rice stem borers fed on vetvier grass was increased as compared with the stem borers fed on rice. Three days after feeding on vetiver grass,the sensitivity of S. inferens larvae to eight pesticides was improved, and the mortality was also increased by13.3%-22.2%. The sensitivities of S. inferens to chlorantraniliprole, spinosad, sulfluramid, metaflumizone and chlorpyrifos were significantly increased, while the sensitivity of C. suppressalis larvae to all the tested pesticides was significantly increased after feeding on vetiver grass with an increased mortality by 26.7%-33.3%. Overall, the sensitivity of C. suppressalis larvae was obviously increased more than that of S. inferens. 【Conclusion】The sensitivity of rice stem borers to eight commonly used insecticides was dramatically increased after feeding on vetiver grass.
【Objective】Previous studies showed that vetiver grass(Vetiveria zizanioides) can effectively attract female adults of Sesamia inferens(Walker) and Chilo suppressalis(Walker) to lay eggs, while hatched larvae will die after feeding on vetiver grass for a certain time. Therefore, vetiver grass is recommended for field control of rice stem borers.However, the combination of various measures is always used for field control. At present, chemical control is still an irreplaceable measure. Nevertheless, the sensitivity change of rice stem borers after feeding on vetiver to commonly used insecticides is still unclear. 【Method】Seedling dipping bioassay method was used to investigate the toxicity of eight insecticides, including indoxacarb, chlorantraniliprole, spinosad, emamectin benzoate, sulfluramid, metaflumizone,abamectin and chlopyrifos to both S. inferens and C. suppressalis, and the sensitivity changes of these two insect species to these insecticides after feeding on vetiver grass. 【Result】 LC_(50) to S. inferens were as follows: metaflumizone 1.04 mg/L, emamectin benzoate 1.40 mg/L, chlorantraniliprole 2.67 mg/L, sulfluramid 3.48 mg/L, spinosad 7.59 mg/L,abamectin 13.03 mg/L, indoxacarb 16.57 mg/L, chlorpyrifos 23.28 mg/L; and to C. suppressalis were metaflumizone1.94 mg/L, emamectin benzoate 2.07 mg/L, chlorantraniliprole 4.20 mg/L, sulfluramid 8.93 mg/L, indoxacarb 9.51 mg/L,spinosad 9.72 mg/L, abamectin 17.63 mg/L, chlorpyrifos 64.68 mg/L. The sensitivity of the two kinds of rice stem borers fed on vetvier grass was increased as compared with the stem borers fed on rice. Three days after feeding on vetiver grass,the sensitivity of S. inferens larvae to eight pesticides was improved, and the mortality was also increased by13.3%-22.2%. The sensitivities of S. inferens to chlorantraniliprole, spinosad, sulfluramid, metaflumizone and chlorpyrifos were significantly increased, while the sensitivity of C. suppressalis larvae to all the tested pesticides was significantly increased after feeding on vetiver grass with an increased mortality by 26.7%-33.3%. Overall, the sensitivity of C. suppressalis larvae was obviously increased more than that of S. inferens. 【Conclusion】The sensitivity of rice stem borers to eight commonly used insecticides was dramatically increased after feeding on vetiver grass.
引文
[1]蒋学辉,章强华,胡仕孟,谢士杰,徐喜刚.浙江省水稻二化螟抗药性现状与治理对策.植保技术与推广,2001,21(3):27-29.Jiang X H,Zhang Q H,Hu S M,Xie S J,Xu X G.The status of pesticide resistance of rice stalk borer in Zhejiang Province and their management tactics.Plant Prot Technol Ext,2001,21(3):27-29.(in Chinese with English abstract)
[2]张真真.二化螟抗药性监测及其对氯虫苯甲酰胺敏感基线的建立.南京:南京农业大学,2012.Monitoring of insecticide resistance and baseline susceptibility of Chilo suppressalis(Lepidoptera:Pyralidae)to chlorantraniliprole.Nanjing:Nanjing Agricultural University,2012.(in Chinese with English abstract)
[3]彭少兵.转型时期杂交水稻的困境与出路.作物学报,2016,42(3):313-319.Peng S B.Dilemma and way-out of hybrid rice during the transition period in China.Acta Agron Sin,2016,42(3):313-319.(in Chinese with English abstract)
[4]姜卫华.二化螟的抗药性及综合防治研究.南京:南京农业大学,2011.Jiang W H.Study on insecticide resistance and integrated control of rice stem borer,Chilo suppressalis.Nanjing:Nanjing Agricultural University,2011.(in Chinese with English abstract)
[5]张扬,王保菊,韩平,韩召军.二化螟抗药性检测方法比较和抗药性监测.南京农业大学学报,2014,37(6):37-43.Zhang Y,Wang B J,Han P,Han Z J.Comparison of methods for testing insecticide resistance in Chilo suppressalis and the resistance monitored.J Nanjing Agricul Univ,2014,37(6):37-43.(in Chinese with English abstract)
[6]张海艳,李海东,韩召军.大螟田间种群对不同杀虫剂敏感性的差异.中国稻米,2012,18(1):29-33.Zhang H Y,Li H D,Han Z J.Sensitivity of field populations to different insecticides in rice stem borer,Sesamia inferens(Walker).China Rice,2012,18(1):29-33.(in Chinese with English abstract)
[7]胡君,陈文明,张真真,郑雪松,靳建超,苏建亚,高聪芬,沈晋良.长江流域稻区二化螟抗药性监测.中国水稻科学,2010,24(5):509-515.Hu J,Chen W M,Zhang Z Z,Zheng X S,Jin J C,Su J Y,Gao C F,Shen J L.Insecticide resistance monitoring of Chilo suppressalis in the drainage area of the Yangtze River China.Chin J Rice Sci,2010,24(5):509-515.(in Chinese with English abstract)
[8]梁齐,鲁艳辉,何晓婵,郑许松,徐红星,杨亚军,田俊策,吕仲贤.诱集植物在害虫治理中的最新研究进展.生物安全学报,2015,24(3):184-193.Liang Q,Lu Y H,He X C,Zheng X S,Xu H X,Yang Y J,Tian J C,Lu Z X.Mini review of the significance of trap crop in insect pest management.J Biosafety,2015,24:184-193.(in Chinese with English abstract)
[9]陈先茂,彭春瑞,姚锋先,关贤交,王华伶,邓国强.利用香根草诱集诱杀水稻螟虫的技术及效果研究.江西农业学报,2007,19(12):51-52.Chen X M,Peng C R,Yao F X,Guan X J,Wang H L,Deng G Q.Study on technique and effect of vetiver for trapping and killing rice borer.Acta Agric Jiangxi,2007,19:51-52(in Chinese with English astract).
[10]郑许松,徐红星,陈桂华,吴降星,吕仲贤.苏丹草和香根草作为诱虫植物对稻田二化螟种群的抑制作用评估.中国生物防治学报,2009,25(4):299-303.Zheng X S,Xu H X,Chen G H,Wu J X,Lu Z X.Potential function of Sudan grass and vetiver grass as trap crops for suppressing population of stripped stem borer,Chilo suppressalis in rice.Chin J Biol Control,2009,04:299-303.(in Chinese with English abstract)
[11]鲁艳辉,高广春,郑许松,吕仲贤.诱集植物香根草对二化螟幼虫致死的作用机制.中国农业科学,2017,50(3):486-495.Lu Y H,Gao G C,Zheng X S,Lu Z X.The lethal mechanism of trap plant vetiver grass(Vetiveria zizanioides)against the larvae of striped stem borer,Chilo suppressalis.Sci Agric Sin,2017,50(3):486-495.(in Chinese with English abstract)
[12]鲁艳辉,梁齐,郑许松,吕仲贤.诱集植物香根草对大螟幼虫营养作用及消化酶的影响.中国生物防治学报,2017(5):719-724.Lu Y H,Liang Q,Zheng X S,Lu Z X.Effects of trap plant vetiver grass(Vetiveria zizanioides)on nutritional and digestive enzyme activities of pink stemborer(Sesamia inferens)larvae.Chin J Biol Control,2017(5):719-724.(in Chinese with English abstract)
[13]戴长庚,李凯龙,王立峰,谭显胜,胡阳,袁哲明,傅强.基于均匀设计优化的大螟实用饲料配方及继代饲养.中国水稻科学,2013,27(4):434-439.Dai C G,Li K L,Wang L F,Tan X S,Hu Y,Yuan Z M,Fu Q.An oligidic diet for Sesamia inferens optimized by uniform designand successive rearing.Chin J Rice Sci,2013,27(4):434-439.(in Chinese with English abstract)
[14]胡阳,郑永利,曹国连,傅强.利用半人工饲料大规模简便化饲养二化螟.中国水稻科学,2013,27(5):535-538.Hu Y,Zheng Y L,Cao G L,Fu Q,2013.A technique for rearing Chilo suppressalis in the large scale with an oligidic diet in laboratory.Chin J Rice Sci,2013,27(5):535-538.(in Chinese with English abstract)
[15]赵钧,付文曦,韩召军.大螟对7种杀虫剂的抗药性监测及相对敏感基线验证.南京农业大学学报,2016,39(1):84-88.Zhao J,Fu W X,Han Z J.Resistance monitoring of Sesamia inferens(Walker)to seven insecticides and verification of related baseline data.J Nanjing Agric Univ,2016,39(1):84-88.
[16]Su J Y,Zhang Z Z,Wu M,Gao C F.Geographic susceptibility of Chilo suppressalis Walker(Lepidoptera:Crambidae),to chlorantraniliprole in China.Pest Manag Sci,2014,70(6):989-995.
[17]Lu Y H,Wang G R,Zhong L Q,Zhang F C,Bai Q,Zheng X S,Lu Z X.Resistance monitoring of Chilo suppressalis(Walker)(Lepidoptera:Crambidae)to chlorantraniliprole in eight field populations from east and central China.Crop Prot,2017,100:196-202.
[18]周阳,赵中华,杨普云.以绿色防控促进生态文明建设.中国植保导刊,2013,33(11):75-78.Zhou Y,Zhao Z H,Yang P Y.Promoting the construction of ecological civilization with green prevention and control.China Plant Prot,2013,33(11):75-78.(in Chinese with English abstract)
[19]覃春华,匡晶,余明华,李建洪.不同水生蔬菜对斜纹夜蛾药剂敏感性的影响.中国蔬菜,2011,8:71-75.Qin C H,Kuang J,Yu M H,Li J H.Effects of feeding different aquatic vegetables on sensitivity to insecticides of Spodoptera litura(Lepidoptera:Noctuidae).China Veg,2011,8:71-75.(in Chinese with English abstract)
[20]乐伟,马伟华,王沫.取食不同寄主植物的二个甜菜夜蛾品系对药剂的敏感性.昆虫知识,2005,42(3):290-294.Le W,Ma W H,Wang M.The variation of sensitivity to insecticides of Spodoptera exigua strains feeding on different host plants.Chin Bull Entomol,2005,42(3):290-294.(in Chinese with English abstract)
[21]吕朝军,韩巨才,刘慧平,任一新,马林.寄主植物对苹果黄蚜药剂敏感性及解毒酶活性的影响.植物保护学报,2007,34(5):534-538.LüC J,Han J C,Liu H P,Ren Y X,Ma L.Influence of host plants to detoxification enzymes and susceptibilities to insecticides on Aphis citricola von der Goot.Acta Phytophy Sin,2007,34(5):534-538.(in Chinese with English abstract)
[2]张真真.二化螟抗药性监测及其对氯虫苯甲酰胺敏感基线的建立.南京:南京农业大学,2012.Monitoring of insecticide resistance and baseline susceptibility of Chilo suppressalis(Lepidoptera:Pyralidae)to chlorantraniliprole.Nanjing:Nanjing Agricultural University,2012.(in Chinese with English abstract)
[3]彭少兵.转型时期杂交水稻的困境与出路.作物学报,2016,42(3):313-319.Peng S B.Dilemma and way-out of hybrid rice during the transition period in China.Acta Agron Sin,2016,42(3):313-319.(in Chinese with English abstract)
[4]姜卫华.二化螟的抗药性及综合防治研究.南京:南京农业大学,2011.Jiang W H.Study on insecticide resistance and integrated control of rice stem borer,Chilo suppressalis.Nanjing:Nanjing Agricultural University,2011.(in Chinese with English abstract)
[5]张扬,王保菊,韩平,韩召军.二化螟抗药性检测方法比较和抗药性监测.南京农业大学学报,2014,37(6):37-43.Zhang Y,Wang B J,Han P,Han Z J.Comparison of methods for testing insecticide resistance in Chilo suppressalis and the resistance monitored.J Nanjing Agricul Univ,2014,37(6):37-43.(in Chinese with English abstract)
[6]张海艳,李海东,韩召军.大螟田间种群对不同杀虫剂敏感性的差异.中国稻米,2012,18(1):29-33.Zhang H Y,Li H D,Han Z J.Sensitivity of field populations to different insecticides in rice stem borer,Sesamia inferens(Walker).China Rice,2012,18(1):29-33.(in Chinese with English abstract)
[7]胡君,陈文明,张真真,郑雪松,靳建超,苏建亚,高聪芬,沈晋良.长江流域稻区二化螟抗药性监测.中国水稻科学,2010,24(5):509-515.Hu J,Chen W M,Zhang Z Z,Zheng X S,Jin J C,Su J Y,Gao C F,Shen J L.Insecticide resistance monitoring of Chilo suppressalis in the drainage area of the Yangtze River China.Chin J Rice Sci,2010,24(5):509-515.(in Chinese with English abstract)
[8]梁齐,鲁艳辉,何晓婵,郑许松,徐红星,杨亚军,田俊策,吕仲贤.诱集植物在害虫治理中的最新研究进展.生物安全学报,2015,24(3):184-193.Liang Q,Lu Y H,He X C,Zheng X S,Xu H X,Yang Y J,Tian J C,Lu Z X.Mini review of the significance of trap crop in insect pest management.J Biosafety,2015,24:184-193.(in Chinese with English abstract)
[9]陈先茂,彭春瑞,姚锋先,关贤交,王华伶,邓国强.利用香根草诱集诱杀水稻螟虫的技术及效果研究.江西农业学报,2007,19(12):51-52.Chen X M,Peng C R,Yao F X,Guan X J,Wang H L,Deng G Q.Study on technique and effect of vetiver for trapping and killing rice borer.Acta Agric Jiangxi,2007,19:51-52(in Chinese with English astract).
[10]郑许松,徐红星,陈桂华,吴降星,吕仲贤.苏丹草和香根草作为诱虫植物对稻田二化螟种群的抑制作用评估.中国生物防治学报,2009,25(4):299-303.Zheng X S,Xu H X,Chen G H,Wu J X,Lu Z X.Potential function of Sudan grass and vetiver grass as trap crops for suppressing population of stripped stem borer,Chilo suppressalis in rice.Chin J Biol Control,2009,04:299-303.(in Chinese with English abstract)
[11]鲁艳辉,高广春,郑许松,吕仲贤.诱集植物香根草对二化螟幼虫致死的作用机制.中国农业科学,2017,50(3):486-495.Lu Y H,Gao G C,Zheng X S,Lu Z X.The lethal mechanism of trap plant vetiver grass(Vetiveria zizanioides)against the larvae of striped stem borer,Chilo suppressalis.Sci Agric Sin,2017,50(3):486-495.(in Chinese with English abstract)
[12]鲁艳辉,梁齐,郑许松,吕仲贤.诱集植物香根草对大螟幼虫营养作用及消化酶的影响.中国生物防治学报,2017(5):719-724.Lu Y H,Liang Q,Zheng X S,Lu Z X.Effects of trap plant vetiver grass(Vetiveria zizanioides)on nutritional and digestive enzyme activities of pink stemborer(Sesamia inferens)larvae.Chin J Biol Control,2017(5):719-724.(in Chinese with English abstract)
[13]戴长庚,李凯龙,王立峰,谭显胜,胡阳,袁哲明,傅强.基于均匀设计优化的大螟实用饲料配方及继代饲养.中国水稻科学,2013,27(4):434-439.Dai C G,Li K L,Wang L F,Tan X S,Hu Y,Yuan Z M,Fu Q.An oligidic diet for Sesamia inferens optimized by uniform designand successive rearing.Chin J Rice Sci,2013,27(4):434-439.(in Chinese with English abstract)
[14]胡阳,郑永利,曹国连,傅强.利用半人工饲料大规模简便化饲养二化螟.中国水稻科学,2013,27(5):535-538.Hu Y,Zheng Y L,Cao G L,Fu Q,2013.A technique for rearing Chilo suppressalis in the large scale with an oligidic diet in laboratory.Chin J Rice Sci,2013,27(5):535-538.(in Chinese with English abstract)
[15]赵钧,付文曦,韩召军.大螟对7种杀虫剂的抗药性监测及相对敏感基线验证.南京农业大学学报,2016,39(1):84-88.Zhao J,Fu W X,Han Z J.Resistance monitoring of Sesamia inferens(Walker)to seven insecticides and verification of related baseline data.J Nanjing Agric Univ,2016,39(1):84-88.
[16]Su J Y,Zhang Z Z,Wu M,Gao C F.Geographic susceptibility of Chilo suppressalis Walker(Lepidoptera:Crambidae),to chlorantraniliprole in China.Pest Manag Sci,2014,70(6):989-995.
[17]Lu Y H,Wang G R,Zhong L Q,Zhang F C,Bai Q,Zheng X S,Lu Z X.Resistance monitoring of Chilo suppressalis(Walker)(Lepidoptera:Crambidae)to chlorantraniliprole in eight field populations from east and central China.Crop Prot,2017,100:196-202.
[18]周阳,赵中华,杨普云.以绿色防控促进生态文明建设.中国植保导刊,2013,33(11):75-78.Zhou Y,Zhao Z H,Yang P Y.Promoting the construction of ecological civilization with green prevention and control.China Plant Prot,2013,33(11):75-78.(in Chinese with English abstract)
[19]覃春华,匡晶,余明华,李建洪.不同水生蔬菜对斜纹夜蛾药剂敏感性的影响.中国蔬菜,2011,8:71-75.Qin C H,Kuang J,Yu M H,Li J H.Effects of feeding different aquatic vegetables on sensitivity to insecticides of Spodoptera litura(Lepidoptera:Noctuidae).China Veg,2011,8:71-75.(in Chinese with English abstract)
[20]乐伟,马伟华,王沫.取食不同寄主植物的二个甜菜夜蛾品系对药剂的敏感性.昆虫知识,2005,42(3):290-294.Le W,Ma W H,Wang M.The variation of sensitivity to insecticides of Spodoptera exigua strains feeding on different host plants.Chin Bull Entomol,2005,42(3):290-294.(in Chinese with English abstract)
[21]吕朝军,韩巨才,刘慧平,任一新,马林.寄主植物对苹果黄蚜药剂敏感性及解毒酶活性的影响.植物保护学报,2007,34(5):534-538.LüC J,Han J C,Liu H P,Ren Y X,Ma L.Influence of host plants to detoxification enzymes and susceptibilities to insecticides on Aphis citricola von der Goot.Acta Phytophy Sin,2007,34(5):534-538.(in Chinese with English abstract)