不同壮秧剂对水稻幼苗生长及生理特性的影响
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摘要
为探究不同壮秧剂对水稻幼苗生长及生理特性的影响,以龙粳31和垦稻24为供试品种,共设置9种调节剂[多效唑(MET)、脱落酸(ABA)、壳寡糖(COS)、萘乙酸(NAA)、烯效唑(S3307)、吲哚丁酸钾(IBAK)、缩节胺(DPC)、1-(3-甲酸甲酯-1,2,4-三唑)-基-3.3-二甲基-2-丁酮(CGR3)和胺鲜酯(DTA-6)]与壮秧肥配施的新型壮秧剂处理,以不添加调节剂的壮秧肥为CK,研究不同壮秧剂对水稻幼苗形态建成、生物量积累、分配和生理特性等方面的调控效果。结果表明,MET、COS、ABA、NAA、IBAK、DPC和CGR3均能促进龙粳31和垦稻24叶片中光合色素含量,其中,MET和ABA处理的2个品种叶片叶绿素a、叶绿素b、叶黄素和总叶绿素均与CK间差异达到显著水平。MET、ABA和COS能有效促进同化产物向根中转运,2个品种根中淀粉含量均高于CK。各壮秧剂处理(除NAA和DPC外)均对龙粳31和垦稻24幼苗生长有促进作用,且其根冠比均显著高于CK。MET、S3307、DPC和DTA-6处理均提高了2个品种的壮苗指数和充实度。MET和S3307处理降低了2个品种的株高,但增加了茎基宽。COS和ABA处理下,龙粳31的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性均高于CK,MET处理下的龙粳31和垦稻24的CAT活性最高。综上可知,MET、ABA和COS这3种壮秧剂能够提高龙粳31和垦稻24秧苗素质和植株抗性,有利于水稻幼苗生长,其中垦稻24作用效果优于龙粳31。本研究结果为化控技术在壮秧剂中的应用和水稻培育壮秧提供了理论依据和技术指导。
In order to estimate the effects of different seedling strengthening agent on the growth and physiological characteristics of rice seedlings,rice varieties of Longjing 31 and Kendao Rice 24 were used in this experiment,and a total of 9 kinds of regulators were set up,such as paclobutrazol( MET),Abscisic acid( ABA),chitosan oligosaccharide( COS),1-naphthylacetic acid( NAA),unic-onazole( S3307),indole-3-butyricacid,potassium-salt( IBAK),mepiquatchloride( DPC),methyl-1-( 3,3-dimenthyl-2-oxobutyl)-1 H-1,2,4-triazole-3-carboxy-late( CGR3) and diethyl aminoethylhexanoate( DTA-6) " for treatments. CK is a strong seedling without regulators. The effect of different seedlings on the morphological construction,biomass accumulation,distribution and physiological characteristics of rice seedlings was studied. The results showed that MET,COS,ABA,NAA,IBAK,DPC and CGR3 treatments promoted the content of photosynthetic pigments in leaves of Longjing 31 and Kendao 24. Among them,treatment of MET and ABA significantly increased chlorophyll a,chlorophyll b,lutein and total chlorophyll contents in leaves of two cultivars.MET,ABA and COS effectively promoted the transport of assimilated products to roots,and the starch content in roots of the two varieties was higher than CK. All treatments( except NAA and DPC) promoted the growth of Longjing 31 and Kendao 24 seedlings,and their root-shoot ratio was significantly higher than CK. MET,S3307,DPC and DTA-6 treatments improved the seedling in-dex and plant height ratio of the two varieties. MET and S3307 treatments reduced the heights of Longjing 31 and Kengdao 24 and increased the width of stem base. The activities of superoxide dismutase( SOD),peroxidase( POD) and catalase( CAT) of Longjing 31 were higher than those of CK under COS and ABA treatments,while the CAT activity of two cultivars treated with MET reached the maximum of all treatment. In conclusion,MET,ABA and COS can improve the seedling quality and plant resistance of Longjing 31 and Kendao 24,which is beneficial to the growth of rice seedlings,the effect of Kendao 24 was better than Longjing 31 among them. The results of this study provide theoretical basis and technical guidance for the application of regulators in rice seedling strengthening agents and the cultivation of rice seedlings.
In order to estimate the effects of different seedling strengthening agent on the growth and physiological characteristics of rice seedlings,rice varieties of Longjing 31 and Kendao Rice 24 were used in this experiment,and a total of 9 kinds of regulators were set up,such as paclobutrazol( MET),Abscisic acid( ABA),chitosan oligosaccharide( COS),1-naphthylacetic acid( NAA),unic-onazole( S3307),indole-3-butyricacid,potassium-salt( IBAK),mepiquatchloride( DPC),methyl-1-( 3,3-dimenthyl-2-oxobutyl)-1 H-1,2,4-triazole-3-carboxy-late( CGR3) and diethyl aminoethylhexanoate( DTA-6) " for treatments. CK is a strong seedling without regulators. The effect of different seedlings on the morphological construction,biomass accumulation,distribution and physiological characteristics of rice seedlings was studied. The results showed that MET,COS,ABA,NAA,IBAK,DPC and CGR3 treatments promoted the content of photosynthetic pigments in leaves of Longjing 31 and Kendao 24. Among them,treatment of MET and ABA significantly increased chlorophyll a,chlorophyll b,lutein and total chlorophyll contents in leaves of two cultivars.MET,ABA and COS effectively promoted the transport of assimilated products to roots,and the starch content in roots of the two varieties was higher than CK. All treatments( except NAA and DPC) promoted the growth of Longjing 31 and Kendao 24 seedlings,and their root-shoot ratio was significantly higher than CK. MET,S3307,DPC and DTA-6 treatments improved the seedling in-dex and plant height ratio of the two varieties. MET and S3307 treatments reduced the heights of Longjing 31 and Kengdao 24 and increased the width of stem base. The activities of superoxide dismutase( SOD),peroxidase( POD) and catalase( CAT) of Longjing 31 were higher than those of CK under COS and ABA treatments,while the CAT activity of two cultivars treated with MET reached the maximum of all treatment. In conclusion,MET,ABA and COS can improve the seedling quality and plant resistance of Longjing 31 and Kendao 24,which is beneficial to the growth of rice seedlings,the effect of Kendao 24 was better than Longjing 31 among them. The results of this study provide theoretical basis and technical guidance for the application of regulators in rice seedling strengthening agents and the cultivation of rice seedlings.
引文
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[2]柳延涛,徐安阳,段维,王鹏,刘胜利.缩节胺、多效唑和矮壮素对向日葵生理特性的影响[J].中国油料作物学报,2018,40(2):241-246
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[6]赵树琪,张华崇,闫振华,李蔚,戴宝生,黄晓莉.缩节胺在我国棉花生产中应用研究概述[J].棉花科学,2018,40(3):7-11
[7]解文孝,韩勇,李建国,刘博,姜秀英,刘军,吕军,张宇.多效唑浸种对水稻幼苗生长的影响[J].安徽农学通报,2013(18):20-21
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[9]郑春芳,陈继浓,仇建标,刘伟成,张呈念,彭欣.烯效唑对低温胁迫下秋茄幼苗光合作用与抗氧化系统的影响[J].植物生理学报,2016,52(1):109-116
[10]杨文钰,项祖芬,任万君,王小春.烯效唑对水稻氮代谢及稻米蛋白质含量的影响[J].中国水稻科学,2005,19(1):63-67
[11]张晓明,郝保平,张鑫,张延芳,曹卫东,张海峰,曹建琴,郑普山.缩节胺对毛叶苕子生长及籽粒产量的影响[J].山西农业科学,2017,45(10):1627-1630
[12] Nikolaeva T N,Zagoskina N V,Zaprometov M N. Production of phenolic compounds in callus cultures of tea plant under the effect of2,4-D and NAA[J]. Russian Journal of Plant Physiology,2009,56(1):45-49
[13] Murillo Pulgarín J A,Fernández L P,García Bermejo L F,Martin Alfonso F. Fast kinetic determination of 1-naphthylacetic acid in commercial formulations,soils,and fruit samples using stopped-flow phosphorimetry.[J]. Journal of Agricultural&Food Chemistry,2003,51(22):6380-6385
[14] Streitwieser A,Husemann M,Kim Y J. Aggregation and reactivity of the dilithium and dicesium enediolates of 1-naphthylacetic acid[J].Journal of Organic Chemistry,2003,68(21):7937-7942
[15]曲亚英,郁继华,陶兴林,常涛. S3307和IBA+NAA浸种对低温胁迫下甜椒幼苗抗冷性的影响[J].甘肃农业大学学报,2006,41(4):52-55
[16]王义强,王启业,华连滩,彭牡丹,钟洁,罗浪.外源K-IBA对北美香柏扦插生根和内源激素含量变化的影响[J].中南林业科技大学学报,2017,37(10):7-12
[17]吴睆迪,严晓萍,陈小丽,申玉香.吲哚丁酸钾、萘乙酸钠对促进多肉根系生长效果研究[J].花卉,2017(12):5-6
[18]张子龙,梁颖. DA-6对水稻种子萌发和幼苗生长的影响[J].西南大学学报(自然科学版),2001,23(3):219-221
[19]匡银近,叶桂萍,覃彩芹.壳寡糖浸种对水稻幼苗抗冷性的影响[J].湖北农业科学,2009,48(7):1568-1571
[20]郑典元,夏依依,丁占平.壳寡糖对水稻幼苗生长及抗寒性能的影响[J].江苏农业科学,2012,40(4):77-79
[21]周金龙,邹少丰,吴江长. S-诱抗素在水稻生产上的应用[J].福建农业,2014(10):89
[22]项洪涛,王彤彤,郑殿峰,王立志,洛育,李琬.孕穗期低温条件下ABA对水稻结实率及叶片生理特性的影响[J].中国农学通报,2016,32(36):16-23
[23] Cai G R,Zuo G Q,Zheng D F,Feng N J. Synthesis of novel 3,3-dimethyl-1-(1H-1,2,4-triazole-1-yl)butan-2-one derivatives and their biological evaluation as plant growth regulators[J]. Chenical Research in Chinese University,2018,25(2):221-228
[24]王学奎,黄见良.植物生理生化试验原理与技术[M].北京:高等教育出版社,2015
[25] Shah K,Kumar R G,Verma S,Dubey R S. Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seeding[J]. Plant Science,2001,161(6):1135-1144
[26] Madore M A. Carbohydrate metabolwasm in photosynthetic and nonphotosynthetic twassues of variegated leaves of Coleus blumei Benth.[J]. Plant Physiology,1990,93(2):617-622
[27]梁喜龙,方淑梅,胡百兴,孔祥森,郑殿峰.烯效唑与胺鲜酯复配浸种对水稻秧苗生长的影响[J].湖北农业科学,2011,50(7):1325-1329
[28]雷舜,刘少君,范亚霖,唐湘如.壮秧剂施用对水稻秧苗形态及生理特征的影响[J].西南农业学报,2016,29(8):1813-1817
[29]张结刚,张美良,潘晓华.不同生长调节物质对机插早稻秧苗生育特性及产量的影响[J].中国农学通报,2015,31(15):151-155
[30]朱广龙,宋成钰,于林林,陈许兵,智文芳,刘泉玮,焦秀荣,周桂生.外源生长调节物质对甜高粱种子萌发过程中盐分胁迫的缓解效应及其生理机制[J].作物学报,2018,44(11):139-150
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