氮高效吸收水稻磷素吸收利用的特点
|
摘要
为探究氮高效水稻磷素吸收利用的基本特点及其与氮素吸收的关系。在大田条件下,于2012-2013年,以114个染色体单片断代换系水稻为供试材料,研究其产量、氮磷吸收利用等性状,并以成熟期吸氮量和产量将供试材料聚类分成6种不同氮效率类型水稻。结果表明,供试群体成熟期吸磷量差异较大,变幅为2. 54～5. 46 g·m~(-2);氮高效水稻成熟期吸磷量显著高于其他氮效率水稻,增幅达8. 99%～47. 24%;氮高效水稻结实期总吸磷量显著高于其他类型水稻,各器官吸磷量也有相似的趋势;氮高效水稻单茎吸磷量、干物质量大;吸磷量影响因子对成熟期吸磷量的贡献表明,结实期吸磷量、穗吸磷量、全株含磷率、单穗吸磷量、吸磷强度均高于同组因子,通径分析与相关分析结果一致;氮高效水稻磷素利用效率除吸磷增量籽粒生产效率显著高于其他类型外,其他各指标均处于中等或较低水平;成熟期吸磷量和吸氮量均对产量有正向促进作用,吸氮量贡献更大。综上,氮高效水稻无论是全株还是各器官成熟期磷素吸收量均较大,结实期表现的更明显,但磷素利用效率中等;氮高效水稻磷素吸收能力强与其单茎吸收量、吸氮强度大有密切关系;氮高效水稻磷素吸收与氮素吸收密切相关。本研究结果为水稻磷素高效吸收利用提供了理论参考。
In order to study the phosphorus( P) absorption and utilization and the relationships between nitrogen( N)absorption and N-efficient in rice,a total of 114 cultivars of chromosome single segment substitution lines( CSSSLs)were planted in 2012 and 2013 under the natural field,to analyze the yield,dry matter production,N absorption and utilization,P absorption and utilization. In addition,these CSSSLs were divided into 6 types by Min SSw( Minimum Square Sum Within Groups) method on the basis of the nitrogen accumulation and yield at the mature stage. These results showed that the N absorption amount during the mature stage was significant difference among six types of rice within the range from 2. 54 to 5. 46 g·m~(-2). The P absorption amount of N-efficient type has increased 8. 99% to 47. 24%compared with other types at the mature stage. The total amount of P absorbed in grain-filling stage of N-efficient type was significantly higher than other types,and similar results were found in different organs. P absorption per tiller and dry matter production of N-efficient type were higher than other types; The correlation analysis of P absorption factors contribution suggested that the phosphorus absorption,spike P absorption,whole plant P content,P absorption per spike and P absorption intensity were higher than those of the same group factor. These results were further confirmed by path analysis. P utilization efficiency of N-efficient type kept at medium or lower levels,except for the yield increase of P absorption increment more than other types. The N absorption amount and P absorption amount at maturity has promoted enhanced yield,and N absorption amount contributed more. In conclusion,whether whole plant or single organ,the P absorption of N-efficient rice are higher than other types,and these were more obvious in grain-filling stage,but the P utilization efficiency has medium level. In addition,the strong ability of N absorption of N-efficient rice is closely related to high P absorption per tiller and high P absorption intensity. A close relationship was found between P absorption and N absorption. This study provides a reference for the efficient absorption and utilization of phosphorus in rice.
In order to study the phosphorus( P) absorption and utilization and the relationships between nitrogen( N)absorption and N-efficient in rice,a total of 114 cultivars of chromosome single segment substitution lines( CSSSLs)were planted in 2012 and 2013 under the natural field,to analyze the yield,dry matter production,N absorption and utilization,P absorption and utilization. In addition,these CSSSLs were divided into 6 types by Min SSw( Minimum Square Sum Within Groups) method on the basis of the nitrogen accumulation and yield at the mature stage. These results showed that the N absorption amount during the mature stage was significant difference among six types of rice within the range from 2. 54 to 5. 46 g·m~(-2). The P absorption amount of N-efficient type has increased 8. 99% to 47. 24%compared with other types at the mature stage. The total amount of P absorbed in grain-filling stage of N-efficient type was significantly higher than other types,and similar results were found in different organs. P absorption per tiller and dry matter production of N-efficient type were higher than other types; The correlation analysis of P absorption factors contribution suggested that the phosphorus absorption,spike P absorption,whole plant P content,P absorption per spike and P absorption intensity were higher than those of the same group factor. These results were further confirmed by path analysis. P utilization efficiency of N-efficient type kept at medium or lower levels,except for the yield increase of P absorption increment more than other types. The N absorption amount and P absorption amount at maturity has promoted enhanced yield,and N absorption amount contributed more. In conclusion,whether whole plant or single organ,the P absorption of N-efficient rice are higher than other types,and these were more obvious in grain-filling stage,but the P utilization efficiency has medium level. In addition,the strong ability of N absorption of N-efficient rice is closely related to high P absorption per tiller and high P absorption intensity. A close relationship was found between P absorption and N absorption. This study provides a reference for the efficient absorption and utilization of phosphorus in rice.
引文
[1]龚金龙,张洪程,李杰,常勇,戴其根,霍中洋,许轲,魏海燕,李德剑,李炳维,沙安勤,周有炎,罗学超,朱镇.施磷量对超级稻南粳44产量和品质的影响[J].中国水稻科学,2011,25(4):447-451
[2]朱兆良,金继运.保障我国粮食安全的肥料问题[J].植物营养与肥料学报,2013,19(2):259-273
[3]高懋芳,邱建军,刘三超,刘宏斌,王立刚,逄焕成.基于文献计量的农业面源污染研究发展态势分析[J].中国农业科学,2014,47(6):1140-1150
[4]徐晓明,张迎信,王会民,程式华,曹立勇.水稻氮、磷、钾吸收利用遗传特征研究进展[J].核农学报,2016,30(4):685-694
[5] Londo J P,Chiang Y C,Hung K H,Chiang T Y,Schaal B A.Phylogeography of Asian wild rice,Oryza rufipogon,reveals multiple independent domestications of cultivated rice, Oryza sativa[J].PNAS,2006,103(25):9578-9583
[6] Zhang H L,Sun J L,Wang M X,Liao D Q,Zeng Y W,Shen S Q,Yu P, Mu P, Wang X K, Li Z C. Genetic structure and phylogeography of rice landraces in Yunnan,China revealed by SSR[J]. Genome,2007,51(1):72-83
[7] Veneklaas E J,Lambers H,Bragg J,Finnegan P M,Lovelock C E,Plaxton W C,Price C A,Scheible W R,Shane M W,White P J,Raven J A. Opportunities for improving phosphorus-use efficiency in crop plants[J]. New Phytologist,2012,195:305-320
[8]吴娜.水稻磷运转蛋白Os PT4的生理功能鉴定[D].南京:南京农业大学,2011
[9] Wang X R,Wang Y X,Tian J,Lim B L,Yan X L,Liao H.Overexpressing At PAP15 enhances phosphorus efficiency in soybean[J]. Plant Physiology,2009,151:233-240
[10]曹璇,赵建宁,李刚,修伟明,赵云丽,王慧,刘惠芬,杨殿林.磷高效转基因水稻磷效率特征分析[J].中国农学通报,2015,31(24):14-18
[11]宋爱梅,黄新朋,孙淑斌,张亚丽,徐国华.氮高效水稻品种苗期耐低磷种质的筛选与鉴定[J].中国水稻科学,2010,24(5):479-486
[12]陈琛,羊彬,朱正康,曹文雅,罗刚,周娟,王祥菊,于小凤,袁秋梅,仲军,王熠,黄建晔,王余龙,董桂春.影响水稻遗传群体株系氮素高效吸收的主要根系性状[J].中国水稻科学,2015,29(4):390-398
[13]陈琛,王熠,羊彬,朱正康,曹文雅,罗刚,周娟,王祥菊,于小凤,袁秋梅,仲军,姚友礼,黄建晔,王余龙,董桂春.株高对遗传群体水稻株系氮素吸收利用的影响[J].中国农业科学,2015,48(22):4450-4459
[14]陈琛,张家星,李万元,唐东南,罗刚,王祥菊,莫兰婧,吕旻珈,周娟,梁国华,黄建晔,王余龙,姚友礼,董桂春.氮高效水稻主要源库性状的基本特点及其调控[J].中国水稻科学,2017,31(2):185-194
[15] Zhou Y,Dong G C,Tao Y J,Chen C,Yang B,Wu Y,Yang Z F,Liang G H,Wang B H,Wang Y L. Mapping quantitative trait loci associated with toot taits using sequencing-based genotyping chromosome segment substitution lines derived from 9311 and Nipponbare in rice(Oryza sativa L.)[J]. PLoS One,2016,11(3):e0151796
[16]顾世梁,莫惠栋.动态聚类的一种新方法—最小组内平方和法[J].江苏农学院学报,1989,10(4):1-8
[17] Singh U,Ladha J K,Castillo E G,Punzalan G,Tirol-Padre A,Duqueza M. Genotypic variation in nitrogen use efficiency in medium-and long-duration rice[J]. Field Crops Research,1998,58:35-53
[18] Koutroubas S D,Ntanos D A. Genotypic differences for grain yield and nitrogen utilization in Indica and Japonica rice under Mediterranean conditions[J]. Field Crops Research,2003,83:251-260
[19]李云春.几种不同类型水稻养分吸收特性及施肥效果研究[D].武汉:华中农业大学,2011
[20]杨雄,马群,张洪程,魏海燕,李国业,李敏,戴其根,霍中洋,许轲,张庆,郭保卫,葛梦洁.不同氮肥水平下早熟晚粳氮和磷的吸收利用特性及相互关系[J].作物学报,2012,38(1):174-180
[21]潘圣刚,黄胜奇,张帆,汪金平,蔡明历,曹凑贵,唐湘如,黎国喜.超高产栽培杂交中籼稻的生长发育特性[J].作物学报,2011,37(3):537-544
[22]韦还和,孟天瑶,李超,张洪程,戴其根,马荣荣,王晓燕,杨筠文.水稻甬优12产量13. 5 t·hm-2以上超高产群体的磷素积累、分配与利用特征[J].作物学报,2016,42(6):886-897
[23]纪洪亭,冯跃华,何腾兵,潘剑,范乐乐,李云,武彪,肖铭,梁显林.超级杂交稻群体干物质和养分积累动态模型与特征分析[J].中国农业科学,2012,45(18):3709-3720
[24] Li M,Zhang H C,Yang X,Ge M J,Ma Q,Wei H Y,Dai Q G,Huo Z Y,Xu K,Luo D Q. Accumulation and utilization of nitrogen,phosphorus and potassium of irrigated rice varieties with high productivities and high N use efficiencies[J]. Field Crops Research,2014,161:55-63
[25]曹利强.不同施氮量对钵苗机插水稻产量形成及氮、磷、钾吸收利用的影响[D].扬州:扬州大学,2015
[26]薛亚光.水稻高产与养分高效利用栽培技术及其生理基础的研究[D].扬州:扬州大学,2013
[27]李鸿伟,杨凯鹏,曹转勤,王志琴,杨建昌.稻麦连作中超高产栽培小麦和水稻的养分吸收与积累特征[J].作物学报,2013,39(3):464-477
[28]董桂春,王熠,于小凤,周娟,彭斌,李进前,田昊,张燕,袁秋梅,王余龙.不同生育期水稻品种氮素吸收利用的差异[J].中国农业科学,2011,44(22):4570-4582
[29]于小凤,王坚纲,李进前,王熠,袁秋梅,仲军,陈琛,田昊,张燕,黄建晔,王余龙,董桂春.不同氮素吸收类型粳稻品种吸氮能力的差异及原因分析[J].中国水稻科学,2012,26(3):331-340
[30]于小凤.氮素高效吸收型水稻的基本特点[D].扬州:扬州大学,2012
[31]毕国俊,杨小雨,刘正辉,丁艳峰.磷对作物产量及品质影响研究进展[EB/OL].(2012-02-28)[2018-05-10]. http://www.paper.edu.cn/releasepaper/content/201202-1058
[32] Hammond J P,Broadley M R,White P J,King G J,Bowen H C,Hayden R,Meancham M C,Mead A,Overs T,Spracklen W P,Greenwood D J. Shoot yield drives phosphorus use efficiency in Brassica oleracea and correlates with root architecture traits[J].Journal of Experimental Botany,2009,60(7):1953-1968
[33]王伟妮,李小坤,鲁剑巍,李慧,鲁明星,戴志刚.氮磷钾配合施用对水稻养分吸收、积累与分配的影响[J].华中农业大学学报,2010,29(6):710-714
[34]叶成.不同氮钾肥处理下转Bt水稻的产量形成及氮钾吸收特征的研究[D].武汉:华中农业大学,2011
[35]刘晓伟,王火焰,朱德进,周健民,陈照明,刘永哲,胡凤琴,缪辰.氮肥施用方式对水稻产量以及氮、磷、钾养分吸收利用的影响[J].南京农业大学学报,2017,40(2):203-210
[36]陈琛.氮高效吸收高产类型遗传群体水稻株系的基本特点[D].扬州:扬州大学,2016
[2]朱兆良,金继运.保障我国粮食安全的肥料问题[J].植物营养与肥料学报,2013,19(2):259-273
[3]高懋芳,邱建军,刘三超,刘宏斌,王立刚,逄焕成.基于文献计量的农业面源污染研究发展态势分析[J].中国农业科学,2014,47(6):1140-1150
[4]徐晓明,张迎信,王会民,程式华,曹立勇.水稻氮、磷、钾吸收利用遗传特征研究进展[J].核农学报,2016,30(4):685-694
[5] Londo J P,Chiang Y C,Hung K H,Chiang T Y,Schaal B A.Phylogeography of Asian wild rice,Oryza rufipogon,reveals multiple independent domestications of cultivated rice, Oryza sativa[J].PNAS,2006,103(25):9578-9583
[6] Zhang H L,Sun J L,Wang M X,Liao D Q,Zeng Y W,Shen S Q,Yu P, Mu P, Wang X K, Li Z C. Genetic structure and phylogeography of rice landraces in Yunnan,China revealed by SSR[J]. Genome,2007,51(1):72-83
[7] Veneklaas E J,Lambers H,Bragg J,Finnegan P M,Lovelock C E,Plaxton W C,Price C A,Scheible W R,Shane M W,White P J,Raven J A. Opportunities for improving phosphorus-use efficiency in crop plants[J]. New Phytologist,2012,195:305-320
[8]吴娜.水稻磷运转蛋白Os PT4的生理功能鉴定[D].南京:南京农业大学,2011
[9] Wang X R,Wang Y X,Tian J,Lim B L,Yan X L,Liao H.Overexpressing At PAP15 enhances phosphorus efficiency in soybean[J]. Plant Physiology,2009,151:233-240
[10]曹璇,赵建宁,李刚,修伟明,赵云丽,王慧,刘惠芬,杨殿林.磷高效转基因水稻磷效率特征分析[J].中国农学通报,2015,31(24):14-18
[11]宋爱梅,黄新朋,孙淑斌,张亚丽,徐国华.氮高效水稻品种苗期耐低磷种质的筛选与鉴定[J].中国水稻科学,2010,24(5):479-486
[12]陈琛,羊彬,朱正康,曹文雅,罗刚,周娟,王祥菊,于小凤,袁秋梅,仲军,王熠,黄建晔,王余龙,董桂春.影响水稻遗传群体株系氮素高效吸收的主要根系性状[J].中国水稻科学,2015,29(4):390-398
[13]陈琛,王熠,羊彬,朱正康,曹文雅,罗刚,周娟,王祥菊,于小凤,袁秋梅,仲军,姚友礼,黄建晔,王余龙,董桂春.株高对遗传群体水稻株系氮素吸收利用的影响[J].中国农业科学,2015,48(22):4450-4459
[14]陈琛,张家星,李万元,唐东南,罗刚,王祥菊,莫兰婧,吕旻珈,周娟,梁国华,黄建晔,王余龙,姚友礼,董桂春.氮高效水稻主要源库性状的基本特点及其调控[J].中国水稻科学,2017,31(2):185-194
[15] Zhou Y,Dong G C,Tao Y J,Chen C,Yang B,Wu Y,Yang Z F,Liang G H,Wang B H,Wang Y L. Mapping quantitative trait loci associated with toot taits using sequencing-based genotyping chromosome segment substitution lines derived from 9311 and Nipponbare in rice(Oryza sativa L.)[J]. PLoS One,2016,11(3):e0151796
[16]顾世梁,莫惠栋.动态聚类的一种新方法—最小组内平方和法[J].江苏农学院学报,1989,10(4):1-8
[17] Singh U,Ladha J K,Castillo E G,Punzalan G,Tirol-Padre A,Duqueza M. Genotypic variation in nitrogen use efficiency in medium-and long-duration rice[J]. Field Crops Research,1998,58:35-53
[18] Koutroubas S D,Ntanos D A. Genotypic differences for grain yield and nitrogen utilization in Indica and Japonica rice under Mediterranean conditions[J]. Field Crops Research,2003,83:251-260
[19]李云春.几种不同类型水稻养分吸收特性及施肥效果研究[D].武汉:华中农业大学,2011
[20]杨雄,马群,张洪程,魏海燕,李国业,李敏,戴其根,霍中洋,许轲,张庆,郭保卫,葛梦洁.不同氮肥水平下早熟晚粳氮和磷的吸收利用特性及相互关系[J].作物学报,2012,38(1):174-180
[21]潘圣刚,黄胜奇,张帆,汪金平,蔡明历,曹凑贵,唐湘如,黎国喜.超高产栽培杂交中籼稻的生长发育特性[J].作物学报,2011,37(3):537-544
[22]韦还和,孟天瑶,李超,张洪程,戴其根,马荣荣,王晓燕,杨筠文.水稻甬优12产量13. 5 t·hm-2以上超高产群体的磷素积累、分配与利用特征[J].作物学报,2016,42(6):886-897
[23]纪洪亭,冯跃华,何腾兵,潘剑,范乐乐,李云,武彪,肖铭,梁显林.超级杂交稻群体干物质和养分积累动态模型与特征分析[J].中国农业科学,2012,45(18):3709-3720
[24] Li M,Zhang H C,Yang X,Ge M J,Ma Q,Wei H Y,Dai Q G,Huo Z Y,Xu K,Luo D Q. Accumulation and utilization of nitrogen,phosphorus and potassium of irrigated rice varieties with high productivities and high N use efficiencies[J]. Field Crops Research,2014,161:55-63
[25]曹利强.不同施氮量对钵苗机插水稻产量形成及氮、磷、钾吸收利用的影响[D].扬州:扬州大学,2015
[26]薛亚光.水稻高产与养分高效利用栽培技术及其生理基础的研究[D].扬州:扬州大学,2013
[27]李鸿伟,杨凯鹏,曹转勤,王志琴,杨建昌.稻麦连作中超高产栽培小麦和水稻的养分吸收与积累特征[J].作物学报,2013,39(3):464-477
[28]董桂春,王熠,于小凤,周娟,彭斌,李进前,田昊,张燕,袁秋梅,王余龙.不同生育期水稻品种氮素吸收利用的差异[J].中国农业科学,2011,44(22):4570-4582
[29]于小凤,王坚纲,李进前,王熠,袁秋梅,仲军,陈琛,田昊,张燕,黄建晔,王余龙,董桂春.不同氮素吸收类型粳稻品种吸氮能力的差异及原因分析[J].中国水稻科学,2012,26(3):331-340
[30]于小凤.氮素高效吸收型水稻的基本特点[D].扬州:扬州大学,2012
[31]毕国俊,杨小雨,刘正辉,丁艳峰.磷对作物产量及品质影响研究进展[EB/OL].(2012-02-28)[2018-05-10]. http://www.paper.edu.cn/releasepaper/content/201202-1058
[32] Hammond J P,Broadley M R,White P J,King G J,Bowen H C,Hayden R,Meancham M C,Mead A,Overs T,Spracklen W P,Greenwood D J. Shoot yield drives phosphorus use efficiency in Brassica oleracea and correlates with root architecture traits[J].Journal of Experimental Botany,2009,60(7):1953-1968
[33]王伟妮,李小坤,鲁剑巍,李慧,鲁明星,戴志刚.氮磷钾配合施用对水稻养分吸收、积累与分配的影响[J].华中农业大学学报,2010,29(6):710-714
[34]叶成.不同氮钾肥处理下转Bt水稻的产量形成及氮钾吸收特征的研究[D].武汉:华中农业大学,2011
[35]刘晓伟,王火焰,朱德进,周健民,陈照明,刘永哲,胡凤琴,缪辰.氮肥施用方式对水稻产量以及氮、磷、钾养分吸收利用的影响[J].南京农业大学学报,2017,40(2):203-210
[36]陈琛.氮高效吸收高产类型遗传群体水稻株系的基本特点[D].扬州:扬州大学,2016