达乌里胡枝子没食子酸提取条件的优化
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摘要
以达乌里胡枝子为对象,研究其没食子酸的最优提取条件,旨在为达乌里胡枝子没食子酸的提取提供依据。结果表明,通过正交试验,达乌里胡枝子没食子酸最佳提取条件为:溶剂浓度60%甲醇,固液比1∶40,超声时间45 min,超声温度60℃;在该条件下,采用高效液相色谱法(HPLC)测定没食子酸的含量(HPLC条件为WaterXBridge C18(250 mm×4.6 mm,5μm),乙腈-0.15%三氟乙酸梯度洗脱,流量为1 m L/min,检测波长为280 nm,柱温30℃),没食子酸在4.91～30.64μg/m L范围内呈现良好的线性关系,平均加样回收率为99.58%,RSD为0.30%;并且利用该方法,测得达乌里胡枝子没食子酸含量为7.57 mg/g。
The optimal extraction conditions of gallic acid in Lespedeza davurica were studied to provide a basis for the extraction of gallic acid in Lespedeza davurica. The results showed that by orthogonal test, the most suitable condition of extracting gallic acid in Lespedeza davurica was gotten, and that was 60% methanol for solvent concentration, solid-liquid ratio of 1∶40, extraction time of 45 min and temperature of 60 ℃. In this condition, gallic acid content was measured with High Performance Liquid Chromatography(HPLC condition was Water XBridge C18(250 mm×4.6 mm,5 μm), and when gradient elution was carried out with it, the flow of acetonitrile-0.15% trifluoroacetic acid was 1 m L/min by detection wavelength of 280 nm under column temperature of 30 ℃), the gallic acid content within 4.91-30.64 μg/m L showed a good linear relationship, with average recovery of 99.58% and 0.30% RSD. And with this method, the gallic acid content in Lespedeza davurica was measured up to 7.57 mg/g.
The optimal extraction conditions of gallic acid in Lespedeza davurica were studied to provide a basis for the extraction of gallic acid in Lespedeza davurica. The results showed that by orthogonal test, the most suitable condition of extracting gallic acid in Lespedeza davurica was gotten, and that was 60% methanol for solvent concentration, solid-liquid ratio of 1∶40, extraction time of 45 min and temperature of 60 ℃. In this condition, gallic acid content was measured with High Performance Liquid Chromatography(HPLC condition was Water XBridge C18(250 mm×4.6 mm,5 μm), and when gradient elution was carried out with it, the flow of acetonitrile-0.15% trifluoroacetic acid was 1 m L/min by detection wavelength of 280 nm under column temperature of 30 ℃), the gallic acid content within 4.91-30.64 μg/m L showed a good linear relationship, with average recovery of 99.58% and 0.30% RSD. And with this method, the gallic acid content in Lespedeza davurica was measured up to 7.57 mg/g.
引文
[1]王艳.额济纳绿洲胡杨叶片酚类物质含量变化对外来损害的响应研究[D].北京:北京林业大学,2009.
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[5]郝文芳,杜润峰,王龙飞.达乌里胡枝子抗氧化防御系统对增强UV-B辐射的动态响应[J].草地学报,2013,20(2):419-427.
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[8]杜金友,陈晓阳,胡东南,等.干旱胁迫条件下几种胡枝子渗透物质变化的研究[J].华北农学报,2004,19(Z1):40-44.
[9]尚作华,霍金海,王伟明.HPLC法测定核桃楸叶中没食子酸的含量[J].黑龙江中医药,2014,43(5):67-68.
[10]金欣,王锋,姚岚,等.HPLC测定野老鹳草中没食子酸和鞣花酸的总量[J].中成药,2010,32(7):1172-1176.
[11]袁永兵,张兰珍,郭亚健,等.RP-HPLC法测定叶下珠中没食子酸、柯里拉京和鞣花酸的含量[J].北京中医药大学学报,2009,32(1):56-58.
[12]陈晓静,何杰,杨建云,等.RP-HPLC法同时测定葡萄籽提取物中没食子酸、儿茶素和表儿茶素的含量[J].药物分析杂志,2015(4):723-727.
[13]关潇滢,胡晓谕,温晓丽,等.高效液相色谱法同时测定六味补血胶囊中没食子酸、芍药苷和藁本内酯含量[J].中国药业,2018(5):13-15.
[14]金玲,杜鸿灵,张旭.HPLC波长切换法同时测定正本清茶中没食子酸、绿原酸和栀子苷的含量[J].亚太传统医药,2018(1):27-31.
[15]王佳慧,于欣悦,廖声玲,等.树莓中鞣花酸提取工艺的优化[J].中国林副特产,2017(4):14-17.
[16]刘忠英,高展,罗赛,等.普洱茶没食子酸的提取分离工艺研究[J].西南师范大学学报(自然科学版),2015,40(4):47-52.
[17]黄斌,金晨,廖辉,等.多指标优选维药古丽娜提取工艺及含量测定研究[J].中华中医药杂志,2018(1):353-356.
[2]KRAUS T E,YU Z,PRESTON C M,et al.Linking chemical reactivity and protein precipitation to structural characteristics of foliar tannins[J].Journal of Chemical Ecology,2003,29(3):703-730.
[3]邓业成,骆海玉,张明,等.银杏外种皮酚酸类物质的抑菌活性[J].河南农业科学,2010(2):64-66.
[4]徐宗海,吴洪新,魏孝义,等.达乌里胡枝子中黄酮类化学成分的研究[J].西北植物学报,2010,30(7):1485-1489.
[5]郝文芳,杜润峰,王龙飞.达乌里胡枝子抗氧化防御系统对增强UV-B辐射的动态响应[J].草地学报,2013,20(2):419-427.
[6]BORRONI G,CAZZANIGA M,ANDREOLETTI M,et al.Low glomerular filtration rate is a risk factor for ribavirin-associated anaemia in old patients with chronic hepatitis C[J].Journal of Viral Hepatitis,2013,20(4):90-95.
[7]李肖玲,崔岚,祝德秋.没食子酸生物学作用的研究进展[J].中国药师,2004,7(10):767-769.
[8]杜金友,陈晓阳,胡东南,等.干旱胁迫条件下几种胡枝子渗透物质变化的研究[J].华北农学报,2004,19(Z1):40-44.
[9]尚作华,霍金海,王伟明.HPLC法测定核桃楸叶中没食子酸的含量[J].黑龙江中医药,2014,43(5):67-68.
[10]金欣,王锋,姚岚,等.HPLC测定野老鹳草中没食子酸和鞣花酸的总量[J].中成药,2010,32(7):1172-1176.
[11]袁永兵,张兰珍,郭亚健,等.RP-HPLC法测定叶下珠中没食子酸、柯里拉京和鞣花酸的含量[J].北京中医药大学学报,2009,32(1):56-58.
[12]陈晓静,何杰,杨建云,等.RP-HPLC法同时测定葡萄籽提取物中没食子酸、儿茶素和表儿茶素的含量[J].药物分析杂志,2015(4):723-727.
[13]关潇滢,胡晓谕,温晓丽,等.高效液相色谱法同时测定六味补血胶囊中没食子酸、芍药苷和藁本内酯含量[J].中国药业,2018(5):13-15.
[14]金玲,杜鸿灵,张旭.HPLC波长切换法同时测定正本清茶中没食子酸、绿原酸和栀子苷的含量[J].亚太传统医药,2018(1):27-31.
[15]王佳慧,于欣悦,廖声玲,等.树莓中鞣花酸提取工艺的优化[J].中国林副特产,2017(4):14-17.
[16]刘忠英,高展,罗赛,等.普洱茶没食子酸的提取分离工艺研究[J].西南师范大学学报(自然科学版),2015,40(4):47-52.
[17]黄斌,金晨,廖辉,等.多指标优选维药古丽娜提取工艺及含量测定研究[J].中华中医药杂志,2018(1):353-356.