华支睾吸虫病在我国的流行及诊断方法研究进展
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摘要
华支睾吸虫病是由华支睾吸虫引起的危害严重的鱼源性人兽共患寄生虫病,主要引起人和多种动物的肝胆病变,严重者可引起胆管上皮癌,因此华支睾吸虫被国际癌症署(IARC)归为第Ⅰ类生物性致癌因子。我国华支睾吸虫病主要流行于淡水资源丰富的地区,在我国27个省、市、自治区均有该病人体感染的报道。近年来,我国华支睾吸虫感染率呈上升趋势,且受威胁地区和人数不断增加。对该病的诊断和防控已刻不容缓,传统的病原学诊断方法存在着一定的局限性和弊端。免疫学方法和分子生物学技术的不断发展为该病的诊断提供了新的途径,今后在华支睾吸虫病的基础研究与防控中起到更加重要的作用。论文就华支睾吸虫病的流行特点及诊断方法研究进展进行综述,以期为华支睾吸虫病的研究提供参考。
Clonorchiasis caused by Clonorchis sinensis is an important fish-borne zoonotic parasitic disease and predominantly leads to hepatobiliary diseases.The most severe complication is associated with cholangiocarcinoma(CCA).So C.sinensis was reclassified as groupⅠ bio-carcinogen by the International Agency of Cancer Research.Clonorchiasis is mainly prevalent in areas with abundant freshwater resources in China,and human infection of Clonorchis sinensis has been reported in 27 provinces,cities and autonomous regions.In recent years, the infection rates are rising and the threatened area and numbers are increasing of Clonorchis sinensisis in China.It is urgent that the diagnosis,prevention and control of the disease be strengthen. The traditional etiology diagnosis methods of clonorchiasis exist some limitations and shortcomings.The continuous development of immunological methods and molecular biology techniques provide new ways,it play a more important role in the basis research and prevention and control of clonorchiosis in the future.Herein,this paper aims to sum up the recent development progress of epidemiological characteristics and diagnostic methods of clonorchiasis and provide theoretical reference and basis for readers.
Clonorchiasis caused by Clonorchis sinensis is an important fish-borne zoonotic parasitic disease and predominantly leads to hepatobiliary diseases.The most severe complication is associated with cholangiocarcinoma(CCA).So C.sinensis was reclassified as groupⅠ bio-carcinogen by the International Agency of Cancer Research.Clonorchiasis is mainly prevalent in areas with abundant freshwater resources in China,and human infection of Clonorchis sinensis has been reported in 27 provinces,cities and autonomous regions.In recent years, the infection rates are rising and the threatened area and numbers are increasing of Clonorchis sinensisis in China.It is urgent that the diagnosis,prevention and control of the disease be strengthen. The traditional etiology diagnosis methods of clonorchiasis exist some limitations and shortcomings.The continuous development of immunological methods and molecular biology techniques provide new ways,it play a more important role in the basis research and prevention and control of clonorchiosis in the future.Herein,this paper aims to sum up the recent development progress of epidemiological characteristics and diagnostic methods of clonorchiasis and provide theoretical reference and basis for readers.
引文
[1] 倪兴维,犹银俊,李洁,等.华支睾吸虫病诊断方法研究进展[J].当代畜牧,2014(4):48-49.
[2] Lai D H,Hong X K,Su B X,et al.Current status of Clonorchis sinensis and clonorchiasis in China [J].Trans R Soc Trop Med Hyg,2016,110:21-27.
[3] 刘刚,刘孝刚.华支睾吸虫病流行病学研究进展[J].现代畜牧兽医,2009(11):54-56.
[4] Tang Z L,Huang Y,Yu X B.Current status and perspectives of Clonorchis sinensis and clonorchiasis:epidemiology,pathogenesis,omics,prevention and control [J].Infect Dis Poverty,2016,71(5):1-12.
[5] Chen Y D,Zhou C H,Xu L Q.Analysis of the results of two nationwide surveys on Clonorchis sinensis infection in China[J].Biomed Environ Sci,2012,25(2):163-166.
[6] 钱门宝,陈颖丹,周晓农.从认识到实践-纪念华支睾吸虫发现140周年[J].中国寄生虫学与寄生虫病杂志,2014,32(4):247-252.
[7] Sithithaworn P,Andrews R H,Nguyen V D,et al.The current status of opisthorchiasis and clonorchiasis in the Mekong Basin[J].Parasitol Int,2012,61(1):10-16.
[8] 孙青松.华支睾吸虫抗原基因的特性鉴定[D].吉林长春:吉林大学,2014.
[9] Li H M,Qian M B,Yang Y C,et al.Performance evaluation of existing immunoassays for Clonorchis sinensis infection in China [J].Parasit Vector,2018,35(11):1-7.
[10] Chen T J,Jiang H Y,Sun H C,et al.Sequence analysis and characterization of pyruvate kinase from Clonorchis sinensis,a 53.1-kDa homopentamer,implicated immune protective efficacy against clonorchiasis [J].Parasit Vector,2017,10:557.
[11] Cheng N,Xu XN,Zhou Y,et al.Cs1,a Clonorchis sinensis-derived serodiagnostic antigen containing tandem repeats and a signal peptide [J].PLoS Negl Trop Dis,2018,12 (8):e000-6683.
[12] Kim J G,Ahn C S,Sripa B,et al.Clonorchis sinensis omega-class glutathione transferases are reliable biomarkers for serodiagnosis of clonorchiasis and opisthorchiasis[J].Clin Microbiol Infect,2018,9(18):30320-30323.
[13] 濮珏彪,唐莉莉,殷岑楠,等.华支睾吸虫钙结合蛋白Cs16的重组表达及血清诊断应用的初步评价[J].中国寄生虫学与寄生虫病杂志,2018,3(36):275-279.
[14] Kim Y J,Yoo W G,Lee M R,et al.Molecular and structural characterization of the tegumental 20.6-kDa protein in Clonorchis sinensis as a potential druggable Target [J].Int J Mol Sci,2017,18(3).pii:E557.
[15] Wu Z,Tang Z,Shang M,et al.Comparative analysis of immune effects in mice model:Clonorchis sinensis cysteine protease generated from recombinant Escherichia coli and Bacillus subtilis spores [J].Parasitol Res,2017,116(7):1811-1822.
[16] Park S Y,Jeong M S,Park S A,et al.Structural basis of the cystein protease inhibitor Clonorchis sinensis Stefin-1[J].Biochem Biophys Res Commun,2018,498(1):9-17.
[17] 孙青松,于妮娜,尚信池,等.华支睾吸虫抗原CsSQ1和CsSQ2的免疫学虫体定位[J].中国兽医科学,2017,47(10):1299-1302.
[18] 陈莹,杨齐,吴尚为.PCR及其衍生技术在华支睾吸虫检测中的应用[J].热带医学杂志,2015,15(8):1154-1157.
[19] Muller B,Schmidt J,Mehlhorn H.PCR diagnosis of infections with different species of Opisthorchiidae using a rapid clean-up procedure for stool samples and specific primers[J].Parasitol Res,2007,100(4):905-909.
[20] Sato M,Thaenkham U,Dekumyoy P,et al.Discrimination of O.viverrini,C.sinensis,H.pumilio and H.taichui using nuclear DNA-based PCR targeting ribosomal DNA ITS regions[J].Acta Trop,2009,109(1):81-83.
[21] Jeong J Y,Lee J Y,Chung B S,et al.A new method for estimating the prevalence of clonorchiasis in Korea-A proposal to replace arbitrary riverside sampling [J].Medicine,2017,96(13):1-8.
[22] Kim E M,Verweij J J,Jalili A,et al.Detection of Clonorchis sinensis in stool samples using real-time PCR [J].Ann Trop Med Parasitol,2009,103(6):513-518.
[23] Qiao T,Zheng P M,Ma R H,et al.Development of a real-time PCR assay for the detection of Clonorchis sinensis DNA in gallbladder bile and stone samples from patients with cholecystolithiasis [J].Parasitol Res,2012,111(4):1497-1503.
[24] Wang D,Korhonen P K,Gasser R B,et al.Improved genomic resources and new bioinformatic workflow for the carcinogenic parasite Clonorchis sinensis:Biotechnological implications [J].Biotechnol Adv,2018,6(4):894-904.
[25] 蒋智华,杨庆利,杨益超.基于线粒体细胞色素C氧化酶亚单位1 基因鉴别华支睾吸虫和扇棘单睾吸虫的双重PCR 法的建立[J].中国寄生虫学与寄生虫病杂志,2018,36(2):187-189.
[26] Yang Y,Li M,Pan C,Yang Y,et al.A duplex PCR for the simultaneous detection of Fasciola hepatica and Clonorchis sinensis [J].Vet Parasitol,2018,259:1-5.
[27] Chen Y,Wen T,Lai D H,et al.Development and evaluation of loop-mediated isothermal amplification(LAMP)for rapid detection of Clonorchis sinensis from its first intermediate hosts,freshwater snails [J].Parasitology,2013,140(11):1377-1383.
[28] Kim E M,Kwak Y S,YI M H,et al.Clonorchis sinensis antigens alter hepatic macrophage polarization in vitro and in vivo[J].PLoS Negl Trop Dis,2017,11(5):e0005614.
[29] Rahman S M M,Song H B,Jin Y,et al.Application of a loop-mediated isothermal amplification (LAMP) assay targeting cox1gene for the detection of Clonorchis sinensis in human fecal samples[J].PLoS Negl Trop Dis,2017,11(10):e0005995.
[2] Lai D H,Hong X K,Su B X,et al.Current status of Clonorchis sinensis and clonorchiasis in China [J].Trans R Soc Trop Med Hyg,2016,110:21-27.
[3] 刘刚,刘孝刚.华支睾吸虫病流行病学研究进展[J].现代畜牧兽医,2009(11):54-56.
[4] Tang Z L,Huang Y,Yu X B.Current status and perspectives of Clonorchis sinensis and clonorchiasis:epidemiology,pathogenesis,omics,prevention and control [J].Infect Dis Poverty,2016,71(5):1-12.
[5] Chen Y D,Zhou C H,Xu L Q.Analysis of the results of two nationwide surveys on Clonorchis sinensis infection in China[J].Biomed Environ Sci,2012,25(2):163-166.
[6] 钱门宝,陈颖丹,周晓农.从认识到实践-纪念华支睾吸虫发现140周年[J].中国寄生虫学与寄生虫病杂志,2014,32(4):247-252.
[7] Sithithaworn P,Andrews R H,Nguyen V D,et al.The current status of opisthorchiasis and clonorchiasis in the Mekong Basin[J].Parasitol Int,2012,61(1):10-16.
[8] 孙青松.华支睾吸虫抗原基因的特性鉴定[D].吉林长春:吉林大学,2014.
[9] Li H M,Qian M B,Yang Y C,et al.Performance evaluation of existing immunoassays for Clonorchis sinensis infection in China [J].Parasit Vector,2018,35(11):1-7.
[10] Chen T J,Jiang H Y,Sun H C,et al.Sequence analysis and characterization of pyruvate kinase from Clonorchis sinensis,a 53.1-kDa homopentamer,implicated immune protective efficacy against clonorchiasis [J].Parasit Vector,2017,10:557.
[11] Cheng N,Xu XN,Zhou Y,et al.Cs1,a Clonorchis sinensis-derived serodiagnostic antigen containing tandem repeats and a signal peptide [J].PLoS Negl Trop Dis,2018,12 (8):e000-6683.
[12] Kim J G,Ahn C S,Sripa B,et al.Clonorchis sinensis omega-class glutathione transferases are reliable biomarkers for serodiagnosis of clonorchiasis and opisthorchiasis[J].Clin Microbiol Infect,2018,9(18):30320-30323.
[13] 濮珏彪,唐莉莉,殷岑楠,等.华支睾吸虫钙结合蛋白Cs16的重组表达及血清诊断应用的初步评价[J].中国寄生虫学与寄生虫病杂志,2018,3(36):275-279.
[14] Kim Y J,Yoo W G,Lee M R,et al.Molecular and structural characterization of the tegumental 20.6-kDa protein in Clonorchis sinensis as a potential druggable Target [J].Int J Mol Sci,2017,18(3).pii:E557.
[15] Wu Z,Tang Z,Shang M,et al.Comparative analysis of immune effects in mice model:Clonorchis sinensis cysteine protease generated from recombinant Escherichia coli and Bacillus subtilis spores [J].Parasitol Res,2017,116(7):1811-1822.
[16] Park S Y,Jeong M S,Park S A,et al.Structural basis of the cystein protease inhibitor Clonorchis sinensis Stefin-1[J].Biochem Biophys Res Commun,2018,498(1):9-17.
[17] 孙青松,于妮娜,尚信池,等.华支睾吸虫抗原CsSQ1和CsSQ2的免疫学虫体定位[J].中国兽医科学,2017,47(10):1299-1302.
[18] 陈莹,杨齐,吴尚为.PCR及其衍生技术在华支睾吸虫检测中的应用[J].热带医学杂志,2015,15(8):1154-1157.
[19] Muller B,Schmidt J,Mehlhorn H.PCR diagnosis of infections with different species of Opisthorchiidae using a rapid clean-up procedure for stool samples and specific primers[J].Parasitol Res,2007,100(4):905-909.
[20] Sato M,Thaenkham U,Dekumyoy P,et al.Discrimination of O.viverrini,C.sinensis,H.pumilio and H.taichui using nuclear DNA-based PCR targeting ribosomal DNA ITS regions[J].Acta Trop,2009,109(1):81-83.
[21] Jeong J Y,Lee J Y,Chung B S,et al.A new method for estimating the prevalence of clonorchiasis in Korea-A proposal to replace arbitrary riverside sampling [J].Medicine,2017,96(13):1-8.
[22] Kim E M,Verweij J J,Jalili A,et al.Detection of Clonorchis sinensis in stool samples using real-time PCR [J].Ann Trop Med Parasitol,2009,103(6):513-518.
[23] Qiao T,Zheng P M,Ma R H,et al.Development of a real-time PCR assay for the detection of Clonorchis sinensis DNA in gallbladder bile and stone samples from patients with cholecystolithiasis [J].Parasitol Res,2012,111(4):1497-1503.
[24] Wang D,Korhonen P K,Gasser R B,et al.Improved genomic resources and new bioinformatic workflow for the carcinogenic parasite Clonorchis sinensis:Biotechnological implications [J].Biotechnol Adv,2018,6(4):894-904.
[25] 蒋智华,杨庆利,杨益超.基于线粒体细胞色素C氧化酶亚单位1 基因鉴别华支睾吸虫和扇棘单睾吸虫的双重PCR 法的建立[J].中国寄生虫学与寄生虫病杂志,2018,36(2):187-189.
[26] Yang Y,Li M,Pan C,Yang Y,et al.A duplex PCR for the simultaneous detection of Fasciola hepatica and Clonorchis sinensis [J].Vet Parasitol,2018,259:1-5.
[27] Chen Y,Wen T,Lai D H,et al.Development and evaluation of loop-mediated isothermal amplification(LAMP)for rapid detection of Clonorchis sinensis from its first intermediate hosts,freshwater snails [J].Parasitology,2013,140(11):1377-1383.
[28] Kim E M,Kwak Y S,YI M H,et al.Clonorchis sinensis antigens alter hepatic macrophage polarization in vitro and in vivo[J].PLoS Negl Trop Dis,2017,11(5):e0005614.
[29] Rahman S M M,Song H B,Jin Y,et al.Application of a loop-mediated isothermal amplification (LAMP) assay targeting cox1gene for the detection of Clonorchis sinensis in human fecal samples[J].PLoS Negl Trop Dis,2017,11(10):e0005995.