妊娠期慢性疼痛对母鼠认知功能影响机制研究

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英文篇名：RESEARCH ON THE IMPACT AND MECHANISM OF CHRONIC PAIN DURING PREGNANCY ON COGNITIVE FUNCTION IN FEMALE MICE 作者：周芳 ; 黄悦 ; 李清 ; 涂淑敏 ; 刘玉平 英文作者：ZHOU Fang;HUANG Yue;LI Qing;TU Shu-Min;LIU Yu-Ping;School of Nursing, Xuzhou Medical University;Department of Anesthesiology, Beijing Tsinghua Changgeng Hospital;Department of Nursing, The Af?liated Hospital of Xuzhou Medical University; 关键词：妊娠期 ; 慢性疼痛 ; 优裕胎教 ; 认知功能 ; 机制 英文关键词：Pregnancy;;Chronic pain;;Enriched environmental intervention;;Cognitive function;;Mechanism 中文刊名：ZTYZ 英文刊名：Chinese Journal of Pain Medicine 机构：徐州医科大学护理学院;北京清华长庚医院麻醉科;徐州医科大学附属医院护理部; 出版日期：2019-03-15 出版单位：中国疼痛医学杂志 年：2019 期：v.25 基金：国家自然科学基金资助项目(No.81400894);; 江苏省2016年“青蓝工程”中青年学术带头人基金资助 语种：中文; 页：ZTYZ201903008 页数：4 CN：03 ISSN：11-3741/R 分类号：23-26

摘要

目的:研究妊娠期慢性疼痛对孕鼠认知功能的影响及其分子机制。方法:选取40只同批成功受孕小鼠,采用随机数字表法分为四组(n=10):正常对照组、假手术对照组、慢性疼痛组、慢性疼痛+优裕胎教组。采用酶联免疫吸附法分别在不同时间点检测各组孕鼠血清中5-羟色胺(5-HT)和多巴胺(DA)水平并进行比较分析。结果:与假手术对照组相比,慢性疼痛组孕鼠学习能力和记忆能力显著降低,且在各时间点5-HT、DA水平显著降低(P<0.05)。与慢性疼痛组孕鼠相比,慢性疼痛+优裕胎教组孕鼠学习能力和记忆能力显著增强,且在各时间点5-HT、DA水平显著升高(P<0.05)。结论:5-HT和DA分泌异常可能是慢性疼痛导致孕鼠认知障碍的分子机制,优裕胎教可通过改变上述递质的分泌从而有效改善孕鼠认知功能。
        Objective: To study the impact of chronic pain during pregnancy on cognitive function in female mice and its molecular mechanism. Methods: Forty pregnant mice in the same batch were randomly divided into four groups(n=10 each group): normal, sham, chronic pain(CP), CP+enriched environmental intervention(EEI). The enzyme-linked immunosorbent assay was used to detect the levels of serotonin(5-HT) and dopamine(DA) in the serum of pregnant mice at different time points. Results: Compared to sham group, the learning and memory ability of pregnant mice in CP group was remarkably decreased, and the levels of 5-HT and DA were signi?cantly decreased at each time point(P<0.05). But compared to CP group, the learning and memory ability in CP+EEI group was signi?cantly increased, and the levels of 5-HT and DA were signi?cantly increased at corresponding time points(P<0.05). Conclusion: The abnormal secretion of 5-HT and DA may be related to the mechanism of cognitive impairment caused by chronic pain in pregnant mice, while EEI can effectively improve the cognitive function and restore 5-HT and DA secretion.

引文

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