糖尿病大鼠皮肤溃疡阴证模型
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摘要
糖尿病足溃疡在糖尿病并发症中较为常见且具有高的致死率,引起国内外专家关注。随着研究的深入,中西医在临床中发现一类症状相似糖尿病足溃疡久治难愈,符合中医学的阴证疮面,故称糖尿病足溃疡阴证。要想对糖尿病足溃疡阴证的发病机制,病理特点和干预治疗等多方面有深入的了解,动物模型的建立为其提供可靠有效的途径。论文概括了前人对于糖尿病大鼠皮肤溃疡模型的进展,同时总结其阴证模型的优缺点和自己的经验与认识,以期对临床具有指导意义的糖尿病大鼠皮肤溃疡阴证模型。
Diabetic foot ulcers are common in diabetic complications and have a high mortality,which has attracted the attention of experts at home and abroad.With the in-depth study of Chinese and Western medicine found in the clinical symptoms of a similar type of diabetic foot ulcers for a long time to cure,in line with the traditional Chinese medicine side of the Yin syndrome,so Chinese medicine called diabetic foot ulcers Yin syndrome.To understand the pathogenesis,pathological features and interventional treatment of diabetic foot ulcer syndrome,a mature animal model is established to provide a reliable and effective way.This article summarized the progress of predecessors for the diabetic rat skin ulcer model,and the advantages and disadvantages of its Yin syndrome model and its own experience in order to seek a clinically instructive model of skin ulcer ulceration in diabetic rats.
Diabetic foot ulcers are common in diabetic complications and have a high mortality,which has attracted the attention of experts at home and abroad.With the in-depth study of Chinese and Western medicine found in the clinical symptoms of a similar type of diabetic foot ulcers for a long time to cure,in line with the traditional Chinese medicine side of the Yin syndrome,so Chinese medicine called diabetic foot ulcers Yin syndrome.To understand the pathogenesis,pathological features and interventional treatment of diabetic foot ulcer syndrome,a mature animal model is established to provide a reliable and effective way.This article summarized the progress of predecessors for the diabetic rat skin ulcer model,and the advantages and disadvantages of its Yin syndrome model and its own experience in order to seek a clinically instructive model of skin ulcer ulceration in diabetic rats.
引文
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[5]宋丹,冉丽媛,姜如娇,等.糖尿病研究中的动物模型进展[J].中国比较医学杂志,2016,26(9):83-87.
[6] Seaton M,Hocking A,Gibran N S.Porcine models of cutaneous wound healing[J].ILAR J,2015,56(1):127-138.
[7] Ryan A E,Laura C,Michael H.Autoantigen-induced focusing of Vb13+T cells precedes onset of autoimmune diabetes in the LEW.1WR1rat[J].Diabetics,2014,63(2):594-604.
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[12] Kitada M,Ogura Y,Koya D.Rodent models of diabetic nephropathy:their utility and limitations[J].Int J Nephrol Renovasc Dis.2016,9:279-290.
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[14] Lepantalo M,Apelqvist J,Setacci C,et al.Chapter V:diabetic foot[J].Eur J Vasc Endovasc Surg,2011,42(S2):S60-S74.
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[16] Gao S,Shen Y,Geng F,et al.Research process on the animal models and treatment strategies of diabetic foot ulcer[J].Zhejiang Da Xue Xue BaoYi Xue Ban,2017,46(1):97-105.
[17] Chuan T,Haojie H,Lei X,et al.Hypoxia pretreatment of bone marrow-derived mesenchymal stem cells seeded in a collagen-chitosan sponge scaffold promotes skin wound healing in diabetic rats with hindlimb ischemia[J].Wound Rep Reg,2015,2016(4):45-56.
[18] Duscher D,Neofytou E,Wong V W,et al.Transdermal deferoxamine prevents pressure-induced diabetic ulcers[J].Proceed Natl Acad Sci,2015,112(1):94-99.
[19] Jin S,Zhang M,Gao Y,et al.The efficacy of Jing Wan Hong ointment for nerve injury diabetic foot ulcer and its mechanisms[J].J Diabet Res,2014,2014:1-9.
[20] Kato J,Kamiya H,Himeno T,et al.Mesenchymal stem cells ameliorate impaired wound healing through enhancing keratinocyte functions in diabetic foot ulcerations on the plantar skin of rats[J].J Diabet Complicat,2014,28(5):588-595.
[21] Danigo A,Nasser M,Bessaguet F,et al.Candesartan restores pressure-induced vasodilation and prevents skin pressure ulcer formation in diabetic mice[J].Cardiovasc Diabetol,2015,14(1):1-12.
[22] Nunan R,Harding K G,Martin P.Clinical challenges of chronic wounds:searching for an optimal animal model to recapitulate their complexity[J].Dis Mod Mechan,2014,7(11):1205-1213.
[23]Wang X,Ge J,Tredget E E,et al.The mouse excisional wound splinting model,including applications for stem cell transplantation[J].Nat Protoc,2013,8(2):302-309.
[24] Tong C,Hao HJ,Lei X,et al.Hypoxia pretreatment of bone marrow-derived mesenchymal stem cells seeded in a collagenchitosan sponge scaffold promotes skin wound healing in diabetic rats with hindlimb ischemia[J].Wound Repair Regen,2016,24(1):45-56.
[25]Wang X,Ge J,Tredget E E,et al.The mouse excisional wound splinting model,,including applications for stem cell transplantation[J].Nat Protoc,2013,8(2):302-309.
[26] Chereddy K K,Lopes A,Koussoroplis S,et al.Combined effects of PLGA and vascular endothelial growth factor promote the healing of non-diabetic and diabetic wound[J].Nanomedicine,2015,11(8):1975-1984.
[27]阙华发,邢捷.补益气血方剂对大鼠慢性难愈性创面血管内皮生长因子及血管生成的影响[J].中西医结合学报,2008,10(6):1049-1053.
[28]孙彦辉,孙永辉,孙立虹,等.温和灸对大鼠慢性难愈合创面组织修复微循环的影响[J].针刺研究,2011,36(5):321-326.
[29]贾连城,吕培文.吕培文应用回阳生肌法治疗慢性难愈性皮肤溃疡经验总结[J].中国医药导报,2015,12(28):85-88.
[30]陈红风.中医外科学[M].北京:人民卫生出版社,2012.
[31]李媛.糖尿病慢性皮肤溃疡阴证模型建立与回阳生肌膏对糖尿病阴证溃疡创面不同时相的影响[D].北京:北京中医药大学,2014.
[32]蒙玉娇.回阳生肌膏拆方对db/db小鼠慢性皮肤溃疡创面愈合及巨噬细胞表型转化的影响[C]//2016年中华中医药学会外科分会学术年会论文,辽宁丹东:中华中医药学会,2016:7.
[2] Boulton A J M,De Groot L J,Chrousos G,et al.The diabetic foot[J].Endotext,2016,10(26):1-49.
[3] Armstrong D G,Boulton A J,Bus S A.Diabetic foot ulcers and their recurrence[J].N Engl J Med,2017,376:2367-2375.
[4] Skovs S.Modeling type 2diabetes in rats using high fat diet and streptozotocin[J].J Diabet Invest,2014,5(4):349-358.
[5]宋丹,冉丽媛,姜如娇,等.糖尿病研究中的动物模型进展[J].中国比较医学杂志,2016,26(9):83-87.
[6] Seaton M,Hocking A,Gibran N S.Porcine models of cutaneous wound healing[J].ILAR J,2015,56(1):127-138.
[7] Ryan A E,Laura C,Michael H.Autoantigen-induced focusing of Vb13+T cells precedes onset of autoimmune diabetes in the LEW.1WR1rat[J].Diabetics,2014,63(2):594-604.
[8] Amin A,Krisztina K,Médea V,et al.Experimental diabetes mellitus in different animal models[J].J Diabet Res,2016,8(9):1-12.
[9] Yorek M A.Alternatives to the streptozotoc in-diabetic rodent[J].Sci Direct,2016,127:89-112.
[10] Chatzigeorgiou A,Halapas A,Kalafatakis K,et al.The use of animal models in the study of diabetes mellitus[J].Vivo,2009,23(2):245-258.
[11] Munehiro K,Yoshio O,Daisuke K,et al.Rodent models of diabetic nephropathy:their utility and limitations[J].Int J Nephrol Renovasc Dis,2016,9:279-290.
[12] Kitada M,Ogura Y,Koya D.Rodent models of diabetic nephropathy:their utility and limitations[J].Int J Nephrol Renovasc Dis.2016,9:279-290.
[13] Goyal S N,Reddy N M,Patil K R,et al.Challenges and issues with streptozotocin-induced diabetes-A clinically relevant animal model to understand the diabetes pathogenesis and evaluate therapeutics[J].Chem Biol Int,2016,244:49-63.
[14] Lepantalo M,Apelqvist J,Setacci C,et al.Chapter V:diabetic foot[J].Eur J Vasc Endovasc Surg,2011,42(S2):S60-S74.
[15]韩强,李守伟,柳国斌.糖尿病足实验动物模型研究进展[J].上海中医药杂志,2014,48(5):117-121.
[16] Gao S,Shen Y,Geng F,et al.Research process on the animal models and treatment strategies of diabetic foot ulcer[J].Zhejiang Da Xue Xue BaoYi Xue Ban,2017,46(1):97-105.
[17] Chuan T,Haojie H,Lei X,et al.Hypoxia pretreatment of bone marrow-derived mesenchymal stem cells seeded in a collagen-chitosan sponge scaffold promotes skin wound healing in diabetic rats with hindlimb ischemia[J].Wound Rep Reg,2015,2016(4):45-56.
[18] Duscher D,Neofytou E,Wong V W,et al.Transdermal deferoxamine prevents pressure-induced diabetic ulcers[J].Proceed Natl Acad Sci,2015,112(1):94-99.
[19] Jin S,Zhang M,Gao Y,et al.The efficacy of Jing Wan Hong ointment for nerve injury diabetic foot ulcer and its mechanisms[J].J Diabet Res,2014,2014:1-9.
[20] Kato J,Kamiya H,Himeno T,et al.Mesenchymal stem cells ameliorate impaired wound healing through enhancing keratinocyte functions in diabetic foot ulcerations on the plantar skin of rats[J].J Diabet Complicat,2014,28(5):588-595.
[21] Danigo A,Nasser M,Bessaguet F,et al.Candesartan restores pressure-induced vasodilation and prevents skin pressure ulcer formation in diabetic mice[J].Cardiovasc Diabetol,2015,14(1):1-12.
[22] Nunan R,Harding K G,Martin P.Clinical challenges of chronic wounds:searching for an optimal animal model to recapitulate their complexity[J].Dis Mod Mechan,2014,7(11):1205-1213.
[23]Wang X,Ge J,Tredget E E,et al.The mouse excisional wound splinting model,including applications for stem cell transplantation[J].Nat Protoc,2013,8(2):302-309.
[24] Tong C,Hao HJ,Lei X,et al.Hypoxia pretreatment of bone marrow-derived mesenchymal stem cells seeded in a collagenchitosan sponge scaffold promotes skin wound healing in diabetic rats with hindlimb ischemia[J].Wound Repair Regen,2016,24(1):45-56.
[25]Wang X,Ge J,Tredget E E,et al.The mouse excisional wound splinting model,,including applications for stem cell transplantation[J].Nat Protoc,2013,8(2):302-309.
[26] Chereddy K K,Lopes A,Koussoroplis S,et al.Combined effects of PLGA and vascular endothelial growth factor promote the healing of non-diabetic and diabetic wound[J].Nanomedicine,2015,11(8):1975-1984.
[27]阙华发,邢捷.补益气血方剂对大鼠慢性难愈性创面血管内皮生长因子及血管生成的影响[J].中西医结合学报,2008,10(6):1049-1053.
[28]孙彦辉,孙永辉,孙立虹,等.温和灸对大鼠慢性难愈合创面组织修复微循环的影响[J].针刺研究,2011,36(5):321-326.
[29]贾连城,吕培文.吕培文应用回阳生肌法治疗慢性难愈性皮肤溃疡经验总结[J].中国医药导报,2015,12(28):85-88.
[30]陈红风.中医外科学[M].北京:人民卫生出版社,2012.
[31]李媛.糖尿病慢性皮肤溃疡阴证模型建立与回阳生肌膏对糖尿病阴证溃疡创面不同时相的影响[D].北京:北京中医药大学,2014.
[32]蒙玉娇.回阳生肌膏拆方对db/db小鼠慢性皮肤溃疡创面愈合及巨噬细胞表型转化的影响[C]//2016年中华中医药学会外科分会学术年会论文,辽宁丹东:中华中医药学会,2016:7.