慢性乙型肝炎患者外周血CD_8~+T细胞表面抑制性受体及血清IL-10、TGF-β表达
|
摘要
目的探讨慢性乙型肝炎患者外周血CD8+T细胞表面抑制性受体及血清白细胞介素10 (IL-10)、转化生长因子-β(TGF-β)表达。方法选取2016年1月—2018年6月慢性乙型肝炎患者90例作为病例组,根据患者病情分为慢性乙型肝炎病毒(HBV)携带者30例(携带组)、慢性乙型肝炎轻度30例(轻度组)与中重度30例(中重度组)。另选择健康体检者30例为对照组。采用流式细胞仪检测各组外周血CD8+T细胞表面抑制性受体细胞毒T淋巴细胞相关抗原4 (CTLA-4)、程序性死亡受体(PD-1)、淋巴细胞活化基因3 (LAG-3)表达情况,采用ELISA方法检测血清IL-10、TGF-β表达水平。结果病例组外周血CD8+T细胞CTLA-4(32.1%±3.5%)、PD-1 (4.5%±0.7%)、LAG-3 (46.4%±4.8%)水平显著高于对照组,差异有统计学意义(P<0.05);乙肝中重度组高于携带组、轻度组,差异有统计学意义(P<0.05)。乙肝组血清IL-10[(131.4±14.9)pg/ml]与TGF-β[(79.1±12.3) pg/ml]水平显著高于对照组,差异有统计学意义(P<0.05);中重度组高于携带组与轻度组,差异有统计学意义(P<0.05)。结论慢性乙型肝炎患者外周血CD8+T细胞表面抑制性受体及血清IL-10、TGF-β表达升高,并且随着病情的加重升高越明显。
Objective To discuss the expression of CD_8~+T cell surface inhibitory receptors and serum IL-10 and TGF-beta in patients with chronic hepatitis B. Methods Ninety patients with chronic hepatitis B diagnosed in hospital from January 2016 to June 2018 were selected as case group, and they were divided into three groups according to the patient's condition: HBV carrier group, mild group and moderate and severe group, 30 cases in each group. Thirty healthy persons were selected as the control group. The expression levels of CD_8~+T cell surface inhibitory receptors including CTLA-4,PD-1, LAG-3 in peripheral blood were detected by flow cytometry, and serum IL-10 and TGF-beta were detected by ELISA assay. Results The levels of CTLA-4(32.1%±3.5%), PD-1(4.5%±0.7%), LAG-3(46.4%±4.8%) in peripheral blood CD_8~+T cells of case group were significantly higher than those of control group(P<0.05); the levels of CTLA-4, PD-1 and LAG-3 in moderate and severe group were higher than those in HBV carrier group and mild group(P<0.05). The serum levels of IL-10 [(131.4±14.9) pg/ml] and TGF-β [(79.1±12.3) pg/ml] in case group were significantly higher than those in control group(P<0.05); and the serum levels of IL-10 and TGF-beta in moderate and severe group were higher than those in HBV carrier group and mild group(P<0.05).Conclusion The expressionslevels of CD_8~+T cell surface inhibitory receptors and serum IL-10,TGF-β in patients with chronic hepatitis B were increased with disease severity.
Objective To discuss the expression of CD_8~+T cell surface inhibitory receptors and serum IL-10 and TGF-beta in patients with chronic hepatitis B. Methods Ninety patients with chronic hepatitis B diagnosed in hospital from January 2016 to June 2018 were selected as case group, and they were divided into three groups according to the patient's condition: HBV carrier group, mild group and moderate and severe group, 30 cases in each group. Thirty healthy persons were selected as the control group. The expression levels of CD_8~+T cell surface inhibitory receptors including CTLA-4,PD-1, LAG-3 in peripheral blood were detected by flow cytometry, and serum IL-10 and TGF-beta were detected by ELISA assay. Results The levels of CTLA-4(32.1%±3.5%), PD-1(4.5%±0.7%), LAG-3(46.4%±4.8%) in peripheral blood CD_8~+T cells of case group were significantly higher than those of control group(P<0.05); the levels of CTLA-4, PD-1 and LAG-3 in moderate and severe group were higher than those in HBV carrier group and mild group(P<0.05). The serum levels of IL-10 [(131.4±14.9) pg/ml] and TGF-β [(79.1±12.3) pg/ml] in case group were significantly higher than those in control group(P<0.05); and the serum levels of IL-10 and TGF-beta in moderate and severe group were higher than those in HBV carrier group and mild group(P<0.05).Conclusion The expressionslevels of CD_8~+T cell surface inhibitory receptors and serum IL-10,TGF-β in patients with chronic hepatitis B were increased with disease severity.
引文
[1] Wang L, Zou ZQ, Liu CX, et al. Immunotherapeutic in-terventions in chronic hepatitis B virus infection:a review[J]. J Immunol Methods, 2014,407:1-8.
[2]何丽.不同临床表现HBV感染者CD8+T细胞抑制性受体、T-bet和IFN-γ表达水平分析[D].第四军医大学,2014:1-65.
[3]中华医学会传染病与寄生虫病学分会、肝病学分会.病毒性肝炎防治方案[J].中华内科杂志,2001,40(1):62-68.
[4]彭文丽,毕小云.血清可溶性共刺激因子CTLA-4和CD28在乙型肝炎患者血清中的表达及意义[J].第三军医大学学报,2013,35(1):82-84.
[5]郭莲娣,王丹,唐子执,等.乙型肝炎病毒抑制肝细胞免疫检查点pd-1配体基因表达的研究[J].重庆医学,2017,46(27):3752-3754,3758.
[6]夏红,周智,杨玉霞.乙型肝炎患者恩替卡韦抗病毒治疗后外周血T淋巴细胞PD-1的表达及其与HBeAg血清学的关系[J].海南医学院学报,2016,22(7):655-657.
[7]任利霞,孟延红.拉米夫定联合干扰素α-2a治疗对乙型肝炎患者PD-1及Toll-4表达的影响[J].中国当代医药,2016,23(14):112-114,117.
[8]范德环,张学光,奚沁华,等.人可溶性PD-1在肝病患者血清中表达检测及临床意义[J].免疫学杂志,2015,31(9):799-802.
[9] Tian X, Zhang A, Qiu C, et al. The upregulation ofLAG-3 on T cells defines a subpopulation with functionalexhaustion and correlates with disease progression in HIV-infected subjects[J]. J Immunol, 2015,194(8):3873-3882.
[10] Koch K, Koch N, Sandaradura de Silva U, et al. In-creased frequency of CD49b/LAG-3+type 1 regulatory Tcells in HIV-infected individuals[J]. AIDS Res Hum Ret-roviruses, 2015,31(12):1238-1246.
[11]马晨芸,陆志成,王克翔,等.慢性乙型肝炎病毒感染患者外周血CD8+T淋巴细胞表面淋巴细胞活化基因3水平升高[J].细胞与分子免疫学杂志,2016,32(4):532-537.
[12]董月皎,李雪芬,崔大伟,等.细胞抑制性受体PD-1及LAG-3在慢性乙型肝炎患者CD4+T细胞表面的表达及其意义[J].临床检验杂志,2016,34(2):100-102.
[13]臧唯,王琢,张阔野.TGF-β和IL-6在乙型肝炎患者血清中的表达意义[J].吉林医学,2018,39(9):1643-1644.
[14]赵阳,姜晓明,赵臣.血清HBV-DNA、ALT、AST、TNF-α和IL-10联合检测在慢性乙型肝炎诊断中的意义[J].中国实验诊断学,2018,22(7):1181-1182.
[15]刘桂玲,李连花,牛梦铱,等.IL-10与IL-17水平在乙型肝炎病毒相关肝癌中的表达及临床价值研究[J].中华医院感染学杂志,2017,27(16):3627-3630.
[2]何丽.不同临床表现HBV感染者CD8+T细胞抑制性受体、T-bet和IFN-γ表达水平分析[D].第四军医大学,2014:1-65.
[3]中华医学会传染病与寄生虫病学分会、肝病学分会.病毒性肝炎防治方案[J].中华内科杂志,2001,40(1):62-68.
[4]彭文丽,毕小云.血清可溶性共刺激因子CTLA-4和CD28在乙型肝炎患者血清中的表达及意义[J].第三军医大学学报,2013,35(1):82-84.
[5]郭莲娣,王丹,唐子执,等.乙型肝炎病毒抑制肝细胞免疫检查点pd-1配体基因表达的研究[J].重庆医学,2017,46(27):3752-3754,3758.
[6]夏红,周智,杨玉霞.乙型肝炎患者恩替卡韦抗病毒治疗后外周血T淋巴细胞PD-1的表达及其与HBeAg血清学的关系[J].海南医学院学报,2016,22(7):655-657.
[7]任利霞,孟延红.拉米夫定联合干扰素α-2a治疗对乙型肝炎患者PD-1及Toll-4表达的影响[J].中国当代医药,2016,23(14):112-114,117.
[8]范德环,张学光,奚沁华,等.人可溶性PD-1在肝病患者血清中表达检测及临床意义[J].免疫学杂志,2015,31(9):799-802.
[9] Tian X, Zhang A, Qiu C, et al. The upregulation ofLAG-3 on T cells defines a subpopulation with functionalexhaustion and correlates with disease progression in HIV-infected subjects[J]. J Immunol, 2015,194(8):3873-3882.
[10] Koch K, Koch N, Sandaradura de Silva U, et al. In-creased frequency of CD49b/LAG-3+type 1 regulatory Tcells in HIV-infected individuals[J]. AIDS Res Hum Ret-roviruses, 2015,31(12):1238-1246.
[11]马晨芸,陆志成,王克翔,等.慢性乙型肝炎病毒感染患者外周血CD8+T淋巴细胞表面淋巴细胞活化基因3水平升高[J].细胞与分子免疫学杂志,2016,32(4):532-537.
[12]董月皎,李雪芬,崔大伟,等.细胞抑制性受体PD-1及LAG-3在慢性乙型肝炎患者CD4+T细胞表面的表达及其意义[J].临床检验杂志,2016,34(2):100-102.
[13]臧唯,王琢,张阔野.TGF-β和IL-6在乙型肝炎患者血清中的表达意义[J].吉林医学,2018,39(9):1643-1644.
[14]赵阳,姜晓明,赵臣.血清HBV-DNA、ALT、AST、TNF-α和IL-10联合检测在慢性乙型肝炎诊断中的意义[J].中国实验诊断学,2018,22(7):1181-1182.
[15]刘桂玲,李连花,牛梦铱,等.IL-10与IL-17水平在乙型肝炎病毒相关肝癌中的表达及临床价值研究[J].中华医院感染学杂志,2017,27(16):3627-3630.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |