血清Leptin、sBO-R及TNF-α在猫脂肪肝综合征中的表达及临床意义

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英文篇名：Clinical significance about the expression of serum Leptin,sBO-R and TNF-α in feline hepatic lipidosis 作者：陆江 ; 姜忠华 ; 朱道仙 ; 卢劲晔 ; 刘静 ; 卢炜 英文作者：LU Jiang;JIANG Zhonghua;ZHU Daoxian;LU Jinye;LIU Jing;LU Wei;Jiangsu Agri-animal Husbandry vocational College;Kangmei pet hospital in Nan tong; 关键词：猫脂肪肝综合征 ; 瘦素 ; 可溶性瘦素受体 ; TNF-α ; 肝脏脂肪含量 英文关键词：Feline Hepatic Lipidosis;;Leptin;;sBO-R;;TNF-α;;liver fat content 中文刊名：XMYS 英文刊名：Animal Husbandry & Veterinary Medicine 机构：江苏农牧科技职业学院;南通市康美宠物医院; 出版日期：2018-12-10 出版单位：畜牧与兽医 年：2018 期：v.50;No.401 基金：江苏高校品牌专业建设工程资助项目(PPZY2015C230) 语种：中文; 页：XMYS201812024 页数：4 CN：12 ISSN：32-1192/S 分类号：109-112

摘要

为了研究瘦素(Leptin)、可溶性瘦素受体(sBO-R)、肿瘤坏死因子α(TNF-α)等因子在猫脂肪肝综合征(FHL)中的作用机制,将收集的33例猫脂肪肝综合征作为试验组,并以27例健康体检猫作为对照组,检测血清中丙氨酸氨基转移酶活性(ALT)、总胆红素素(TBIL)和甘油三酯(TG)、Leptin、sBO-R、TNF-α及肝脏脂肪含量(LFC),分析LFC和TG与其他因子的相关性,经多元逐步回归建立线性方程,并建立了Leptin和TNF-α的ROC曲线。结果显示,与对照组比较,试验组血清中ALT、TBIL、TG均升高(P<0.05),Leptin、TNF-α升高极为明显(P<0.01),sBO-R降低(P<0.05),LFC升高(9.32 vs 4.11);相关性分析显示,LFC与TG、ALT、TBIL、Leptin、TNF-α呈正相关,与sBOR呈负相关;多元逐步线性回归显示血清Leptin、TNF-α是脂肪肝和高脂血症的独立危险因素,sBO-R为脂肪肝保护因素,ROC曲线显示Leptin、TNF-α对FHL诊断的敏感性高(90.9%、81.8%),特异性强(92.6%、89.9%)。结果表明,Leptin和TNF-α与FHL密切关联,可能是导致FHL发生发展的重要原因,而且可作为FHL早期诊断的候选指标。
        In order to study the mechanism of Leptin,sBO-R,TNF-αin Feline Hepatic Lipidosis(FHL),the 33 cases of FHL were collected as experimental group,and the 27 cases of healthy check-up cat as control group.The serum alanine transaminase activity(ALT),total bilirubin(TBIL),triglyceride(TG),Leptin,soluble Leptin receptor(sBO-R) and Tumor Necrosis Factor alpha(TNF-α) and liver fat content(LFC),TG were detected.correlations with LFC and other factors was analysised,the linear equation was established by multiple regression step by step,and the ROC curves of Leptin and TNF-αwere established.Results showed,the expression of ALT,TBIL and TG were all increased in serum of the experimental group compared with the control group(P<0.05),the Leptin and TNF-α were significantly increased(P<0.01),sBO-R was decreased(P<0.05),and LFC was increased(9.32 vs 4.11).Correlation analysis showed that LFC was positively correlated with TG,ALT,TBIL,Leptin and TNF-α,and negatively correlated with sBO-R.Multiple stepwise linear regression showed that serumLeptin and TNF-αwere independent risk factors for FHL and hyperlipemia,sBO-R was protective factor for FHL,and ROC curve showed that Leptin and TNF-αwere highly sensitive to FHL diagnosis(90.9%,81.8%),and had strong specificity(92.6%,89.9%).The results show that Leptin and TNF-αare closely associated with FHL,which may be the important causes of the occurrence and development in FHL,and can be used as a candidate index for the early diagnosis of FHL.

引文

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