基于rpl16序列分析大百合的遗传多样性及遗传结构
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摘要
大百合是百合科大百合属一种重要的野生植物资源,本研究采用基因测序法,利用叶绿体DNA rpl16序列对10个大百合居群进行了遗传多样性和遗传结构分析,旨在为其资源保护和开发利用提供理论基础。结果显示:rpl16序列矩阵长度为699 bp,共检测到14个变异位点和12个单倍型,单倍型多态性(Hd)为0.604;总的遗传多样性(HT)为0.660。该居群间遗传变异(71.53%)大于居群内遗传变异(28.47%),说明居群间变异是其居群的主要变异来源;居群间遗传分化很高(Gst=0.677,Nst=0.614,Fst=0.71531),基因流较低(Nm=0.0995)。失配分析结果表明种群在进化过程中曾经历过快速扩张,中性检验支持上述结果:Tajima′s D=-1.74368(P>0.05);Fu and Li′s D=-2.69366(P<0.05);Fu and Li′s F=-2.79896(P<0.05)。研究结果表明:大百合居群遗传多样性较高,居群间存在较高的遗传分化,主要与其生活史特征相关。基于得到的居群遗传信息,建议采取就地保护为主的保护策略。
Cardiocrinum giganteum belongs to the genus Cardiocrinum(Endl.)Lindl.(Liliaceae),which is an important wild plant resource.In this study,the genetic diversity and genetic structure of 10 C.giganteum populations were analyzed by using the rpl16 sequence of the chloroplast DNA in order to provide theoretical basis for its resource protection and utilization.By alignment of 699 bp sequence,14 mutation sites and 12 haplotypes were identified,with the haplotype polymorphism(Hd)and the total genetic diversity(HT)of 0.604 and 0.660,respectively.The genetic variation among the population(71.53%)was higher than the intra-population genetic variation(28.47%),indicating the population variation largely contributed by different populations.The genetic differentiation among the population was high(Gst=0.677,Nst=0.614,Fst=0.71531)and the gene flow was low(Nm=0.0995).Mismatch analysis revealed that the population had experienced rapid expansion during the evolution process,which was supported by the neutrality tests(Tajima′s D=-1.74368(P>0.05);Fu and Li′s D=-2.69366(P<0.05);Fu and Li′s F=-2.79896(P<0.05)).The study revealed the abundant genetic diversity and differentiation of C.giganteum particularly among populations,which was mainly related to the characteristics of life history.Thus,this work suggested an in-situ conservation strategy being employed in C.giganteum.
Cardiocrinum giganteum belongs to the genus Cardiocrinum(Endl.)Lindl.(Liliaceae),which is an important wild plant resource.In this study,the genetic diversity and genetic structure of 10 C.giganteum populations were analyzed by using the rpl16 sequence of the chloroplast DNA in order to provide theoretical basis for its resource protection and utilization.By alignment of 699 bp sequence,14 mutation sites and 12 haplotypes were identified,with the haplotype polymorphism(Hd)and the total genetic diversity(HT)of 0.604 and 0.660,respectively.The genetic variation among the population(71.53%)was higher than the intra-population genetic variation(28.47%),indicating the population variation largely contributed by different populations.The genetic differentiation among the population was high(Gst=0.677,Nst=0.614,Fst=0.71531)and the gene flow was low(Nm=0.0995).Mismatch analysis revealed that the population had experienced rapid expansion during the evolution process,which was supported by the neutrality tests(Tajima′s D=-1.74368(P>0.05);Fu and Li′s D=-2.69366(P<0.05);Fu and Li′s F=-2.79896(P<0.05)).The study revealed the abundant genetic diversity and differentiation of C.giganteum particularly among populations,which was mainly related to the characteristics of life history.Thus,this work suggested an in-situ conservation strategy being employed in C.giganteum.
引文
[1]中国科学院中国植物志编辑委员会.中国植物志:第十四卷.北京:科学出版社,1980:157ChinesefloraeditorialboardoftheChineseAcademy ofSciences.Florareipublicaepopularissinicae:Volume14.Beijing:Science Press,1980:157
[2]Matthews V A.The genus Cardiocrinum in cultivation.The plantsman,2002,1(4):192-205
[3]冯正波,张超.花中新贵大百合.植物杂志,2001(3):22Feng Z B,Zhang C.Cardiocrinum giganteum,new nobility of flowers.Plant Journal,2001(3):22
[4]孙国峰.百合王子——“大百合”.中国花卉盆景,2002,(11):10-11Sun G F.Lily prince-Cardiocrinum giganteum.China Flower&Penjing,2002(11):10-11
[5]裴盛基,龙春林.应用民族植物学.昆明:云南民族出版社,1998:156-158PeiSJ,LongCL.Appliedethnobotany.Kunming:The Nationalities Publishing House of Yunnan,1998:156-158
[6]Hayashik K,Kawano S.Bulbous monocots native to Japan and adjacent areas-their habitats,life histories and phylogeny.Acta Horticulture.2005,673:43-58
[7]李守丽.大百合属间杂交亲和性、离体培养及耐热生理研究.北京:中国科学院研究生院,2006Li S L.Research on hybridization compatibility,tissue culture andheattoleranceCardiocrinumgiganteum.Beijing:The Institute of Botany,Chinese Academy of Sciences,2006
[8]林凤琼,朱昌叁,黎素平.大百合与百合属优良品种杂交试验.南方农业学报,2012,43(11):1733-1736LinFQ,ZhuCS,LiSP.Hybridizationresearchon Cardiocrinum giganteum and elite varieties of Lilium.Journal of Southern Agriculture,2012,43(11):1733-1736
[9]张金政,龙雅宜,孙国峰.大百合的生物多样性及其引种观察.园艺学报,2002,29(5):462-466Zhang J Z,Long Y Y,Sun G F.Biodiversity of Cardiocrinum giganteumandobservationduringitsintroduction.Acta Horticulturae Sinica,2002,29(5):462-466
[10]关文灵,李枝林,黄建新.野生花卉大百合的引种栽培.北方园艺,2003(4):33Guan W L,Li Z L,Huang J X.Introduction and cultivation of wild Cardiocrinum giganteum.Northern Horticulture,2003(4):33
[11]喻晓,文涛,曾杨.大百合RAPD体系的建立与优化.西南农业学报,2007,20(6):1304-1306YuX,WenT,Zeng Y.Establishmentandoptimizationof Cardiocrinumgiganteum′sRAPDsystem.SouthwestChina Journal of Agricultural Sciences,2007,20(6):1304-1306
[12]李彦坤,高亦珂.大百合属植物开发价值研究.现代园艺,2015(10):106-107LiYK,GaoYK.Studyonthedevelopmentvalueof Cardiocrinumgiganteum.XiandaiHorticulture,2015(10):106-107
[13]王云生,黄宏文,王瑛.植物分子群体遗传学研究动态.遗传,2007,29(10):1191-1198Wang YS,HuangHW,Wang Y.Recentprogressinplant molecular population genetics.Hereditas,2007,29(10):1191-1198
[14]葛颂,洪德元.濒危物种裂叶沙参及其近缘广布种泡沙参的遗传多样性研究.遗传学报,1999,26(4):410-417GeS,HongDY.Studiesofmorphologicalandallozyme variationoftheendangered Adenophoralobophyllaandits widespread congener A.potaninii.Acta Genetica Sinica,1999,26(4):410-417
[15]Soltis D E,Soltis P S.Choosing an approach and an appropriate geneforphylogeneticanalysis.SoltisDE,SoltisP S,Doyle J J. Molecular Systematics of PlantsⅡ:DNA Squence. Boston:Kluwer Academic Publishers,1998
[16]王容升,魏鑫,曹立荣.基于叶绿体基因多样性的中国水稻起源进化研究.植物遗传资源学报,2011,12(5):686-693Wang R S,Wei X,Cao L R.Origin and evolution of cultivated rice(O.sativaL)inChinabasedongenediversityof chloroplast genome.Joumal of Plant Genetic Resources,2011,12(5):686-693
[17]Downie S R,Katz-Downie D S.A molecular phylogeny of Apiaceae subfamily Apioideae:evidence from ribosomal DNA internaltranscribedspacersequence.AmericanJournalof Botany,1996,83(2):234-251
[18]何薇.基于叶绿体序列rpl16的中国樱桃居群遗传多样性及居群遗传结构研究.成都:四川农业大学,2014HeW.Studiesongeneticdiversityandpopulationgenetic structure of Chinese cherry resource(Cerasus pseudocerasus Lindl.)based on cpDNA rpl16.Chengdu:Sichuan Agricultural University,2014
[19]彭铁成,徐芳,罗静瑶,陈云云,汤华.基于叶绿体基因的香蕉遗传多样性研究.热带生物学报,2012,3(4):293-294Peng T C,Xu F,Luo J Y,Chen Y Y,Tang H.Genetic diversity of banana varieties based on genes from chloroplast.Journal of Tropical Organisms,2012,3(4):293-294
[20]郭会芳,阚显照,张韧,陈鸿姗.nrDNA ITS和cpDNA rpl16基因测序法对六种鼠尾草的检测.植物分类与资源学报,2008,30(3):345-350Guo H F,Kan X Z,Zhang R,Chen H S.Identification of SalviaspeciesbynrDNAITSandcpDNArpl16sequenceanalyses.Plant Diversity and Resources,2008,30(3):345-350
[21]Jordan WC,CourtneyM W,NeigelJE.Lowlevelsof intraspecific genetic variation at a rapidly evolving chloroplast DNAlocusinnorth Americanduckweeds(Lemnaceae).American Journal of Botany,1996,83:430-439
[22]Doyle J J,Doyle J L.A rapid DNA isolation procedure for small quantities of fresh leaf tissue.Phytochemical Bulletin,1987,19(1):11-15
[23]Hall T A.BioEdit:a user friendly biological sequence alignment editorandanalysisprogramfor Windows95/98/NT.Nucleic Acids Symposium Series,1999,41:95-98
[24]Thompson J D,Gibson T J,Plewniak F,Higgins D G.The ClustalXwindowsinterface:flexiblestrategiesformultiple sequencealignmentaidedbyqualityanalysistools.Nucleic Acids Research,1997,25(24):4876-4882
[25]RaymondM,PoussetF.Anexacttestforpopulation differentiation.Evolution,1995,49(6):1280-1283
[26]赵玉倩,丁瑞华,李玉帆,刘正权,贾桂霞.中国特有植物南川百合群体遗传多样性研究.分子植物育种,2016,14(3):749-755Zhao YQ,DingRH,Li YF,LiuZQ,JiaGX.Studieson geneticdiversityofLiliumrosthorniiDielspopulations,the endemicplantsofChina.MolecularPlantBreeding,2016,14(3):749-755
[27]Tajima F.Statistical method for testing the neutral mutation hypothesis by DNA polymorphism.Genetics,1989,123(3):585-595
[28]Fu Y X,Li W H.Statistical tests of neutrality of mutations.Genetics,1993,133(3):693-709
[29]Excoffier L,Laval G,Schneider S.Arlequin(version 3):an integrated software package for population genetics data analysis.Evolutionary Bioinformatics Online,2005,1(4A):47-50
[30]张成林,郭智慧,张新全,白且史,张昌平,李平,马啸.利用SSR标记对垂穗披碱草和老芒麦进行物种鉴定和遗传变异分析.植物遗传资源学报,2016,17(3):416-422ZhangCL,GuoZH,ZhangXQ,BaiQS,ZhangCP,LiP,MaX.Ananalysisofspeciesidentificationandgenetic divergence of drooping wildrye(Elymus nutans)and Siberian wildrye(E.sibiricus)by SSR markers.Journal of P1ant Genetic Resources,2016,17(3):416-422
[31]Brown A H D,Clegg M T,Kahler A L.Plant population genetics,breeding and genetics resources.Bioscience,1990,41(1):613-614
[32]Loveless M D,Hamrick J L.Ecological determinants of genetic structure in plant populations.Annual Review of Ecology and Systematics,1984,15:65-95
[33]Petit R J,Duminil J,Fineschi S.Compative organization ofchloroplast,mitochondrialandnucleardiversityinplant populations.Molecular Ecology,2005,14(3):689-701
[34]刘春香,李钧敏,李红丽,罗芳丽.匍匐茎克隆植物蛇莓的遗传多样性和遗传结构.江苏农业科学,2016,44(2):215-220Liu C X,Li J M,Li H L,Luo F L.Genetic diversity and genetic structure of creeping stem clone plant mock-strawberry.Jiangsu Agricultural Sciences,2016,44(2):215-220
[35]陈俊秋,慈秀芹,李巧明,李捷.樟科濒危植物思茅木姜子遗传多样性的ISSR分析.生物多样性,2006,14(5):410-420ChenJQ,CiXQ,LiQM,LiJ.GeneticdiversityofLitsea szemaois,anendangeredspeciesendemictoChina,detected by inter-simple sequence repeat(ISSR).Biodiversity Science,2006,14(5):410-420
[36]关文灵,李叶芳,陈贤.大百合自然繁殖特性研究.园艺学报,2009,36(S):2056Guan W L,Li Y F,Chen X.Study on the natural propagation characteristics of Cardiocrinum giganteum.Acta Horticulturae Sinica,2009,36(S):2056
[37]Buso G S C,Rangel P H,Ferreira M E.Analysis of genetic variabilityofSouth Americanwildfleepopulations(Oryza glumaepatula)with isozymes and RAPD markers.Molecular Ecology,1998,7(1):107-117
[38]Bussell J D.The distribution of random amplified polymorphic DNA(RAPD)diversity amongst populations of Isotoma perraea(Lobeliaceae).Molecular Ecology,1999,8(5):775-789
[39]段青,崔光芬,王祥宁,王继华,贾文杰,马璐琳,吴丽芳.云南泸定百合遗传多样性的表型与ISSR分析.西北植物学报,2013,33(6):1106-1113Duan Q,Cui G F,Wang X N,Wang J H,Jia W J,Ma L L,Wu L F.Genetic diversity of Lilium sargentiae based on phenotypic traitsandSSRmarkers.ActaBotanicaBoreali-Occidentalia Sinica,2013,33(6):1106-1113
[40]吴祝华.百合属部分种亲缘关系与岷江百合群体遗传结构研究.南京:南京农业大学,2008Wu Z H.Research on genetic relationship conservation genetics of Lilium species and of L.regale.Nanjing:Nanjing Agricultural University,2008
[41]熊勇,陈名红,熊华斌.百合psb A-trn H基因序列变异及遗传多样性分析.生物技术,2013,23(2):53-56Xiong Y,ChenMH,XiongHB.Geneticdiversityanalysis ofLiliumbasedonpsb A-trn Hgenesequencevariation.Biotechnology,2013,23(2):53-56
[42]Viglant L,Stoneking M,Harpending H,Hawkes K,Wilson A C.African populations and the evolution of human mitoehondrial DNA. Science,1991,253(5027):1503-1507
[43]伍丹.四川大百合繁殖及遗传多样性研究.成都:四川农业大学,2006WuD.Studyonreproductionandgeneticdiversityof CardiocrinumgiganteuminSichuan.Chengdu:Sichuan Agricultural University,2006
[2]Matthews V A.The genus Cardiocrinum in cultivation.The plantsman,2002,1(4):192-205
[3]冯正波,张超.花中新贵大百合.植物杂志,2001(3):22Feng Z B,Zhang C.Cardiocrinum giganteum,new nobility of flowers.Plant Journal,2001(3):22
[4]孙国峰.百合王子——“大百合”.中国花卉盆景,2002,(11):10-11Sun G F.Lily prince-Cardiocrinum giganteum.China Flower&Penjing,2002(11):10-11
[5]裴盛基,龙春林.应用民族植物学.昆明:云南民族出版社,1998:156-158PeiSJ,LongCL.Appliedethnobotany.Kunming:The Nationalities Publishing House of Yunnan,1998:156-158
[6]Hayashik K,Kawano S.Bulbous monocots native to Japan and adjacent areas-their habitats,life histories and phylogeny.Acta Horticulture.2005,673:43-58
[7]李守丽.大百合属间杂交亲和性、离体培养及耐热生理研究.北京:中国科学院研究生院,2006Li S L.Research on hybridization compatibility,tissue culture andheattoleranceCardiocrinumgiganteum.Beijing:The Institute of Botany,Chinese Academy of Sciences,2006
[8]林凤琼,朱昌叁,黎素平.大百合与百合属优良品种杂交试验.南方农业学报,2012,43(11):1733-1736LinFQ,ZhuCS,LiSP.Hybridizationresearchon Cardiocrinum giganteum and elite varieties of Lilium.Journal of Southern Agriculture,2012,43(11):1733-1736
[9]张金政,龙雅宜,孙国峰.大百合的生物多样性及其引种观察.园艺学报,2002,29(5):462-466Zhang J Z,Long Y Y,Sun G F.Biodiversity of Cardiocrinum giganteumandobservationduringitsintroduction.Acta Horticulturae Sinica,2002,29(5):462-466
[10]关文灵,李枝林,黄建新.野生花卉大百合的引种栽培.北方园艺,2003(4):33Guan W L,Li Z L,Huang J X.Introduction and cultivation of wild Cardiocrinum giganteum.Northern Horticulture,2003(4):33
[11]喻晓,文涛,曾杨.大百合RAPD体系的建立与优化.西南农业学报,2007,20(6):1304-1306YuX,WenT,Zeng Y.Establishmentandoptimizationof Cardiocrinumgiganteum′sRAPDsystem.SouthwestChina Journal of Agricultural Sciences,2007,20(6):1304-1306
[12]李彦坤,高亦珂.大百合属植物开发价值研究.现代园艺,2015(10):106-107LiYK,GaoYK.Studyonthedevelopmentvalueof Cardiocrinumgiganteum.XiandaiHorticulture,2015(10):106-107
[13]王云生,黄宏文,王瑛.植物分子群体遗传学研究动态.遗传,2007,29(10):1191-1198Wang YS,HuangHW,Wang Y.Recentprogressinplant molecular population genetics.Hereditas,2007,29(10):1191-1198
[14]葛颂,洪德元.濒危物种裂叶沙参及其近缘广布种泡沙参的遗传多样性研究.遗传学报,1999,26(4):410-417GeS,HongDY.Studiesofmorphologicalandallozyme variationoftheendangered Adenophoralobophyllaandits widespread congener A.potaninii.Acta Genetica Sinica,1999,26(4):410-417
[15]Soltis D E,Soltis P S.Choosing an approach and an appropriate geneforphylogeneticanalysis.SoltisDE,SoltisP S,Doyle J J. Molecular Systematics of PlantsⅡ:DNA Squence. Boston:Kluwer Academic Publishers,1998
[16]王容升,魏鑫,曹立荣.基于叶绿体基因多样性的中国水稻起源进化研究.植物遗传资源学报,2011,12(5):686-693Wang R S,Wei X,Cao L R.Origin and evolution of cultivated rice(O.sativaL)inChinabasedongenediversityof chloroplast genome.Joumal of Plant Genetic Resources,2011,12(5):686-693
[17]Downie S R,Katz-Downie D S.A molecular phylogeny of Apiaceae subfamily Apioideae:evidence from ribosomal DNA internaltranscribedspacersequence.AmericanJournalof Botany,1996,83(2):234-251
[18]何薇.基于叶绿体序列rpl16的中国樱桃居群遗传多样性及居群遗传结构研究.成都:四川农业大学,2014HeW.Studiesongeneticdiversityandpopulationgenetic structure of Chinese cherry resource(Cerasus pseudocerasus Lindl.)based on cpDNA rpl16.Chengdu:Sichuan Agricultural University,2014
[19]彭铁成,徐芳,罗静瑶,陈云云,汤华.基于叶绿体基因的香蕉遗传多样性研究.热带生物学报,2012,3(4):293-294Peng T C,Xu F,Luo J Y,Chen Y Y,Tang H.Genetic diversity of banana varieties based on genes from chloroplast.Journal of Tropical Organisms,2012,3(4):293-294
[20]郭会芳,阚显照,张韧,陈鸿姗.nrDNA ITS和cpDNA rpl16基因测序法对六种鼠尾草的检测.植物分类与资源学报,2008,30(3):345-350Guo H F,Kan X Z,Zhang R,Chen H S.Identification of SalviaspeciesbynrDNAITSandcpDNArpl16sequenceanalyses.Plant Diversity and Resources,2008,30(3):345-350
[21]Jordan WC,CourtneyM W,NeigelJE.Lowlevelsof intraspecific genetic variation at a rapidly evolving chloroplast DNAlocusinnorth Americanduckweeds(Lemnaceae).American Journal of Botany,1996,83:430-439
[22]Doyle J J,Doyle J L.A rapid DNA isolation procedure for small quantities of fresh leaf tissue.Phytochemical Bulletin,1987,19(1):11-15
[23]Hall T A.BioEdit:a user friendly biological sequence alignment editorandanalysisprogramfor Windows95/98/NT.Nucleic Acids Symposium Series,1999,41:95-98
[24]Thompson J D,Gibson T J,Plewniak F,Higgins D G.The ClustalXwindowsinterface:flexiblestrategiesformultiple sequencealignmentaidedbyqualityanalysistools.Nucleic Acids Research,1997,25(24):4876-4882
[25]RaymondM,PoussetF.Anexacttestforpopulation differentiation.Evolution,1995,49(6):1280-1283
[26]赵玉倩,丁瑞华,李玉帆,刘正权,贾桂霞.中国特有植物南川百合群体遗传多样性研究.分子植物育种,2016,14(3):749-755Zhao YQ,DingRH,Li YF,LiuZQ,JiaGX.Studieson geneticdiversityofLiliumrosthorniiDielspopulations,the endemicplantsofChina.MolecularPlantBreeding,2016,14(3):749-755
[27]Tajima F.Statistical method for testing the neutral mutation hypothesis by DNA polymorphism.Genetics,1989,123(3):585-595
[28]Fu Y X,Li W H.Statistical tests of neutrality of mutations.Genetics,1993,133(3):693-709
[29]Excoffier L,Laval G,Schneider S.Arlequin(version 3):an integrated software package for population genetics data analysis.Evolutionary Bioinformatics Online,2005,1(4A):47-50
[30]张成林,郭智慧,张新全,白且史,张昌平,李平,马啸.利用SSR标记对垂穗披碱草和老芒麦进行物种鉴定和遗传变异分析.植物遗传资源学报,2016,17(3):416-422ZhangCL,GuoZH,ZhangXQ,BaiQS,ZhangCP,LiP,MaX.Ananalysisofspeciesidentificationandgenetic divergence of drooping wildrye(Elymus nutans)and Siberian wildrye(E.sibiricus)by SSR markers.Journal of P1ant Genetic Resources,2016,17(3):416-422
[31]Brown A H D,Clegg M T,Kahler A L.Plant population genetics,breeding and genetics resources.Bioscience,1990,41(1):613-614
[32]Loveless M D,Hamrick J L.Ecological determinants of genetic structure in plant populations.Annual Review of Ecology and Systematics,1984,15:65-95
[33]Petit R J,Duminil J,Fineschi S.Compative organization ofchloroplast,mitochondrialandnucleardiversityinplant populations.Molecular Ecology,2005,14(3):689-701
[34]刘春香,李钧敏,李红丽,罗芳丽.匍匐茎克隆植物蛇莓的遗传多样性和遗传结构.江苏农业科学,2016,44(2):215-220Liu C X,Li J M,Li H L,Luo F L.Genetic diversity and genetic structure of creeping stem clone plant mock-strawberry.Jiangsu Agricultural Sciences,2016,44(2):215-220
[35]陈俊秋,慈秀芹,李巧明,李捷.樟科濒危植物思茅木姜子遗传多样性的ISSR分析.生物多样性,2006,14(5):410-420ChenJQ,CiXQ,LiQM,LiJ.GeneticdiversityofLitsea szemaois,anendangeredspeciesendemictoChina,detected by inter-simple sequence repeat(ISSR).Biodiversity Science,2006,14(5):410-420
[36]关文灵,李叶芳,陈贤.大百合自然繁殖特性研究.园艺学报,2009,36(S):2056Guan W L,Li Y F,Chen X.Study on the natural propagation characteristics of Cardiocrinum giganteum.Acta Horticulturae Sinica,2009,36(S):2056
[37]Buso G S C,Rangel P H,Ferreira M E.Analysis of genetic variabilityofSouth Americanwildfleepopulations(Oryza glumaepatula)with isozymes and RAPD markers.Molecular Ecology,1998,7(1):107-117
[38]Bussell J D.The distribution of random amplified polymorphic DNA(RAPD)diversity amongst populations of Isotoma perraea(Lobeliaceae).Molecular Ecology,1999,8(5):775-789
[39]段青,崔光芬,王祥宁,王继华,贾文杰,马璐琳,吴丽芳.云南泸定百合遗传多样性的表型与ISSR分析.西北植物学报,2013,33(6):1106-1113Duan Q,Cui G F,Wang X N,Wang J H,Jia W J,Ma L L,Wu L F.Genetic diversity of Lilium sargentiae based on phenotypic traitsandSSRmarkers.ActaBotanicaBoreali-Occidentalia Sinica,2013,33(6):1106-1113
[40]吴祝华.百合属部分种亲缘关系与岷江百合群体遗传结构研究.南京:南京农业大学,2008Wu Z H.Research on genetic relationship conservation genetics of Lilium species and of L.regale.Nanjing:Nanjing Agricultural University,2008
[41]熊勇,陈名红,熊华斌.百合psb A-trn H基因序列变异及遗传多样性分析.生物技术,2013,23(2):53-56Xiong Y,ChenMH,XiongHB.Geneticdiversityanalysis ofLiliumbasedonpsb A-trn Hgenesequencevariation.Biotechnology,2013,23(2):53-56
[42]Viglant L,Stoneking M,Harpending H,Hawkes K,Wilson A C.African populations and the evolution of human mitoehondrial DNA. Science,1991,253(5027):1503-1507
[43]伍丹.四川大百合繁殖及遗传多样性研究.成都:四川农业大学,2006WuD.Studyonreproductionandgeneticdiversityof CardiocrinumgiganteuminSichuan.Chengdu:Sichuan Agricultural University,2006