2016年德宏州新报告HIV/HCV合并感染者中HCV基因型分布特征
|
摘要
目的了解2016年云南省德宏傣族景颇族自治州(德宏州)新报告艾滋病病毒(HIV)感染者合并丙型肝炎病毒(HCV)感染人群的HCV基因型分布特征。方法选取HIV/HCV合并感染者血浆量为200μL以上的样本,经聚合酶链式反应(PCR)扩增HCV核心蛋白结合包膜蛋白(Core/E1)基因与非结构蛋白(NS5B)基因区并测序,通过HCV序列数据库的HCV blast及Mega 7.0软件构建分子系统进化树确定HCV基因型。并按照不同人口学特征对HIV/HCV合并感染者的HCV基因亚型流行特点进行统计学分析。结果 336例HIV/HCV合并感染者中,血浆量>200μL的样本318例,其中有267例(84.0%)成功扩增HCV基因片段并分型,中国籍51例(19.1%),缅甸籍216例(80.9%)。基因分型结果显示:中国籍和缅甸籍的感染者HCV基因亚型均以3b为主,分别占39.2%(20/51)、31.0%(67/216);6n与6u基因亚型次之,在中国籍中分别占15.7%(8/51)、19.6%(10/51),在缅甸籍中分别占19.0%(41/216)、14.8%(32/216)。中国籍感染者的HCV亚型在性别、职业分布上差异有统计学意义(P<0.05);缅甸籍的感染者HCV亚型仅在文化程度分布上差异有统计学意义(P<0.05)。结论 2016年德宏州新报告的HIV感染者合并HCV感染人群的HCV基因亚型复杂多样,中国籍和缅甸籍感染者的HCV基因亚型分布特征一致,均以3b亚型为主要流行优势株,6n、6u次之。
Objective To investigate HCV genotype distribution among newly reported HIV/HCV co-infected patients in Dehong prefecture in 2016. Methods Among 336 HIV/HCV co-infected patients, 318 samples with a plasma volume of 200μl were selected. The HCV core protein-binding protein(Core/E1) gene and non-structural protein(NS5B) gene regions were amplified by PCR. Using HCV BLAST program(HCV database), gene sequences obtained were compared with sequences deposited in HCV database. The molecular phylogenetic tree was constructed and the HCV genotype was identified. The prevalence of HCV genotypes was analyzed in HIV/HCV co-infected patients according to different socio-demographic characteristics. Results 267 HCV sequences were obtained(the overall success rate of amplification was 84.0%, 267/318). 51(19.1%) of them were Chinese and 216(80.9%) Burmese. We found that HCV 3b subtype were predominant, both in Chinese(39.2%, 20/51) and in Burmese(31.0%, 67/216), closely followed by subtypes 6n and 6u. The HCV genotypes were statistically significant in the distribution of gender and occupation in Chinese infected people(P<0.05), and only statistically significant in the distribution of education in Burma infected people(P<0.05). Conclusion In 2016, the prevalence of HCV genotypes among Chinese and Burmese HIV/HCV co-infected individuals are complex and diverse among the newly-reported HIV population in Dehong prefecture. HCV subtype 3b is predominant among Chinese and Burmese, and followed by 6n and 6u. There is no significant difference in the distribution of HCV genotypes between Chinese and Burmese.
Objective To investigate HCV genotype distribution among newly reported HIV/HCV co-infected patients in Dehong prefecture in 2016. Methods Among 336 HIV/HCV co-infected patients, 318 samples with a plasma volume of 200μl were selected. The HCV core protein-binding protein(Core/E1) gene and non-structural protein(NS5B) gene regions were amplified by PCR. Using HCV BLAST program(HCV database), gene sequences obtained were compared with sequences deposited in HCV database. The molecular phylogenetic tree was constructed and the HCV genotype was identified. The prevalence of HCV genotypes was analyzed in HIV/HCV co-infected patients according to different socio-demographic characteristics. Results 267 HCV sequences were obtained(the overall success rate of amplification was 84.0%, 267/318). 51(19.1%) of them were Chinese and 216(80.9%) Burmese. We found that HCV 3b subtype were predominant, both in Chinese(39.2%, 20/51) and in Burmese(31.0%, 67/216), closely followed by subtypes 6n and 6u. The HCV genotypes were statistically significant in the distribution of gender and occupation in Chinese infected people(P<0.05), and only statistically significant in the distribution of education in Burma infected people(P<0.05). Conclusion In 2016, the prevalence of HCV genotypes among Chinese and Burmese HIV/HCV co-infected individuals are complex and diverse among the newly-reported HIV population in Dehong prefecture. HCV subtype 3b is predominant among Chinese and Burmese, and followed by 6n and 6u. There is no significant difference in the distribution of HCV genotypes between Chinese and Burmese.
引文
[1] Bezerra LMD.Global Report:UNAIDS Report on the Global AIDS Epidemic:2010[J].Geneva Switzerland UNAIDS,2012,27(7):553-556.
[2] Gower E,Estes C,Blach S,et al.Global epidemiology and genotype distribution of the hepatitis C virus infection[J].Journal of Hepatology,2014,61(1):S45-S57.
[3] Aceijas C,Rhodes T.Global estimates of prevalence of HCV infection among injecting drug users[J].International Journal of Drug Policy,2007,18(5):352-358.
[4] Hajarizadeh B,Grebely J,Dore GJ.Epidemiology and natural history of HCV infection[J].Nature Reviews Gastroenterology & Hepatology,2013,10(9):553-562.
[5] Martín-Carbonero Luz,Soriano V,Valencia E,et al.Increasing Impact of Chronic Viral Hepatitis on Hospital Admissions and Mortality among HIV-Infected Patients[J].AIDS Research and Human Retroviruses,2001,17(16):1467-1471.
[6] Torriani FJ,RM Ribeiro,TL Gilbert,et al.Hepatitis C virus (HCV) and human immunodeficiency virus (HIV) dynamics during HCV treatment in HCV/HIV coinfection[J].J Infect Dis,2003,188(10):1498-1507.
[7] Sulkowski MS.Management of acute and chronic HCV infection in persons with HIV coinfection[J].J Hepatol,2014,61(1 Suppl):108-119.
[8] Moise L,Terry F,Gutierrez AH,et al.Smarter vaccine design will circumvent regulatory T cell-mediated evasion in chronic HIV and HCV infection[J].Frontiers in Microbiology,2014,5.(Epub ahead of print).
[9] Chlabicz S,Flisiak R,Kowalczuk O,et al.Changing HCV genotypes distribution in Poland—Relation to source and time of infection[J].Journal of Clinical Virology,2008,42(2):1-159.
[10] Legrandabravanel F,Colson P,Leguillouguillemette H,et al.Influence of the HCV subtype on the virological response to pegylated interferon and ribavirin therapy[J].Journal of Medical Virology,2010,81(12):2029-2035.
[11] Roffi L,Redaelli A,Colloredo G,et al.Outcome of liver disease in a large cohort of histologically proven chronic hepatitis C:influence of HCV genotype[J].European Journal of Gastroenterology & Hepatology,2001,13(5):501-506.
[12] Zhang T,DC Tully,S Zhou,et al.Characteristics of HCV Co-Infection among HIV Infected Individuals from an Area with High Risk of Blood-Borne Infections in Central China[J].PLoS One,2014,9(4):e94219.
[13] 段松,项丽芬,杨跃诚,等.云南省德宏州静脉注射毒品者HIV新发感染率及其危险因素研究[J].中华流行病学杂志,2009,30(12):1226-1229.
[14] 朵林,刘济,高世强,等.中缅静脉吸毒人员HIV、HCV、梅毒感染情况及其影响因素对比分析[J].现代预防医学,2014,41(11):2079-2081.
[15] 陈旭,苏明华,江建宁,等.广西地区HIV合并HCV感染人群HCV基因型分布特征[J].中国艾滋病性病,2016,22(8):593-596.
[16] 彭瑾瑜,贺健梅,郑军,等.湖南省107例HIV和HCV双重感染者HCV基因型分布[J].中华实验和临床病毒学杂志,2017,31(2):127-130.
[17] 培尔顿·米吉提,张跃新,买买提力·吾布力,等.2005—2011年新疆地区HIV/AIDS病人中HCV感染状况及影响因素分析[J].中国艾滋病性病,2015,21(6):477-480.
[18] 蔡卫平,梁志伟,兰芸,等.广东省HIV/HCV合并感染人群HCV基因型流行特点[C].中华医学会第十三次全国感染病学术会议论文汇编,2014.
[19] 张淑琼,汪亚玲,李晓非,等.HIV/HCV合并感染者HCV基因分型特点及流行情况分析[J].国际检验医学杂志,2014,35(7):920-922.
[20] 王译葵,唐仁海,周素娟,等.云南省德宏州美沙酮维持治疗者丙型肝炎病毒基因亚型分布[J].中华传染病杂志,2015,33(3):177-178.
[21] 王译葵,冯凯迪,王继宝,等.云南省德宏傣族景颇族自治州中国与缅甸籍吸毒人员的丙型肝炎病毒基因型分析[J].中华预防医学杂志,2016,50(11):959-965.
[22] 张智辉.云南省静脉注射吸毒人群中HCV感染的分子流行病学研究[D].北京:北京协和医学院,2014.
[23] Hnatyszyn HJ.Chronic hepatitis C and genotyping:the clinical significance of determining HCV genotypes[J].Antivir Ther,2005,10(1):1.
[24] 李强,黄玉仙,陈良.HCV相关肝硬化抗病毒治疗现状[J].临床肝胆病杂志,2015,31(11):1813-1816.
[25] 温先勇,唐敏,邓正华,等.中国西南三地HCV基因型的分布及临床特征[J].中国现代医学杂志,2016,26(23):42-46.
[26] 谢尧,徐道振,陆志檬,等.HCV基因型对慢性丙型肝炎干扰素疗效的影响[J].中华肝脏病杂志,2004,12(2):72-75.
[27] 黄辉红,周元平,杨洁,等.联合分析HCV种属信息区和高度保守区序列确定广东地区HCV基因型和亚型[J].广东医学,2010,31(7):825-828.
[28] 刘丽君,张瑞,李俊强,等.中国东北部延边地区丙型肝炎病毒1a型感染研究[J].中华医学杂志,2007,87(20):1407-1410.
[29] Lai MM.RNA recombination in animal and plant viruses[Z].Micro-biol Rev,1992:61-79.
[30] Characterization of Hepatitis C Virus Recombination in Cameroon by Use of Nonspecific Next-Generation Sequencing[J].J Clin Microbiol,2015,53(10):3155-3164.
[31] 张克英,周林源,杨晓江,等.亚洲地区丙型肝炎病毒基因重组研究[J].兰州大学学报,2014,40(4):13-17.
[32] 张文杰,杜绍财,杨建军,等兰州地区HCV疑似2i/2a基因重组变异病例与干扰素仪应答关系的探讨[J].中华肝脏病杂志,2014,22(7):484-489.
[2] Gower E,Estes C,Blach S,et al.Global epidemiology and genotype distribution of the hepatitis C virus infection[J].Journal of Hepatology,2014,61(1):S45-S57.
[3] Aceijas C,Rhodes T.Global estimates of prevalence of HCV infection among injecting drug users[J].International Journal of Drug Policy,2007,18(5):352-358.
[4] Hajarizadeh B,Grebely J,Dore GJ.Epidemiology and natural history of HCV infection[J].Nature Reviews Gastroenterology & Hepatology,2013,10(9):553-562.
[5] Martín-Carbonero Luz,Soriano V,Valencia E,et al.Increasing Impact of Chronic Viral Hepatitis on Hospital Admissions and Mortality among HIV-Infected Patients[J].AIDS Research and Human Retroviruses,2001,17(16):1467-1471.
[6] Torriani FJ,RM Ribeiro,TL Gilbert,et al.Hepatitis C virus (HCV) and human immunodeficiency virus (HIV) dynamics during HCV treatment in HCV/HIV coinfection[J].J Infect Dis,2003,188(10):1498-1507.
[7] Sulkowski MS.Management of acute and chronic HCV infection in persons with HIV coinfection[J].J Hepatol,2014,61(1 Suppl):108-119.
[8] Moise L,Terry F,Gutierrez AH,et al.Smarter vaccine design will circumvent regulatory T cell-mediated evasion in chronic HIV and HCV infection[J].Frontiers in Microbiology,2014,5.(Epub ahead of print).
[9] Chlabicz S,Flisiak R,Kowalczuk O,et al.Changing HCV genotypes distribution in Poland—Relation to source and time of infection[J].Journal of Clinical Virology,2008,42(2):1-159.
[10] Legrandabravanel F,Colson P,Leguillouguillemette H,et al.Influence of the HCV subtype on the virological response to pegylated interferon and ribavirin therapy[J].Journal of Medical Virology,2010,81(12):2029-2035.
[11] Roffi L,Redaelli A,Colloredo G,et al.Outcome of liver disease in a large cohort of histologically proven chronic hepatitis C:influence of HCV genotype[J].European Journal of Gastroenterology & Hepatology,2001,13(5):501-506.
[12] Zhang T,DC Tully,S Zhou,et al.Characteristics of HCV Co-Infection among HIV Infected Individuals from an Area with High Risk of Blood-Borne Infections in Central China[J].PLoS One,2014,9(4):e94219.
[13] 段松,项丽芬,杨跃诚,等.云南省德宏州静脉注射毒品者HIV新发感染率及其危险因素研究[J].中华流行病学杂志,2009,30(12):1226-1229.
[14] 朵林,刘济,高世强,等.中缅静脉吸毒人员HIV、HCV、梅毒感染情况及其影响因素对比分析[J].现代预防医学,2014,41(11):2079-2081.
[15] 陈旭,苏明华,江建宁,等.广西地区HIV合并HCV感染人群HCV基因型分布特征[J].中国艾滋病性病,2016,22(8):593-596.
[16] 彭瑾瑜,贺健梅,郑军,等.湖南省107例HIV和HCV双重感染者HCV基因型分布[J].中华实验和临床病毒学杂志,2017,31(2):127-130.
[17] 培尔顿·米吉提,张跃新,买买提力·吾布力,等.2005—2011年新疆地区HIV/AIDS病人中HCV感染状况及影响因素分析[J].中国艾滋病性病,2015,21(6):477-480.
[18] 蔡卫平,梁志伟,兰芸,等.广东省HIV/HCV合并感染人群HCV基因型流行特点[C].中华医学会第十三次全国感染病学术会议论文汇编,2014.
[19] 张淑琼,汪亚玲,李晓非,等.HIV/HCV合并感染者HCV基因分型特点及流行情况分析[J].国际检验医学杂志,2014,35(7):920-922.
[20] 王译葵,唐仁海,周素娟,等.云南省德宏州美沙酮维持治疗者丙型肝炎病毒基因亚型分布[J].中华传染病杂志,2015,33(3):177-178.
[21] 王译葵,冯凯迪,王继宝,等.云南省德宏傣族景颇族自治州中国与缅甸籍吸毒人员的丙型肝炎病毒基因型分析[J].中华预防医学杂志,2016,50(11):959-965.
[22] 张智辉.云南省静脉注射吸毒人群中HCV感染的分子流行病学研究[D].北京:北京协和医学院,2014.
[23] Hnatyszyn HJ.Chronic hepatitis C and genotyping:the clinical significance of determining HCV genotypes[J].Antivir Ther,2005,10(1):1.
[24] 李强,黄玉仙,陈良.HCV相关肝硬化抗病毒治疗现状[J].临床肝胆病杂志,2015,31(11):1813-1816.
[25] 温先勇,唐敏,邓正华,等.中国西南三地HCV基因型的分布及临床特征[J].中国现代医学杂志,2016,26(23):42-46.
[26] 谢尧,徐道振,陆志檬,等.HCV基因型对慢性丙型肝炎干扰素疗效的影响[J].中华肝脏病杂志,2004,12(2):72-75.
[27] 黄辉红,周元平,杨洁,等.联合分析HCV种属信息区和高度保守区序列确定广东地区HCV基因型和亚型[J].广东医学,2010,31(7):825-828.
[28] 刘丽君,张瑞,李俊强,等.中国东北部延边地区丙型肝炎病毒1a型感染研究[J].中华医学杂志,2007,87(20):1407-1410.
[29] Lai MM.RNA recombination in animal and plant viruses[Z].Micro-biol Rev,1992:61-79.
[30] Characterization of Hepatitis C Virus Recombination in Cameroon by Use of Nonspecific Next-Generation Sequencing[J].J Clin Microbiol,2015,53(10):3155-3164.
[31] 张克英,周林源,杨晓江,等.亚洲地区丙型肝炎病毒基因重组研究[J].兰州大学学报,2014,40(4):13-17.
[32] 张文杰,杜绍财,杨建军,等兰州地区HCV疑似2i/2a基因重组变异病例与干扰素仪应答关系的探讨[J].中华肝脏病杂志,2014,22(7):484-489.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |