二苯乙烯苷对慢性氟中毒致脑损伤大鼠学习记忆能力、氧化应激的影响
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摘要
目的:探讨二苯乙烯苷对慢性氟中毒致脑损伤大鼠学习记忆能力、氧化应激的影响。方法:30只大鼠随机分为对照组、模型组、实验组,每组大鼠10只。对照组大鼠给予去离子水自由饮用;模型组、实验组大鼠给予100 mg/L Na F溶液自由饮用,连续饮用12周。实验组给予二苯乙烯苷60 mg/(kg·d)灌胃,对照组和模型组给予等剂量生理盐水。比较各组大鼠逃避潜伏期、游泳时间百分比、穿过原平台次数及血浆SOD和MDA含量变化。结果:与模型组比较,实验组大鼠逃避潜伏期缩短(P<0.05);与模型组比较,实验组大鼠游泳时间百分比增加(P<0.05);与模型组比较,实验组大鼠穿过原平台次数增加(P<0.05);与模型组比较,实验组血浆SOD含量明显增加,MDA含量明显降低(P<0.05)。结论:二苯乙烯苷可明显改善慢性氟中毒致脑损伤大鼠学习记忆能力,通过降低MDA含量和提高SOD含量减轻脑损伤。
Objective:To investigate Stilbene Glucoside on chronic fluorosis rats with brain injury learning and memory ability of oxidative stress.Method:A total of 30 rats were randomly divided into the control group,model group and experimental group,10 rats in each group.The control group was given deionized water ad libitum,the model group and the experimental group were given 100 mg/L Na F solution free drink,continuous drinking for 12 weeks.The experimental group was given Stilbene Glycosides 60 mg/(kg·d) gavage,the control group and the model group received normal saline.The escape latency,the percentage of swimming time,through the platform of the original frequency and plasma SOD and MDA content changes of three groups were compared.Result:Compared with the model group,the escape latency of the experimental group was shorten(P<0.05).Compared with the model group,the swimming time percentage of the experimental group was increased(P<0.05).Compared with the model group,through the platform of the original frequency of the experimental group was increased(P<0.05).Compared with the model group,plasma SOD of the experimental group was significantly increased,MDA decreased significantly(P<0.05).Conclusion:Stilbene glycosides can significantly improve the chronic fluorosis induced brain learning and memory in rats,and by reducing the content of MDA and increasing SOD reduce brain damage.
Objective:To investigate Stilbene Glucoside on chronic fluorosis rats with brain injury learning and memory ability of oxidative stress.Method:A total of 30 rats were randomly divided into the control group,model group and experimental group,10 rats in each group.The control group was given deionized water ad libitum,the model group and the experimental group were given 100 mg/L Na F solution free drink,continuous drinking for 12 weeks.The experimental group was given Stilbene Glycosides 60 mg/(kg·d) gavage,the control group and the model group received normal saline.The escape latency,the percentage of swimming time,through the platform of the original frequency and plasma SOD and MDA content changes of three groups were compared.Result:Compared with the model group,the escape latency of the experimental group was shorten(P<0.05).Compared with the model group,the swimming time percentage of the experimental group was increased(P<0.05).Compared with the model group,through the platform of the original frequency of the experimental group was increased(P<0.05).Compared with the model group,plasma SOD of the experimental group was significantly increased,MDA decreased significantly(P<0.05).Conclusion:Stilbene glycosides can significantly improve the chronic fluorosis induced brain learning and memory in rats,and by reducing the content of MDA and increasing SOD reduce brain damage.
引文
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[14]苏艳丽,刘明海,吴昌学,等.慢性氟中毒大鼠学习记忆损害与AKT激酶表达[J].贵阳医学院学报,2014,39(3):294-297.
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[20]Gill R S,Lee T F,Liu J Q,et al.Cyclosporine Treatment Reduces Oxygen Free Radical Generation and Oxidative Stress in the Brain of Hypoxia-Reoxygenated Newborn Piglets[J].PLo SOne,2012,7(7):e40 471.
[21]Song Y,Ji B,Wang D S,et al.Effect of acupuncture at different time points on kidney function and oxygen free radical metabolism in rats with simulated weightlessness[J].Chinese Acupuncture&Moxibustion,2014,34(11):19-24.
[2]官志忠,高勤,桂传枝,等.慢性氟中毒脑损伤机制探讨[J].中华地方病学杂志,2011,30(3):352-354.
[3]Dong Y,Wang Y,Wei N,et al.Expression of muscarinic acetylcholine receptors in the brain of rats with chronic fluorosis[J].Chinese Journal of Endemiology,2015,34(2):84-88.
[4]董阳婷,官志忠.胆碱能毒蕈碱受体的功能及其在慢性氟中毒脑损伤中的作用[J].国外医学·医学地理分册,2014,13(2):97-103.
[5]Lou D D,Guan Z Z,Pei J J.Alterations of apoptosis and expressions of Bax and Bcl-2 in the cerebral cortices of rats with chronic fluorosis[J].Fluoride,2014,47(3):199-207.
[6]Fan B,Yu Y,Zhang Y.PI3K-Akt1 expression and its significance in liver tissues with chronic fluorosis[J].International Journal of Clinical&Experimental Pathology,2015,8(2):1226-1236.
[7]彭鹏,熊国祚.二苯乙烯苷抑制血管内皮功能障碍研究进展[J].现代医药卫生,2014,19(2):222-224.
[8]Wang Y,Dong Y,Wei N,et al.Influence of chronic fluorosis on expression of quinone oxidoreductase-1 and heme oxygenase-1 in rat brains[J].Chinese Journal of Endemiology,2015,34(4):250-253.
[9]Dong Y,Wang Y,Wei N,et al.Expression of muscarinic acetylcholine receptors in the brain of rats with chronic fluorosis[J].Chinese Journal of Endemiology,2015,34(2):84-88.
[10]赵兴裕.二苯乙烯苷对创伤性脑损伤模型小鼠的神经保护作用[J].中国药房,2016,27(13):1804-1807.
[11]曾翼,赵诚,王霏,等.二苯乙烯苷对TGF-β1诱导新生大鼠心肌成纤维细胞增殖和胶原合成的影响及其机制[J].中国药科大学学报,2014,45(3):362-367.
[12]邱志伟,刘艳洁,官志忠.细胞外信号调节蛋白激酶5在慢性氟中毒大鼠脑组织中的表达及其与大鼠记忆力改变的关系[J].中华地方病学杂志,2016,35(2):94-98.
[13]邹艳萍,向晓雪,刘辉,等.慢性氟中毒对大鼠学习记忆能力影响及龙眼参多糖拮抗作用观察[J].中国地方病防治杂志,2015,30(6):518-519.
[14]苏艳丽,刘明海,吴昌学,等.慢性氟中毒大鼠学习记忆损害与AKT激酶表达[J].贵阳医学院学报,2014,39(3):294-297.
[15]董阳婷,王娅,魏娜,等.慢性氟中毒大鼠大脑胆碱能毒蕈碱受体的表达[J].中华地方病学杂志,2015,34(2):84-88.
[16]Koakutsu T,Nomura R,Yamanouchi S,et al.Oxygen free radical and antioxidant status in necrotizing soft tissue infection of the lower extremity;a report of two cases with opposite outcomes[J].Upsala Journal of Medical Sciences,2012,118(1):46-50.
[17]Saikia G,Dwivedi A K,Iyer P K.Development of solution,film and membrane based fluorescent sensor for the detection of fluoride anions from water[J].Anal Methods,2012,10(10):3180-3186.
[18]Ronsein G E,Guidi D B,Benassi J C,et al.Cytoprotective effects of carvedilol against oxygen free radical generation in rat liver[J].Redox Report Communications in Free Radical Research,2013,10(3):131-137.
[19]Ruan H L,Li Z,Guo K Q,et al.Change of oxygen free radical metabolism and free amino acids of patients with hyperthyroidism[J].Journal of Hainan Medical University,2016,22(8):372-379.
[20]Gill R S,Lee T F,Liu J Q,et al.Cyclosporine Treatment Reduces Oxygen Free Radical Generation and Oxidative Stress in the Brain of Hypoxia-Reoxygenated Newborn Piglets[J].PLo SOne,2012,7(7):e40 471.
[21]Song Y,Ji B,Wang D S,et al.Effect of acupuncture at different time points on kidney function and oxygen free radical metabolism in rats with simulated weightlessness[J].Chinese Acupuncture&Moxibustion,2014,34(11):19-24.
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