青刺尖叶中改善大鼠良性前列腺增生的活性组分筛选
|
摘要
筛选青刺尖叶提取物改善大鼠良性前列腺增生(benign prostatic hyperplasia,BPH)的活性组分并探讨其作用机制。采用丙酸睾酮诱导的去势大鼠模型,以非那雄胺和普乐安为阳性对照,评价青刺尖叶提取物(QCJ)及其大孔树脂各洗脱物低、高剂量组(1.8g生药/kg/d,5.4g生药/kg/d)的抗BPH活性。结果显示,QCJ及各组分均能改善大鼠BPH病理状况,其中,大孔树脂5%乙醇洗脱物(Fr.A)可使BPH大鼠血清前列腺酸性磷酸酶(prostatic acid phosphatase,PACP)含量降低22.78%(p<0.01),双氢睾酮(dihydrotestosterone,DHT)含量降低28.01%(p<0.01);40%乙醇洗脱物(Fr.B)可减轻BPH病理组织形态改变,使BPH大鼠前列腺指数降低7.69%(p<0.01),血清PACP含量降低32.91%(p<0.01),DHT含量降低56.59%(p<0.01)。各组分中Fr.B效果最好,能够改善BPH大鼠的前列腺指数增加、上皮细胞增厚等病理症状,其机制可能与抑制PACP和DHT的生成有关。
The active components in the extracts of Prinsepia utilis Royle leaves(QCJ) to combat benign prostatic hyperplasia(BPH) were screened and the underlying mechanisms were studied. The BPH model was built on the castrated rats by subcutaneous injection of testosterone propionate. The anti-BPH activity of QCJ and its derived fractions eluted from the macroporous resin at a low dose(1.8 g/kg/d) and a high-dose(5.4 g/kg/d), were evaluated using a testosterone propionate-induced castrated rat model and finasteride and Pule'an as positive controls. The results showed that QCJ and each fraction could alleviate the pathological changes in BPH rats. Among them, the 5% aqueous ethanol eluate from the macroporous resin(Fr.A) could reduce the contents of serum prostatic acid phosphatase(PACP) and dihydrotestosterone(DHT) concentration by 22.78% and 28.01%(p<0.01) in BPH rats. The 40% ethanol eluate(Fr.B) could significantly alleviate the pathological changes in the BPH tissues and reduced the prostatic index, serum PACP content and DHT content by 7.69%, 32.91% and 56.59%, respectively(p<0.01). Among all the fractions, Fr. B showed the most effective, and can significantly improved the pathological symptoms such as increased prostate index and prostate epithelial cell thickness in BPH rats. The underlying mechanism may be related to the inhibition of PACP and DHT production.
The active components in the extracts of Prinsepia utilis Royle leaves(QCJ) to combat benign prostatic hyperplasia(BPH) were screened and the underlying mechanisms were studied. The BPH model was built on the castrated rats by subcutaneous injection of testosterone propionate. The anti-BPH activity of QCJ and its derived fractions eluted from the macroporous resin at a low dose(1.8 g/kg/d) and a high-dose(5.4 g/kg/d), were evaluated using a testosterone propionate-induced castrated rat model and finasteride and Pule'an as positive controls. The results showed that QCJ and each fraction could alleviate the pathological changes in BPH rats. Among them, the 5% aqueous ethanol eluate from the macroporous resin(Fr.A) could reduce the contents of serum prostatic acid phosphatase(PACP) and dihydrotestosterone(DHT) concentration by 22.78% and 28.01%(p<0.01) in BPH rats. The 40% ethanol eluate(Fr.B) could significantly alleviate the pathological changes in the BPH tissues and reduced the prostatic index, serum PACP content and DHT content by 7.69%, 32.91% and 56.59%, respectively(p<0.01). Among all the fractions, Fr. B showed the most effective, and can significantly improved the pathological symptoms such as increased prostate index and prostate epithelial cell thickness in BPH rats. The underlying mechanism may be related to the inhibition of PACP and DHT production.
引文
[1]兰茂.滇南本草[M].昆明:云南人民出版社,1975LAN Mao.Materia Medica of Yunnan[M].Kunming:People's Publishing House of Yunnan,1975
[2]管晖,李晓波.民族药青刺尖的化学成分及药理作用研究进展[J].天然产物研究与开发,2016,28:155-159GUAN Hui,LI Xiao-bo.Research progress on chemical constituent and pharmacological activity of the ethno drug of Prinsepia utilis Royle[J].Natural Product Research and Development,2016,28:155-159
[3]Yang Y,Liu G,Zhang X Y,et al.Effect of Prinsepiautilis tea on blood lipids in hyperlipidemic mice[J].Science&Technology of Food Industry,2015,36:131-137
[4]金家宏,宋肖洁,摩根·多斯桑托斯.一种青刺尖嫩叶提取物及其制备方法和应用:CN 105982979A[P].2016.http://www.wanfangdata.com.cn/details/detail.do_type=patent&id=CN 201510051830.1
[5]Gupta R,Goyal R,Bhattacharya S,et al.Antioxidative in vitro and antiosteoporotic activities of Prinsepia utilis Royle in female rats[J].European Journal of Integrative Medicine,2015,7(2):157-163
[6]管斌.青刺尖化学成分及抗肿瘤活性研究[D].上海:上海交通大学,2013GUAN Bin.Studies on the chemical constituents of Prinsepia utilis Royle and anti-tumor activity[D].Shanghai:Shanghai Jiao Tong University,2013
[7]左爱华,韦群辉,曾元儿,等.紫外可见分光光度法测定青刺尖总黄酮含量[J].云南中医中药杂志,2008,29(6):43-44ZUO Ai-hua,WEI Qun-hui,ZENG Yuan-er,et al.Determination of total flavonoids in Prinsepia utilis Royle by UV-Vis Spectrophotometry[J].Yunnan Journal of Traditional Chinese Medicine and Materia Medica,2008,29(6):43-44
[8]邵金良,黎其万,刘家富,等.青刺尖中水解氨基酸含量测定分析[J].中国食品添加剂,2007,2(5):148-152SHAO Jin-liang,LI Qi-wan,LIU Jia-fu,et al.Determination and analysis of hydrolyzed amino acids content in Prinsepia utilis Royle[J].China Food Additives,2007,2(5):148-152
[9]李晓波,管晖,彭崇胜,等.青刺尖在抗前列腺增生药物中的应用:CN106177018A[P],2016-08-09.http://www.wanfangdata.com.cn/details/detail.do_type=patent&id=CN 201610645369.7
[10]Wei H,Wu G,Shi D,et al.Total flavan glycoside from Abacopteris penangiana rhizomes and its acid hydrolysate:Characterisation and anti-benign prostatic hyperplasia potential[J].Food Chemistry,2012,134(4):1959-1966
[11]中国中西医结合学会男科专业委员会.良性前列腺增生中西医结合诊疗指南(试行版)[J].中华男科学杂志,2017,23(3):280-285China association of integrative medicine men's professional committee.guidelines for the diagnosis and treatment of benign prostatic hyperplasia with integrated medicine(Trial version)[J].National Journal of Andrology,2017,23(3):280-285
[12]Paterniti I,Campolo M,Cordaro M,et al.Effects of different natural extracts in an experimental model of benign prostatic hyperplasia(BPH)[J].Inflammation Research,2018,67(7):617-626
[13]Chen J,Song H.Protective potential of epigallocatechin-3-gallate against benign prostatic hyperplasia in metabolic syndrome rats[J].Environmental Toxicology&Pharmacology,2016,45:315-320
[14]Nunzio C D,Presicce F,Tubaro A.Inflammatory mediators in the development and progression of benign prostatic hyperplasia[J].Nature Reviews Urology,2016,13(10):613-626
[15]Ling Z,Fan X R,Hui X,et al.Anti-inflammatory and antioxidant effects of kelong-capsule on testosterone-induced benign prostatic hyperplasia in rats[J].Evidence-based Complementary and Alternative Medicine:e CAM,2018,2018(14):5290514
[16]Roberts M J,Hattwell J P N,Chow C W K,et al.Tartrate inhibition of prostatic acid phosphatase improves seminal fluid metabolite stability[J].Metabolomics,2016,12(11):162-176
[17]Hassan M I,Aijaz A,Ahmad F.Structural and functional analysis of human prostatic acid phosphatase[J].Expert Rev Anticancer Ther,2010,10(7):1055-1068
[18]陈其华,欧阳政洁,赵丹,等益肾通癃胶囊对前列腺增生模型小鼠生殖激素水平的影响[J].中华男科学杂志,2016,22(11):1011-1015CHEN Qi-hua,OUYANG Zheng-jie,ZHAO Dan,et al.Effects of Yishen Tonglong capsules on sex hormone levels in mice with benign prostatic hyperplasia[J].National Journal of Andrology,2016,22(11):1011-1015
[19]Pej?i?T,Tosti T,Te?i??,et al.Testosterone and dihydrotestosterone levels in the transition zone correlate with prostate volume[J].Prostate,2017,77(10):1082-1092
[20]Zhang B,Zhang R W,Yin X Q,et al.Inhibitory activities of some traditional Chinese herbs against testosterone5α-reductase and effects of Cacumen platycladi on hair re-growth in testosterone-treated mice[J].Journal of Ethnopharmacology,2016,177:1-9
[21]王安喜,朱晓雨,黄霆,等.葛根联合肉桂颗粒对小鼠前列腺增生作用研究[J].中华男科学杂志,2017,23(4):353-360WANG An-xi,ZHU Xiao-yu,HUANG Ting,et al.Effects of Kudzu root plus cinnamon granules on prostatic hyperplasia in mice[J].National Journal of Andrology,2017,23(4):353-360
[22]Zou Y,Aboshora W,Li J,et al.Protective Effects of Lepidium meyenii(Maca)aqueous extract and lycopene on testosterone propionate-induced prostatic hyperplasia in mice[J].Phytotherapy Research,2017,31(2):1192-1198
[2]管晖,李晓波.民族药青刺尖的化学成分及药理作用研究进展[J].天然产物研究与开发,2016,28:155-159GUAN Hui,LI Xiao-bo.Research progress on chemical constituent and pharmacological activity of the ethno drug of Prinsepia utilis Royle[J].Natural Product Research and Development,2016,28:155-159
[3]Yang Y,Liu G,Zhang X Y,et al.Effect of Prinsepiautilis tea on blood lipids in hyperlipidemic mice[J].Science&Technology of Food Industry,2015,36:131-137
[4]金家宏,宋肖洁,摩根·多斯桑托斯.一种青刺尖嫩叶提取物及其制备方法和应用:CN 105982979A[P].2016.http://www.wanfangdata.com.cn/details/detail.do_type=patent&id=CN 201510051830.1
[5]Gupta R,Goyal R,Bhattacharya S,et al.Antioxidative in vitro and antiosteoporotic activities of Prinsepia utilis Royle in female rats[J].European Journal of Integrative Medicine,2015,7(2):157-163
[6]管斌.青刺尖化学成分及抗肿瘤活性研究[D].上海:上海交通大学,2013GUAN Bin.Studies on the chemical constituents of Prinsepia utilis Royle and anti-tumor activity[D].Shanghai:Shanghai Jiao Tong University,2013
[7]左爱华,韦群辉,曾元儿,等.紫外可见分光光度法测定青刺尖总黄酮含量[J].云南中医中药杂志,2008,29(6):43-44ZUO Ai-hua,WEI Qun-hui,ZENG Yuan-er,et al.Determination of total flavonoids in Prinsepia utilis Royle by UV-Vis Spectrophotometry[J].Yunnan Journal of Traditional Chinese Medicine and Materia Medica,2008,29(6):43-44
[8]邵金良,黎其万,刘家富,等.青刺尖中水解氨基酸含量测定分析[J].中国食品添加剂,2007,2(5):148-152SHAO Jin-liang,LI Qi-wan,LIU Jia-fu,et al.Determination and analysis of hydrolyzed amino acids content in Prinsepia utilis Royle[J].China Food Additives,2007,2(5):148-152
[9]李晓波,管晖,彭崇胜,等.青刺尖在抗前列腺增生药物中的应用:CN106177018A[P],2016-08-09.http://www.wanfangdata.com.cn/details/detail.do_type=patent&id=CN 201610645369.7
[10]Wei H,Wu G,Shi D,et al.Total flavan glycoside from Abacopteris penangiana rhizomes and its acid hydrolysate:Characterisation and anti-benign prostatic hyperplasia potential[J].Food Chemistry,2012,134(4):1959-1966
[11]中国中西医结合学会男科专业委员会.良性前列腺增生中西医结合诊疗指南(试行版)[J].中华男科学杂志,2017,23(3):280-285China association of integrative medicine men's professional committee.guidelines for the diagnosis and treatment of benign prostatic hyperplasia with integrated medicine(Trial version)[J].National Journal of Andrology,2017,23(3):280-285
[12]Paterniti I,Campolo M,Cordaro M,et al.Effects of different natural extracts in an experimental model of benign prostatic hyperplasia(BPH)[J].Inflammation Research,2018,67(7):617-626
[13]Chen J,Song H.Protective potential of epigallocatechin-3-gallate against benign prostatic hyperplasia in metabolic syndrome rats[J].Environmental Toxicology&Pharmacology,2016,45:315-320
[14]Nunzio C D,Presicce F,Tubaro A.Inflammatory mediators in the development and progression of benign prostatic hyperplasia[J].Nature Reviews Urology,2016,13(10):613-626
[15]Ling Z,Fan X R,Hui X,et al.Anti-inflammatory and antioxidant effects of kelong-capsule on testosterone-induced benign prostatic hyperplasia in rats[J].Evidence-based Complementary and Alternative Medicine:e CAM,2018,2018(14):5290514
[16]Roberts M J,Hattwell J P N,Chow C W K,et al.Tartrate inhibition of prostatic acid phosphatase improves seminal fluid metabolite stability[J].Metabolomics,2016,12(11):162-176
[17]Hassan M I,Aijaz A,Ahmad F.Structural and functional analysis of human prostatic acid phosphatase[J].Expert Rev Anticancer Ther,2010,10(7):1055-1068
[18]陈其华,欧阳政洁,赵丹,等益肾通癃胶囊对前列腺增生模型小鼠生殖激素水平的影响[J].中华男科学杂志,2016,22(11):1011-1015CHEN Qi-hua,OUYANG Zheng-jie,ZHAO Dan,et al.Effects of Yishen Tonglong capsules on sex hormone levels in mice with benign prostatic hyperplasia[J].National Journal of Andrology,2016,22(11):1011-1015
[19]Pej?i?T,Tosti T,Te?i??,et al.Testosterone and dihydrotestosterone levels in the transition zone correlate with prostate volume[J].Prostate,2017,77(10):1082-1092
[20]Zhang B,Zhang R W,Yin X Q,et al.Inhibitory activities of some traditional Chinese herbs against testosterone5α-reductase and effects of Cacumen platycladi on hair re-growth in testosterone-treated mice[J].Journal of Ethnopharmacology,2016,177:1-9
[21]王安喜,朱晓雨,黄霆,等.葛根联合肉桂颗粒对小鼠前列腺增生作用研究[J].中华男科学杂志,2017,23(4):353-360WANG An-xi,ZHU Xiao-yu,HUANG Ting,et al.Effects of Kudzu root plus cinnamon granules on prostatic hyperplasia in mice[J].National Journal of Andrology,2017,23(4):353-360
[22]Zou Y,Aboshora W,Li J,et al.Protective Effects of Lepidium meyenii(Maca)aqueous extract and lycopene on testosterone propionate-induced prostatic hyperplasia in mice[J].Phytotherapy Research,2017,31(2):1192-1198
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |