苹果属栽培种楸子的遗传多样性与遗传结构分析
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摘要
利用荧光SSR分子标记,对新收集的苹果属栽培种楸子种质资源进行了遗传多样性和群体结构分析,明确群体内和群体间的遗传多样性和结构,为苹果属植物种质资源的收集保存和砧木育种亲本选择提供参考。利用荧光SSR构建研究材料的指纹数据,主要利用GenAlEx 6.501软件分析遗传多样性,利用POPULATION 1.2软件基于Nei遗传距离构建Neighbour-Joining(NJ)树,并利用STRUCTURE 2.3.4软件进行群体结构分析。结果表明:19对SSR引物共检测出390个多态性等位变异,平均多态性等位基因数为20.526,平均有效等位基因数为9.399,观察杂合度和期望杂合度的平均值分别为0.706和0.868,香农多样性指数为2.446,高于以往研究的苹果属植物的遗传多样性。基于Nei遗传距离的聚类分析,在遗传距离0.9167处155份材料可以分成3个类群,3个类群间的遗传距离较近,并没有完全按来源地划分为相应的类群。群体结构分析将155份材料划分成了2个稳定的群体,群体结构分组与NJ聚类有相似的结果,其中150份材料的Q值均大于0.6,血缘相对单一。
Genetic diversity and population structure analysis was carried out on the newly collected cultivated species Malus prunifolia(Willd.) Borkh.using the fluorescent labelled SSR molecular markers.This work attempted to identify the genetic diversity and population structure,thus providing guidance for future germplasm collection and preservation as well as selecting the parental lines in rootstock breeding.The genetic diversity index,the Neighbour-Joining evolutionary tree and the population structure were analyzed by using GenAlEx 6.501,POPULATION 1.2 and STRUCTURE 2.3.4,respectively.Three-hundred and ninety polymorphic alleles were detected by 19 SSR primers,with an average allele number of 20.526 and an average effective allele number of 9.399.The average values of heterozygosity and expected heterozygosity were 0.706 and 0.868,respectively.The Shannon diversity index of Malus prunifolia(Willd.) Borkh.was 2.446,higher than that of other Malus Mill.species.By cluster analysis using the Nei genetic distance(0.9167),the accessions were assigned to three groups.A close genetic diversity among three groups was observed,and the groups divided by genetic diversity and the geographical collecting sites were weakly associated.By analyzing the population structure,these accessions were divided into two groups.However,the Q value of 150 accessions were higher than 0.6,suggesting a relatively single blood relationship.
Genetic diversity and population structure analysis was carried out on the newly collected cultivated species Malus prunifolia(Willd.) Borkh.using the fluorescent labelled SSR molecular markers.This work attempted to identify the genetic diversity and population structure,thus providing guidance for future germplasm collection and preservation as well as selecting the parental lines in rootstock breeding.The genetic diversity index,the Neighbour-Joining evolutionary tree and the population structure were analyzed by using GenAlEx 6.501,POPULATION 1.2 and STRUCTURE 2.3.4,respectively.Three-hundred and ninety polymorphic alleles were detected by 19 SSR primers,with an average allele number of 20.526 and an average effective allele number of 9.399.The average values of heterozygosity and expected heterozygosity were 0.706 and 0.868,respectively.The Shannon diversity index of Malus prunifolia(Willd.) Borkh.was 2.446,higher than that of other Malus Mill.species.By cluster analysis using the Nei genetic distance(0.9167),the accessions were assigned to three groups.A close genetic diversity among three groups was observed,and the groups divided by genetic diversity and the geographical collecting sites were weakly associated.By analyzing the population structure,these accessions were divided into two groups.However,the Q value of 150 accessions were higher than 0.6,suggesting a relatively single blood relationship.
引文
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