沙棘黄酮的化学成分及药理作用研究进展
摘要
目的:综述沙棘黄酮的化学成分及其药理作用,为其进一步研究和开发提供参考。方法:以"沙棘""沙棘黄酮""沙棘总黄酮""Hippophae rhamnoides L.""Sea buckthorn flavonoids""Total flavonoids of Hippophae rhamnoides L."等为关键词,组合查询2008年1月-2018年9月在中国知网、万方数据、维普网、中国生物医学文献服务系统(SinoMed)和PubMed等数据库中的相关文献,对沙棘黄酮的化学成分及药理作用进行归纳与总结。结果与结论:共检索到相关文献158篇,其中有效文献46篇。沙棘中的黄酮类化学成分含量较高,广泛存在于沙棘的果实、籽和沙棘叶中,主要有异鼠李素、槲皮素、山柰酚、杨梅素、芦丁、儿茶素等黄酮苷元及其苷类(如异鼠李素-3-O-β-D-葡萄糖苷、槲皮素-3-O-β-D-葡萄糖苷、山柰酚-3-O-芸香糖-7-O-鼠李糖苷等)。沙棘黄酮的药理作用包括抗血栓形成、降血脂、抗动脉粥样硬化、降血压、降血糖、增强免疫功能、抗肿瘤、抗菌、抗辐射等。但沙棘黄酮发挥的药理作用是基于单体化学成分还是多种化学成分联合作用目前并不明确,建议今后沙棘黄酮的研究工作可结合疾病发生的特点,多层次、多方位、多靶点深入探讨其作用机制和功效,以便更加有效地利用沙棘黄酮的活性成分。
引文
[1]包图雅,乌仁图雅,宝音仓.沙棘的化学成分研究概况[J].中国民族医药杂志,2014,20(8):72-73.
[2]CHEN C,GAO W,OU-YANG DW,et al.Three new flavonoids,hippophins K-M,from the seed residue of hippophae rhamnoides subsp.sinensis[J].Nat Prod Res,2014,28(1):24-29.
[3]国家药典委员会.中华人民共和国药典:一部[S].2015年版.北京:中国医药科技出版社,2015:184.
[4]田茜,何晨,贺敬霞,等.沙棘总黄酮-聚乙烯吡咯烷酮K30固体分散体的制备、表征及体外溶出研究[J].中国药房,2017,28(1):115-118.
[5]POP RM,SOCACIU C,PINTEA A,et al.UHPLC/PDA-ESI/MS analysis of the main berry and leaf flavonol glycosides from different carpathian hippophae rhamnoides L.varieties[J].Phytochem Anal,2013,24(5):484-492.
[6]CHRISTAKI,E.Hippophae rhamnoides L.(sea buckthorn):a otential source of nutraceuticals[J].Food and Public Health,2012,2(3):69-72.
[7]樊旭,周鸿立.沙棘黄酮含量的测定方法及其化学成分的研究进展[J].北方园艺,2018,42(6):144-148.
[8]惠人杰,何旭,刘静怡,等.RP-HPLC-DAD法快速测定沙棘叶中6种黄酮成分[J].中国药科大学学报,2017,48(6):696-700.
[9]惠人杰,冯静,蔺默含,等.多指标-正交试验优化沙棘叶和沙棘籽中黄酮的提取工艺[J].中国药房,2017,28(34):4856-4859.
[10]GUTZEIT D,WRAY V,WINTERHALTER P,et al.Preparative isolation and purification of flavonoids and protocatechuic acid from sea buckthorn juice concentrate(hippophae rhamnoides L.ssp.rhamnoides)by high-speedcounter-current chromatography[J].Chromatographia,2007,65(1/2):1-7.
[11]秦振娴,张瑜,齐梦蝶,等.中国沙棘和西藏沙棘叶中化学成分的UPLC/Q-TOF-MS快速分析[J].中国中药杂志,2016,41(8):1461-1468.
[12]MA XY,LAAKSONEN O,ZHENG J,et al.Flavonol glycosides in berries of two major subspecies of sea buckthorn(hippophae rhamnoides L.)and influence of growth sites[J].Food Chem,2016.DOI:10.1016/j.foodchem.2016.01.036.
[13]CHEN C,XU XM,CHEN Y,et al.Identification,quantification and antioxidant activity of acylated flavonol glycosides from sea buckthorn(hippophae rhamnoides ssp.sinensis[J].Food Chem,2013,141(3):1573-1579.
[14]FANG R,VEITCH NC,KITE GC,et al.Enhanced profiling of flavonol glycosides in the fruits of sea buckthorn(hippophae rhamnoides)[J].J Agric Food Chem,2013,61(16):3868-3875.
[15]ZHOU W,YUAN Z,LI G,et al.Isolation and structure determination of a new flavone glycoside from seed residues of seabuckthorn(hippophae rhamnoides L.)[J].Nat Prod Res,2018,32(8):892-897.
[16]李路平,岳海涛,李天舒,等.沙棘提取物对急性血瘀模型大鼠血液流变学及血栓形成的影响[J].中草药,2010,41(2):272-274.
[17]袁本香,杨桂珍,任艳艳.沙棘总黄酮抗角叉菜胶诱发大鼠血栓形成作用的研究[J].时珍国医国药,2008,19(8):1879-1880.
[18]程嘉艺,滕丹,李伟,等.沙棘总黄酮对血管内皮细胞保护作用及机制研究[J].中国中西医结合杂志,2011,31(3):355-358.
[19]刘兵,苑博,宁天一,等.沙棘总黄酮对过氧化氢损伤人血管内皮细胞的保护作用[J].中华中医药学刊,2017,35(8):2158-2160.
[20]YANG X,WANG Q,PANG ZR,et al.Flavonoid-enriched extract from hippophae rhamnoides seed reduces high fat diet induced obesity,hypertriglyceridemia,and hepatic triglyceride accumulation in C57BL/6 mice[J].Pharm Biol,2017,55(1):1207-1214.
[21]WANG J,ZHANG W,ZHU D,et al.Hypolipidaemic and hypoglycaemic effects of total flavonoids from seed residues of hippophae rhamnoides L.in mice fed a high-fat diet[J].J Sci Food Agric,2011,91(8):1446-1451.
[22]孙琛,冯野,谢培.沙棘果渣总黄酮的降血脂及降血糖作用[J].世界中医药,2018,13(1):142-145.
[23]CHEN JH,LEE MS,WANG CP,et al.Autophagic effects of hibiscus sabdariffa leaf polyphenols and epicatechin gallate(ECG)against oxidized LDL-induced injury of human endothelial cells[J].Eur J Nutr,2017,56(5):1963-1981.
[24]张启梅,王颖超.沙棘黄酮调控动脉粥样硬化大鼠自噬相关蛋白的表达[J].云南中医学院学报,2014,37(4):13-16.
[25]LUO Y,SUN G,DONG X,et al.Isorhamnetin attenuates atherosclerosis by inhibiting macrophage apoptosis via PI3K/AKT activation and HO-1 induction[J].PLoS One,2015,10(3):e0120259.
[26]PANG XF,ZHAO JJ,ZHANG W,et al.Antihypertensive effect of total flavones extracted from seed residuesof hippophae rhamnoides L.in sucrose-fed rats[J].Journal of Ethnopharmacolog,2008,117(2):325-331.
[27]何军,陈仪,肖恒怡,等.醋柳黄酮对自发性高血压大鼠主动脉内MCP-1表达的影响[J].四川大学学报(医学版),2009,40(3):481-485..
[28]WANG LM,GAO P,ZHANG M,et al.Prevalence and ethnic pattern of diabetes and prediabetes in China in 2013[J].JAMA,2017,317(24):2515-2523.
[29]孙国志,赵伟.沙棘总黄酮对糖尿病大鼠心肌细胞的影响[J].黑龙江医药科学,2010,33(3):51-52.
[30]伊娟娟,王振宇,白海娜,等.大果沙棘黄酮对四氧嘧啶所致糖尿病小鼠血糖水平的影响[J].食品工业科技,2014,35(6):347-350.
[31]赵波,向晓玲,王微,等.沙棘黄酮制备及体外抑制人前列腺癌PC-3细胞作用研究[J].天然产物研究与开发,2018,30(1):27-32.
[32]GUO R,GUO X,LI T,et al.Comparative assessment of phytochemical profiles,antioxidant and antiproliferative activities of sea buckthorn(hippophae rhamnoides L.)berries[J].Food Chem,2017.DOI:10.1016/j.foodchem.2016.11.063.
[33]张吉科,林美珍,张小民.沙棘不同器官提取物对小鼠实体瘤的抗癌药效学研究[J].国际沙棘研究与开发,2012,10(3):1-6.
[34]LI C,YANG X,CHEN C,et al.Isorhamnetin suppresses colon cancer cell growth through the PI3K-Akt-mTORpathway[J].Mol Med Rep,2014,9(3):935-940.
[35]周吉银,刘莹,周世文.沙棘总黄酮抗心血管疾病和代谢综合征作用研究进展[J].中药药理与临床,2012,28(6):152-155.
[36]冯欣欣,于文会,柏慧敏,等.沙棘黄酮抗衰老作用及对大鼠非特异性免疫功能的影响研究[J].中兽医医药杂志,2015,34(5):5-9.
[37]MISHRA KP,CHANDA S,KARAN D,et al.Effect of seabuckthorn(hippophae rhamnoides L.)flavone on immune system:an in vitro approach[J].Phytother Res,2008,22(11):1490-1495.
[38]焦岩,常影,余世锋,等.大果沙棘总黄酮体外抗氧化和抑菌作用研究[J].食品研究与开发,2015,36(19):12-15.
[39]张立明,杨凤琴,袁本香,等.沙棘总黄酮对4种念珠菌的体外抑菌作用[J].中国医院药学杂志,2010,30(16):1355-1357.
[40]UPADHYAY NK,KUMAR MS,GUPTA A.Antioxidant,cytoprotective and antibacterial effects of sea buckthorn(hippophae rhamnoides L.)leaves[J].Food Chem Toxicol,2010,48(12):3443-3448.
[41]徐敏.沙棘总黄酮防紫外线损伤的实验研究[J].重庆医学,2015,44(33):4618-4620.
[42]宋宛珊,李霖,岳少乾,等.高血压在血管性痴呆病理过程的作用研究进展[J].中国老年学杂志,2017,37(22):5707-5709.
[43]乔晓鸣,任世存.血管性痴呆模型大鼠接受沙棘总黄酮后血清肿瘤坏死因子α及白细胞介素1β的表达[J].中国组织工程研究,2014,18(36):5819-5824.
[44]乔晓鸣,任世存.沙棘总黄酮对血管性痴呆模型大鼠行为学和血液流变学的影响[J].青海医学院学报,2012,33(2):127-130.
[45]樊旭,周鸿立.响应面法优化沙棘黄酮的提取和抗氧化活性的研究[J].河南工业大学学报(自然科学版),2018,39(4):33-38.
[46]KUMAR MY,DUTTA R,PRASAD D,et al.Subcritical water extraction of antioxidant compounds from sea buckthorn(hippophae rhamnoides L.)leaves for the comparative evaluation of antioxidant activity[J].Food Chem,2011,127(3):1309-1316.
[2]CHEN C,GAO W,OU-YANG DW,et al.Three new flavonoids,hippophins K-M,from the seed residue of hippophae rhamnoides subsp.sinensis[J].Nat Prod Res,2014,28(1):24-29.
[3]国家药典委员会.中华人民共和国药典:一部[S].2015年版.北京:中国医药科技出版社,2015:184.
[4]田茜,何晨,贺敬霞,等.沙棘总黄酮-聚乙烯吡咯烷酮K30固体分散体的制备、表征及体外溶出研究[J].中国药房,2017,28(1):115-118.
[5]POP RM,SOCACIU C,PINTEA A,et al.UHPLC/PDA-ESI/MS analysis of the main berry and leaf flavonol glycosides from different carpathian hippophae rhamnoides L.varieties[J].Phytochem Anal,2013,24(5):484-492.
[6]CHRISTAKI,E.Hippophae rhamnoides L.(sea buckthorn):a otential source of nutraceuticals[J].Food and Public Health,2012,2(3):69-72.
[7]樊旭,周鸿立.沙棘黄酮含量的测定方法及其化学成分的研究进展[J].北方园艺,2018,42(6):144-148.
[8]惠人杰,何旭,刘静怡,等.RP-HPLC-DAD法快速测定沙棘叶中6种黄酮成分[J].中国药科大学学报,2017,48(6):696-700.
[9]惠人杰,冯静,蔺默含,等.多指标-正交试验优化沙棘叶和沙棘籽中黄酮的提取工艺[J].中国药房,2017,28(34):4856-4859.
[10]GUTZEIT D,WRAY V,WINTERHALTER P,et al.Preparative isolation and purification of flavonoids and protocatechuic acid from sea buckthorn juice concentrate(hippophae rhamnoides L.ssp.rhamnoides)by high-speedcounter-current chromatography[J].Chromatographia,2007,65(1/2):1-7.
[11]秦振娴,张瑜,齐梦蝶,等.中国沙棘和西藏沙棘叶中化学成分的UPLC/Q-TOF-MS快速分析[J].中国中药杂志,2016,41(8):1461-1468.
[12]MA XY,LAAKSONEN O,ZHENG J,et al.Flavonol glycosides in berries of two major subspecies of sea buckthorn(hippophae rhamnoides L.)and influence of growth sites[J].Food Chem,2016.DOI:10.1016/j.foodchem.2016.01.036.
[13]CHEN C,XU XM,CHEN Y,et al.Identification,quantification and antioxidant activity of acylated flavonol glycosides from sea buckthorn(hippophae rhamnoides ssp.sinensis[J].Food Chem,2013,141(3):1573-1579.
[14]FANG R,VEITCH NC,KITE GC,et al.Enhanced profiling of flavonol glycosides in the fruits of sea buckthorn(hippophae rhamnoides)[J].J Agric Food Chem,2013,61(16):3868-3875.
[15]ZHOU W,YUAN Z,LI G,et al.Isolation and structure determination of a new flavone glycoside from seed residues of seabuckthorn(hippophae rhamnoides L.)[J].Nat Prod Res,2018,32(8):892-897.
[16]李路平,岳海涛,李天舒,等.沙棘提取物对急性血瘀模型大鼠血液流变学及血栓形成的影响[J].中草药,2010,41(2):272-274.
[17]袁本香,杨桂珍,任艳艳.沙棘总黄酮抗角叉菜胶诱发大鼠血栓形成作用的研究[J].时珍国医国药,2008,19(8):1879-1880.
[18]程嘉艺,滕丹,李伟,等.沙棘总黄酮对血管内皮细胞保护作用及机制研究[J].中国中西医结合杂志,2011,31(3):355-358.
[19]刘兵,苑博,宁天一,等.沙棘总黄酮对过氧化氢损伤人血管内皮细胞的保护作用[J].中华中医药学刊,2017,35(8):2158-2160.
[20]YANG X,WANG Q,PANG ZR,et al.Flavonoid-enriched extract from hippophae rhamnoides seed reduces high fat diet induced obesity,hypertriglyceridemia,and hepatic triglyceride accumulation in C57BL/6 mice[J].Pharm Biol,2017,55(1):1207-1214.
[21]WANG J,ZHANG W,ZHU D,et al.Hypolipidaemic and hypoglycaemic effects of total flavonoids from seed residues of hippophae rhamnoides L.in mice fed a high-fat diet[J].J Sci Food Agric,2011,91(8):1446-1451.
[22]孙琛,冯野,谢培.沙棘果渣总黄酮的降血脂及降血糖作用[J].世界中医药,2018,13(1):142-145.
[23]CHEN JH,LEE MS,WANG CP,et al.Autophagic effects of hibiscus sabdariffa leaf polyphenols and epicatechin gallate(ECG)against oxidized LDL-induced injury of human endothelial cells[J].Eur J Nutr,2017,56(5):1963-1981.
[24]张启梅,王颖超.沙棘黄酮调控动脉粥样硬化大鼠自噬相关蛋白的表达[J].云南中医学院学报,2014,37(4):13-16.
[25]LUO Y,SUN G,DONG X,et al.Isorhamnetin attenuates atherosclerosis by inhibiting macrophage apoptosis via PI3K/AKT activation and HO-1 induction[J].PLoS One,2015,10(3):e0120259.
[26]PANG XF,ZHAO JJ,ZHANG W,et al.Antihypertensive effect of total flavones extracted from seed residuesof hippophae rhamnoides L.in sucrose-fed rats[J].Journal of Ethnopharmacolog,2008,117(2):325-331.
[27]何军,陈仪,肖恒怡,等.醋柳黄酮对自发性高血压大鼠主动脉内MCP-1表达的影响[J].四川大学学报(医学版),2009,40(3):481-485..
[28]WANG LM,GAO P,ZHANG M,et al.Prevalence and ethnic pattern of diabetes and prediabetes in China in 2013[J].JAMA,2017,317(24):2515-2523.
[29]孙国志,赵伟.沙棘总黄酮对糖尿病大鼠心肌细胞的影响[J].黑龙江医药科学,2010,33(3):51-52.
[30]伊娟娟,王振宇,白海娜,等.大果沙棘黄酮对四氧嘧啶所致糖尿病小鼠血糖水平的影响[J].食品工业科技,2014,35(6):347-350.
[31]赵波,向晓玲,王微,等.沙棘黄酮制备及体外抑制人前列腺癌PC-3细胞作用研究[J].天然产物研究与开发,2018,30(1):27-32.
[32]GUO R,GUO X,LI T,et al.Comparative assessment of phytochemical profiles,antioxidant and antiproliferative activities of sea buckthorn(hippophae rhamnoides L.)berries[J].Food Chem,2017.DOI:10.1016/j.foodchem.2016.11.063.
[33]张吉科,林美珍,张小民.沙棘不同器官提取物对小鼠实体瘤的抗癌药效学研究[J].国际沙棘研究与开发,2012,10(3):1-6.
[34]LI C,YANG X,CHEN C,et al.Isorhamnetin suppresses colon cancer cell growth through the PI3K-Akt-mTORpathway[J].Mol Med Rep,2014,9(3):935-940.
[35]周吉银,刘莹,周世文.沙棘总黄酮抗心血管疾病和代谢综合征作用研究进展[J].中药药理与临床,2012,28(6):152-155.
[36]冯欣欣,于文会,柏慧敏,等.沙棘黄酮抗衰老作用及对大鼠非特异性免疫功能的影响研究[J].中兽医医药杂志,2015,34(5):5-9.
[37]MISHRA KP,CHANDA S,KARAN D,et al.Effect of seabuckthorn(hippophae rhamnoides L.)flavone on immune system:an in vitro approach[J].Phytother Res,2008,22(11):1490-1495.
[38]焦岩,常影,余世锋,等.大果沙棘总黄酮体外抗氧化和抑菌作用研究[J].食品研究与开发,2015,36(19):12-15.
[39]张立明,杨凤琴,袁本香,等.沙棘总黄酮对4种念珠菌的体外抑菌作用[J].中国医院药学杂志,2010,30(16):1355-1357.
[40]UPADHYAY NK,KUMAR MS,GUPTA A.Antioxidant,cytoprotective and antibacterial effects of sea buckthorn(hippophae rhamnoides L.)leaves[J].Food Chem Toxicol,2010,48(12):3443-3448.
[41]徐敏.沙棘总黄酮防紫外线损伤的实验研究[J].重庆医学,2015,44(33):4618-4620.
[42]宋宛珊,李霖,岳少乾,等.高血压在血管性痴呆病理过程的作用研究进展[J].中国老年学杂志,2017,37(22):5707-5709.
[43]乔晓鸣,任世存.血管性痴呆模型大鼠接受沙棘总黄酮后血清肿瘤坏死因子α及白细胞介素1β的表达[J].中国组织工程研究,2014,18(36):5819-5824.
[44]乔晓鸣,任世存.沙棘总黄酮对血管性痴呆模型大鼠行为学和血液流变学的影响[J].青海医学院学报,2012,33(2):127-130.
[45]樊旭,周鸿立.响应面法优化沙棘黄酮的提取和抗氧化活性的研究[J].河南工业大学学报(自然科学版),2018,39(4):33-38.
[46]KUMAR MY,DUTTA R,PRASAD D,et al.Subcritical water extraction of antioxidant compounds from sea buckthorn(hippophae rhamnoides L.)leaves for the comparative evaluation of antioxidant activity[J].Food Chem,2011,127(3):1309-1316.