河北省唐山地区人群红细胞A_2B亚型分布情况及其分子机制的研究
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摘要
目的研究河北省唐山地区人群A_2B亚型分布情况及分子机制,探讨输血前开展A_2B亚型检测的临床意义。方法收集2017年11月～2018年3月住院、门诊、急诊及体检的AB型标本,采用抗-A_1试剂筛选A_2B亚型。采用PCR技术扩增A_2B标本ABO基因第6～7外显子并进行Sanger法双向测序分析。结果 535例AB型样本检出12例A_2B表型标本(2.24%)。基因测序显示2例标本基因型为ABO*A2.01/ABO*B.01,6例为ABO*A2.05/ABO*B.01,1例为ABO*BA.02/ABO*O.01.02,3例为ABO*A1.02/ABO*B.01。人群ABO*A2.05等位基因频率为0.56%,ABO*A2.01为0.19%。结论河北省唐山地区人群存在一定比例A_2B亚型,其分子机制存在异质性;输血前开展A_2B亚型检测能提升输血安全性。
Objective To investigate the distribution of A_2B subtype individuals and their molecular characteristic in Tangshan Region of Hebei Province.Methods The individuals with AB phenotype were collected in outpatients,in-patients,emergency department and physical examination department from November 2017 to March 2018.A_2B phenotype was screened with Anti-A1.Then the exon 6 to 7 of ABO in the A_2B phenotype individuals was amplified by polymerase chain reaction and sequenced bidirectionally with Sanger method.Results A_2B phenotypic specimens were found in 12 out of 535 AB phenotype individuals(2.24%).The sequence results were showed that genotypes of 2 individuals were ABO*A2.01/ABO*B.01,6 were ABO*A2.05/ABO*B.01, 1 was ABO*BA.02/ABO*O.01.02, and 3 were ABO*A1.02/ABO*B.01.The frequency of ABO*A2.05 allele was 0.56% and ABO*A2.01 was 0.19% in the Tangshan population.Conclusion There was a certain proportion of A_2B subtypes in Tangshan Region of Hebei Province,and its molecular mechanism was heterogeneous.Testing A_2B subtype before transfusion can improve the safety of blood transfusion.
Objective To investigate the distribution of A_2B subtype individuals and their molecular characteristic in Tangshan Region of Hebei Province.Methods The individuals with AB phenotype were collected in outpatients,in-patients,emergency department and physical examination department from November 2017 to March 2018.A_2B phenotype was screened with Anti-A1.Then the exon 6 to 7 of ABO in the A_2B phenotype individuals was amplified by polymerase chain reaction and sequenced bidirectionally with Sanger method.Results A_2B phenotypic specimens were found in 12 out of 535 AB phenotype individuals(2.24%).The sequence results were showed that genotypes of 2 individuals were ABO*A2.01/ABO*B.01,6 were ABO*A2.05/ABO*B.01, 1 was ABO*BA.02/ABO*O.01.02, and 3 were ABO*A1.02/ABO*B.01.The frequency of ABO*A2.05 allele was 0.56% and ABO*A2.01 was 0.19% in the Tangshan population.Conclusion There was a certain proportion of A_2B subtypes in Tangshan Region of Hebei Province,and its molecular mechanism was heterogeneous.Testing A_2B subtype before transfusion can improve the safety of blood transfusion.
引文
[1] 李双玉,董磊,解金辉,等.一例罕见AB亚型的分子生物学研究分析[J].现代检验医学杂志,2018,33(4):70-72.LI Shuangyu,DONG Lei,XIE Jinhui,et al.Study on the molecular mechanism of a case of rare AB subtype[J].J Mod Lab Med,2018,33(4):70-72.
[2] American Association of Blood Banks.Technical Ma-nual[M].18th Ed.Bethesda:AABB,2014:315.
[3] 韩芳,顾松琴.A2B亚型致配血不合一例报告[J].青海医药杂志,2016,46(3):72-73.HAN Fang,GU Songqin.One case of A2B subtype leads to cross blood mismatch[J].Qinghai Medical Journal,2016,46(3):72-73.
[4] 赵春米.输血前A2/A2B亚型和RH弱D血型检测的意义[J].临床合理用药杂志,2014,7(8A):121-122.ZHAO Chunmi.Significance of A2/A2B subtype and RH weak D blood type test before blood transfusion[J].Chinese Journal of Clinical Rational Drug Use,2014,7(8A):121-122.
[5] 兰炯采,■中桥,陈静娴.输血免疫血液学实验技术[M].北京:人民卫生出版社,2011:24.LAN Jiongcai,YUN Zhongqiao,CHEN Jingxian.Blood transfusion immunological techniques[M].Beijing:People’s Health Publishing House,2011:24.
[6] ZHU Fengrong,TAO Shiheng,XU Xuhua,et al.Distribution of ABO blood group allele and identification of three novel alleles in the Chinese Han population[J].Vox Sang Uinis,2010,98(4):554-559.
[7] GIRIYAN S S,AGRAWAL A,BAJPAI R,et al.A1 and A2 sub-types of blood group ‘A’:a reflection of their prevalence in north karnataka region[J].J Clin Diagn Res,2017,11(5):EC40-EC42.
[8] MATZHOLD E M,DREXLER C,WAGNER T.A novel ABO O allele caused by a large deletion covering two exons of the ABO gene identified in a Caucasian family showing discrepant ABO blood typing results[J].Transfusion,2016,56(11):2739-2743.
[9] YING Yanlin,HONG Xiaozhen,XU Xiannguo,et al.Serological characteristic and molecular basis of A2 subgroup in the Chinese population[J].Transfus Apher Sci,2013,48(1):67-74.
[10] OGASAWARA K,YABE R,UCHIKAWA M,et al.Different alleles cause an imbalance in A2 and A2B phenotypes of the ABO blood group[J].Vax Sanguinis,1998,74(4):242-247
[11] MOLLER M, JOUD M, STORRY J R,et al.Erythrogene:a database for in-depth analysis of the extensive variation in 36 blood group systems in the 1000 Genomes Project[J].Blood Adv,2016,1(3):240-249.
[12] 陈妍,马玲,朱绍汶,等.南京地区汉族人群红细胞A血型亚型分子生物学研究[J].临床血液学杂志,2016,29(10):773-776.CHEN Yan,MA Ling,ZHU Shaowen,et al.Analysis and research on molecular mechanism of A subtype groups in Nanjing Han population[J].J Clin Hematol(China),2016,29(10):773-776.
[13] 杜振军,牟曦光,魏莉,等.中国北方汉族人群A204等位基因的发现及家系调查[J].中国输血杂志,2013,26(3):135-138.DU Zhenjun,MOU Xiguang,WEI Li,et al.Discovery of A204 allele and its family study of Han individuals in northern China[J].Chin J Blood Transfusion,2013,26(3):135-138.
[14] 邓刚.B(A)血型分子机制及红细胞表面抗原表达的研究[J].中国输血杂志,2013,26(9):861-863.DENG Gang.Study on the molecular mechanism of B(A) and antigenexpression of red blood cell surface[J].Chin J Blood Transfusion,2013,26(9):861-863.
[2] American Association of Blood Banks.Technical Ma-nual[M].18th Ed.Bethesda:AABB,2014:315.
[3] 韩芳,顾松琴.A2B亚型致配血不合一例报告[J].青海医药杂志,2016,46(3):72-73.HAN Fang,GU Songqin.One case of A2B subtype leads to cross blood mismatch[J].Qinghai Medical Journal,2016,46(3):72-73.
[4] 赵春米.输血前A2/A2B亚型和RH弱D血型检测的意义[J].临床合理用药杂志,2014,7(8A):121-122.ZHAO Chunmi.Significance of A2/A2B subtype and RH weak D blood type test before blood transfusion[J].Chinese Journal of Clinical Rational Drug Use,2014,7(8A):121-122.
[5] 兰炯采,■中桥,陈静娴.输血免疫血液学实验技术[M].北京:人民卫生出版社,2011:24.LAN Jiongcai,YUN Zhongqiao,CHEN Jingxian.Blood transfusion immunological techniques[M].Beijing:People’s Health Publishing House,2011:24.
[6] ZHU Fengrong,TAO Shiheng,XU Xuhua,et al.Distribution of ABO blood group allele and identification of three novel alleles in the Chinese Han population[J].Vox Sang Uinis,2010,98(4):554-559.
[7] GIRIYAN S S,AGRAWAL A,BAJPAI R,et al.A1 and A2 sub-types of blood group ‘A’:a reflection of their prevalence in north karnataka region[J].J Clin Diagn Res,2017,11(5):EC40-EC42.
[8] MATZHOLD E M,DREXLER C,WAGNER T.A novel ABO O allele caused by a large deletion covering two exons of the ABO gene identified in a Caucasian family showing discrepant ABO blood typing results[J].Transfusion,2016,56(11):2739-2743.
[9] YING Yanlin,HONG Xiaozhen,XU Xiannguo,et al.Serological characteristic and molecular basis of A2 subgroup in the Chinese population[J].Transfus Apher Sci,2013,48(1):67-74.
[10] OGASAWARA K,YABE R,UCHIKAWA M,et al.Different alleles cause an imbalance in A2 and A2B phenotypes of the ABO blood group[J].Vax Sanguinis,1998,74(4):242-247
[11] MOLLER M, JOUD M, STORRY J R,et al.Erythrogene:a database for in-depth analysis of the extensive variation in 36 blood group systems in the 1000 Genomes Project[J].Blood Adv,2016,1(3):240-249.
[12] 陈妍,马玲,朱绍汶,等.南京地区汉族人群红细胞A血型亚型分子生物学研究[J].临床血液学杂志,2016,29(10):773-776.CHEN Yan,MA Ling,ZHU Shaowen,et al.Analysis and research on molecular mechanism of A subtype groups in Nanjing Han population[J].J Clin Hematol(China),2016,29(10):773-776.
[13] 杜振军,牟曦光,魏莉,等.中国北方汉族人群A204等位基因的发现及家系调查[J].中国输血杂志,2013,26(3):135-138.DU Zhenjun,MOU Xiguang,WEI Li,et al.Discovery of A204 allele and its family study of Han individuals in northern China[J].Chin J Blood Transfusion,2013,26(3):135-138.
[14] 邓刚.B(A)血型分子机制及红细胞表面抗原表达的研究[J].中国输血杂志,2013,26(9):861-863.DENG Gang.Study on the molecular mechanism of B(A) and antigenexpression of red blood cell surface[J].Chin J Blood Transfusion,2013,26(9):861-863.
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