健脾清化方对2型糖尿病大鼠肠道免疫细胞Th1/Th2/Th17/Treg的影响

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英文篇名：Effects of Jianpi Qinghua Recipe on Intestinal Immune Cells Th1/Th2/Th17/Treg in Type 2 Diabetes Mellitus Rats 作者：韩煦 ; 姚政 ; 李俊燕 ; 陆灏 英文作者：HAN Xu;YAO Zheng;LI Junyan;LU Hao;Endocrinology Department,Shuguang Hospital Affiliated to Shanghai University of TCM; 关键词：2型糖尿病 ; 胰岛素抵抗 ; 健脾清化方 ; 免疫细胞 英文关键词：type 2 diabetes millitus;;intestinal insulin resistance;;Jianpi Qinghua Recipe;;intestinal immune cells 中文刊名：LNZY 英文刊名：Liaoning Journal of Traditional Chinese Medicine 机构：上海中医药大学附属曙光医院内分泌科; 出版日期：2019-07-18 出版单位：辽宁中医杂志 年：2019 期：v.46;No.506 基金：国家自然科学基金项目(81774235,81403359) 语种：中文; 页：LNZY201907062 页数：4 CN：07 ISSN：21-1128/R 分类号：194-196+232

摘要

目的:研究健脾清化方对2型糖尿病大鼠肠道免疫细胞的影响。方法:采用高脂饮食联合链脲佐菌素(STZ)建立稳定的T2DM大鼠模型,随机分为正常对照组、模型对照组和健脾清化方组,灌胃给药4周,检测大鼠随机及空腹血糖,ELISA法检测空腹胰岛素,流式细胞术检测大鼠肠道免疫细胞Th1、Th2、Th17和Treg比例。结果:与模型对照组比较,健脾清化方降低胰岛素抵抗指数(HOMA-IR),减少小肠组织中免疫细胞Th1、Th2和Th17的细胞比例,增加小肠组织中Treg细胞比例。结论:健脾清化方可改善T2DM大鼠的胰岛素抵抗,其机制可能与调节肠道免疫细胞有关。
        Objective:To explore the effects of Jianpi Qinghua Recipe on intestinal immune cells in type 2 diabetes mellitus(T2DM) rats.Methods:The rat model of T2DM was established by a combination of small dosage of streptozotocin(STZ) injection with high-fat diet.Rats were randomly divided into the control group,model group and Jianpi Qinghua Recipe group.All rats were treated by gavage for 4 weeks.The fasting blood glucose of rats was detected by glucometer instantly.Fasting insulin was detected by ELISA.The proportions of Th1,Th2,Th17 and Treg from rats gut were analyzed by flow cytometry.Results:Compared with the model group,Jiangpi Qinghua Recipe reduced HOMA-IR of T2DM rats,decreased the proportions of Th1,Th2 and Th17 cells and increased the proportion of Treg cells in the small intestine.Conclusion:Jianpi Qinghua Recipe improved insulin resistance in T2DM rats by regulating proportions of Th1,Th2,Th17 and Treg in gut.

引文

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