中医方证代谢组学——中药效应评价的有效途径
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摘要
有效性评价是发现中药药效物质基础、先导化合物和质量标志物的重要前提,因此急需建立一种生物学语言,将中药有效性科学地表达出来,进一步凸显中医药的实用价值。证候和方剂是中医药的重要组成部分,与中药有效性直接相关。我们以证候和方剂为研究对象,建立了科学评价中药有效性的创新方法学体系——中医方证代谢组学。它将中药血清药物化学理论与代谢组学技术有机整合,在解决证候生物标记物的基础上,建立方剂药效生物评价体系,发现并确认中药药效物质基础。该策略为提高中医理论和临床实践的科学价值提供了有力支持。本文概述了中医方证代谢组学的研究策略,利用该方法揭示临床常见中医证候生物标记物及开展相关方剂的有效性评价研究,着重阐述了中药药效物质基础及质量标记物的发现。
Evaluation of the efficacy of traditional Chinese medicines(TCMs) is an important prerequisite for discovering effective substances, lead compounds, and quality markers(Q markers). At present, there is an urgent need to develop a biological language that can act as abridge for the scientific elaboration of the efficacy of TCMs, and to further highlight the significant value of TCM. Chinese medicinal syndromes and formulae are two essential parts of TCM that directly relate to its efficacy. Syndromes and formulae have been taken as the research objects. The serum pharmacochemistry of the TCM approach with metabolomics were integrated to establish an innovative chinmedomics strategy, which is able to explore syndrome biomarkers and evaluate TCM efficacy in order to discover effective substances from TCMs. A great deal of concrete work in chinmedomics has already performed to bridge the gap between Chinese and Western medicine, and to provide a powerful approach to enhance the scientific value of TCM theory and clinical practice. This article summarizes the application of chinmedomics in identifying the candidate biomarkers of a syndrome and revealing the efficacy of the related formula. We also highlight the discovery of lead compounds and Q markers from TCMs.
Evaluation of the efficacy of traditional Chinese medicines(TCMs) is an important prerequisite for discovering effective substances, lead compounds, and quality markers(Q markers). At present, there is an urgent need to develop a biological language that can act as abridge for the scientific elaboration of the efficacy of TCMs, and to further highlight the significant value of TCM. Chinese medicinal syndromes and formulae are two essential parts of TCM that directly relate to its efficacy. Syndromes and formulae have been taken as the research objects. The serum pharmacochemistry of the TCM approach with metabolomics were integrated to establish an innovative chinmedomics strategy, which is able to explore syndrome biomarkers and evaluate TCM efficacy in order to discover effective substances from TCMs. A great deal of concrete work in chinmedomics has already performed to bridge the gap between Chinese and Western medicine, and to provide a powerful approach to enhance the scientific value of TCM theory and clinical practice. This article summarizes the application of chinmedomics in identifying the candidate biomarkers of a syndrome and revealing the efficacy of the related formula. We also highlight the discovery of lead compounds and Q markers from TCMs.
引文
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[3]Hu J,Pang WS,Han J,Zhang K,Zhang JZ,Chen LD.Gualou Guizhi decoction reverses brain damage with cerebral ischemic stroke,multi-component directed multi-target to screen calcium-overload inhibitors using combination of molecular docking and protein-protein docking.J Enzyme Inhib Med Chem 2018;33(1):115-25.
[4]Chen L,Wang X,Liu Y,Di X.Dual-target screening of bioactive components from traditional Chinese medicines by hollow fiber-based ligand fishing combined with liquid chromatography-mass spectrometry.J Pharm Biomed Anal 2017;143:269-76.
[5]Cui L,Cai Y,Cheng W,Liu G,Zhao J,Cao H,et al.A novel,multi-target natural drug candidate,matrine,improves cognitive deficits in Alzheimer’s disease transgenic mice by inhibiting Ab aggregation and blocking the RAGE/Ab axis.Mol Neurobiol 2017;54(3):1939-52.
[6]Cao H,Zhang A,Zhang H,Sun H,Wang X.The application of metabolomics in traditional Chinese medicine opens up a dialogue between Chinese and Western medicine.Phytother Res 2015;29(2):159-66.
[7]Zhang Y,Liu Y,Li Y,Zhao X,Zhuo L,Zhou A,et al.Hierarchical and complex system entropy clustering analysis based validation for traditional Chinese medicine syndrome patterns of chronic atrophic gastritis.J Altern Complement Med.Epub 2018 Mar 22.
[8]Wang X,Zhang A,Sun H.Future perspectives of Chinese medical formulae:chinmedomics as an effector.OMICS 2012;16(7-8):414-21.
[9]Zhang AH,Sun H,Yan GL,Wang P,Han Y,Wang XJ.Chinmedomics:a new strategy for research of traditional Chinese medicine.China J Chin Mater Med2015;40(4):569-76.Chinese.
[10]Liu Q,Zhao HW,Zhang AH,Sun H,Zhao XH,Nan Y,et al.Chinmedomics strategy to discover effective constituents and elucidate action mechanism of Nanshi capsule against kidney-yang deficiency syndrome.China J Chin Mater Med 2016;41(15):2901-14.Chinese.
[11]Wang X,Zhang A,Sun H,Wang P.Systems biology technologies enable personalized traditional Chinese medicine:a systematic review.Am J Chin Med 2012;40(6):1109-22.
[12]Zhang A,Sun H,Yan G,Cheng W,Wang X.Systems biology approach opens door to essence of acupuncture.Complement Ther Med 2013;21(3):253-9.
[13]Zhang ND,Han T,Huang BK,Rahman K,Xu HT,Jiang YP,et al.Traditional Chinese medicine formulas for the treatment of osteoporosis:implication for antiosteoporotic drug discovery.J Ethnopharmacol 2016;189:61-80.
[14]Chen M,Yang F,Yang X,Lai X,Gao Y.Systematic understanding of mechanisms of a Chinese herbal formula in treatment of metabolic syndrome by an integrated pharmacology approach.Int J Mol Sci 2016;17(12):E2114.
[15]Xiang Z,Sun H,Cai X,Chen D,Zheng X.The study on the material basis and the mechanism for anti-renal interstitial fibrosis efficacy of rhubarb through integration of metabonomics and network pharmacology.Mol Biosyst2015;11(4):1067-78.
[16]Li XN,Zhang A,Sun H,Song Y,Zou D,Wang X.Rapid discovery of absorbed constituents and metabolites in rat plasma after the oral administration of Zi Shen Wan using high-throughput UHPLC-MS with a multivariate analysis approach.J Sep Sci 2016;39(24):4700-11.
[17]Chu H,Zhang A,Han Y,Lu S,Kong L,Han J,et al.Metabolomics approach to explore the effects of Kai-Xin-San on Alzheimer’s disease using UPLC/ESI-Q-TOF mass spectrometry.J Chromatogr B Analyt Technol Biomed Life Sci2016;1015-1016:50-61.
[18]Sun H,Chen X,Zhang A,Sakurai T,Jiang J,Wang X.Chromatographic fingerprinting analysis of Zhizhu Wan preparation by high-performance liquid chromatography coupled with photodiode array detector.Pharmacogn Mag2014;10(40):470-6.
[19]Zhang A,Zou D,Yan G,Tan Y,Sun H,Wang X.Identification and characterization of the chemical constituents of Simiao Wan by ultra high performance liquid chromatography with mass spectrometry coupled to an automated multiple data processing method.J Sep Sci 2014;37(14):1742-7.
[20]Wang XJ.Progress and future developing of the serum pharmacochemistry of traditional Chinese medicine.China J Chin Mater Med 2006;31(10):789-92,835.Chinese.
[21]Wang X,Lv H,Sun H,Liu L,Yang B,Sun W,et al.Metabolic urinary profiling of alcohol hepatotoxicity and intervention effects of Yin Chen Hao Tang in rats using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry.J Pharm Biomed Anal 2008;48(4):1161-8.
[22]Zhang A,Yan G,Zhou X,Wang Y,Han Y,Guan Y,et al.High resolution metabolomics technology reveals widespread pathway changes of alcoholic liver disease.Mol Biosyst 2016;12(1):262-73.
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