地震灾区环境中细菌对含氯消毒剂抗性的观察
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摘要
目的了解地震灾区环境中细菌对含氯消毒剂抗性情况,为制定自然灾害消毒策略提供科学依据。方法采用肉汤稀释法,对地震灾区环境中分离的细菌对含氯消毒剂抗力情况进行了测定。结果含氯消毒剂对地震灾区消毒区域水中分离的大肠杆菌的MIC值为0.4~0.8 mg/ml,对灾区非消毒区域分离的大肠杆菌以及标准菌株的MIC值为0.5 mg/ml。含氯消毒剂对地震灾区土壤中分离的蜡样芽孢杆菌芽孢的MIC值为0.8~1.3 mg/ml,与其标准菌株芽孢基本一致。结论地震灾区环境中分离的大肠杆菌和蜡样芽孢杆菌芽孢对含氯消毒剂的抵抗力与标准菌株或标准菌株芽孢基本一致。
Objective To know the resistance of bacteria from earthquake-stricken areas to chlorine-containing disinfectant and to provide a scientific basis for natural disasters policy making.Methods Broth dilution method was used to measure the resistance of bacteria from quake-stricken areas to chlorine-containing disinfectant.Results The minimal inhibitory concentration(MIC) of Escherichia coli from water in disinfected earthquake-stricken areas by chlorine-containing disinfectant was 0.4~0.8 mg/ml,and the MIC of E.coli standard and its reference strain in non-disinfected quake-stricken areas was 0.5 mg/ml.The MIC of Bacillus cereus spores from soil in disinfected quake-stricken areas by chlorine-containing disinfectant was 0.8~1.3 mg/ml,which was nearly the same as its reference strains.Conclusions The resistance of E.coli and Bacillus cereus spores from earthquake-stricken areas to chlorine-containing disinfectant was nearly the same as its reference strain.
Objective To know the resistance of bacteria from earthquake-stricken areas to chlorine-containing disinfectant and to provide a scientific basis for natural disasters policy making.Methods Broth dilution method was used to measure the resistance of bacteria from quake-stricken areas to chlorine-containing disinfectant.Results The minimal inhibitory concentration(MIC) of Escherichia coli from water in disinfected earthquake-stricken areas by chlorine-containing disinfectant was 0.4~0.8 mg/ml,and the MIC of E.coli standard and its reference strain in non-disinfected quake-stricken areas was 0.5 mg/ml.The MIC of Bacillus cereus spores from soil in disinfected quake-stricken areas by chlorine-containing disinfectant was 0.8~1.3 mg/ml,which was nearly the same as its reference strains.Conclusions The resistance of E.coli and Bacillus cereus spores from earthquake-stricken areas to chlorine-containing disinfectant was nearly the same as its reference strain.
引文
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[10]Pumbwe L,Skilbeck C,Wexler H.Induction of multiple antibiot-ic resistance in Bacteroides fragilis by benzene and benzene-de-rived active compounds of commonly used analgesics,antisepticsand cleaning agents[J].J Antimicrobial Chemotherapy.2007,60(6):1288.
[11]谈智,陈越英,徐燕,等.14株医院感染病原菌对对氯间二甲苯酚抗力的观察[J].中国消毒学杂志,2007,24(6):496.
[2]卫生部卫生法制与监督司.生活饮用水标准检验法[S].北京:中华人民共和国卫生部,GB 5750.12-2006.
[3]卫生部卫生法制与监督司.消毒技术规范[S].北京:中华人民共和国卫生部,2002:85-86.
[4]Russell AD,Hammond SA,Morgan JR.Bacterial resistance to an-tiseptics and disinfectants[J].J Hosp Infect,1986,7(3):213.
[5]Russell A D.Bacterial adaptation and resistance to antiseptics,disinfectants and preservatives is not a new phenomenon[J].JHosp Infect,2004,57(2):97.
[6]王国庆.细菌对消毒剂抗性的研究进展[J].中国消毒学杂志,2006,23(2):164.
[7]Retyi I.Resistance of Escherichia coli to some antibiotics and bi-ocides in the intestinal biofilm of mice[J].Acta Microbiol Hung,1991,38(1):33.
[8]Mokgatla RM,Brzel VS,Gouws PA.Isolation of Salmonella re-sistant to hypochlorous acid from a poultry abattoir[J].Lett ApplMicrobiol,1998,27(3):379.
[9]张辉,张利军,贾雷立,等.汶川映秀镇灾后终末消毒效果评估[J].中国消毒学杂志,2009,26(3):290.
[10]Pumbwe L,Skilbeck C,Wexler H.Induction of multiple antibiot-ic resistance in Bacteroides fragilis by benzene and benzene-de-rived active compounds of commonly used analgesics,antisepticsand cleaning agents[J].J Antimicrobial Chemotherapy.2007,60(6):1288.
[11]谈智,陈越英,徐燕,等.14株医院感染病原菌对对氯间二甲苯酚抗力的观察[J].中国消毒学杂志,2007,24(6):496.
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