电凝法制作Balb/c小鼠局灶性脑缺血模型的实验研究
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摘要
目的观察电凝法电凝小鼠大脑中动脉,引起大脑中动脉闭塞,建立小鼠脑缺血模型的可行性。方法采用电凝法直接闭塞大脑中动脉(Middle cerebral artery, MCA)制作成年雄性Balb/c小鼠脑缺血模型(模型组,n=20只);同时,同批次Balb/c小鼠经相同部位开颅,但不电凝闭塞大脑中动脉的小鼠作为假手术组(假手术组,n=20只)。于术后第24 h、72 h采用神经功能缺损评分(modified neurological function severity score, mNSS)评估模型组及假手术组小鼠神经功能损伤情况。结果术后第24 h取模型组Balb/c小鼠损伤区域脑组织切片,行苏木素-伊红染色,镜下显示缺血脑组织间质水肿,同时伴有空腔形成,局部脑组织结构变得疏松,且着色较淡,神经细胞数量明显减少,神经细胞胞体缩小,部分细胞轮廓欠清晰,可以见到不同程度的细胞变性坏死,核固缩,核仁消失现象。模型组在术后第24 h神经功能缺损评分明显低于假手术组(P<0.05),且这种差别可维持到术后第72 h。结论电凝法可以在Balb/c小鼠中成功制作小鼠局灶性脑缺血模型。
Objective To investigate the feasibility of establishment of focal cerebral ischemia model in Balb/c mice by electrocoagulation. Method The model of mice about focal cerebral ischemia was mimicked with direct MCA occlusion. Forty adult male Balb/c mice were ramdomized into experimental group and control group. Twenty-four hours and 72 hours after middle cerebral artery occlusion, the neurological function was evaluated according to the modified neurological function severity score(mNSS). Result At 24 hours, the neurological function scores in the experimental group were decreased compared with control group, the difference could sustain to 72 hours later. Specifically, in the experimental group we can find the interstitial edema, cavitation, partly architecture become rarefaction, the number of nerve cells was decreased, the pigmenting of nerve cells was fairly tasteless, and the cell degeneration, karyopyknosis, nucleoli abolition could be found in ischemia brain tissues. Conclusion Modified electrocoagulation method mimicked with direct MCA occlusion can help us establish focal cerebral ischemia model in experimental research.
Objective To investigate the feasibility of establishment of focal cerebral ischemia model in Balb/c mice by electrocoagulation. Method The model of mice about focal cerebral ischemia was mimicked with direct MCA occlusion. Forty adult male Balb/c mice were ramdomized into experimental group and control group. Twenty-four hours and 72 hours after middle cerebral artery occlusion, the neurological function was evaluated according to the modified neurological function severity score(mNSS). Result At 24 hours, the neurological function scores in the experimental group were decreased compared with control group, the difference could sustain to 72 hours later. Specifically, in the experimental group we can find the interstitial edema, cavitation, partly architecture become rarefaction, the number of nerve cells was decreased, the pigmenting of nerve cells was fairly tasteless, and the cell degeneration, karyopyknosis, nucleoli abolition could be found in ischemia brain tissues. Conclusion Modified electrocoagulation method mimicked with direct MCA occlusion can help us establish focal cerebral ischemia model in experimental research.
引文
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[12] 翟小虎,王斌,宋亚刚,等.线栓法制备大鼠脑缺血模型的研究进展与思路[J].实验动物科学,2015,32(5):55-58.
[13] Anderson M F,Sims N R.Mitochondrial respiratory function and cell death in focal cerebral ischemia[J].J Neurochem,1999,73(3):1189-1199.
[14] CorbettD N.The problem of assessing effective neuroprotection in experimental cerebral ischemia[J].Prog Neurobiol,1998,54:531-548.
[15] DeVries A C,Nelson R J,Traystman R J,et al.Cognitive and behavioral assessment in experimental stroke research willitproveuseful [J].Neurosci Biobehav Rev,2001,25(4):325-342.
[2] Mutsuki K T F.Direct experimental middle cerebral artery induces high reproducibility of brain ischemia in mice[J].Exp Anim,2009,58(1):19-29.
[3] Longa E Z,Weinstein P R,Carlson S.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20(1):84-91.
[4] Mahmood A L D,Wang L.Treatment of traumatic brain injury in female rats with intravenous administration of bone marrow stromal cells[J].Neurosurgery,2001,49:1196-1204.
[5] Warlow C P.Epidemiology of stroke[J].Lancet,1998,352(Suppl3):SIII1-SIII4.
[6] Tomsick T A,Khatri P,Jovin T,et al.Equipoise among recanalization strategies[J].Neurology,2010,74(13):1069-1076.
[7] 杨慧,董小黎.脑缺血动物模型研究进展[J].实验动物科学,2007,24(1):38-40.
[8] Tamura A,Graham D I,Mcculloch J,et al.Focal cerebral ischaemia in the rat:1.Description of technique and early neuropathological consequences following middle cerebral artery occlusion[J].Journal of Cerebral Blood Flow & Metabolism Official Journal of the International Society of Cerebral Blood Flow & Metabolism,1981,1(1):53-60.
[9] Longa E Z,Weinstein P R,Carlson S,et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20(1):84-91.
[10] 杨渊,郭瑞友,张苏明,等.光化学诱导老年大鼠局灶性脑梗塞模型的研究[J].中国老年学杂志,2001,21(1):35-38.
[11] 郑冲.局灶性脑缺血动物模型制作方法的概述[J].中国卒中杂志,2008,3(8):603-607.
[12] 翟小虎,王斌,宋亚刚,等.线栓法制备大鼠脑缺血模型的研究进展与思路[J].实验动物科学,2015,32(5):55-58.
[13] Anderson M F,Sims N R.Mitochondrial respiratory function and cell death in focal cerebral ischemia[J].J Neurochem,1999,73(3):1189-1199.
[14] CorbettD N.The problem of assessing effective neuroprotection in experimental cerebral ischemia[J].Prog Neurobiol,1998,54:531-548.
[15] DeVries A C,Nelson R J,Traystman R J,et al.Cognitive and behavioral assessment in experimental stroke research willitproveuseful [J].Neurosci Biobehav Rev,2001,25(4):325-342.
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