放线菌KN37菌株代谢物对番茄灰霉病的防治
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摘要
采用离体和活体试验,研究KN37菌株代谢物对番茄灰霉病的防治效果,并利用分子手段对KN37菌株进行了初步鉴定。结果表明,放线菌KN37菌株代谢物可强烈抑制番茄灰霉病菌的菌丝生长和孢子萌发,对细菌性斑点病菌、番茄早疫病菌和油菜菌核病菌的抑制率达到90%以上,且对葡萄灰霉病具有较好的防治效果。由此可见,放线菌KN37菌株代谢物具有较好的开发潜力。
In order to clarify the control effect of the fermentation product of actinomycete strain KN37 on plant diseases, we determined the antimicrobial spectrum of its broth in vitro and in vivo, and identified KN37 strain at a molecular level. The results showed that KN37 strain had a high inhibitory effect on mycelial growth and spore germination of Botrytis cinerea. This strain also showed high inhibition activities on Pseudomonas syringae, Alternaria solani and Botrytis cinereal, with their inhibition rate being higher than 90% The strain KN37 had a high control effect on tomato gray mold in vivo, and, therefore, has a potential of research and development in the field of microbial pesticides.
In order to clarify the control effect of the fermentation product of actinomycete strain KN37 on plant diseases, we determined the antimicrobial spectrum of its broth in vitro and in vivo, and identified KN37 strain at a molecular level. The results showed that KN37 strain had a high inhibitory effect on mycelial growth and spore germination of Botrytis cinerea. This strain also showed high inhibition activities on Pseudomonas syringae, Alternaria solani and Botrytis cinereal, with their inhibition rate being higher than 90% The strain KN37 had a high control effect on tomato gray mold in vivo, and, therefore, has a potential of research and development in the field of microbial pesticides.
引文
[1] 李芳,陈建明,陈忠其,等.植物免疫诱抗剂对番茄灰霉病和早疫病的抑菌效果[J].农药,2016,55(3):214-216.
[2] 赵杨,苗则彦,李颖,等.番茄灰霉病防治研究进展[J].中国植保导刊,2014,34(7):21-29.
[3] LECHENET M,DESSAINT F,PY G,et al.Reducing pesticide use while preserving crop productivity and profitability on arable farms[J].Nature Plants,2017,3(3):17008.
[4] TILMAN D,CLARK M,WILLIAMS D R,et al.Future threats to biodiversity and pathways to their prevention[J].Nature,2017,546(7656):73-81.
[5] WENCEWICZ T A.New antibiotics from nature's chemical inventory[J].Bioorganic & Medicinal Chemistry,2016,24(24):6227-6252.
[6] 黄剑,李天华,崔艺久,等.放线菌H50发酵液抑菌活性及其稳定性测定[J].沈阳农业大学学报,2012,43(3):311-315.
[7] 张波,吴文君,宗兆锋.放线菌Z_(139)菌株的分离、鉴定及其生物活性[J].西北农林科技大学学报(自然科学版),2005,33(8):69-72.
[8] 张宁.内蒙荒漠盐碱土壤中放线菌和嗜盐细菌的选择性分离及杀虫抑菌活性筛选[D].杨凌:西北农林科技大学,2012.
[9] 邱珂.甲基营养型芽孢杆菌Lw-6防治灰霉病研究[D].杨凌:西北农林科技大学,2018.
[10] 阮继生,黄英.放线菌快速鉴定与系统分类[M].北京:科学出版社,2011.
[11] 王秀爽,田江丽,邵胜楠,等.新疆天池放线菌的分离及其抑菌活性[J].西北农业学报,2018,27(11):1660-1666.
[12] HAN L R,ZHANG G Q,MIAO G P,et al.Streptomyces kanasensis sp.nov.,an antiviral glycoprotein producing actinomycete isolated from forest soil around Kanas[J].Current Microbiology,2015,71:627–631.
[13] ZHANG G Q,FENG J T,HAN L R,et al.Antiviral activity of glycoprotein GP-1 isolated from Streptomyces kanasensis ZX01[J].International Journal of Biological Macromolecules,2016,88:572–577.
[14] 张国强.青藏高原土壤放线菌分离及其农用活性初步研究[D].杨凌:西北农林科技大学,2012.
[15] 张淑曼.富产卡那霉素B菌株的构建及其调控基因的研究[D].北京:北京化工大学,2018.
[16] SUBEDI Y P,ALFINDEE M N,TAKEMOTO J Y,et al.Antifungal amphiphilic kanamycins:new life for an old drug[J].Medchemcomm,2018,9(6):909-919.
[2] 赵杨,苗则彦,李颖,等.番茄灰霉病防治研究进展[J].中国植保导刊,2014,34(7):21-29.
[3] LECHENET M,DESSAINT F,PY G,et al.Reducing pesticide use while preserving crop productivity and profitability on arable farms[J].Nature Plants,2017,3(3):17008.
[4] TILMAN D,CLARK M,WILLIAMS D R,et al.Future threats to biodiversity and pathways to their prevention[J].Nature,2017,546(7656):73-81.
[5] WENCEWICZ T A.New antibiotics from nature's chemical inventory[J].Bioorganic & Medicinal Chemistry,2016,24(24):6227-6252.
[6] 黄剑,李天华,崔艺久,等.放线菌H50发酵液抑菌活性及其稳定性测定[J].沈阳农业大学学报,2012,43(3):311-315.
[7] 张波,吴文君,宗兆锋.放线菌Z_(139)菌株的分离、鉴定及其生物活性[J].西北农林科技大学学报(自然科学版),2005,33(8):69-72.
[8] 张宁.内蒙荒漠盐碱土壤中放线菌和嗜盐细菌的选择性分离及杀虫抑菌活性筛选[D].杨凌:西北农林科技大学,2012.
[9] 邱珂.甲基营养型芽孢杆菌Lw-6防治灰霉病研究[D].杨凌:西北农林科技大学,2018.
[10] 阮继生,黄英.放线菌快速鉴定与系统分类[M].北京:科学出版社,2011.
[11] 王秀爽,田江丽,邵胜楠,等.新疆天池放线菌的分离及其抑菌活性[J].西北农业学报,2018,27(11):1660-1666.
[12] HAN L R,ZHANG G Q,MIAO G P,et al.Streptomyces kanasensis sp.nov.,an antiviral glycoprotein producing actinomycete isolated from forest soil around Kanas[J].Current Microbiology,2015,71:627–631.
[13] ZHANG G Q,FENG J T,HAN L R,et al.Antiviral activity of glycoprotein GP-1 isolated from Streptomyces kanasensis ZX01[J].International Journal of Biological Macromolecules,2016,88:572–577.
[14] 张国强.青藏高原土壤放线菌分离及其农用活性初步研究[D].杨凌:西北农林科技大学,2012.
[15] 张淑曼.富产卡那霉素B菌株的构建及其调控基因的研究[D].北京:北京化工大学,2018.
[16] SUBEDI Y P,ALFINDEE M N,TAKEMOTO J Y,et al.Antifungal amphiphilic kanamycins:new life for an old drug[J].Medchemcomm,2018,9(6):909-919.