周丛生物对罗丹明B的吸附降解特性
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摘要
探讨在添加或不添加NaN_3条件下,不同周丛生物投放量(干质量0.1、0.2、0.3、0.4 g/L),温度(10、15、20、25、30、35℃),pH(4、6、8、10)和起始罗丹明B质量浓度(1、5、10、20、40、80、160 mg/L)对周丛生物对罗丹明B的吸附和降解特性的影响,并通过HPLC–MS分析周丛生物对罗丹明B的降解机理。结果表明:周丛生物对罗丹明B具有一定的吸附和降解能力,在本试验周丛生物的用量范围内,随着用量的增加,周丛生物对罗丹明B的去除率逐渐增加;其过程主要是2阶段的协同过程,涉及生物吸附和生物降解,以吸附为主;不添加NaN_3的周丛生物去除罗丹明B的最佳pH为6,温度为30℃;添加NaN_3的周丛生物对罗丹明B的最佳吸附pH为6,温度为20～30℃;等温线以Langmuir模型和Freundlich模型拟合,周丛生物+NaN_3组更符合Langmuir模型;HPLC–MS检测结果表明,周丛生物降解罗丹明B产生1个相对分子质量约为391的中间产物,其降解过程可能是一个逐步脱乙基的过程。
This study investigated the adsorption and degradation characteristics of rhodamine B(RhB) by periphyton with or without NaN_3, under a group of the dosage of periphyton(dry mass 0.1, 0.2, 0.3, 0.4 g/L), temperatures(10, 15, 20,25, 30, 35 ℃), pH values(4, 6, 8, 10) and the initial mass concentration of RhB(1, 5, 10, 20, 40, 80, 160 mg/L). This study also investigated the degradation mechanism of RhB by periphyton with HPLC-MS detection. The results showed that periphyton had some functions in biosorption and degradation of RhB. Within the dosage of periphyton in this experiment, the removal rate towards RhB increased gradually with the raise of periphyton. The removal procedure of RhB by periphyton included two-stage synergistic processes, involving biosorption and biodegradation, with biosorption as the dominant process. The optimal pH and temperature for removal of RhB by periphyton without NaN_3 were 6 and30 ℃, respectively; the optimal pH and temperature for adsorption of RhB by periphyton with NaN_3 were 6 and 20-30 ℃,respectively. The isotherm was fitted by the Langmuir model and the Freundlich model, meanwhile, the Langmuir model was more suitable in explaining the adsorption isotherm of RhB by periphyton with NaN_3. The results of HPLC-MS detection showed that the degradation of RhB might produced an intermediate product with a molecular weight of about391 and the degradation process involved a gradual deethylation.
This study investigated the adsorption and degradation characteristics of rhodamine B(RhB) by periphyton with or without NaN_3, under a group of the dosage of periphyton(dry mass 0.1, 0.2, 0.3, 0.4 g/L), temperatures(10, 15, 20,25, 30, 35 ℃), pH values(4, 6, 8, 10) and the initial mass concentration of RhB(1, 5, 10, 20, 40, 80, 160 mg/L). This study also investigated the degradation mechanism of RhB by periphyton with HPLC-MS detection. The results showed that periphyton had some functions in biosorption and degradation of RhB. Within the dosage of periphyton in this experiment, the removal rate towards RhB increased gradually with the raise of periphyton. The removal procedure of RhB by periphyton included two-stage synergistic processes, involving biosorption and biodegradation, with biosorption as the dominant process. The optimal pH and temperature for removal of RhB by periphyton without NaN_3 were 6 and30 ℃, respectively; the optimal pH and temperature for adsorption of RhB by periphyton with NaN_3 were 6 and 20-30 ℃,respectively. The isotherm was fitted by the Langmuir model and the Freundlich model, meanwhile, the Langmuir model was more suitable in explaining the adsorption isotherm of RhB by periphyton with NaN_3. The results of HPLC-MS detection showed that the degradation of RhB might produced an intermediate product with a molecular weight of about391 and the degradation process involved a gradual deethylation.
引文
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[16]张卉灵,邵继海,匡晓琳,等.水华蓝藻胞外聚合物对高岭土吸附罗丹明B行为的影响[J].净水技术,2017,36(10):46-51,63.
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[19]陆晶,黄丽,邵春雷,等.高铁酸钾氧化降解罗丹明(Rh B)水溶液的研究[J].环境化学,2007,26(3):366-370.
[20]杨继亮,岳贤田.膨润土-壳聚糖复合材料对罗丹明吸附行为的研究[J].环境保护科学,2017,53(3):91-94.
[21]王章鸿,郭海艳,沈飞,等.蚯蚓粪便制备生物炭及其对罗丹明B吸附的研究[J].环境科学学报,2015,35(10):3170-3177.
[22]蒲丽君.小麦秸秆吸附剂的制备及其对溶液中铜离子的吸附性能研究[D].兰州:兰州理工大学,2011.
[23]杨丽华,龚道新,周健,等.二氯喹啉酸在几种矿物上的吸附特性及其机理[J].湖南农业大学学报(自然科学版),2015,41(2):202-208.
[24]刘华俊,彭天右,彭正合,等.Dy/WO3光催化降解罗丹明B的反应机理[J].武汉大学学报(理学版),2007,53(2):127-132.
[2]曾艳.可见光光催化降解染料废水的研究[D].南昌:南昌大学,2010.
[3]李晓.铁循环功能菌群解钝化及强化海绵铁染料脱色的作用[D].广州:广州大学,2015.
[4]陈梅兰.罗丹明B的危害及其在食品中快速检测方法的建立[D].福州:福州大学,2014.
[5]高锦章,曾丽娟,任杰.用碘离子修饰的B-Z化学振荡反应测定辣椒面中的罗丹明B[J].湖南农业大学学报(自然科学版),2013,39(3):327-330.
[6]WANG Z P,XUE M M,HUANG K,et al.Textile dyeing wastewater treatment[M]//HAUSER P.Advances in Treating Textile Effluent.Rijeka:InTech,2011:91-116.
[7]PARK C,LEE M,LEE B,et al.Biodegradation and biosorption for decolorization of synthetic dyes by Funalia trogii[J].Biochemical Engineering Journal,2007,36(1):59-65.
[8]JADHAV U U,DAWKAR V V,JADHAV M U,et al.Decolorization of the textile dyes using purified banana pulp polyphenol oxidase[J].International Journal of Phytoremediation,2011,13(4):357-372.
[9]GHODAKE G,JADHAV U,TAMBOLI D,et al.Decolorization of textile dyes and degradation of mono-azo dye amaranth by Acinetobacter calcoaceticus NCIM 2890[J].Indian Journal of Microbiology,2011,51(4):501-508.
[10]王逢武,刘玮,万娟娟,等.周丛生物存在下不同水层氧化还原带的分布及其与微生物的关联[J].环境科学,2015,36(11):4043-4050.
[11]WU Y H,LIU J T,YANG L Z,et al.Allelopathic control of cyanobacterial blooms by periphyton biofilms[J].Environmental Microbiology,2011,13(3):604-615.
[12]DU B W,HADDAD S P,SCOTT W C,et al.Pharmaceutical bioaccumulation by periphyton and snails in an effluent-dependent stream during an extreme drought[J].Chemosphere,2015,119:927-934.
[13]陈重军,韩志英,朱荫湄等.周丛藻类及其在水质净化中的应用[J].应用生态学报,2009,20(11):2820-2826.
[14]SHABBIR S,FAHEEM M,ALI N,et al.Periphyton biofilms:a novel and natural biological system for the effective removal of sulphonated azo dye methyl orange by synergistic mechanism[J].Chemosphere,2017,167:236-246.
[15]SAISHO D,NAKAZONO M,TSUTSUMI N,et al.ATPsynthesis inhibitors as well as respiratory inhibitors increase steady-state level of alternative oxidase m RNAin Arabidopsis thaliana[J].Journal of Plant Physiology,2001,158(2):241-245.
[16]张卉灵,邵继海,匡晓琳,等.水华蓝藻胞外聚合物对高岭土吸附罗丹明B行为的影响[J].净水技术,2017,36(10):46-51,63.
[17]THAO T P,KAO H C,JUANG R S,et al.Kinetic characteristics of biodegradation of methyl orange by Pseudomonas putida,mt2 in suspended and immobilized cell systems[J].Journal of the Taiwan Institute of Chemical Engineers,2013,44(5):780-785.
[18]MNIF I,MAKTOUF S,FENDRI R,et al.Improvement of methyl orange dye biotreatment by a novel isolated strain,Aeromonas veronii GRI,by SPB1 biosurfactant addition[J].Environmental Science and Pollution Research,2016,23(2):1742-1754.
[19]陆晶,黄丽,邵春雷,等.高铁酸钾氧化降解罗丹明(Rh B)水溶液的研究[J].环境化学,2007,26(3):366-370.
[20]杨继亮,岳贤田.膨润土-壳聚糖复合材料对罗丹明吸附行为的研究[J].环境保护科学,2017,53(3):91-94.
[21]王章鸿,郭海艳,沈飞,等.蚯蚓粪便制备生物炭及其对罗丹明B吸附的研究[J].环境科学学报,2015,35(10):3170-3177.
[22]蒲丽君.小麦秸秆吸附剂的制备及其对溶液中铜离子的吸附性能研究[D].兰州:兰州理工大学,2011.
[23]杨丽华,龚道新,周健,等.二氯喹啉酸在几种矿物上的吸附特性及其机理[J].湖南农业大学学报(自然科学版),2015,41(2):202-208.
[24]刘华俊,彭天右,彭正合,等.Dy/WO3光催化降解罗丹明B的反应机理[J].武汉大学学报(理学版),2007,53(2):127-132.
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