PCPs污染物胁迫对禾花鲤不同组织抗氧化系统的影响
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摘要
探讨了不同浓度PCPs污染物(0,4,8,16 mg/L)对禾花鲤不同组织抗氧化系统的影响。PCPs污染物胁迫24 h后,对禾花鲤肝胰腺、肌肉、鳃、脾脏、脑、肾脏、心脏等组织抗氧化系统(T-AOC、SOD、CAT、MDA、GST、GSH)的活性分析表明,禾花鲤不同器官组织抗氧化活性不同,T-AOC、CAT和GSH活性具有组织分布特异性。鳃组织中GST、GSH、MDA,肝胰脏中的GST,肾脏中T-AOC、GST和脑组织的T-AOC能有效反映组织的氧化损伤,可作为PCPs污染物对禾花鲤毒性效应的敏感指标。
In this experiment, the effects of different concentrations of PCPs pollutants(0, 4, 8, 16 mg/L) on the antioxidative systems in different tissues of Procypris mera were studied. The activities of the total antioxidant capacity(T-AOC),superoxide dismutase(SOD), catalase(CAT), maleic dialdehyde(MDA), glutathione S-transferase(GST) and glutathione(GSH) in different tissues(hepatopancreas, muscle, gill, spleen, brain and kidney) of the fish were analyzed after 24 h exposure to PCPs. The results showed that the antioxidant activities in the tested organs were significantly different, and T-AOC, CAT and GSH activity had tissue-specific distribution. In addition, GST, GSH and MDA in the gill, GST in the hepatopancreas, T-AOC and GST in the kidney, and T-AOC in the brain can effectively respond to the oxidative damage of tissues, which are sensitive indicators of PCPs toxicity to Procypris mera.
In this experiment, the effects of different concentrations of PCPs pollutants(0, 4, 8, 16 mg/L) on the antioxidative systems in different tissues of Procypris mera were studied. The activities of the total antioxidant capacity(T-AOC),superoxide dismutase(SOD), catalase(CAT), maleic dialdehyde(MDA), glutathione S-transferase(GST) and glutathione(GSH) in different tissues(hepatopancreas, muscle, gill, spleen, brain and kidney) of the fish were analyzed after 24 h exposure to PCPs. The results showed that the antioxidant activities in the tested organs were significantly different, and T-AOC, CAT and GSH activity had tissue-specific distribution. In addition, GST, GSH and MDA in the gill, GST in the hepatopancreas, T-AOC and GST in the kidney, and T-AOC in the brain can effectively respond to the oxidative damage of tissues, which are sensitive indicators of PCPs toxicity to Procypris mera.
引文
[1]黄珂,赵东豪,杨宏亮,等.渔业环境及水产品中药物和个人护理用品(PPCPs)的研究进展[J].南方水产科学,2016,12(3):119-126.
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[13]麻艳群,卢克焕,黄凯,等.Cu2+胁迫对禾花鲤(Procypris mterus)过氧化氢酶活性的影响[J].安徽农业大学学报,2010,37(4):623-626.
[14]谭树华,何典翼,严芳,等.亚硝酸钠对鲫鱼肝脏丙二醛含量和总抗氧化能力的影响[J].农业环境科学学报,2005,24(S):21-24.
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[16]熊铧龙,蒋左玉,张儒学,等.盐酸恩诺沙星对杂交鲟幼鱼肝脏抗氧化酶活性的影响[J].水产科学,2017,36(5):601-605.
[17]王桂燕,李锋,史济月,等.四氯乙烯胁迫对草鱼抗氧化酶活性的影响及其机理[J].中国环境科学,2014,34(6):1579-1585.
[18]王丽,张耘泽,耿歌,等.高效氯氰菊酯急性暴露中斑马鱼相关酶活性和基因表达变化[J].生态毒理学报,2016,11(4):146-154.
[19]姚玲霞,宋猛猛,周迎,等.锌、镉复合暴露对铜锈环棱螺内脏抗氧化酶活性和丙二醛含量的影响[J].环境化学,2015,34(12):2316-2318.
[20]任洪涛,张春暖,林霖.Mo6+对草鱼组织器官及抗氧化酶活性的影响[J].水产科学,2017,36(3):317-322.
[21]张鼎元,曹潇,郭春阳,等.乙酸铜对银鲳幼鱼急性毒性及抗氧化酶活性的影响[J].生态毒理学报,2016,11(4):280-286.
[22]Stebbing A R D.Hormesis—The stimulation of growth by low levels of inhibitors [J].Science of the Total Environment,1982,22(3):213-234.
[23]尹晓燕,田兴,李大鹏,等.亚硝态氮对草鱼离体肝细胞抗氧化体系的影响[J].淡水渔业,2014,44(5):49-53.
[24]叶俊,肖琛,尹晓燕,等.亚硝态氮胁迫对草鱼非特异性免疫性能的影响[J].淡水渔业,2013,43(4):40-44.
[2]Klosterhaus S L,Grace R,Hamilton M C,et al.Method validation and reconnaissance of pharmaceuticals,personal care products,and alkylphenols in surface waters,sediments,and mussels in an urban estuary [J].Environment International,2013,54:92-99.
[3]胡君剑,胡霞林,尹大强.药品与个人护理品在鱼体中的累积及代谢研究进展[J].生态毒理学报,2015,10(2):89-99.
[4]Yao L,Zhao J L,Liu Y S,et al.Simultaneous determination of 24 personal care products in fish muscle and liver tissues using QuEChERS extraction coupled with ultra pressure liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometer analyses [J].Analytical and Bioanalytical Chemistry,2016,408:8177-8193.
[5]刘娜,金小伟,王业耀,等.我国地表水中药物与个人护理品污染现状及其繁殖毒性筛查[J].生态毒理学报,2015,10(6):1-12.
[6]贾瑷,胡建英,孙建仙,等.环境中的医药品与个人护理品[J].化学进展,2009,21(2/3):389-399.
[7]Nassef M,Matsumoto S,Seki M,et al.Acute effects of triclosan,diclofenac and carbamazepine on feeding performance of Japanese medaka fish(Oryzias latipes)[J].Chemosphere,2010,80(9):1095-1100.
[8]Cunha V,Burkhardt-Medicke K,Wellner P,et al.Effects of pharmaceuticals and personal care products (PPCPs) on multixenobiotic resistance (MXR) related efflux transporter activity in zebrafish (Danio rerio) embryos [J].Ecotoxicology and Environmental Safety,2017,136:14-23.
[9]孙洪芹,江文静,郭艳敏,等.个人护理用品(PCPs)的生态毒性和处理工艺效果研究进展[J].生态毒理学报,2016,11(1):94-102.
[10]Winston G W.Oxidants and antioxidants in aquatic animals[J].Comparative Biochemistry and Physiology Part C:Comparative Pharmacology,1991,100(1-2):173-176.
[11]Valavanidis A,Vlahogianni T,Dassenakis M,et al.Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants [J].Ecotoxicology and Environmental Safety,2006,64:178-189.
[12]孙翰昌,徐敬明,甘桂云.4种重金属对禾花鱼胚胎的毒性效应[J].水生态学杂志,2013,34(1):92-95.
[13]麻艳群,卢克焕,黄凯,等.Cu2+胁迫对禾花鲤(Procypris mterus)过氧化氢酶活性的影响[J].安徽农业大学学报,2010,37(4):623-626.
[14]谭树华,何典翼,严芳,等.亚硝酸钠对鲫鱼肝脏丙二醛含量和总抗氧化能力的影响[J].农业环境科学学报,2005,24(S):21-24.
[15]张春玲,胡俊峰,王丕文,等.苯并(a)芘对鲫鱼肝脏总抗氧化能力的影响[J].环境与健康杂志,2004,21(5):325-326.
[16]熊铧龙,蒋左玉,张儒学,等.盐酸恩诺沙星对杂交鲟幼鱼肝脏抗氧化酶活性的影响[J].水产科学,2017,36(5):601-605.
[17]王桂燕,李锋,史济月,等.四氯乙烯胁迫对草鱼抗氧化酶活性的影响及其机理[J].中国环境科学,2014,34(6):1579-1585.
[18]王丽,张耘泽,耿歌,等.高效氯氰菊酯急性暴露中斑马鱼相关酶活性和基因表达变化[J].生态毒理学报,2016,11(4):146-154.
[19]姚玲霞,宋猛猛,周迎,等.锌、镉复合暴露对铜锈环棱螺内脏抗氧化酶活性和丙二醛含量的影响[J].环境化学,2015,34(12):2316-2318.
[20]任洪涛,张春暖,林霖.Mo6+对草鱼组织器官及抗氧化酶活性的影响[J].水产科学,2017,36(3):317-322.
[21]张鼎元,曹潇,郭春阳,等.乙酸铜对银鲳幼鱼急性毒性及抗氧化酶活性的影响[J].生态毒理学报,2016,11(4):280-286.
[22]Stebbing A R D.Hormesis—The stimulation of growth by low levels of inhibitors [J].Science of the Total Environment,1982,22(3):213-234.
[23]尹晓燕,田兴,李大鹏,等.亚硝态氮对草鱼离体肝细胞抗氧化体系的影响[J].淡水渔业,2014,44(5):49-53.
[24]叶俊,肖琛,尹晓燕,等.亚硝态氮胁迫对草鱼非特异性免疫性能的影响[J].淡水渔业,2013,43(4):40-44.
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