基于高通量测序的大叶女贞(Ligustrum lucidum)转录组分析
|
摘要
为研究大叶女贞功能基因的序列信息和表达状况,以幼嫩叶片为材料,利用Illumina HiSeqTM2500高通量测序技术对其进行转录组测序,通过序列拼接组装得到192 392个单基因簇(Unigene),平均长度为821 bp。NR库和Swiss-Prot库分别注释了88 896和61 783条Unigene。在GO分子功能、生物过程和细胞组分3大类中分别注释了12 914、12 859和7 644个基因,其中以分子功能注释最多,占38.64%;以细胞组分较少,为22.87%。本研究获得的转录组数据将为大叶女贞功能基因挖掘与利用和其种质资源遗传改良等方面的研究提供参考。
In order to study the sequence information and expression status of the functional gene of Euphorbia Ligustrum lucidum. In this stud y, young leaves were used as materials and transcriptome sequencing was performed by Illumina HiSeqTM2500 high-throughput sequencing technology. Sequence splicing assembly yielded192 392 single gene clusters(Unigene) with an average length of 821 bp. The NR library and the SWISS-Prot library commented 88 896 and 61 783 Unigenes, respectively. 12 914, 12 958 and 7 644 Unigenes were annotated in gene ontology(GO) and grouped into biological processes, cellular components and molecular function three functional categories. The molecular function were most while the cellular components were least, account for 38.64%and 22.87%. These transcriptome data of Ligustrum lucidum would be helpful for the gene discovery and utilization, molecular marker assisted breeding and genetic improvement of Ligustrum lucidum in the further research.
In order to study the sequence information and expression status of the functional gene of Euphorbia Ligustrum lucidum. In this stud y, young leaves were used as materials and transcriptome sequencing was performed by Illumina HiSeqTM2500 high-throughput sequencing technology. Sequence splicing assembly yielded192 392 single gene clusters(Unigene) with an average length of 821 bp. The NR library and the SWISS-Prot library commented 88 896 and 61 783 Unigenes, respectively. 12 914, 12 958 and 7 644 Unigenes were annotated in gene ontology(GO) and grouped into biological processes, cellular components and molecular function three functional categories. The molecular function were most while the cellular components were least, account for 38.64%and 22.87%. These transcriptome data of Ligustrum lucidum would be helpful for the gene discovery and utilization, molecular marker assisted breeding and genetic improvement of Ligustrum lucidum in the further research.
引文
Ao Y.,Ma L.Y.,Han S.W.,and Yang X.H.,2015,Transcriptome analysis for Xanthoceras so rbifolia bunge based on highthroughput sequencing technology,Zhongguo Shengwu Gongcheng Zazhi(China Biotechnology),35(7):22-29(敖妍,马履一,韩树文,杨晓辉,2015,基于高通量测序的文冠果转录组分析,中国生物工程杂志,35(7):22-29)
Cao Y.C.,Liu R.N.,and Zhao Z.L.,2014,Effects of drought Stress on physiological index of Ligstrun lucidum ait,He'nan Nongye Kexue(Journal of He'nan Agricultural Science),43(8):102-105(曹艳春,刘荣宁,赵振利,2014,干旱胁迫对女贞生理指标的影响,河南农业科学,43(8):102-105)
Chen L.X.,2017,Molecular basis of Antonasparo locustae parasite in locust,Dissertation for Ph.D.,China Agriculture University,Supervisor:Zhang L.,pp.59-62(陈龙欣,2017,蝗虫微孢子虫寄生飞蝗的分子基础,博士学位论文,中国农业大学,导师:张龙,pp.59-62)
Ge M.K.,Wang D.L.,Zhou Y.C.,and He T.,2015,Effects of nitrogen on some physiological characteristics and inclusion contents in leaves of Ligustrum robustum,Linye Keji Kaifa(China Forestry Science and Technology),(29):37-40(葛敏凯,王德炉,周云超,何滔,2015,氮对粗壮女贞叶片生理特性及内含物含量的影响,林业科技开发,(29):37-40)
Hao M.Z.,Han M.H.,Peng F.R.,Liang Y.W.,Zhao M.M.,and Xu S.,2014,A comperative contrast study on the cold-resistance of four Ligustrum cultivars,Jiangxi Nongye Daxue Xuebao(Acta Agriculturae Universitatis Jiangxiensis),33(6):1094-1099(郝明灼,韩明慧,彭方仁,梁有旺,赵明明,徐松,2011,4个女贞品种抗寒性比较,江西农业大学学报,33(6):1094-1099)
He W.,Wu Y.X.,Feng J.C.,Li J.D.,Wang N.,Bi H.T.,Guo J.S.,and Si C.Z.,2005,Influence of exogenous ABA on photosynthesis and transpiration rate of Ligustrum lucidum Ait,He'nan Nongye Daxue Xuebao(Journal of He'nan Agricultural University),39(2):167-170(何威,武应霞,冯建灿,李继东,王念,毕会涛,郭建设,司长征,2005,外源ABA对女贞光合及蒸腾速率的影响,河南农业大学学报,39(2):167-170)
Huang H.,Liang Z.S.,and Han R.L.,2008,Effects of drought stress on morphology and growth of Ligustrum lucidum,Linye Kexue(Scientia Silvae Sinica),44(8):145-148(黄华,梁宗锁,韩蕊莲,2008,持续干旱胁迫对女贞形态与生长的影响,林业科学,44(8):145-148)
Liu H.,Li D.J.,and Deng Z.,2014,Advances in research of transcriptional regulatory network in response to cold stress in plants,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(18):3523-3533(刘辉,李德军,邓治,2014,植物应答低温胁迫的转录调控网络研究进展,中国农业科学,47(18):3523-3533)
Wang Y.S.,Nie F.,and Lin S.Q.,2015,The latest research of progress in high-throughput sequencing of fruit tree,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(9):2034-2043(王云生,聂飞,林顺权,2015,果树高通量测序的最新研究进展,基因组学与应用生物学,34(9):2034-2043)
Wang Z.X.,2017,Actinidia kolomikta(Rupr.&Maxim.)maxim variegated leaf formation mechanism and photosynthetic function,Dissertation for Ph.D.,Chinese Academy of Agriculture Sciences,Supervisor:Ai J.,pp.65-69(王振兴,2017,狗枣猕猴桃彩叶形成及其光合功能研究,博士学位论文,中国农业科学院,导师:艾军,pp.65-69)
Zhan K.,2017,SCFAs regulate the inflammatory response of bovine rumem epithelial cells by GPR41,Dissertation for Ph.D.,Yanzhou University,Supervisor:Zhao G.Q.,pp.53-60(詹康,2017,SCFAs通过GPR41调控奶牛瘤胃上皮细胞炎症反应的研究,博士学位论文,扬州大学,导师:赵国琦,pp.53-60)
Zhao S.Q.,and Dong J.J.,2015,Genetic regulation of anther and pollen development in Arabidopsis thaliana,Shanxi Daxue Xuebao(Journal of Shanxi University(Natural Science Edition)),38(1):177-184(赵淑清,董晶晶,2015,拟南芥花药和花粉发育的分子调控机制,山西大学学报(自然科学版),38(1):177-184)
Cao Y.C.,Liu R.N.,and Zhao Z.L.,2014,Effects of drought Stress on physiological index of Ligstrun lucidum ait,He'nan Nongye Kexue(Journal of He'nan Agricultural Science),43(8):102-105(曹艳春,刘荣宁,赵振利,2014,干旱胁迫对女贞生理指标的影响,河南农业科学,43(8):102-105)
Chen L.X.,2017,Molecular basis of Antonasparo locustae parasite in locust,Dissertation for Ph.D.,China Agriculture University,Supervisor:Zhang L.,pp.59-62(陈龙欣,2017,蝗虫微孢子虫寄生飞蝗的分子基础,博士学位论文,中国农业大学,导师:张龙,pp.59-62)
Ge M.K.,Wang D.L.,Zhou Y.C.,and He T.,2015,Effects of nitrogen on some physiological characteristics and inclusion contents in leaves of Ligustrum robustum,Linye Keji Kaifa(China Forestry Science and Technology),(29):37-40(葛敏凯,王德炉,周云超,何滔,2015,氮对粗壮女贞叶片生理特性及内含物含量的影响,林业科技开发,(29):37-40)
Hao M.Z.,Han M.H.,Peng F.R.,Liang Y.W.,Zhao M.M.,and Xu S.,2014,A comperative contrast study on the cold-resistance of four Ligustrum cultivars,Jiangxi Nongye Daxue Xuebao(Acta Agriculturae Universitatis Jiangxiensis),33(6):1094-1099(郝明灼,韩明慧,彭方仁,梁有旺,赵明明,徐松,2011,4个女贞品种抗寒性比较,江西农业大学学报,33(6):1094-1099)
He W.,Wu Y.X.,Feng J.C.,Li J.D.,Wang N.,Bi H.T.,Guo J.S.,and Si C.Z.,2005,Influence of exogenous ABA on photosynthesis and transpiration rate of Ligustrum lucidum Ait,He'nan Nongye Daxue Xuebao(Journal of He'nan Agricultural University),39(2):167-170(何威,武应霞,冯建灿,李继东,王念,毕会涛,郭建设,司长征,2005,外源ABA对女贞光合及蒸腾速率的影响,河南农业大学学报,39(2):167-170)
Huang H.,Liang Z.S.,and Han R.L.,2008,Effects of drought stress on morphology and growth of Ligustrum lucidum,Linye Kexue(Scientia Silvae Sinica),44(8):145-148(黄华,梁宗锁,韩蕊莲,2008,持续干旱胁迫对女贞形态与生长的影响,林业科学,44(8):145-148)
Liu H.,Li D.J.,and Deng Z.,2014,Advances in research of transcriptional regulatory network in response to cold stress in plants,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(18):3523-3533(刘辉,李德军,邓治,2014,植物应答低温胁迫的转录调控网络研究进展,中国农业科学,47(18):3523-3533)
Wang Y.S.,Nie F.,and Lin S.Q.,2015,The latest research of progress in high-throughput sequencing of fruit tree,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(9):2034-2043(王云生,聂飞,林顺权,2015,果树高通量测序的最新研究进展,基因组学与应用生物学,34(9):2034-2043)
Wang Z.X.,2017,Actinidia kolomikta(Rupr.&Maxim.)maxim variegated leaf formation mechanism and photosynthetic function,Dissertation for Ph.D.,Chinese Academy of Agriculture Sciences,Supervisor:Ai J.,pp.65-69(王振兴,2017,狗枣猕猴桃彩叶形成及其光合功能研究,博士学位论文,中国农业科学院,导师:艾军,pp.65-69)
Zhan K.,2017,SCFAs regulate the inflammatory response of bovine rumem epithelial cells by GPR41,Dissertation for Ph.D.,Yanzhou University,Supervisor:Zhao G.Q.,pp.53-60(詹康,2017,SCFAs通过GPR41调控奶牛瘤胃上皮细胞炎症反应的研究,博士学位论文,扬州大学,导师:赵国琦,pp.53-60)
Zhao S.Q.,and Dong J.J.,2015,Genetic regulation of anther and pollen development in Arabidopsis thaliana,Shanxi Daxue Xuebao(Journal of Shanxi University(Natural Science Edition)),38(1):177-184(赵淑清,董晶晶,2015,拟南芥花药和花粉发育的分子调控机制,山西大学学报(自然科学版),38(1):177-184)
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |