添加不同外源氮对长期秸秆还田土壤中氮素转化的影响
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摘要
【目的】秸秆还田能够改变土壤中各活性氮库的含量与比例,进而影响土壤氮素供应能力。本文研究了长期秸秆还田条件下添加不同外源氮对土壤中不同形态氮素的影响,旨在明确长期秸秆还田土壤活性氮库的含量差异。【方法】长期定位施肥试验点位于湖南省望城县(112°80′N、28°37′E,海拔高度100 m)。试验开始于1981年,供试土壤为第四纪红色黏土发育的水稻土,轮作制度为稻—稻—冬闲。2014年晚稻收获后,采集单施化肥和长期秸秆还田配施化肥两个处理的耕层土壤样品,开展室内培养试验。每个土壤样品设置灭菌和不灭菌两组主处理,在主处理下设:对照(CK)、添加尿素(N 150 kg/hm2,U)、添加秸秆(N 150 kg/hm2,S)和添加尿素和秸秆(N 300 kg/hm2,U+S)四个副处理,4次重复。在25℃下恒温培养5、10、20、30、50、90、130天时,分析土壤铵态氮、硝态氮、微生物氮和可溶性有机氮含量。【结果】1) U、S和U+S处理均显著提高土壤铵态氮和硝态氮含量,高低顺序为U> U+S> S> CK。非灭菌条件下,U处理的土壤铵态氮含量较其他处理高出90.8%～288%。2)灭菌后土壤铵态氮长期维持在较高水平,其向硝态氮转化过程受阻。在培养90天内,土壤硝态氮、微生物氮和可溶性有机氮含量均处于较低水平。3)而不灭菌条件下,各处理土壤硝态氮均在培养50天后迅速增加,至培养结束土壤硝态氮达最大值(117.43～243.17 mg/kg)。4)土壤微生物氮和可溶性有机氮分别于培养20天(106.72～244.01 mg/kg)和30天(95.76～140.63 mg/kg)时达到最大值。5)至培养结束,灭菌条件下长期NPKS土壤中U+S处理可溶性有机氮显著高于其他处理,较U和S处理分别提高51.55%和29.96%。【结论】添加不同外源氮有利于提高长期秸秆还田土壤中活性有机氮的含量,尤其是添加秸秆和尿素处理,能够显著提高土壤氮素的供应能力。
【Objectives】This paper was to study the effects of adding different N resources on various N forms in soils long-term applied by NPK with or without straw incorporated, aiming at analyzing the difference in the content of active N pools affected by long-term straw returning.【Methods】Surface soil samples from long-term chemical fertilization(NPK) and long-term chemical fertilization with straw incorporation(NPKS) treatments were collected after late rice harvest in 2014 in Wangcheng, Hunan. Indoor incubation experiment was carried out with two main treatments: soil with sterilization and non sterilization onto above-mentioned two kinds of soil samples, respectively. Each main treatment had four subsidiary treatments: the control(CK), application of urea(N 150 kg/hm2, U), application of straw(N 150 kg/hm2, S) and application of urea and straw(N 300 kg/hm2, U +S). Each treatment had four replications. Soil ammonium N(NH4+), nitrate N(NO3–), microbial N(MBN) and dissolved organic N(DON) were analyzed at various incubation dates.【Results】 1) The contents of soil NH4+and NO3– were significantly increased by urea, straw and urea with straw, with a sequence as U > U + S > S > CK.Under non-sterilization condition, soil NH4+ of U treatment was 90.85%–288.07% more than those of the other three treatments. 2) Under sterilization condition, soil NH4+ maintained at a high level, which would hinder the transformation process from NH4+ to NO3–. Soil contents of NO3–, MBN and DON were quite low in the first 90 days of incubation. 3) Under the condition of non-sterilization, soil NO3– increased rapidly after 50 days of incubation, reaching to the maximal value(117.43–243.17 mg/kg) at the end. 4) Soil MBN and DON reached the maximum value at 20 days(106.72–244.01 mg/kg) and 30 days(95.76–140.63 mg/kg), respectively. 5) At the end of incubation, under sterilization condition, soil DON content of U + S treatment from the long-term NPKS soil was significantly higher than those of the other treatments, with 51.55% and 29.96% more than the U and S treatments, respectively.【Conclusions】Adding different exogenous N sources is beneficial to increasing the content of active organic N pool in the soil with long-term straw returning especially adding straw and urea, which would improve soil N supply capacity significantly.
【Objectives】This paper was to study the effects of adding different N resources on various N forms in soils long-term applied by NPK with or without straw incorporated, aiming at analyzing the difference in the content of active N pools affected by long-term straw returning.【Methods】Surface soil samples from long-term chemical fertilization(NPK) and long-term chemical fertilization with straw incorporation(NPKS) treatments were collected after late rice harvest in 2014 in Wangcheng, Hunan. Indoor incubation experiment was carried out with two main treatments: soil with sterilization and non sterilization onto above-mentioned two kinds of soil samples, respectively. Each main treatment had four subsidiary treatments: the control(CK), application of urea(N 150 kg/hm2, U), application of straw(N 150 kg/hm2, S) and application of urea and straw(N 300 kg/hm2, U +S). Each treatment had four replications. Soil ammonium N(NH4+), nitrate N(NO3–), microbial N(MBN) and dissolved organic N(DON) were analyzed at various incubation dates.【Results】 1) The contents of soil NH4+and NO3– were significantly increased by urea, straw and urea with straw, with a sequence as U > U + S > S > CK.Under non-sterilization condition, soil NH4+ of U treatment was 90.85%–288.07% more than those of the other three treatments. 2) Under sterilization condition, soil NH4+ maintained at a high level, which would hinder the transformation process from NH4+ to NO3–. Soil contents of NO3–, MBN and DON were quite low in the first 90 days of incubation. 3) Under the condition of non-sterilization, soil NO3– increased rapidly after 50 days of incubation, reaching to the maximal value(117.43–243.17 mg/kg) at the end. 4) Soil MBN and DON reached the maximum value at 20 days(106.72–244.01 mg/kg) and 30 days(95.76–140.63 mg/kg), respectively. 5) At the end of incubation, under sterilization condition, soil DON content of U + S treatment from the long-term NPKS soil was significantly higher than those of the other treatments, with 51.55% and 29.96% more than the U and S treatments, respectively.【Conclusions】Adding different exogenous N sources is beneficial to increasing the content of active organic N pool in the soil with long-term straw returning especially adding straw and urea, which would improve soil N supply capacity significantly.
引文
[1]杨玉爱,王珂,叶正钱,魏幼璋.有机肥料资源及其对微量元素螯溶和利用研究[J].土壤通报,1994,25(7):21-25.Yang Y A,Wang K,Ye Z Q,Wei Y Z.Organic fertilizer resources and its utilization of dissolved trace elements[J].Chinese Journal of Soil Science,1994,25(7):21-25.
[2]赵士诚,曹彩云,李科江,等.长期秸秆还田对华北潮土肥力、氮库组分及作物产量的影响[J].植物营养与肥料学报,2014,20(6):1441-1449.Zhao S C,Cao C Y,Li K J,et al.Effects of long-term S return on soil fertility,nitrogen pool fractions and crop yields on a fluvo-aquic soil in North China[J].Journal of Plant Nutrition and Fertilizer,2014,20(6):1441-1449.
[3]赵俊晔,于振文,李延奇,王雪.施氮量对土壤无机氮分布和微生物量氮含量及小麦产量的影响[J].植物营养与肥料学报,2006,12(4):466-472.Zhao J Y,Yu Z W,Li Y Q,Wang X.Effects of nitrogen application rate on soil inorganic nitrogen distribution,microbial biomass nitrogen content and yield of wheat[J].Plant Nutrition and Fertilizer Science,2006,12(4):466-472.
[4]Yan D Z,Wang D J,Yang L Z.Long-term effect of chemical fertilizer,S,and manure on labile organic matter fractions in a paddy soil[J].Biology and Fertility of Soils,2007,44:93-101.
[5]Gardenas A I,Agren G I,Bird J A,et al.Knowledge gaps in soil carbon and nitrogen interactions-From molecular to global scale[J].Soil Biology&Biochemistry,2011,43(4):702-717.
[6]Soper F M,Paungfoo-Lonhienne C,Brackin R,Rentsch D,et al.Arabidopsis and Lobelia anceps access small peptides as a nitrogen source for growth[J].Functional Plant Biology,2011,38(10):788-796.
[7]Van Kessel C,Clough T,van Groenigen J W.Dissolved organic nitrogen:An overlooked pathway of nitrogen loss from agricultural systems[J].Journal of Environmental Quality,2009,38(2):393-401.
[8]Park J H,Kalbitz K,Matzner E.Resource control on the production of dissolved organic carbon and nitrogen in a deciduous forest floor[J].Soil Biology&Biochemistry,2002,34:813-822.
[9]Hedin L O,Armesto J J,Johnson A H.Patterns of nutrient loss from unpolluted,old-growth temperate forests:Evaluation of biogeochemical theory[J].Ecology,1995,76(2):493-509.
[10]Quaills R G,Haines B L,Swank W T.Fluxes of dissolved organic nutrients and humic substances in a deciduous forest[J].Ecology,1991,72(1):254-266.
[11]李贵桐,张宝贵,李保国.秸秆预处理对土壤微生物量及呼吸活性的影响[J].应用生态学报,2003,14(12):2225-2228.Li G T,Zhang B G,Li B G.Effect of S pretreatment on soil microbial biomass and respiration activity[J].Chinese Journal of Applied Ecology,2003,14(12):2225-2228.
[12]练成燕,张桃林,王兴祥.有机物料对红壤几种形态碳氮及酸度的影响[J].中国农业科学,2009,42(11):3922-3932.Lian C Y,Zhang T L,Wang X X.Effects of organic materials on several forms of soil carbon and nitrogen and soil acidity[J].Scientia Agricultura Sinica,2009,42(11):3922-3932.
[13]Recous S,Robin D,Darwis D,et al.Soil inorganic N availability:Effect on maize residue decomposition[J].Soil Biology&Biochemistry,1995,27(12):1529-1538.
[14]朱兆良,金继运.保障我国粮食安全的肥料问题[J].植物营养与肥料学报,2013,19(2):259-273.Zhu Z L,Jin J Y.Fertilizer use and food security in China[J].Plant Nutrition and Fertilizer Science,2013,19(2):259-273.
[15]王如芳,张吉旺,董树亭,刘鹏.我国玉米主产区秸秆资源利用现状及其效果[J].应用生态学报,2011,22(6):1504-1510.Wang R F,Zhang J W,Dong S T,Liu P.Present situation of maize Sresource utilization and its effect in main maize production regions of China[J].Chinese Journal of Applied Ecology,2011,22(6):1504-1510.
[16]郝晓晖,胡荣桂,吴金水,等.长期施肥对稻田土壤有机氮、微生物生物量及功能多样性的影响[J].应用生态学报,2010,21(6):1477-1484.Hao X H,Hu R G,Wu J S,et al.Effects of long-term fertilization on paddy soils organic nitrogen,microbial biomass,and microbial functional diversity[J].Chinese Journal of Applied Ecology,2010,21(6):1477-1484.
[17]Gentile R,Vanlauwe B,Chivenge P,Six J.Trade-offs between the short-and long-term effects of residue quality on soil C and Ndynamics[J].Plant and Soil,2011,338:159-169.
[18]Brunetto G,Ventura M,Scandellari F,et al.Nutrient release during the decomposition of mowed perennial ryegrass and white clover and its contribution to nitrogen nutrition of grapevine[J].Nutrient Cycling in Agroecosystems,2011,90:299-308.
[19]Partey S T,Quashie-Sam S J,Thevathasan N V,Gordon A M.Decomposition and nutrient release patterns of the leaf biomass of the wild sunflower(Tithonia diversifolia):a comparative study with four leguminous agroforestry species[J].Agroforesty Systems,2011,81:123-134.
[20]Malhi S S,Nyborg M,Goddard T,Puurveen D.Long-term tillage,Sand N rate effects on quantity and quality of organic C and N in a Gray Luvisol soil[J].Nutrient Cycling in Agroecosystems,2011,90:1-20.
[21]Liang Q,Chen H Q,Gong Y S,et al.Effects of 15 years of manure and inorganic fertilizers on soil organic carbon fractions in a wheatmaize system in the North China Plain[J].Nutrient Cycling in Agroecosystems,2012,92:21-33.
[22]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,1999.Lu R K.Analytical methods for soil and agro-chemistry[M].Beijing:China Agricultural Science and Technology Press,1999.
[23]化党领,张诗卉,王瑞,等.施氮量对植烟土壤不同土层无机氮质量含量的调控[J].中国水土保持科学,2012,10(6):86-91.Hua D L,Zhang S H,Wang R,et al.Regulation of soil inorganic nitrogen by applying different nitrogen rate on flue-cured tobaccoplanting soil[J].Science of Soil and Water Conservation,2012,10(6):86-91.
[24]周建斌,李生秀.碱性过硫酸钾氧化法测定溶液中全氮含量氧化剂的选择[J].植物营养与肥料学报,1998,4(3):299-304.Zhou J B,Li S X.Choosing of a proper oxidizer for alkaline persulfate oxidation to determining total nitrogen in solution[J].Plant Nutrition and Fertilizer Science,1998,4(3):299-304.
[25]靳红梅,常志州,郭德杰,吴华山.追施猪粪沼液对菜地氨挥发的影响[J].土壤学报,2012,49(1):86-95.Jin H M,Chang Z Z,Guo D J,Wu H S.Effect of topdressing with digested pig slurry on ammonia volatilization in vegetable fields[J].Acta Pedologica Sinica,2012,49(1):86-95.
[26]Joergensen R G.The fumigation-extraction method to estimate soil microbial biomass:calibration of the k EC value[J].Soil Biology&Biochemistry,1996,28(1):25-31.
[27]Joergensen R G,Mueller T.The fumigation-extraction method to estimate soil microbial biomass:calibration of the k EN value[J].Soil Biology&Biochemistry,1996,28(1):33-37.
[28]Wu J,Joergensen R G,Pommerening B,et al.Measurement of soil microbial biomass C by fumigation-extraction-an automated procedure[J].Soil Biology&Biochemistry,1990,22(8):1167-1169.
[29]何振立.土壤微生物量的测定方法:现状和展望[J].土壤学进展,1994,22(4):36-44.He Z L.The measurement method of soil microbial:the present situation and prospect[J].Progress in Soil Science,1994,22(4):36-44.
[30]顾明华,区惠平,刘昔辉,等.不同耕作方式下稻田土壤的氮素形态及氮素转化菌特征[J].应用生态学报,2009,20(6):1362-1368.Gu M H,Ou H P,Liu X H,et al.Characteristics of N forms and N-transforming bacteria in paddy soil under different tillage patterns[J].Chinese Journal of Applied Ecology,2009,20(6):1362-1368.
[31]Yoshida T,Benjamin C P.Transformation of soil and fertilizer nitrogen in paddy soil and their availability to rice plants[J].Plant and Soil,1997,47:113-123.
[32]李玉英,孙建好,余常兵,等.施氮量和蚕豆/玉米间作对土壤无机氮时空分布的影响[J].植物营养与肥料学报,2009,15(4):815-823.Li Y Y,Sun J H,Yu C B,et al.Effects of nitrogen fertilization application and faba bean/maize intercropping on the spatial and temporal distribution of soil inorganic nitrogen[J].Plant Nutrition and Fertilizer Science,2009,15(4):815-823.
[33]武际,郭熙盛,鲁剑巍,等.连续秸秆覆盖对土壤无机氮供应特征和作物产量的影响[J].中国农业科学,2012,45(9):1741-1749.Wu J,Guo X S,Lu J W,et al.Effects of continuous straw mulching on supply characteristics of soil inorganic nitrogen and crop yields[J].Scientia Agricultura Sinica,2012,45(9):1741-1749.
[34]熊淑萍,车芳芳,马新明,等.氮肥形态对冬小麦根际土壤氮素生理群活性及无机氮含量的影响[J].生态学报,2012,32(16):5138-5145.Xiong S P,Che F F,Ma X M,et al.Effects of nitrogen form on the activity of nitrogen bacteria group and inorganic nitrogen in rhizosphere soil of winter wheat[J].Acta Ecologica Sinica,2012,32(16):5138-5145.
[35]王淑平,周广胜,姜亦梅,等.玉米植株残体留田对土壤生化环境因子的影响[J].吉林农业大学学报,2002,24(6):54-57.Wang S P,Zhou G S,Jiang Y M,et al.Effect of corn stalk and stubble remained in field on soil biochemical factors[J].Journal of Jilin Agricultural University,2002,24(6):54-57.
[36]强学彩.秸秆还田量的农田生态效应研究[D].北京:中国农业大学硕士学位论文,2003.Qiang X C.Study on ecological effect in farm system of different amount of crop residues incorporation[D].Beijing:MS Thesis of China Agricultural University,2003.
[37]B?hme L,Langer U,B?hme F.Microbial biomass,enzyme activities and microbial community structure in two European long-term field experiments[J].Agriculture,Ecosystems&Environment,2005,(109):141-152.
[38]代静玉,周江敏,秦淑平.几种有机物料分解过程中溶解性有机物质化学成分的变化[J].土壤通报,2004,35(6):724-727.Dai J Y,Zhou J M,Qin S P.Dynamic changes of chemical composition of dissolved organic matter during decomposition of organic materials[J].Chinese Journal of Soil Science,2004,35(6):724-727.
[39]杨绒,严德翼,周建斌,等.黄土区不同类型土壤可溶性有机氮的含量及特性[J].生态学报,2007,27(4):1397-1403.Yang R,Yan D Y,Zhou J B,et al.Soluble organic nitrogen(SON)in different soils on the loess Plateau of China[J].Acta Ecologica Sinica,2007,27(4):1397-1403.
[2]赵士诚,曹彩云,李科江,等.长期秸秆还田对华北潮土肥力、氮库组分及作物产量的影响[J].植物营养与肥料学报,2014,20(6):1441-1449.Zhao S C,Cao C Y,Li K J,et al.Effects of long-term S return on soil fertility,nitrogen pool fractions and crop yields on a fluvo-aquic soil in North China[J].Journal of Plant Nutrition and Fertilizer,2014,20(6):1441-1449.
[3]赵俊晔,于振文,李延奇,王雪.施氮量对土壤无机氮分布和微生物量氮含量及小麦产量的影响[J].植物营养与肥料学报,2006,12(4):466-472.Zhao J Y,Yu Z W,Li Y Q,Wang X.Effects of nitrogen application rate on soil inorganic nitrogen distribution,microbial biomass nitrogen content and yield of wheat[J].Plant Nutrition and Fertilizer Science,2006,12(4):466-472.
[4]Yan D Z,Wang D J,Yang L Z.Long-term effect of chemical fertilizer,S,and manure on labile organic matter fractions in a paddy soil[J].Biology and Fertility of Soils,2007,44:93-101.
[5]Gardenas A I,Agren G I,Bird J A,et al.Knowledge gaps in soil carbon and nitrogen interactions-From molecular to global scale[J].Soil Biology&Biochemistry,2011,43(4):702-717.
[6]Soper F M,Paungfoo-Lonhienne C,Brackin R,Rentsch D,et al.Arabidopsis and Lobelia anceps access small peptides as a nitrogen source for growth[J].Functional Plant Biology,2011,38(10):788-796.
[7]Van Kessel C,Clough T,van Groenigen J W.Dissolved organic nitrogen:An overlooked pathway of nitrogen loss from agricultural systems[J].Journal of Environmental Quality,2009,38(2):393-401.
[8]Park J H,Kalbitz K,Matzner E.Resource control on the production of dissolved organic carbon and nitrogen in a deciduous forest floor[J].Soil Biology&Biochemistry,2002,34:813-822.
[9]Hedin L O,Armesto J J,Johnson A H.Patterns of nutrient loss from unpolluted,old-growth temperate forests:Evaluation of biogeochemical theory[J].Ecology,1995,76(2):493-509.
[10]Quaills R G,Haines B L,Swank W T.Fluxes of dissolved organic nutrients and humic substances in a deciduous forest[J].Ecology,1991,72(1):254-266.
[11]李贵桐,张宝贵,李保国.秸秆预处理对土壤微生物量及呼吸活性的影响[J].应用生态学报,2003,14(12):2225-2228.Li G T,Zhang B G,Li B G.Effect of S pretreatment on soil microbial biomass and respiration activity[J].Chinese Journal of Applied Ecology,2003,14(12):2225-2228.
[12]练成燕,张桃林,王兴祥.有机物料对红壤几种形态碳氮及酸度的影响[J].中国农业科学,2009,42(11):3922-3932.Lian C Y,Zhang T L,Wang X X.Effects of organic materials on several forms of soil carbon and nitrogen and soil acidity[J].Scientia Agricultura Sinica,2009,42(11):3922-3932.
[13]Recous S,Robin D,Darwis D,et al.Soil inorganic N availability:Effect on maize residue decomposition[J].Soil Biology&Biochemistry,1995,27(12):1529-1538.
[14]朱兆良,金继运.保障我国粮食安全的肥料问题[J].植物营养与肥料学报,2013,19(2):259-273.Zhu Z L,Jin J Y.Fertilizer use and food security in China[J].Plant Nutrition and Fertilizer Science,2013,19(2):259-273.
[15]王如芳,张吉旺,董树亭,刘鹏.我国玉米主产区秸秆资源利用现状及其效果[J].应用生态学报,2011,22(6):1504-1510.Wang R F,Zhang J W,Dong S T,Liu P.Present situation of maize Sresource utilization and its effect in main maize production regions of China[J].Chinese Journal of Applied Ecology,2011,22(6):1504-1510.
[16]郝晓晖,胡荣桂,吴金水,等.长期施肥对稻田土壤有机氮、微生物生物量及功能多样性的影响[J].应用生态学报,2010,21(6):1477-1484.Hao X H,Hu R G,Wu J S,et al.Effects of long-term fertilization on paddy soils organic nitrogen,microbial biomass,and microbial functional diversity[J].Chinese Journal of Applied Ecology,2010,21(6):1477-1484.
[17]Gentile R,Vanlauwe B,Chivenge P,Six J.Trade-offs between the short-and long-term effects of residue quality on soil C and Ndynamics[J].Plant and Soil,2011,338:159-169.
[18]Brunetto G,Ventura M,Scandellari F,et al.Nutrient release during the decomposition of mowed perennial ryegrass and white clover and its contribution to nitrogen nutrition of grapevine[J].Nutrient Cycling in Agroecosystems,2011,90:299-308.
[19]Partey S T,Quashie-Sam S J,Thevathasan N V,Gordon A M.Decomposition and nutrient release patterns of the leaf biomass of the wild sunflower(Tithonia diversifolia):a comparative study with four leguminous agroforestry species[J].Agroforesty Systems,2011,81:123-134.
[20]Malhi S S,Nyborg M,Goddard T,Puurveen D.Long-term tillage,Sand N rate effects on quantity and quality of organic C and N in a Gray Luvisol soil[J].Nutrient Cycling in Agroecosystems,2011,90:1-20.
[21]Liang Q,Chen H Q,Gong Y S,et al.Effects of 15 years of manure and inorganic fertilizers on soil organic carbon fractions in a wheatmaize system in the North China Plain[J].Nutrient Cycling in Agroecosystems,2012,92:21-33.
[22]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,1999.Lu R K.Analytical methods for soil and agro-chemistry[M].Beijing:China Agricultural Science and Technology Press,1999.
[23]化党领,张诗卉,王瑞,等.施氮量对植烟土壤不同土层无机氮质量含量的调控[J].中国水土保持科学,2012,10(6):86-91.Hua D L,Zhang S H,Wang R,et al.Regulation of soil inorganic nitrogen by applying different nitrogen rate on flue-cured tobaccoplanting soil[J].Science of Soil and Water Conservation,2012,10(6):86-91.
[24]周建斌,李生秀.碱性过硫酸钾氧化法测定溶液中全氮含量氧化剂的选择[J].植物营养与肥料学报,1998,4(3):299-304.Zhou J B,Li S X.Choosing of a proper oxidizer for alkaline persulfate oxidation to determining total nitrogen in solution[J].Plant Nutrition and Fertilizer Science,1998,4(3):299-304.
[25]靳红梅,常志州,郭德杰,吴华山.追施猪粪沼液对菜地氨挥发的影响[J].土壤学报,2012,49(1):86-95.Jin H M,Chang Z Z,Guo D J,Wu H S.Effect of topdressing with digested pig slurry on ammonia volatilization in vegetable fields[J].Acta Pedologica Sinica,2012,49(1):86-95.
[26]Joergensen R G.The fumigation-extraction method to estimate soil microbial biomass:calibration of the k EC value[J].Soil Biology&Biochemistry,1996,28(1):25-31.
[27]Joergensen R G,Mueller T.The fumigation-extraction method to estimate soil microbial biomass:calibration of the k EN value[J].Soil Biology&Biochemistry,1996,28(1):33-37.
[28]Wu J,Joergensen R G,Pommerening B,et al.Measurement of soil microbial biomass C by fumigation-extraction-an automated procedure[J].Soil Biology&Biochemistry,1990,22(8):1167-1169.
[29]何振立.土壤微生物量的测定方法:现状和展望[J].土壤学进展,1994,22(4):36-44.He Z L.The measurement method of soil microbial:the present situation and prospect[J].Progress in Soil Science,1994,22(4):36-44.
[30]顾明华,区惠平,刘昔辉,等.不同耕作方式下稻田土壤的氮素形态及氮素转化菌特征[J].应用生态学报,2009,20(6):1362-1368.Gu M H,Ou H P,Liu X H,et al.Characteristics of N forms and N-transforming bacteria in paddy soil under different tillage patterns[J].Chinese Journal of Applied Ecology,2009,20(6):1362-1368.
[31]Yoshida T,Benjamin C P.Transformation of soil and fertilizer nitrogen in paddy soil and their availability to rice plants[J].Plant and Soil,1997,47:113-123.
[32]李玉英,孙建好,余常兵,等.施氮量和蚕豆/玉米间作对土壤无机氮时空分布的影响[J].植物营养与肥料学报,2009,15(4):815-823.Li Y Y,Sun J H,Yu C B,et al.Effects of nitrogen fertilization application and faba bean/maize intercropping on the spatial and temporal distribution of soil inorganic nitrogen[J].Plant Nutrition and Fertilizer Science,2009,15(4):815-823.
[33]武际,郭熙盛,鲁剑巍,等.连续秸秆覆盖对土壤无机氮供应特征和作物产量的影响[J].中国农业科学,2012,45(9):1741-1749.Wu J,Guo X S,Lu J W,et al.Effects of continuous straw mulching on supply characteristics of soil inorganic nitrogen and crop yields[J].Scientia Agricultura Sinica,2012,45(9):1741-1749.
[34]熊淑萍,车芳芳,马新明,等.氮肥形态对冬小麦根际土壤氮素生理群活性及无机氮含量的影响[J].生态学报,2012,32(16):5138-5145.Xiong S P,Che F F,Ma X M,et al.Effects of nitrogen form on the activity of nitrogen bacteria group and inorganic nitrogen in rhizosphere soil of winter wheat[J].Acta Ecologica Sinica,2012,32(16):5138-5145.
[35]王淑平,周广胜,姜亦梅,等.玉米植株残体留田对土壤生化环境因子的影响[J].吉林农业大学学报,2002,24(6):54-57.Wang S P,Zhou G S,Jiang Y M,et al.Effect of corn stalk and stubble remained in field on soil biochemical factors[J].Journal of Jilin Agricultural University,2002,24(6):54-57.
[36]强学彩.秸秆还田量的农田生态效应研究[D].北京:中国农业大学硕士学位论文,2003.Qiang X C.Study on ecological effect in farm system of different amount of crop residues incorporation[D].Beijing:MS Thesis of China Agricultural University,2003.
[37]B?hme L,Langer U,B?hme F.Microbial biomass,enzyme activities and microbial community structure in two European long-term field experiments[J].Agriculture,Ecosystems&Environment,2005,(109):141-152.
[38]代静玉,周江敏,秦淑平.几种有机物料分解过程中溶解性有机物质化学成分的变化[J].土壤通报,2004,35(6):724-727.Dai J Y,Zhou J M,Qin S P.Dynamic changes of chemical composition of dissolved organic matter during decomposition of organic materials[J].Chinese Journal of Soil Science,2004,35(6):724-727.
[39]杨绒,严德翼,周建斌,等.黄土区不同类型土壤可溶性有机氮的含量及特性[J].生态学报,2007,27(4):1397-1403.Yang R,Yan D Y,Zhou J B,et al.Soluble organic nitrogen(SON)in different soils on the loess Plateau of China[J].Acta Ecologica Sinica,2007,27(4):1397-1403.