中药复方提取物对免疫抑制雏鸡IBD疫苗的免疫增强效果
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摘要
为探索中药复方提取物(由黄芪、女贞子、枸杞子、菟丝子按质量比2∶2∶1∶1制备而成)对免疫抑制雏鸡的免疫增强效果,将180羽3日龄SPF雏鸡随机均分为对照组、模型组、阳性药物组、中药复方提取物高、中、低剂量组,试验组雏鸡连续3 d每天肌肉注射环磷酰胺90 mg/kg,建立鸡免疫抑制模型,对照组注射等量的生理盐水,中药复方提取物高、中、低组每天分别在饮水中添加10.0、5.0、2.5g/L的中药复方提取物,阳性药物组每天添加200 mg/L的黄芪多糖粉,连续添加7 d。试验期38 d。结果显示:饮水中添加10.0、5.0 g/L中药复方提取物,免疫抑制雏鸡末体质量、日均增体质量和脾脏指数均显著高于模型组的(P<0.05);添加14 d后,雏鸡血清IgM含量开始高于对照组,并维持在较高水平;21 d后,血清中IgA、IFN–γ和中剂量组的IgG含量显著高于模型组的(P<0.05);在接种传染性法氏囊(IBD)疫苗后7 d,雏鸡传染性法氏囊病毒抗体效价提高,接种14 d后,还能维持较高的抗体效价水平,IBD疫苗的免疫效果增强。可见,在本试验条件下,在饮水中添加10.0、5.0 g/L的中药复方提取物能有效提高免疫抑制雏鸡的生长性能,改善其免疫功能,增强IBD疫苗的免疫保护效果。
To explore the immunological enhancing effect of traditional Chinese medicine compound extract made by ASTRAGALI RADIX, LIGUSTRI LUCIDI FRUCTUS, LYCII FRUCTUS AND CUSCUTAE SEMEN on immunosuppressed chicks. 180 SPF chicks of 3-day age were randomly divided into control group, model group, positive drug group, traditional Chinese medicine extract high, medium and low dose groups. The test groups' chicks were established immunosuppressed chicks model by intramuscular injection with cyclophosphamide of 90 mg/kg for 3 consecutive days, and the control group was given equivalent physiological saline by the same way. Then, the traditional Chinese medicine extract high, medium and low dose groups were respectively added the medicine in drinking water of10.0, 5.0, 2.5 g/L per day, and the positive drug group was added to astragalus polysaccharide powder of 200 mg/L per day for 7 consecutive days, the test period was 38 days. The results showed that the immunosuppressed chicks were added to the extract of 10.0 and 5.0 g/L could significant improve their final weight, daily gain and spleen index compared with model group(P<0.05). After 14 days of drug addition, serum IgM content began to be higher than that of the control group and maintained at a high level. After 21 days, the levels of serum IgA, IFN-γ and IgG of the medium dose group were significantly higher than the model group(P<0.05). Meanwhile, the antibody titer was increased after 7 days of inoculation of the infectious bursal disease(IBD) vaccine, and the high antibody titer level was maintained after14 days of inoculation, which enhanced the immune effect of the IBD vaccine.These results indicated that the traditional Chinese medicine extract with the dose of 10.0 g/L and 5.0 g/L in drinking water could effectively enhance the growth performance of immunosuppressed chicks, their immune function, and the immune protection effect of IBD vaccine.
To explore the immunological enhancing effect of traditional Chinese medicine compound extract made by ASTRAGALI RADIX, LIGUSTRI LUCIDI FRUCTUS, LYCII FRUCTUS AND CUSCUTAE SEMEN on immunosuppressed chicks. 180 SPF chicks of 3-day age were randomly divided into control group, model group, positive drug group, traditional Chinese medicine extract high, medium and low dose groups. The test groups' chicks were established immunosuppressed chicks model by intramuscular injection with cyclophosphamide of 90 mg/kg for 3 consecutive days, and the control group was given equivalent physiological saline by the same way. Then, the traditional Chinese medicine extract high, medium and low dose groups were respectively added the medicine in drinking water of10.0, 5.0, 2.5 g/L per day, and the positive drug group was added to astragalus polysaccharide powder of 200 mg/L per day for 7 consecutive days, the test period was 38 days. The results showed that the immunosuppressed chicks were added to the extract of 10.0 and 5.0 g/L could significant improve their final weight, daily gain and spleen index compared with model group(P<0.05). After 14 days of drug addition, serum IgM content began to be higher than that of the control group and maintained at a high level. After 21 days, the levels of serum IgA, IFN-γ and IgG of the medium dose group were significantly higher than the model group(P<0.05). Meanwhile, the antibody titer was increased after 7 days of inoculation of the infectious bursal disease(IBD) vaccine, and the high antibody titer level was maintained after14 days of inoculation, which enhanced the immune effect of the IBD vaccine.These results indicated that the traditional Chinese medicine extract with the dose of 10.0 g/L and 5.0 g/L in drinking water could effectively enhance the growth performance of immunosuppressed chicks, their immune function, and the immune protection effect of IBD vaccine.
引文
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[11]许丹妮,马玉芳,李健,等.金线莲多糖对小鼠免疫功能的影响[J].福建农林大学学报(自然科学版),2013,42(5):536-538.
[12]廖吕燕.“芪苓”制剂多糖对免疫抑制小鼠肠道黏膜免疫及免疫调节作用的影响[D].福州:福建农林大学,2010.
[13]WILSON M R,VAN RAVENSTEIN E,MILLER N W,et al.cDNA sequences and organization of IgM heavy chain genes in two holostean fish[J].Developmental&Comparative Immunology,1995,19(2):153-164.
[14]章振华,李林,景小冬,等.鸡新城疫-传染性法氏囊病-禽流感(H9亚型)三联灭活疫苗对雏鸡的免疫效力研究[J].动物医学进展,2018,39(6):6-10.
[2]黄鑫,高亚男,王加启,等.霉菌毒素对肠道黏蛋白的影响及其作用机制[J].动物营养学报,2018,30(2):476-483.
[3]孙永波,王亚,萨仁娜,等.舍内空气质量对肉鸡健康影响的研究进展[J].动物营养学报,2018,30(4):1230-1237.
[4]吕其壮,林谦,陈旭健.广西地区陆川猪病毒性免疫抑制病的流行病学调查[J].中国预防兽医学报,2018,40(2):106-111.
[5]廖洁丹,陈玉明,张济培.鸡传染性法氏囊病病毒的分子致病机理研究进展[J].中国家禽,2018,40(5):1-5.
[6]沙莎,宋振辉.动物微生物实验教程[M].重庆:西南师范大学出版社,2011:110-113.
[7]陶浩,魏炳栋,陈群.黄芪多糖对肉仔鸡生长性能和脏器指数及胰腺消化酶活性的影响[J].湖南农业大学学报(自然科学版),2011,37(4):425-428.
[8]邱蓉丽,李磷.中药女贞子化学与药理研究进展[J].中药材,2007,30(7):891-894.
[9]朱燕飞.枸杞子药理作用概述[J].浙江中西医结合杂志,2005,15(5):322-323.
[10]王水莲,刘进辉,孙志良,等.不同时期鸡法氏囊与脾脏的组织学观察[J].湖南农业大学学报(自然科学版),2005,31(2):199-202.
[11]许丹妮,马玉芳,李健,等.金线莲多糖对小鼠免疫功能的影响[J].福建农林大学学报(自然科学版),2013,42(5):536-538.
[12]廖吕燕.“芪苓”制剂多糖对免疫抑制小鼠肠道黏膜免疫及免疫调节作用的影响[D].福州:福建农林大学,2010.
[13]WILSON M R,VAN RAVENSTEIN E,MILLER N W,et al.cDNA sequences and organization of IgM heavy chain genes in two holostean fish[J].Developmental&Comparative Immunology,1995,19(2):153-164.
[14]章振华,李林,景小冬,等.鸡新城疫-传染性法氏囊病-禽流感(H9亚型)三联灭活疫苗对雏鸡的免疫效力研究[J].动物医学进展,2018,39(6):6-10.
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