沈阳市汉族与满族女性5,10-亚甲基四氢叶酸还原酶、甲硫氨酸合成酶还原酶基因的单核苷酸多态性分布特征
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摘要
目的探讨沈阳市汉族与满族女性中5,10-亚甲基四氢叶酸还原酶(MTHFR)及甲硫氨酸合成酶还原酶(MTRR)基因的单核苷酸多态性分布特征并与其他少数民族做区域比较,为进一步群体遗传分析奠定基础。方法选取2015年4月-2017年7月在沈阳共济爱婴医院进行体检及孕期检查的汉族和满族女性作为研究对象,其中汉族469例,满族67例,检测其MTHFR C677T、A1298C和MTRR A66G基因位点多态性,统计分析该地区基因多态性的分布特征,并与已报道的其他地区少数民族女性数据进行比较。结果沈阳市汉族与满族女性比较,MTHFR C677T、A1298C和MTRR A66G差异无统计学意义(均P>0. 05)。武阳市满族女性和其他地区少数民族女性比较,MTHFR C677T的基因型频率与延边朝鲜族、银川回族、张家界土家族、思南苗族、柳州壮族、三亚黎族女性差异有统计学意义(均P<0. 05);而MTHFR A1298C的基因型频率与柳州壮族和三亚黎族女性差异有统计学意义(均P<0. 05); MTRR A66G的分布上则与乌鲁木齐维吾尔族、三亚黎族女性差异有统计学意义(均P<0. 05)。结论沈阳市汉族女性和满族女性的MTHFR和MTRR基因多态性分布特征差异无统计学意义,可能与两个民族同化和融合有关;沈阳地区满族和汉族女性与其他地区少数民族女性相比差异存在统计学意义。
Objective To explore the distribution characteristics of single nucleotide polymorphisms of 5,10-methylenetetrahydrofolate reductase( MTHFR) and methionine synthase reductase( MTRR) among Han women and Manchu women in Shenyang,compare the results with reported data of women of other ethnic minorities in other regions,lay a foundation for further population genetic analysis. Methods A total of 469 Han women and 67 Manchu women receiving physical examination and prenatal examination in Shenyang Gongji Maternity Hospital from April 2015 to July 2017 were selected as study object,MTHFR C677 T,A1298 C and MTRR A66G gene polymorphisms were detected. The distribution characteristics of gene polymorphisms in Shenyang were analyzed statistically,then the results were compared with reported data of women of other ethnic minorities in other regions. Results There was no statistically significant difference in the distribution characteristics of MTHFR C677 T,A1298 C and MTRR A66G gene polymorphisms between Han women and Manchu women in Shenyang( P>0. 05). There were statistically significant differences in MTHFR C677 T genotype frequency between Manchu women in Shenyang and Korean women in Yanbian,Hui women in Yinchuan,Tujia women in Zhangjiajie,Miao women in Sinan,Zhuang women in Liuzhou,Li women in Sanya( P<0. 05). There were statistically significant differences in MTHFR A1298 C T genotype frequency between Manchu women in Shenyang and Zhuang women in Liuzhou,Li women in Sanya( P < 0. 05). There were statistically significant differences in MTRR A66G genotype frequency between Manchu women in Shenyang and Uygur women in Urumqi,Li women in Sanya( P < 0. 05).Conclusion There is no statistically significant difference in distribution characteristics of gene polymorphisms of MTHFR and MTRR between Han women and Manchu women in Shenyang,which is correlated with national assimilation and fusion,compared with the women of other ethnic minorities in other regions,there are statistically significant differences.
Objective To explore the distribution characteristics of single nucleotide polymorphisms of 5,10-methylenetetrahydrofolate reductase( MTHFR) and methionine synthase reductase( MTRR) among Han women and Manchu women in Shenyang,compare the results with reported data of women of other ethnic minorities in other regions,lay a foundation for further population genetic analysis. Methods A total of 469 Han women and 67 Manchu women receiving physical examination and prenatal examination in Shenyang Gongji Maternity Hospital from April 2015 to July 2017 were selected as study object,MTHFR C677 T,A1298 C and MTRR A66G gene polymorphisms were detected. The distribution characteristics of gene polymorphisms in Shenyang were analyzed statistically,then the results were compared with reported data of women of other ethnic minorities in other regions. Results There was no statistically significant difference in the distribution characteristics of MTHFR C677 T,A1298 C and MTRR A66G gene polymorphisms between Han women and Manchu women in Shenyang( P>0. 05). There were statistically significant differences in MTHFR C677 T genotype frequency between Manchu women in Shenyang and Korean women in Yanbian,Hui women in Yinchuan,Tujia women in Zhangjiajie,Miao women in Sinan,Zhuang women in Liuzhou,Li women in Sanya( P<0. 05). There were statistically significant differences in MTHFR A1298 C T genotype frequency between Manchu women in Shenyang and Zhuang women in Liuzhou,Li women in Sanya( P < 0. 05). There were statistically significant differences in MTRR A66G genotype frequency between Manchu women in Shenyang and Uygur women in Urumqi,Li women in Sanya( P < 0. 05).Conclusion There is no statistically significant difference in distribution characteristics of gene polymorphisms of MTHFR and MTRR between Han women and Manchu women in Shenyang,which is correlated with national assimilation and fusion,compared with the women of other ethnic minorities in other regions,there are statistically significant differences.
引文
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[2]张婷,谢渊,李毅,等.贵州3个民族亚甲基四氢叶酸还原酶基因的遗传多态性[J].重庆医学,2013,28(42):3413-3415.
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[5]郝友瑛,赵红,鲁衍强,等.新疆维吾尔族和汉族女性MTHFR和MTRR基因单核苷酸多态性比较[J].中国妇幼保健,2014,29(21):3495-3497.
[6]杨玉清,唐菊香,鲁衍强,等.银川市回族和汉族女性MTHFR和MTRR基因单核苷酸多态性分析等[J].中国妇幼保健,2015,30(35):6267-6269.
[7]符春翠,鲁衍强,李瑛,等.张家界市土家族与汉族女性MTH-FR、MTRR基因型和等位基因频率分布研究及与其他地区比较[J].中国妇幼保健,2016,31(9):1898-1901.
[8]毛万成,鲁衍强,薛琰,等.贵州思南县汉族与苗族、土家族女性MTHFR、MTRR基因多态性研究[J].中国计划生育学杂志,2015,24(5):310-313.
[9]邱萍,鲁衍强,付敏,等.柳州市壮族与汉族女性5,10-亚甲基四氢叶酸还原酶、甲硫氨酸合成酶还原酶基因型和等位基因频率的分布[J].生殖与避孕,2016,36(7):569-575.
[10]谭蓝,鲁衍强,李瑛,等.三亚市汉族和黎族女性MTHFR与MTRR基因多态性研究[J].广东医学,2014,35(5月增刊):164-166.
[11]于长春,赵永斌,孙文怡.满族与中国东北地区各民族母系亲缘关系的遗传学证据[J].吉林农业,2012,(10):262-264.
[12]Cui H,Lu Y,Ma S,et al.Geographical distribution of MTHFRand MTRR gene polymorphisms among the Han women in Zhengzhou city[J].Zhong nan da xue xue bao Yi xue ban=Journal of Central South University Medical sciences,2015,40(7):710-714.
[13]Yang B,Liu Y,Li Y,et al.Geographical distribution of MTHFRC677T,A1298C and MTRR A66G gene polymorphisms in China:findings from 15357 adults of Han nationality[J].PloS one,2013,8(3):e57917.
[14]温春梅.中国少数民族地区妇幼保健服务提供模式研究[D].华中科技大学,2011.
[2]张婷,谢渊,李毅,等.贵州3个民族亚甲基四氢叶酸还原酶基因的遗传多态性[J].重庆医学,2013,28(42):3413-3415.
[3]方园,鲁衍强,薛琰等.九江市汉族女性MTHFR、MTRR基因型和等位基因频率分布与其他地区汉族女性的比较[J].中国妇幼保健,2015,30(11):1732-1735.
[4]于延辉,鲁衍强,李瑛等.延边朝鲜族自治州汉族女性和朝鲜族女性MTHFR与MTRR基因多态性分布研究[J].实用医学杂志,2014,30(5月增刊):7-9.
[5]郝友瑛,赵红,鲁衍强,等.新疆维吾尔族和汉族女性MTHFR和MTRR基因单核苷酸多态性比较[J].中国妇幼保健,2014,29(21):3495-3497.
[6]杨玉清,唐菊香,鲁衍强,等.银川市回族和汉族女性MTHFR和MTRR基因单核苷酸多态性分析等[J].中国妇幼保健,2015,30(35):6267-6269.
[7]符春翠,鲁衍强,李瑛,等.张家界市土家族与汉族女性MTH-FR、MTRR基因型和等位基因频率分布研究及与其他地区比较[J].中国妇幼保健,2016,31(9):1898-1901.
[8]毛万成,鲁衍强,薛琰,等.贵州思南县汉族与苗族、土家族女性MTHFR、MTRR基因多态性研究[J].中国计划生育学杂志,2015,24(5):310-313.
[9]邱萍,鲁衍强,付敏,等.柳州市壮族与汉族女性5,10-亚甲基四氢叶酸还原酶、甲硫氨酸合成酶还原酶基因型和等位基因频率的分布[J].生殖与避孕,2016,36(7):569-575.
[10]谭蓝,鲁衍强,李瑛,等.三亚市汉族和黎族女性MTHFR与MTRR基因多态性研究[J].广东医学,2014,35(5月增刊):164-166.
[11]于长春,赵永斌,孙文怡.满族与中国东北地区各民族母系亲缘关系的遗传学证据[J].吉林农业,2012,(10):262-264.
[12]Cui H,Lu Y,Ma S,et al.Geographical distribution of MTHFRand MTRR gene polymorphisms among the Han women in Zhengzhou city[J].Zhong nan da xue xue bao Yi xue ban=Journal of Central South University Medical sciences,2015,40(7):710-714.
[13]Yang B,Liu Y,Li Y,et al.Geographical distribution of MTHFRC677T,A1298C and MTRR A66G gene polymorphisms in China:findings from 15357 adults of Han nationality[J].PloS one,2013,8(3):e57917.
[14]温春梅.中国少数民族地区妇幼保健服务提供模式研究[D].华中科技大学,2011.
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