30个多穗柯种源主要经济性状及活性成分分析与评价
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摘要
【目的】探究30个多穗柯种源主要经济性状和活性成分的差异,为筛选多穗柯优良种质资源提供理论依据。【方法】采用田间随机区组试验结合方差和主成分分析,对6个省(区、直辖市)30个多穗柯种源苗木的苗高、地径、产量、根皮苷含量、三叶苷含量进行测定。【结果】30个多穗柯种源的树高、产量、老叶根皮苷含量和嫩叶三叶苷含量的种间差异性均达到显著性水平(P<0.05)。对30个多穗柯种源的5个指标进行主成分分析,将其转化为3个主成分,这3个主成分可以代表原本5个指标的82.541%的综合信息,通过综合评价,CQLP2的综合评分(F值)最高为1.420094,其第1主成分的得分也最高,说明其综合指标和经济性状表现最优;GXYJ13的综合评分(F值)排名第2为0.888433,其第2主成分的得分最高,但第1主成分得分为负值(-0.57666),说明其综合评价最优,但经济性状表现比较差,综合得分不如CQLP2。【结论】综合评价最优的10个品种为CQLP2、GXYJ13、GXTL5、GXNP3、CQLP4、GXTL6、GXYJ11、GXDB5、CQLP1和CQJYS2。
【Objective】To provide a theoretical basis for screeningfine germplasm resource, differences in main economic traits and active constituents of thirty Lithocarpus ploystachyus Rehd.provenances were studied.【Method】The differences of characters were studied by field randomized block test combining analysis of variance and principal component regression, which included seedling height, ground diameter, yield, phloridzin content and trilobatin content of thirty Lithocarpus ploystachyus Rehd.provenances from six provinces(region and municipal). 【Result】Seedling height, ground diameter, yield, phloridzin content and trilobatin content of thirty Lithocarpus ploystachyus Rehd.were significantly different(P<0.05). Five indexes transformed into three principal components by principal component analysis that can represent the 82.542 % comprehensive information of the originalfive indicators. According to synthetically evaluation, comprehensive mark and the score of the first principal component of CQLP2 were the highest(F =1.420094), which showed that aggregative indicator and the economic characters were the best.Comprehensive score of GXYJ13 ranked second and the score of the second principal component were the highest, but the first principal component scored negative(-0.57666), which showed that the comprehensive evaluation was the best, but the economic performance was poor. Composite scores of GXYJ13 was below that of CQLP2. 【Conclusion】The ten varieties with the best comprehensive evaluation were CQLP2, GXYJ13, GXTL5, GXNP3, CQLP4, GXTL6, GXYJ11, GXDB5, CQLP1 and CQJYS2.
【Objective】To provide a theoretical basis for screeningfine germplasm resource, differences in main economic traits and active constituents of thirty Lithocarpus ploystachyus Rehd.provenances were studied.【Method】The differences of characters were studied by field randomized block test combining analysis of variance and principal component regression, which included seedling height, ground diameter, yield, phloridzin content and trilobatin content of thirty Lithocarpus ploystachyus Rehd.provenances from six provinces(region and municipal). 【Result】Seedling height, ground diameter, yield, phloridzin content and trilobatin content of thirty Lithocarpus ploystachyus Rehd.were significantly different(P<0.05). Five indexes transformed into three principal components by principal component analysis that can represent the 82.542 % comprehensive information of the originalfive indicators. According to synthetically evaluation, comprehensive mark and the score of the first principal component of CQLP2 were the highest(F =1.420094), which showed that aggregative indicator and the economic characters were the best.Comprehensive score of GXYJ13 ranked second and the score of the second principal component were the highest, but the first principal component scored negative(-0.57666), which showed that the comprehensive evaluation was the best, but the economic performance was poor. Composite scores of GXYJ13 was below that of CQLP2. 【Conclusion】The ten varieties with the best comprehensive evaluation were CQLP2, GXYJ13, GXTL5, GXNP3, CQLP4, GXTL6, GXYJ11, GXDB5, CQLP1 and CQJYS2.
引文
[1]中国科学院中国植物志编辑委员会.中国植物志(第22卷)[M].北京:科学出版社,1977:201.
[2]中国科学院植物研究所.中国高等植物图鉴(第1册) [M].北京:科学出版社,1972:434.
[3]何春年,彭勇,肖伟,等.多穗柯甜茶的研究进展[J].时珍国医国药,2012,23(5):1253-1255.
[4]Dudash J,Zhang X,Zeck R,et al.Glycosylated dihydrochalcones as potent and selective sodium glucose co-transporter 2 (SGLT-2) inhibitors[J].Bioorganic & Medicinal Chemistry Letters,2004,14(20):5121-5125.
[5]董华强,宁正祥,于立静,等.多穗柯黄酮根皮苷对糖尿病小鼠的降血糖血脂效果[J].食品科学,2006,27(12):714-718.
[6]Yang W M,Liu J K,Qin X D,et al.Antioxidant activities of three dihydrochalcone glucosides from leaves of Lithocarpus pachyphyllus[J].Zeitschrift für Naturforschung C,2004,59(7-8):481-484.
[7]燕妮,王佐,高雄,等.多穗柯挥发性组分分析及抗癌活性的初步研究[J].现代食品科技,2017,33(3):35-39,73.
[8]李胜华,伍贤进,曾军英,等.多穗柯中黄酮类成分研究[J].中草药,2010,41(21):1967-1969.
[9]肖坤福,廖晓峰.多穗柯黄酮类化合物的分离及鉴定[J].林产化学与工业,2006,26(3):85-87.
[10]董华强,宁正祥,崔志新,等.微波辅助提取多穗柯嫩叶黄酮工艺研究[J].农业工程学报,2007,23(2):213-217.
[11]李胜华,伍贤进,杨青丹,等.多穗柯化学成分研究[J].中药材,2010,33(4):549-551.
[12]Chen Z H,Zhang R J,Wu J,et al.New dihydrochalcone glycosides from Lithocarpus litseifolius and the phenomenon of C-H C-D exchange observed in NMR spectra of phenolic components[J].Journal of Asian Natural Products Research,2009,11(6):508-513.
[13]李胜华,伍贤进,向晓军.多穗柯有效成分的提取及抑菌效果研究[J].食品科技,2010,35(3):211-214.
[14]单思聪,孙爽.超临界萃取多穗柯黄酮及其抗运动氧化功能研究[J].食品研究与开发,2016,37(20):67-70.
[15]Hou S Z,Chen S X,Huang S,et al.The hypoglycemic activity of Lithocarpu spolystachyus Rehd.leaves in the experimental hyperglycemic rats[J].Journal of Ethnopharmacology,2011,24(2):120-124.
[16]Wang Q W,Liu J J,Liu X,et al.Purification of phloridzin from Lithocarpus polystachyus Rehd.leaves using polymeric adsorbentsfunctionalized with glucosamine and β-cyclodextrin [J].ChemicalPapers,2014,11(7):32-35.
[17]王坤,李开祥,陈金艳,等.野生多穗柯主要活性成分及其含量变化[J].经济林研究,2016,34(4):96-100,122.
[18]段春芳,熊贤坤,任树琴,等.几种食用木薯品种生长发育及产量性状分析[J].西南农业学报,2017,30(12):2764-2768.
[19]董承光,王娟,周小凤,等.新疆陆地棉品种资源的主成分分析和聚类分析[J].西南农业学报,2016,29(8):1798-1805.
[20]许杰,王封义,班启堂,等.14个三年桐种源果实经济性状评价[J].种子,2017,36(9):97-100.
[21]雷伟侠,范志雄,阮怀明.甘蓝型油菜种质资源主要经济性状关系分析[J].湖北农业科学,2018,57(13):13-16.
[2]中国科学院植物研究所.中国高等植物图鉴(第1册) [M].北京:科学出版社,1972:434.
[3]何春年,彭勇,肖伟,等.多穗柯甜茶的研究进展[J].时珍国医国药,2012,23(5):1253-1255.
[4]Dudash J,Zhang X,Zeck R,et al.Glycosylated dihydrochalcones as potent and selective sodium glucose co-transporter 2 (SGLT-2) inhibitors[J].Bioorganic & Medicinal Chemistry Letters,2004,14(20):5121-5125.
[5]董华强,宁正祥,于立静,等.多穗柯黄酮根皮苷对糖尿病小鼠的降血糖血脂效果[J].食品科学,2006,27(12):714-718.
[6]Yang W M,Liu J K,Qin X D,et al.Antioxidant activities of three dihydrochalcone glucosides from leaves of Lithocarpus pachyphyllus[J].Zeitschrift für Naturforschung C,2004,59(7-8):481-484.
[7]燕妮,王佐,高雄,等.多穗柯挥发性组分分析及抗癌活性的初步研究[J].现代食品科技,2017,33(3):35-39,73.
[8]李胜华,伍贤进,曾军英,等.多穗柯中黄酮类成分研究[J].中草药,2010,41(21):1967-1969.
[9]肖坤福,廖晓峰.多穗柯黄酮类化合物的分离及鉴定[J].林产化学与工业,2006,26(3):85-87.
[10]董华强,宁正祥,崔志新,等.微波辅助提取多穗柯嫩叶黄酮工艺研究[J].农业工程学报,2007,23(2):213-217.
[11]李胜华,伍贤进,杨青丹,等.多穗柯化学成分研究[J].中药材,2010,33(4):549-551.
[12]Chen Z H,Zhang R J,Wu J,et al.New dihydrochalcone glycosides from Lithocarpus litseifolius and the phenomenon of C-H C-D exchange observed in NMR spectra of phenolic components[J].Journal of Asian Natural Products Research,2009,11(6):508-513.
[13]李胜华,伍贤进,向晓军.多穗柯有效成分的提取及抑菌效果研究[J].食品科技,2010,35(3):211-214.
[14]单思聪,孙爽.超临界萃取多穗柯黄酮及其抗运动氧化功能研究[J].食品研究与开发,2016,37(20):67-70.
[15]Hou S Z,Chen S X,Huang S,et al.The hypoglycemic activity of Lithocarpu spolystachyus Rehd.leaves in the experimental hyperglycemic rats[J].Journal of Ethnopharmacology,2011,24(2):120-124.
[16]Wang Q W,Liu J J,Liu X,et al.Purification of phloridzin from Lithocarpus polystachyus Rehd.leaves using polymeric adsorbentsfunctionalized with glucosamine and β-cyclodextrin [J].ChemicalPapers,2014,11(7):32-35.
[17]王坤,李开祥,陈金艳,等.野生多穗柯主要活性成分及其含量变化[J].经济林研究,2016,34(4):96-100,122.
[18]段春芳,熊贤坤,任树琴,等.几种食用木薯品种生长发育及产量性状分析[J].西南农业学报,2017,30(12):2764-2768.
[19]董承光,王娟,周小凤,等.新疆陆地棉品种资源的主成分分析和聚类分析[J].西南农业学报,2016,29(8):1798-1805.
[20]许杰,王封义,班启堂,等.14个三年桐种源果实经济性状评价[J].种子,2017,36(9):97-100.
[21]雷伟侠,范志雄,阮怀明.甘蓝型油菜种质资源主要经济性状关系分析[J].湖北农业科学,2018,57(13):13-16.