8种炮制方法对山茱萸脂溶性成分的影响
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摘要
目的考察8种炮制方法对山茱萸Cornus officinalis Sieb.et Zucc.脂溶性成分组成和含有量的影响。方法 8种方法分别炮制山萸肉,以乙醚为提取溶剂,采用索氏回流提取法得到山萸肉生品及其炮制品脂溶性成分,将脂溶性成分衍生化,GC-MS法进行定性鉴定和定量测定。结果 8种炮制方法均能提高山萸肉脂溶性成分提取率。与生品比较,各炮制品脂溶性成分数量除清炒、醋蒸和酒蒸外均降低,检出成分含有量均为100%,主要组成为游离脂肪酸;除麸炒品、醋蒸品和核外,各炮制品中不饱和脂肪酸含有量均低于生品,而饱和脂肪酸含有量高于生品。结论不同炮制方法对山萸肉脂溶性成分组成和含有量的影响有明显差异。
引文
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[14] Chung H K, Cho Y, Do H J, et al. Plasma phospholipid arachidonic acid and lignoceric acid are associated with the risk of cardioembolic stroke[J]. Nutr Res, 2015, 35(11): 1001-1008.
[15] Ni Y, Zhao L, Yu H, et al. Circulating unsaturated fatty acids delineate the metabolic status of obese individuals[J]. Ebio Medicine, 2015, 2(10): 1513-1522.
[16] Wang X, Gupta J, Kersalake M, et al. Trans-11 vaccenic acid improves insulin secretion in models of type 2 diabetes in vivo and in vitro[J]. Mol Nutr Food Res, 2016, 60(4): 846-857.
[17] Sassa T, Wakashima T, Ohno Y, et al. Lorenzo’s oil inhibits ELOVL1 and lowers the level of sphingomyelin with a saturated very long-chain fatty acid[J]. J Lipid Res, 2014, 55(3): 524-530.
[2] Huang J, Zhang Y, Dong L, et al. Ethnopharmacology, phytochemistry, and pharmacology of Cornus officinalis Sieb. et Zucc[J]. J Ethnopharmacol, 2018, 213: 280-301.
[3] 王孝涛. 历代中药炮制法汇典[M]. 南昌:江西科学技术出版社, 1998, 216-218.
[4] 冯冬兰. 山茱萸“合核”入药的本草考证及现代研究[J]. 中医学报, 2015, 30(2): 253-254.
[5] 刘艳丽, 晋海军, 何建华, 等. 山茱萸果肉、果核中甾醇和脂肪酸构成分析[J]. 时珍国医国药, 2012, 23(3): 633-634.
[6] 白成科, 李鹏毛, 王喆之. 山茱萸肉和种子脂溶性成分的GC-MS分析[J]. 食品科学, 2007, 28(11): 493-496.
[7] 许冬谨, 刘再强, 陈华师. 盐制山茱萸炮制工艺研究[J]. 中国实验方剂学杂志, 2011, 17(13): 46-47.
[8] 王同珍, 陈孝建, 安爱, 等. 气相色谱—质谱技术结合化学计量学对5种动物油进行判别分析[J]. 分析测试学报, 2016, 35(5): 557-562.
[9] 李红伟, 石延榜, 田连起, 等. 5种炮制方法对南葶苈子脂肪油组成和含量的影响[J]. 中成药, 2017, 39(8): 1661-1665.
[10] 吴慧, 单国顺, 赵文龙, 等. 不同麦麸对白术炮制品质量的影响[J]. 中国实验方剂学杂志, 2014, 20(6): 55-60.
[11] Lu H, Hao L, Li S, et al. Elevated circulating stearic acid leads to a major lipotoxic effect on mouse pancreatic beta cells in hyperlipidaemia via a miR-34a-5p-mediated PERK/p53-dependent pathway[J]. Diabetologia, 2016, 59(6): 1247-1257.
[12] Park E J, Lee A Y, Park S, et al. Multiple pathways are involved in palmitic acid-induced toxicity[J]. Food Chem Toxicol, 2014, 67: 26-34.
[13] Martinez L, Torres S, Baulies A, et al. Myristic acid potentiates palmitic acid-induced lipotoxicity and steatohepatitis associated with lipodystrophy by sustaining de novo ceramide synthesis[J]. Oncotarget, 2015, 6(39): 41479-41496.
[14] Chung H K, Cho Y, Do H J, et al. Plasma phospholipid arachidonic acid and lignoceric acid are associated with the risk of cardioembolic stroke[J]. Nutr Res, 2015, 35(11): 1001-1008.
[15] Ni Y, Zhao L, Yu H, et al. Circulating unsaturated fatty acids delineate the metabolic status of obese individuals[J]. Ebio Medicine, 2015, 2(10): 1513-1522.
[16] Wang X, Gupta J, Kersalake M, et al. Trans-11 vaccenic acid improves insulin secretion in models of type 2 diabetes in vivo and in vitro[J]. Mol Nutr Food Res, 2016, 60(4): 846-857.
[17] Sassa T, Wakashima T, Ohno Y, et al. Lorenzo’s oil inhibits ELOVL1 and lowers the level of sphingomyelin with a saturated very long-chain fatty acid[J]. J Lipid Res, 2014, 55(3): 524-530.