实验动物病原PCR检测方法系列团体标准的编制
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摘要
实验动物质量检测是实验动物质量管理的重要手段,标准的制定和更新是促进我国实验动物质量提升的主要方法。近年来,实验动物新的疾病病原不断出现,新的检测技术不断更新,仅靠国家标准的制定和更新已不能满足我国实验动物的质量管理的需求。利用团体标准搞创新的优势,结合实际需求,制定了一批实验动物病原PCR检测方法团体标准。这些团体标准是国家标准的有力补充,对促进我国实验动物质量提升具有重要作用。
Determination of the quality of laboratory animals is essential for managing them, and the formulation and revision of standards are the main approaches for promoting the quality of laboratory animals in our contry. In recent years, new pathogens of laboratory animals have emerged and novel detection techniques are constantly being updated. The establishment and renewal of national standards do not meet the quality management requirements of laboratory animals in our country. In this project, taking advantage of social organization standards for innovation, a number of laboratory animal social organization standards on PCR for pathogen detection were developed, combined with the practical needs of laboratory animals. These standards are useful supplements to national standards and should play an important role in improving the quality of laboratory animals in our country.
Determination of the quality of laboratory animals is essential for managing them, and the formulation and revision of standards are the main approaches for promoting the quality of laboratory animals in our contry. In recent years, new pathogens of laboratory animals have emerged and novel detection techniques are constantly being updated. The establishment and renewal of national standards do not meet the quality management requirements of laboratory animals in our country. In this project, taking advantage of social organization standards for innovation, a number of laboratory animal social organization standards on PCR for pathogen detection were developed, combined with the practical needs of laboratory animals. These standards are useful supplements to national standards and should play an important role in improving the quality of laboratory animals in our country.
引文
[1] 贺争鸣, 李根平, 赵德明, 等.把握改革机遇,建立严谨的实验动物技术标准体系[J].实验动物与比较医学,2016, 36(1):57-60.
[2] 贺争鸣, 李根平, 陈梅丽, 等. 实验动物地方标准的查新和确认机制的建立及地方标准的应用[J].实验动物科学, 2017, 34(2):4-50.
[3] Mahler M, Berard M, Feinstein R, et al. FELASA recommendations for the health monitoring of mouse, rat, hamster, guinea pig and rabbit colonies in breeding and experimental units[J]. Lab Anim, 2014, 48(3):178-192.
[4] Feng S, Ku K, Hodzic E, et al. Differential detection of five mouse-infecting helicobacter species by multiplex PCR[J]. Clin Diagn Lab Immunol, 2005,(12):531-536.
[5] Wan CH, Bauer BA, Pintel DJ, et al.Detection of rat parvovirus type 1 and rat minute virus type 1 by polymerase chain reaction[J]. Lab Anim, 2006, 40(1):63-9.
[6] Yuan W, Wang J, Xu FJ, et al. Development of a duplex real-time RT-PCR for the simultaneous detection and differentiation of Theiler’s murine Encephalomyelitis virus and rat theilovirus[J].Virol Methods, 2016,236,139-146.
[7] Bootz F, Sieber I, Popovic D, et al. Comparison of the sensitivity of in vivo antibody production tests with in vitro PCR-based methods to detect infectious contamination of biological materials[J]. Lab Anim, 2003 37: 341-351.
[8] Besselsen DG,Wagner AM, Loganbill JK. Detection of rodent coronaviruses by use of fluorogenic reverse transcriptase-polymerase chain reaction analysis[J]. Comp Med, 2002,52(2):111-116.
[9] Redig AJ, Besselsen DG. Detection of rodent parvoviruses by use of fluorogenic nuclease polymerase chain reaction assays[J].Comp Med, 2001, 51(4):326-31.
[10] 袁文,张钰,王静,等.小鼠脑脊髓炎病毒非编码蛋白(UTR)片段实时荧光定量PCR标准品的构建[J]. 实验动物与比较医学, 2012,32(1):1-6.
[11] 袁文,王静,黄韧, 等. 结合内标的小鼠诺如病毒荧光定量RT-PCR检测方法的建立及应用[J].中国实验动物学报,2015,23(1):49-56.
[12] 尹雪琴,袁文,王静,等.实时荧光定量TaqMan PCR检测小鼠多瘤病毒方法的建立[J]. 中国比较医学杂志, 2015, 25(6):53-58.
[13] White JA, Todd PA, Rosenthal AN,et al. Development of a generic real-time PCR assay for simultaneous detection of proviral DNA of simian Betaretrovirus serotypes 1, 2, 3, 4 and 5 and secondary uniplex assays for specific serotype identification[J]. J Vriol Methods,2009,162(1-2):148-154.
[14] 王静, 张鈺, 闵凡贵, 等.猴免疫缺陷病毒(SIV) 实时荧光定量 PCR检测方法的建立 [J].中国比较医学杂志, 2013, 23(9): 68-72.
[15] Pritchett-Corning KR, Cosentino J, Clifford CB. Contemporary prevalence of infectious agents in laboratory mice and rats[J]. Lab Anim. 2009;43(2):165-73.
[16] 袁文,张钰,刘忠华,等. 广东省实验小鼠自然感染鼠诺如病毒的调查[J]. 中国比较医学杂志, 2009,20(2):78-82.
[17] 袁文,张钰,黄碧洪,等.小鼠脑脊髓炎病毒自然感染调查以及人工感染小鼠试验[J].中国比较医学杂志,2017,27 (4):75-81.
[18] 王翠娥,陈立超,周倩,等.实验大鼠和小鼠多种病毒的血清学检测结果分析[J]. 实验动物科学, 2014,31(2):20-24.
[19] 潘金春,赵维波,陈梅玲,等.2013年～2015年广东地区实验小鼠和大鼠微生物学及寄生虫学调查[J].中国比较医学杂志,2017,27 (2):64-69.
[2] 贺争鸣, 李根平, 陈梅丽, 等. 实验动物地方标准的查新和确认机制的建立及地方标准的应用[J].实验动物科学, 2017, 34(2):4-50.
[3] Mahler M, Berard M, Feinstein R, et al. FELASA recommendations for the health monitoring of mouse, rat, hamster, guinea pig and rabbit colonies in breeding and experimental units[J]. Lab Anim, 2014, 48(3):178-192.
[4] Feng S, Ku K, Hodzic E, et al. Differential detection of five mouse-infecting helicobacter species by multiplex PCR[J]. Clin Diagn Lab Immunol, 2005,(12):531-536.
[5] Wan CH, Bauer BA, Pintel DJ, et al.Detection of rat parvovirus type 1 and rat minute virus type 1 by polymerase chain reaction[J]. Lab Anim, 2006, 40(1):63-9.
[6] Yuan W, Wang J, Xu FJ, et al. Development of a duplex real-time RT-PCR for the simultaneous detection and differentiation of Theiler’s murine Encephalomyelitis virus and rat theilovirus[J].Virol Methods, 2016,236,139-146.
[7] Bootz F, Sieber I, Popovic D, et al. Comparison of the sensitivity of in vivo antibody production tests with in vitro PCR-based methods to detect infectious contamination of biological materials[J]. Lab Anim, 2003 37: 341-351.
[8] Besselsen DG,Wagner AM, Loganbill JK. Detection of rodent coronaviruses by use of fluorogenic reverse transcriptase-polymerase chain reaction analysis[J]. Comp Med, 2002,52(2):111-116.
[9] Redig AJ, Besselsen DG. Detection of rodent parvoviruses by use of fluorogenic nuclease polymerase chain reaction assays[J].Comp Med, 2001, 51(4):326-31.
[10] 袁文,张钰,王静,等.小鼠脑脊髓炎病毒非编码蛋白(UTR)片段实时荧光定量PCR标准品的构建[J]. 实验动物与比较医学, 2012,32(1):1-6.
[11] 袁文,王静,黄韧, 等. 结合内标的小鼠诺如病毒荧光定量RT-PCR检测方法的建立及应用[J].中国实验动物学报,2015,23(1):49-56.
[12] 尹雪琴,袁文,王静,等.实时荧光定量TaqMan PCR检测小鼠多瘤病毒方法的建立[J]. 中国比较医学杂志, 2015, 25(6):53-58.
[13] White JA, Todd PA, Rosenthal AN,et al. Development of a generic real-time PCR assay for simultaneous detection of proviral DNA of simian Betaretrovirus serotypes 1, 2, 3, 4 and 5 and secondary uniplex assays for specific serotype identification[J]. J Vriol Methods,2009,162(1-2):148-154.
[14] 王静, 张鈺, 闵凡贵, 等.猴免疫缺陷病毒(SIV) 实时荧光定量 PCR检测方法的建立 [J].中国比较医学杂志, 2013, 23(9): 68-72.
[15] Pritchett-Corning KR, Cosentino J, Clifford CB. Contemporary prevalence of infectious agents in laboratory mice and rats[J]. Lab Anim. 2009;43(2):165-73.
[16] 袁文,张钰,刘忠华,等. 广东省实验小鼠自然感染鼠诺如病毒的调查[J]. 中国比较医学杂志, 2009,20(2):78-82.
[17] 袁文,张钰,黄碧洪,等.小鼠脑脊髓炎病毒自然感染调查以及人工感染小鼠试验[J].中国比较医学杂志,2017,27 (4):75-81.
[18] 王翠娥,陈立超,周倩,等.实验大鼠和小鼠多种病毒的血清学检测结果分析[J]. 实验动物科学, 2014,31(2):20-24.
[19] 潘金春,赵维波,陈梅玲,等.2013年～2015年广东地区实验小鼠和大鼠微生物学及寄生虫学调查[J].中国比较医学杂志,2017,27 (2):64-69.
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