膀胱肿瘤患者免疫细胞及其细胞因子检测的研究
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摘要
探讨膀胱肿瘤患者多种免疫细胞及其细胞因子与临床病理指标的相关性及意义。采用流式细胞数检测40例膀胱癌患者和15例正常健康体检者的外周血中T细胞及其亚群、B细胞及NK细胞的表达。用ELISA方法检测所有人的血清和尿中IL-10、IL-35、TGF-β1含量。结果显示:膀胱肿瘤患者外周血总T细胞、NK细胞及CD+4T、CD+4/CD+8比值均显著低于正常组(P<0.05);膀胱肿瘤患者外周血CD+8T细胞和Treg细胞均显著高于正常组(P<0.05);膀胱肿瘤患者血清和尿中IL-10、IL-35、TGF-β1含量极显著高于正常组(P<0.01)。膀胱肿瘤患者抗肿瘤免疫综合功能下降,血清和尿中IL-10、IL-35、TGF-β1水平检测对膀胱肿瘤的诊断、分期、预后判定有重要意义。
To discuss the correlation and meanings between the bladder tumor patients'immunocyte &cytokines and clinicopathological markers.The flow cytometry is used to detect the expression of T cell &its subgroup,B cells and NK cells in the peripheral blood of the 40 bladder cancer patients and 15 normal and healthy persons in physical examination.The ELISA method is used to detect the IL-10,IL-35 and TGF-β1contents in the serum and urine of all persons.The results show that,The bladder tumor patients'total ratio of peripheral blood T cell,NK cell,CD+4T and CD+4/CD+8is significantly lower than that of the normal group(P<0.05).The bladder tumor patients'peripheral blood CD+8T cell and Treg cell are significantly higher than these of the normal group(P<0.05).The content of IL-10,IL-35 and TGF-β1in the serum and urine of the bladder tumor patients is significantly higher than the normal group(P<0.01).The antitumor immunity comprehensive function of the bladder tumor patients is lowered,the detection of the IL-10,IL-35 and TGF-β1level in the serum and urine has significant meaning to the diagnosis,analysis and prognosis determination of the bladder tumor.
To discuss the correlation and meanings between the bladder tumor patients'immunocyte &cytokines and clinicopathological markers.The flow cytometry is used to detect the expression of T cell &its subgroup,B cells and NK cells in the peripheral blood of the 40 bladder cancer patients and 15 normal and healthy persons in physical examination.The ELISA method is used to detect the IL-10,IL-35 and TGF-β1contents in the serum and urine of all persons.The results show that,The bladder tumor patients'total ratio of peripheral blood T cell,NK cell,CD+4T and CD+4/CD+8is significantly lower than that of the normal group(P<0.05).The bladder tumor patients'peripheral blood CD+8T cell and Treg cell are significantly higher than these of the normal group(P<0.05).The content of IL-10,IL-35 and TGF-β1in the serum and urine of the bladder tumor patients is significantly higher than the normal group(P<0.01).The antitumor immunity comprehensive function of the bladder tumor patients is lowered,the detection of the IL-10,IL-35 and TGF-β1level in the serum and urine has significant meaning to the diagnosis,analysis and prognosis determination of the bladder tumor.
引文
[1]郝捷,陈万青.膀胱:2012年中国肿瘤登记年报[M].北京:军事医学科学出版社,2012.
[2]Richards K A.Use of Some Antiplatelet Agents Appears Safe in Patients Undergoing Transurethral Resection of Bladder Tumor[J].Transl Androl Urol,2018,7(Suppl 2):236-237.
[3]Kerkar S P,Restifo N P.Cellular Constituents of Immune Escape with in the Tumor Microenvironment[J].Cancer Res,2012,72(13):3125-3130.
[4]Palucka K,Ueno H,Fay J,et al.Dendritic Cells and Immunity Against Cancer[J].J Intern Med,2011,269(1):64-73.
[5]Dunn G P,Bruce A T,Ikeda H,et al.Cancer Immunoediting:From Immunosurveillance to Tumor Escape[J].Nat Immunol,2002,3:991-998.
[6]方丽,陈乔尔.Fas L的反义基因在肿瘤治疗中的作用[J].安徽医药,2013,17(3):370-372.
[7]Chiarella P,Bruzzo J,Meiss R P,et al.Concomitant Tumor Resistance[J].Cancer Lett,2012,324(2):133-141.
[8]Whiteside T L,Schuler P,Schilling B.Induced and Natural Regulatory T Cells in Human Cancer[J].Expert Opin Biol T-her,2012,12(10):1383-1397.
[9]Bergmann C,Strauss L,Wang Y,et al.T Regulatory Type 1Cells in Squamous Cell Carcinoma of the Head and Neck:Mechanisms of Suppression and Expansion in Advanced Disease[J].Clin Cancer Res,2008,14(12):3706-3715.
[10]David M Gravano,Dario A.The Battle Against Immunopathology:Infectious Tolerance Mediated by Regulatory T Cells[J].Cell Mol Life Sci.,2012,69(12):1997-2008.
[11]Kim S,Buchlis G,Fridlender Z G,et al.Systemic Blockade of Trans-forming Growth Factor-beta Signaling Augments the Efficacy of Immunogene Therapy[J].Cancer Res,2008,68(24):10247-10256.
[12]Brian M Olson,Jeremy A.Interleukin 35:A Key Mediator of Suppression and the Propagation of Infectious Tolerance[J].Frontiers in Immunology,2013,4:1-12.
[13]洪超群,陈炯玉,杨卫平,等.食管鳞癌患者手术前后外周血T细胞亚群与NK、NKT细胞检测的临床意义[J].现代肿瘤医学,2010,18(6):1113-1115.
[2]Richards K A.Use of Some Antiplatelet Agents Appears Safe in Patients Undergoing Transurethral Resection of Bladder Tumor[J].Transl Androl Urol,2018,7(Suppl 2):236-237.
[3]Kerkar S P,Restifo N P.Cellular Constituents of Immune Escape with in the Tumor Microenvironment[J].Cancer Res,2012,72(13):3125-3130.
[4]Palucka K,Ueno H,Fay J,et al.Dendritic Cells and Immunity Against Cancer[J].J Intern Med,2011,269(1):64-73.
[5]Dunn G P,Bruce A T,Ikeda H,et al.Cancer Immunoediting:From Immunosurveillance to Tumor Escape[J].Nat Immunol,2002,3:991-998.
[6]方丽,陈乔尔.Fas L的反义基因在肿瘤治疗中的作用[J].安徽医药,2013,17(3):370-372.
[7]Chiarella P,Bruzzo J,Meiss R P,et al.Concomitant Tumor Resistance[J].Cancer Lett,2012,324(2):133-141.
[8]Whiteside T L,Schuler P,Schilling B.Induced and Natural Regulatory T Cells in Human Cancer[J].Expert Opin Biol T-her,2012,12(10):1383-1397.
[9]Bergmann C,Strauss L,Wang Y,et al.T Regulatory Type 1Cells in Squamous Cell Carcinoma of the Head and Neck:Mechanisms of Suppression and Expansion in Advanced Disease[J].Clin Cancer Res,2008,14(12):3706-3715.
[10]David M Gravano,Dario A.The Battle Against Immunopathology:Infectious Tolerance Mediated by Regulatory T Cells[J].Cell Mol Life Sci.,2012,69(12):1997-2008.
[11]Kim S,Buchlis G,Fridlender Z G,et al.Systemic Blockade of Trans-forming Growth Factor-beta Signaling Augments the Efficacy of Immunogene Therapy[J].Cancer Res,2008,68(24):10247-10256.
[12]Brian M Olson,Jeremy A.Interleukin 35:A Key Mediator of Suppression and the Propagation of Infectious Tolerance[J].Frontiers in Immunology,2013,4:1-12.
[13]洪超群,陈炯玉,杨卫平,等.食管鳞癌患者手术前后外周血T细胞亚群与NK、NKT细胞检测的临床意义[J].现代肿瘤医学,2010,18(6):1113-1115.