伴生小麦对黄瓜抗坏血酸-谷胱甘肽循环及产量的影响
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摘要
以黄瓜品种"津早1号"为试材,采用田间试验的方法,研究了伴生小麦对黄瓜叶片抗坏血酸-谷胱甘肽(AsA-GSH)循环相关生理指标和产量的影响,以期为小麦与黄瓜伴生栽培模式的应用提供参考依据。结果表明:当第4片叶叶龄为50、60、70 d时,伴生小麦处理的黄瓜叶片丙二醛(MDA)含量均低于单作处理,而伴生处理的还原型谷胱甘肽(GSH)含量、GSH/氧化型谷胱甘肽(GSSG)均高于单作处理。另外,伴生处理各取样时期的抗坏血酸过氧化物酶(APX)、单脱氢抗坏血酸还原酶(MDHAR)、脱氢抗坏血酸还原酶(DHAR)和谷胱甘肽还原酶(GR)活性都高于单作处理,而伴生处理抗坏血酸(AsA)的含量仅在40、60、70 d时显著高于单作处理。总之,伴生小麦栽培模式在一定程度上增强了黄瓜叶片的抗坏血酸-谷胱甘肽循环,进而提高了黄瓜抗氧化能力。
Filed experiment was conducted to study the effects of companion cropping with wheat on ascorbate-glutathione cycle and yield of cucumber,which could provide theoretical basis for the application of wheat as companion crop with cucumber.The results showed that the MDA content of cucumber fourth leaf was lower in the treatment of companion cropping with wheat than that in monoculture at 50,60 days and 70 days.However,a reverse trend was observed in the indicators of GSH and GSH/GSSG in the treatment of companion cropping with wheat at the same sampling time.Moreover,companion cropping with wheat promoted the activities of APX,MDHAR,DHAR and GR of cucumber leaf at all sampling time relative to monoculture.In addition,the content of AsA in the treatment of companion cropping with wheat was significantly increased at 40,60 days and 70 days compared with the monoculture.In conclusion,the model of companion cropping with wheat could enhance the ascorbate-glutathione cycle in cucumber leaves to some extent,and thus improve the abilities of defensing oxidation in cucumber.
Filed experiment was conducted to study the effects of companion cropping with wheat on ascorbate-glutathione cycle and yield of cucumber,which could provide theoretical basis for the application of wheat as companion crop with cucumber.The results showed that the MDA content of cucumber fourth leaf was lower in the treatment of companion cropping with wheat than that in monoculture at 50,60 days and 70 days.However,a reverse trend was observed in the indicators of GSH and GSH/GSSG in the treatment of companion cropping with wheat at the same sampling time.Moreover,companion cropping with wheat promoted the activities of APX,MDHAR,DHAR and GR of cucumber leaf at all sampling time relative to monoculture.In addition,the content of AsA in the treatment of companion cropping with wheat was significantly increased at 40,60 days and 70 days compared with the monoculture.In conclusion,the model of companion cropping with wheat could enhance the ascorbate-glutathione cycle in cucumber leaves to some extent,and thus improve the abilities of defensing oxidation in cucumber.
引文
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[23] 郭伟,薛盈文,于崧,等.邻苯二甲酸与对羟基苯甲酸对燕麦幼苗生长及抗氧化特性的影响[J].植物生理学报,2017(10):1885-1892.
[24] 马亚飞,杨平,吴凤芝.不同品系小麦根系分泌物对黄瓜化感作用的初步研究[J].中国蔬菜,2011,1(10):23-27.
[25] 杨平.小麦根系浸提液对黄瓜幼苗化感作用的生理生化机制[D].哈尔滨:东北农业大学,2011.
[26] 吴凤芝,李敏,曹鹏,等.小麦根系分泌物对黄瓜生长及土壤真菌群落结构的影响[J].应用生态学报,2014,25(10):2861-2867.
[27] 高丹美.填闲小麦对黄瓜幼苗的化感作用和营养资源利用的影响[D].哈尔滨:东北农业大学,2015.
[28] 邢燕菊,韩金龙,徐立华,等.禾本科专一性根系分泌物:麦根酸类物质研究进展[J].山东农业科学,2010(10):62-65.
[29] KANAI M,MIWA M,KUCHINO Y,et al.Presence of branched portion in poly(adenosine diphosphate ribose) in vivo[J].Journal of Biological Chemistry,1982,257(11):6217-6223.
[2] 宫再英,周新刚,潘凯,等.不同比例的玉米、小麦、菊芋秸秆对黄瓜幼苗生长及相关生理指标的影响[J].作物杂志,2015(6):126-131.
[3] SAINJU U M,SINGH B P,WHITEHEAD W F.Comparison of the effects of cover crops and nitrogen fertilization on tomato yield,root growth,and soil properties[J].Scientia Horticulturae,2001,91(3):201-214.
[4] MARIA D,EWA S P,ZBIGNIEW K.The redox state and activity of superoxide dismutase classes in Arabidopsis thaliana under cadmium or copper stress[J].Chemosphere,2007,67(1):188-193.
[5] 孙德祥,秦海霞,丁会纳,等.硅肥对干旱胁迫下冬小麦旗叶抗坏血酸-谷胱甘肽循环系统及产量的影响[J].麦类作物学报,2015,35(5):693-698.
[6] 高春琦,吴凤芝.伴生小麦对黄瓜生长及生理指标的影响[J].中国蔬菜,2014,1(10):24.
[7] 李圆圆,张婧,韩庆姝,等.伴生小麦对黄瓜叶片衰老期间糖代谢相关指标的影响[J].中国蔬菜,2018(1):39-44.
[8] 王学奎,黄见良.植物生理生化实验原理与技术[M].北京:高等教育出版社,2015.
[9] JIMéNEZ A,HERNáNDEZ J A,RíO L A D,et al.Evidence for the presence of the ascorbate-glutathione cycle in mitochondria and peroxisomes of pea leaves[J].Plant Physiology,1997,114(1):275-284.
[10] LEI Y,YIN C,LI C.Differences in some morphological,physiological,and biochemical responses to drought stress in two contrasting populations of Populus przewalskii[J].Physiologia Plantarum,2006,127(2):182-191.
[11] PINTO M C D E,FRANCIS D,GARA L D E.The redox state of the ascorbate-dehydroascorbate pair as a specific sensor of cell division in tobacco BY-2 cells[J].Protoplasma,1999,209(1-2):90-97.
[12] 王学奎.植物生理生化实验原理和技术[M].2版.北京:高等教育出版社,2006.
[13] 李忠光,杜朝昆,龚明.在单一提取系统中同时测定植物AsA/DHA和GSH/GSSG[J].云南师范大学学报(自然科学版),2003,23(3):67-70.
[14] MUDALKAR S,SREEHARSHA R V,REDDY A R.Involvement of glyoxalases and glutathione reductase in conferring abiotic stress tolerance to Jatropha curcas L.[J].Environmental and Experimental Botany,Elsevier BV,2017,134:141-150.
[15] 董守坤,马玉玲,李爽,等.干旱胁迫及复水对大豆抗坏血酸-谷胱甘肽循环的影响[J].东北农业大学学报,2018(1):10-18.
[16] 张福建,陈昱,杨有新,等.伴生大麦和芥菜对连作辣椒叶片保护酶活性及根际土壤环境的影响[J].中国蔬菜,2018(2):42-46.
[17] 陈昱,张福建,杨有新,等.伴生芹菜和紫背天葵对连作豇豆生长发育及根际土壤环境的影响[J].2018,24(5):1406-1414.
[18] 蒋芝华,李鹏程,孙骏威,等.入侵植物刺苋对水稻化感效应及抗氧化系统的影响[J].中国计量学院学报,2017,28(1):68-75.
[19] 靳雯棋,毕英飞,王晶,等.野山参与园参抗坏血酸-谷胱甘肽循环代谢差异的比较[J].中草药,2017,48(2):373-376.
[20] 曹国璠,丁飞.间作栽培措施对辣椒主要品质指标的影响[J].南方农业学报,2011,42(9):1112-1115.
[21] NOCTOR G,MHAMDI A,CHAOUCH S,et al.Glutathione in plants:An integrated overview[J].Plant,Cell and Environment,2012,35(2):454-484.
[22] 江力,汤一卒,张荣铣,等.肥料吸收活化剂对烟叶抗坏血酸-谷胱甘肽循环系统及钾含量的影响[J].南京农业大学学报,2000,23(4):13-16.
[23] 郭伟,薛盈文,于崧,等.邻苯二甲酸与对羟基苯甲酸对燕麦幼苗生长及抗氧化特性的影响[J].植物生理学报,2017(10):1885-1892.
[24] 马亚飞,杨平,吴凤芝.不同品系小麦根系分泌物对黄瓜化感作用的初步研究[J].中国蔬菜,2011,1(10):23-27.
[25] 杨平.小麦根系浸提液对黄瓜幼苗化感作用的生理生化机制[D].哈尔滨:东北农业大学,2011.
[26] 吴凤芝,李敏,曹鹏,等.小麦根系分泌物对黄瓜生长及土壤真菌群落结构的影响[J].应用生态学报,2014,25(10):2861-2867.
[27] 高丹美.填闲小麦对黄瓜幼苗的化感作用和营养资源利用的影响[D].哈尔滨:东北农业大学,2015.
[28] 邢燕菊,韩金龙,徐立华,等.禾本科专一性根系分泌物:麦根酸类物质研究进展[J].山东农业科学,2010(10):62-65.
[29] KANAI M,MIWA M,KUCHINO Y,et al.Presence of branched portion in poly(adenosine diphosphate ribose) in vivo[J].Journal of Biological Chemistry,1982,257(11):6217-6223.