赤霉素与多效唑对甘蔗愈伤再生苗植株内源激素含量的影响
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摘要
在甘蔗品种‘新台糖22号’(‘ROC22’)愈伤再生苗生长前期,分别使用赤霉素(GA)与多效唑(PP333)均匀喷施于植株叶片等生长部位,对植株生长素(IAA)、赤霉素(GA)、脱落酸(ABA)、玉米素核苷(ZR) 4种内源激素含量变化进行分析。结果表明:(1) GA处理甘蔗植株叶片中IAA含量较对照变化较大; GA含量的变化趋势与对照相似; ABA含量逐渐上升; ZR在前期稍高于对照,后期与对照一样。PP333处理甘蔗叶片中IAA、 GA、 ABA与ZR含量初期较高,之后比对照稍低。GA与PP333配合处理后,甘蔗叶片中的IAA、 ABA含量与对照有相似的变化趋势; GA含量较对照变化较大; ZR含量有所上升。(2) GA处理后, GA/IAA、 ABA/IAA、 ZR/IAA比值变化趋势相似。PP333处理的GA/IAA比值变化趋势比较平稳, ABA/IAA、 ZR/IAA比值则比较活跃。GA与PP333配合处理的甘蔗叶片GA/IAA比值前期稍有下降而后上升; ABA/IAA、 ZR/IAA比值变化趋势较一致。经GA与PP333处理的甘蔗植株体内的内源激素IAA、 ZR含量及其GA/IAA、 ZR/IAA比值变化较大,激素之间的平衡调节着甘蔗茎生长过程。
Different concentrations of GA or PP_(333) were sprayed onto the leaves of sugarcane(Saccharum officinarum L.) plants regenerated from calli at an early stage.The changes of endogenous hormone(IAA,GA,ABA,and ZR) contents in the leaves of the regenerated sugarcane plants were detected and analyzed.The results showed that the leaf content of IAA had a higher change in the GA treatment than the control,and that the leaf GA content in the GA treatment had a similar change to that in the control.The leaf content of ABA increased gradually,and the leaf content of ZR was higher in the GA treatment at the early stage and then similar to that in the control.For the PP_(333) treatment the contents of IAA,GA,ABA and ZR were higher at the early stage,and then lower than those of the control.In the treatment with combined application of GA and PP_(333),the leaf contents of IAA and ABA were the same as those of the control,the leaf contents of GA changed markedly as against the control,and the leaf contents of ZR increased.The ratios of GA/IAA,ABA/IAA,and ZR/IAA had similar change after the leaves were treated with GA.In the PP_(333) treatment,the GA/IAA ratio had a steady change while the ratios of ABA/IAA and ZR/IAA had an active change.In the combined treatment with GA and PP_(333),the ratio of GA/IAA firstly decreased slightly and then increased,and the ratios of ABA/IAA and ZR/IAA had a similar change to those of the control.The contents of endogenous hormones IAA and ZR and the ratios of GA/IAA and ZR/IAA seemed to have a great change in the growth of the shoots of the sugarcane plants.
Different concentrations of GA or PP_(333) were sprayed onto the leaves of sugarcane(Saccharum officinarum L.) plants regenerated from calli at an early stage.The changes of endogenous hormone(IAA,GA,ABA,and ZR) contents in the leaves of the regenerated sugarcane plants were detected and analyzed.The results showed that the leaf content of IAA had a higher change in the GA treatment than the control,and that the leaf GA content in the GA treatment had a similar change to that in the control.The leaf content of ABA increased gradually,and the leaf content of ZR was higher in the GA treatment at the early stage and then similar to that in the control.For the PP_(333) treatment the contents of IAA,GA,ABA and ZR were higher at the early stage,and then lower than those of the control.In the treatment with combined application of GA and PP_(333),the leaf contents of IAA and ABA were the same as those of the control,the leaf contents of GA changed markedly as against the control,and the leaf contents of ZR increased.The ratios of GA/IAA,ABA/IAA,and ZR/IAA had similar change after the leaves were treated with GA.In the PP_(333) treatment,the GA/IAA ratio had a steady change while the ratios of ABA/IAA and ZR/IAA had an active change.In the combined treatment with GA and PP_(333),the ratio of GA/IAA firstly decreased slightly and then increased,and the ratios of ABA/IAA and ZR/IAA had a similar change to those of the control.The contents of endogenous hormones IAA and ZR and the ratios of GA/IAA and ZR/IAA seemed to have a great change in the growth of the shoots of the sugarcane plants.
引文
[1]李杨瑞.现代甘蔗学[M].北京:中国农业出版社, 2010.
[2] Sun T P, Gubler F. Molecular mechanism of gibberellin signaling in plants[J]. Annual Review of Plant Biology, 2004, 55:197-223.
[3]谭显平,吕达,李红梅,等.不同浓度赤霉素对果蔗产量和品质的影响研究初报[J].广西蔗糖, 2007,49(4):8-13, 27.
[4] Lu J J, Lei C Y, Zha L S, et al. A study on the application and residues of plant growth regulators in the fruit sugarcane grown in the sub-suitable region[J]. Journal of Agricultural Science, 2010,2(4):254-256.
[5] El-Lattief E A A, Bekheet M A. Quantitative and qualitative attributes of three sugarcane varieties as influenced by foliar spray of some growth regulators under upper Egypt conditions[J]. Sugar Tech, 2012, 14(4):345-350.
[6]吴建明,李杨瑞,王爱勤,等.赤霉素处理对甘蔗节间伸长及产质量的影响[J].中国糖业, 2010(4):24-26.
[7]吴建明,李杨瑞,王爱勤,等.赤霉素诱导甘蔗节间伸长的效应与相关酶活性的关系[J].西北植物学报,2010, 30(5):962-967.
[8]吴建明,李杨瑞,王爱勤,等.赤霉素诱导甘蔗节间伸长与内源激素变化的关系[J].热带作物学报,2009, 30(10):1 452-1 457.
[9]张国平, Chuula Argent.赤霉素与矮壮素或多效唑配合使用对小麦生育和代谢的影响[J].浙江农业学报,1992, 4(1):15-19.
[10]陈晓光,王振林,彭佃亮,等.种植密度与喷施多效唑对冬小麦抗倒伏能力和产量的影响[J].应用生态学报, 2011, 22(6):1 465-1 470.
[11]禤维言,张涛,黄永禄,等.喷施多效唑对甜高粱生长及生理特性的影响[J].作物杂志, 2011(5):73-76.
[12]班德宇,陈小英,黄诚梅.赤霉素与多效唑对甘蔗愈伤再生苗生长的影响[J].中国糖料, 2015, 37(5):20-23.
[13]游建华,何为中,曾慧,等.谈脱毒健康种苗在广西甘蔗生产的应用及效益展望[J].甘蔗糖业, 2001(1):13-17.
[14] Lee T S G. Multiplication of sugarcane by apex culture[J]. Turrialba, 1986, 36:231-235.
[15] Lee T S G. Micropropagation of sugarcane(Saccharum spp.)[J]. Plant Cell, Tissue and Organ Culture, 1987, 10:47-55.
[16] Garcia R, Cidade D, Castellar A, et al. In vitro morphogenesis patterns from shoot apices of sugarcane are determined by light and type of growth regulator[J]. Plant Cell, Tissue and Organ Culture, 2007, 90(2):181-190.
[17]李松,余坤兴,刘丽敏,等.甘蔗茎尖胚状体脱毒苗快繁技术研究[J].中国糖料, 2010(4):1-5, 8.
[18]李松,余坤兴,刘丽敏,等.甘蔗茎尖胚状体脱毒苗快繁技术研究[J].江苏农业科学, 2011, 39(2):83-87.
[19]何钟佩.农作物化学控制实验指导[M].北京:北京农业大学出版社, 1993:60-68.
[20] Pimenta Lange M J, Lange T. Gibberellin biosynthesis and the regulation of plant de velopment[J]. Plant Biology(Stuttg), 2006, 8(3):281-290.
[21]尚玉磊,李春喜,邵云,等.禾本科主要作物生育初期内源激素动态及其作用的比较[J].华北农学报,2004, 19(4):47-50.
[22] Hoffmann-Benning S, Kende H. On the role of abscisic acid and gibberellin in the regulation of growth in rice[J]. Plant Physiology, 1992, 99(3):1 156-1 161.
[23]叶梅荣,朱昌华,甘立军,等.激素间相互作用对植物茎伸长生长的调控综述[J].中国农学通报,2007, 23(4):228-231.
[24]房增国,赵秀芬,高祖明.多效唑对苗期稻麦叶片内源游离态激素含量的影响[J].莱阳农学院学报,2005, 22(2):101-104.
[25]陈俊伟,谢鸣,秦巧平.植物糖信号与激素信号之间的联系[J].植物生理学通讯, 2005, 41(3):279-285.
[26]朱云集,李向阳,郭天财,等.不同冠温特征冬小麦籽粒灌浆过程中内源激素含量的变化[J].植物生理学通讯, 2005, 41(6):720-724.
[27]王忠,王三根,李合生.植物生理学[M].北京:中国农业出版社, 2000:264-311.
[28]樊金娟,李雪梅,阮燕晔,等.杂交水稻及其亲本灌浆过程中内源激素含量的变化[J].植物生理学通讯,2004 40(2):146-148.
[29]李春俭.乙烯在豌豆中植株顶端优势中的作用[J].植物生理学报, 1997, 23(3):283-287.
[2] Sun T P, Gubler F. Molecular mechanism of gibberellin signaling in plants[J]. Annual Review of Plant Biology, 2004, 55:197-223.
[3]谭显平,吕达,李红梅,等.不同浓度赤霉素对果蔗产量和品质的影响研究初报[J].广西蔗糖, 2007,49(4):8-13, 27.
[4] Lu J J, Lei C Y, Zha L S, et al. A study on the application and residues of plant growth regulators in the fruit sugarcane grown in the sub-suitable region[J]. Journal of Agricultural Science, 2010,2(4):254-256.
[5] El-Lattief E A A, Bekheet M A. Quantitative and qualitative attributes of three sugarcane varieties as influenced by foliar spray of some growth regulators under upper Egypt conditions[J]. Sugar Tech, 2012, 14(4):345-350.
[6]吴建明,李杨瑞,王爱勤,等.赤霉素处理对甘蔗节间伸长及产质量的影响[J].中国糖业, 2010(4):24-26.
[7]吴建明,李杨瑞,王爱勤,等.赤霉素诱导甘蔗节间伸长的效应与相关酶活性的关系[J].西北植物学报,2010, 30(5):962-967.
[8]吴建明,李杨瑞,王爱勤,等.赤霉素诱导甘蔗节间伸长与内源激素变化的关系[J].热带作物学报,2009, 30(10):1 452-1 457.
[9]张国平, Chuula Argent.赤霉素与矮壮素或多效唑配合使用对小麦生育和代谢的影响[J].浙江农业学报,1992, 4(1):15-19.
[10]陈晓光,王振林,彭佃亮,等.种植密度与喷施多效唑对冬小麦抗倒伏能力和产量的影响[J].应用生态学报, 2011, 22(6):1 465-1 470.
[11]禤维言,张涛,黄永禄,等.喷施多效唑对甜高粱生长及生理特性的影响[J].作物杂志, 2011(5):73-76.
[12]班德宇,陈小英,黄诚梅.赤霉素与多效唑对甘蔗愈伤再生苗生长的影响[J].中国糖料, 2015, 37(5):20-23.
[13]游建华,何为中,曾慧,等.谈脱毒健康种苗在广西甘蔗生产的应用及效益展望[J].甘蔗糖业, 2001(1):13-17.
[14] Lee T S G. Multiplication of sugarcane by apex culture[J]. Turrialba, 1986, 36:231-235.
[15] Lee T S G. Micropropagation of sugarcane(Saccharum spp.)[J]. Plant Cell, Tissue and Organ Culture, 1987, 10:47-55.
[16] Garcia R, Cidade D, Castellar A, et al. In vitro morphogenesis patterns from shoot apices of sugarcane are determined by light and type of growth regulator[J]. Plant Cell, Tissue and Organ Culture, 2007, 90(2):181-190.
[17]李松,余坤兴,刘丽敏,等.甘蔗茎尖胚状体脱毒苗快繁技术研究[J].中国糖料, 2010(4):1-5, 8.
[18]李松,余坤兴,刘丽敏,等.甘蔗茎尖胚状体脱毒苗快繁技术研究[J].江苏农业科学, 2011, 39(2):83-87.
[19]何钟佩.农作物化学控制实验指导[M].北京:北京农业大学出版社, 1993:60-68.
[20] Pimenta Lange M J, Lange T. Gibberellin biosynthesis and the regulation of plant de velopment[J]. Plant Biology(Stuttg), 2006, 8(3):281-290.
[21]尚玉磊,李春喜,邵云,等.禾本科主要作物生育初期内源激素动态及其作用的比较[J].华北农学报,2004, 19(4):47-50.
[22] Hoffmann-Benning S, Kende H. On the role of abscisic acid and gibberellin in the regulation of growth in rice[J]. Plant Physiology, 1992, 99(3):1 156-1 161.
[23]叶梅荣,朱昌华,甘立军,等.激素间相互作用对植物茎伸长生长的调控综述[J].中国农学通报,2007, 23(4):228-231.
[24]房增国,赵秀芬,高祖明.多效唑对苗期稻麦叶片内源游离态激素含量的影响[J].莱阳农学院学报,2005, 22(2):101-104.
[25]陈俊伟,谢鸣,秦巧平.植物糖信号与激素信号之间的联系[J].植物生理学通讯, 2005, 41(3):279-285.
[26]朱云集,李向阳,郭天财,等.不同冠温特征冬小麦籽粒灌浆过程中内源激素含量的变化[J].植物生理学通讯, 2005, 41(6):720-724.
[27]王忠,王三根,李合生.植物生理学[M].北京:中国农业出版社, 2000:264-311.
[28]樊金娟,李雪梅,阮燕晔,等.杂交水稻及其亲本灌浆过程中内源激素含量的变化[J].植物生理学通讯,2004 40(2):146-148.
[29]李春俭.乙烯在豌豆中植株顶端优势中的作用[J].植物生理学报, 1997, 23(3):283-287.
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