喷施赤霉素对欧李果实抗氧化能力的影响
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摘要
为给调控欧李果实品质提供参考,选用‘农大6号’和‘农大7号’2个欧李品种果实作为试验材料,分别喷施质量浓度为50、100和150 mg/L的赤霉素,测定总酚和类黄酮含量及抗氧化指标。结果表明:赤霉素处理抑制了‘农大6号’欧李果实总酚和类黄酮的合成;100 mg/L的赤霉素处理促进了‘农大7号’欧李果实总酚和类黄酮的合成,50和150 mg/L的赤霉素处理抑制了‘农大7号’欧李果实总酚和类黄酮的合成。赤霉素对‘农大6号’欧李果实总酚和类黄酮的抑制作用要大于‘农大7号’。3个抗氧化指标(ABTS、FRAP和DPPH)中,‘农大6号’欧李果实的ABTS受赤霉素作用的影响最小,‘农大7号’是DPPH受赤霉素作用的影响最小。
In order to provide some references for regulating and controlling fruit quality in Cerasus humilis, using fruits from two cultivars of ‘Nongda No.6' and ‘Nongda No.7' as test materials, different mass concentrations of GA3(50, 100 and 150 mg/L) were sprayed, and total phenol content, flavonoid content and antioxidant indexes were determined. The results showed that GA3 treatments inhibited synthesis of total phenol and flavonoid in ‘Nongda No.6' fruit; 100 mg/L GA3 treatment promoted synthesis of total phenol and flavonoid in ‘Nongda No.7' fruit, but 50 and 150 mg/L GA3 treatments inhibited synthesis of total phenol and flavonoid in ‘Nongda No.7' fruit. Inhibitory effect of GA3 on total phenol and flavonoid in ‘Nongda No.6' fruit was greater than that in ‘Nongda No.7' fruit. Among three antioxidant indexes(ABTS,FRAP and DPPH), GA3 had the least effects on ABTS of ‘Nongda No.6' fruit, while GA3 had the least effects on DPPH of‘Nongda No.7' fruit.
In order to provide some references for regulating and controlling fruit quality in Cerasus humilis, using fruits from two cultivars of ‘Nongda No.6' and ‘Nongda No.7' as test materials, different mass concentrations of GA3(50, 100 and 150 mg/L) were sprayed, and total phenol content, flavonoid content and antioxidant indexes were determined. The results showed that GA3 treatments inhibited synthesis of total phenol and flavonoid in ‘Nongda No.6' fruit; 100 mg/L GA3 treatment promoted synthesis of total phenol and flavonoid in ‘Nongda No.7' fruit, but 50 and 150 mg/L GA3 treatments inhibited synthesis of total phenol and flavonoid in ‘Nongda No.7' fruit. Inhibitory effect of GA3 on total phenol and flavonoid in ‘Nongda No.6' fruit was greater than that in ‘Nongda No.7' fruit. Among three antioxidant indexes(ABTS,FRAP and DPPH), GA3 had the least effects on ABTS of ‘Nongda No.6' fruit, while GA3 had the least effects on DPPH of‘Nongda No.7' fruit.
引文
[1]王鹏飞.欧李果实转录组测序及苹果酸积累关键酶基因的克隆与表达分析[D].晋中:山西农业大学,2015.
[2]薛莞莞,龚荣高,丁建林,等.赤霉素喷施对红灯甜樱桃果实品质及解剖结构的影响[J].浙江农业学报,2018,30(6):978-984.
[3]申瑞雪,潜伟平,刘江华,等.不同温度下赤霉素处理对乌饭树与短尾越橘种子发芽的影响[J].经济林研究,2012,30(4):13-18.
[4]彭映辉,曾冬琴,陈飞飞,等.赤霉素及多效唑对3种草本花卉花期与株高的影响[J].中南林业科技大学学报,2007,27(4):100-103.
[5]唐辉,韦霄,梁惠凌,等.外源激素对银杏高位嫁接及其枝梢生长的影响[J].中南林业科技大学学报,2007,27(1):85-87.
[6]杨文莉,周伟权,赵世荣,等.GA3对轮台白杏坐果及果实品质的影响[J].新疆农业科学,2017,54(4):597-604.
[7]张宇,黄国弟,庞新华,等.赤霉素对台农16号菠萝生长及果实品质的影响[J].经济林研究,2014,32(4):110-113.
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[9]BALASUNDRAM N,SUNDRAM K,SAMMAN S.Phenolic compounds in plants and agri-industrial by-products:antioxidant activity,occurrence,and potential uses[J].Food Chemistry,2006,99(1):191-203.
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[11]周明,沈勇根,朱丽琴,等.植物黄酮化合物生物合成、积累及调控的研究进展[J].食品研究与开发,2016,37(18):216-221.
[12]余晓红,朱雪梅,李凤伟,等.芦苇叶黄酮类提取物体内体外抗氧化性研究[J].食品科学,2015,36(1):209-213.
[13]赖建平,顾采琴,朱冬雪,等.香蕉果皮黄酮及与丙酸、茶多酚复配剂的抑菌防腐作用[J].食品科学,2012,33(13):53-57.
[14]李富华,刘冬,明建.苦荞麸皮黄酮抗氧化及抗肿瘤活性[J].食品科学,2014,35(7):58-63.
[15]LUNET N,LACERDA-VIEIRA A,BARROS H.Fruit and vegetables consumption and gastric cancer:a systematic review and meta-analysis of cohort studies[J].Nutrition and Cancer,2005,53(1):1-10.
[16]白东海.欧李果实类黄酮物质提取、组分鉴定及抗氧化能力研究[D].晋中:山西农业大学,2015.
[17]LI W,LI O,ZHANG A,et al.Genotypic diversity of phenolic compounds and antioxidant capacity of Chinese dwarf cherry(Cerasus humilis(Bge)Sok)in China[J].Scientia Horticulturae,2014,175(1):208-213.
[18]蔡文国,吴卫,邵金凤,等.Folin-Ciocalteu法测定鱼腥草多酚的含量[J].食品科学,2010,31(14):201-204.
[19]YIN R,HAN K,HELLER W,et al.Kaempferol 3-O-rhamnoside-7-O-rhamnoside is an endogenous flavonol inhibitor of polar auxin transport in Arabidopsis shoots[J].New Phytologist,2014,201(2):466-475.
[20]BRUNETTI C,DI FERDINANDO M,FINI A,et al.Flavonoids as antioxidants and developmental regulators:relative significance in plants and humans[J].International Journal of Molecular Sciences,2013,14(2):3540-3555.
[21]BETTINA D,ANNE-CHRISTIN W,BERND S.The occurrence of flavonoids and related compounds in flower sections of Papaver nudicaule[J].Plants,2016,5(2):28.
[22]WANG T,LIU Y,LI X,et al.Isoflavones from green vegetable soya beans and their antimicrobial and antioxidant activities[J].Journal of the Science of Food and agriculture,2018,98:2043-2047.
[23]OGAH O,WATKINS C S,UBI B E,et al.Phenolic compounds in Rosaceae fruit and nut crops[J].Journal of Agricultural and Food Chemistry,2014,62(39):9369-9386.
[24]李晓英,薛梅,樊汶樵,等.不同生长期蓝莓叶多酚物质含量及抗氧化活性研究[J].经济林研究,2018,36(4):40-46.
[25]苏艳丽,杨健,田永真,等.梨果实发育过程中褐变相关生理指标的变化[J].果树学报,2018,35(增刊1):118-124.
[26]胡会刚,杨转英,庞振才,等.番石榴发育期生物活性物质研究[J].热带作物学报,2018,39(10):1940-1946.
[27]黄春辉,廖光联,谢敏,等.不同猕猴桃品种果实发育过程中总酚和类黄酮含量及抗氧化活性的动态变化[J].果树学报,2019,36(2):174-184.
[28]黄先忠,蒋才富,廖立力,等.赤霉素作用机理的分子基础与调控模式研究进展[J].植物学通报,2006,23(5):499-510.
[29]乔献丽,蒋曙光,吕晓芬,等.激素对转基因雪莲毛状根植株再生及类黄酮产生的影响[J].生物工程学报,2011,27(1):69-75.
[30]KIM Y.Gibberellic acid regulates flavonoid 3′,5′-hydroxylase gene transcription in the corolla of Gentiana scabra[J].Journal of Plant Biology,1999,42(4):253-258.
[31]程水源,王燕,李俊凯,等.内源激素含量与银杏叶中类黄酮含量的关系[J].林业科学,2004,40(6):45-49.
[32]孙莹,侯智霞,苏淑钗,等.ABA、GA3和NAA对蓝莓生长发育和花青苷积累的影响[J].华南农业大学学报,2013,34(1):6-11.
[33]ILAN A,DOUGALL D K.Effects of gibberellic acid and uniconazole on the activities of some enzymes of anthocyanin biosynthesis in carrot cell cultures[J].Journal of Plant Growth Regulation,1994,13(4):213-219.
[2]薛莞莞,龚荣高,丁建林,等.赤霉素喷施对红灯甜樱桃果实品质及解剖结构的影响[J].浙江农业学报,2018,30(6):978-984.
[3]申瑞雪,潜伟平,刘江华,等.不同温度下赤霉素处理对乌饭树与短尾越橘种子发芽的影响[J].经济林研究,2012,30(4):13-18.
[4]彭映辉,曾冬琴,陈飞飞,等.赤霉素及多效唑对3种草本花卉花期与株高的影响[J].中南林业科技大学学报,2007,27(4):100-103.
[5]唐辉,韦霄,梁惠凌,等.外源激素对银杏高位嫁接及其枝梢生长的影响[J].中南林业科技大学学报,2007,27(1):85-87.
[6]杨文莉,周伟权,赵世荣,等.GA3对轮台白杏坐果及果实品质的影响[J].新疆农业科学,2017,54(4):597-604.
[7]张宇,黄国弟,庞新华,等.赤霉素对台农16号菠萝生长及果实品质的影响[J].经济林研究,2014,32(4):110-113.
[8]赵洪芝.拟南芥植物代谢组学及金银花代谢产物抗炎活性研究[D].天津:南开大学,2012.
[9]BALASUNDRAM N,SUNDRAM K,SAMMAN S.Phenolic compounds in plants and agri-industrial by-products:antioxidant activity,occurrence,and potential uses[J].Food Chemistry,2006,99(1):191-203.
[10]MORIMOTO M,TANIMOTO K,NAKANO S,et al.Insect antifeedant activity of flavones and chromones against Spodoptera litura[J].J Agric Food Chem,2003,51(2):389-393.
[11]周明,沈勇根,朱丽琴,等.植物黄酮化合物生物合成、积累及调控的研究进展[J].食品研究与开发,2016,37(18):216-221.
[12]余晓红,朱雪梅,李凤伟,等.芦苇叶黄酮类提取物体内体外抗氧化性研究[J].食品科学,2015,36(1):209-213.
[13]赖建平,顾采琴,朱冬雪,等.香蕉果皮黄酮及与丙酸、茶多酚复配剂的抑菌防腐作用[J].食品科学,2012,33(13):53-57.
[14]李富华,刘冬,明建.苦荞麸皮黄酮抗氧化及抗肿瘤活性[J].食品科学,2014,35(7):58-63.
[15]LUNET N,LACERDA-VIEIRA A,BARROS H.Fruit and vegetables consumption and gastric cancer:a systematic review and meta-analysis of cohort studies[J].Nutrition and Cancer,2005,53(1):1-10.
[16]白东海.欧李果实类黄酮物质提取、组分鉴定及抗氧化能力研究[D].晋中:山西农业大学,2015.
[17]LI W,LI O,ZHANG A,et al.Genotypic diversity of phenolic compounds and antioxidant capacity of Chinese dwarf cherry(Cerasus humilis(Bge)Sok)in China[J].Scientia Horticulturae,2014,175(1):208-213.
[18]蔡文国,吴卫,邵金凤,等.Folin-Ciocalteu法测定鱼腥草多酚的含量[J].食品科学,2010,31(14):201-204.
[19]YIN R,HAN K,HELLER W,et al.Kaempferol 3-O-rhamnoside-7-O-rhamnoside is an endogenous flavonol inhibitor of polar auxin transport in Arabidopsis shoots[J].New Phytologist,2014,201(2):466-475.
[20]BRUNETTI C,DI FERDINANDO M,FINI A,et al.Flavonoids as antioxidants and developmental regulators:relative significance in plants and humans[J].International Journal of Molecular Sciences,2013,14(2):3540-3555.
[21]BETTINA D,ANNE-CHRISTIN W,BERND S.The occurrence of flavonoids and related compounds in flower sections of Papaver nudicaule[J].Plants,2016,5(2):28.
[22]WANG T,LIU Y,LI X,et al.Isoflavones from green vegetable soya beans and their antimicrobial and antioxidant activities[J].Journal of the Science of Food and agriculture,2018,98:2043-2047.
[23]OGAH O,WATKINS C S,UBI B E,et al.Phenolic compounds in Rosaceae fruit and nut crops[J].Journal of Agricultural and Food Chemistry,2014,62(39):9369-9386.
[24]李晓英,薛梅,樊汶樵,等.不同生长期蓝莓叶多酚物质含量及抗氧化活性研究[J].经济林研究,2018,36(4):40-46.
[25]苏艳丽,杨健,田永真,等.梨果实发育过程中褐变相关生理指标的变化[J].果树学报,2018,35(增刊1):118-124.
[26]胡会刚,杨转英,庞振才,等.番石榴发育期生物活性物质研究[J].热带作物学报,2018,39(10):1940-1946.
[27]黄春辉,廖光联,谢敏,等.不同猕猴桃品种果实发育过程中总酚和类黄酮含量及抗氧化活性的动态变化[J].果树学报,2019,36(2):174-184.
[28]黄先忠,蒋才富,廖立力,等.赤霉素作用机理的分子基础与调控模式研究进展[J].植物学通报,2006,23(5):499-510.
[29]乔献丽,蒋曙光,吕晓芬,等.激素对转基因雪莲毛状根植株再生及类黄酮产生的影响[J].生物工程学报,2011,27(1):69-75.
[30]KIM Y.Gibberellic acid regulates flavonoid 3′,5′-hydroxylase gene transcription in the corolla of Gentiana scabra[J].Journal of Plant Biology,1999,42(4):253-258.
[31]程水源,王燕,李俊凯,等.内源激素含量与银杏叶中类黄酮含量的关系[J].林业科学,2004,40(6):45-49.
[32]孙莹,侯智霞,苏淑钗,等.ABA、GA3和NAA对蓝莓生长发育和花青苷积累的影响[J].华南农业大学学报,2013,34(1):6-11.
[33]ILAN A,DOUGALL D K.Effects of gibberellic acid and uniconazole on the activities of some enzymes of anthocyanin biosynthesis in carrot cell cultures[J].Journal of Plant Growth Regulation,1994,13(4):213-219.
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