盐碱地不同土地利用方式下土壤细菌群落结构多样性差异分析
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摘要
为了研究盐碱地不同土地利用方式下土壤细菌群落结构特征分布,利用高通量测序技术,对黄河三角洲地区的耕地、园地和荒地中3个不同深度的土壤细菌群落结构多样性进行了研究.结果表明:①荒地中的土壤细菌群落丰富度和多样性均大于其他土地利用类型.②相同土地利用类型的细菌群落结构相似度较高,而不同土地利用类型的细菌群落结构有一定的差别.③细菌群落在门类水平上达到20门以上,优势菌种均以放线菌门、变形菌门、酸杆菌门、绿弯菌门及浮霉菌门为主,相对丰度约为69%～83%;属类水平的细菌群落达230多属,酸杆菌属在土壤中的相对丰度最高,为5.76%.④土壤有效磷、速效钾和有机质含量与土壤细菌多样性指数呈显著相关性.
In order to study the effects of different land use patterns on the distribution of bacterial community structure in saline-alkaline land, high throughput sequencing technology was used to study the characteristics of soil bacterial community at three different depths in cultivated land, garden land and wasteland in the Yellow River delta region.The results show that: ①The richness and diversity of soil bacterial community in wasteland are higher than the other land use types.②The bacteria community structure of the same land use type is similar, but the bacteria community structure of different land use types is to some extent different. ③The bacterial community reaches more than 20 gates at the phylotypal level, and the dominant species are Actinomycetes, Proteobacteria, Acidomycetes, Chlorobacteria, and Pondiomycetes, with a relative abundance of about 69%～83%. The bacteria community of genera level reaches more than 230 genera, and the relative abundance of Acidophilus in soil is up to 5.76%. ④The contents of available phosphorous, available kalium and organic matter in soil are significantly correlated with soil bacterial diversity index.
In order to study the effects of different land use patterns on the distribution of bacterial community structure in saline-alkaline land, high throughput sequencing technology was used to study the characteristics of soil bacterial community at three different depths in cultivated land, garden land and wasteland in the Yellow River delta region.The results show that: ①The richness and diversity of soil bacterial community in wasteland are higher than the other land use types.②The bacteria community structure of the same land use type is similar, but the bacteria community structure of different land use types is to some extent different. ③The bacterial community reaches more than 20 gates at the phylotypal level, and the dominant species are Actinomycetes, Proteobacteria, Acidomycetes, Chlorobacteria, and Pondiomycetes, with a relative abundance of about 69%～83%. The bacteria community of genera level reaches more than 230 genera, and the relative abundance of Acidophilus in soil is up to 5.76%. ④The contents of available phosphorous, available kalium and organic matter in soil are significantly correlated with soil bacterial diversity index.
引文
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刘开辉,张波,丁小维,等.2018.基于高通量测序的花马盐湖原核微生物及其耐盐基因[J].微生物学报,58(10):1743-1753
刘灵芝,秦嗣军,吕德国,等.2013.樱桃园土壤优势放线菌的分离及其促长功能研究[J].中国农业科学,46(6):1221-1229
刘旻霞,李瑞,张灿,等.2018.兰州市南山季节性土壤微生物特征及影响因素[J].中国环境科学,38(7):2722-2730
刘兴华,章海波,李远,等.2018.黄河三角洲滩涂-湿地-旱地土壤团聚体有机质组分变化规律[J].土壤学报,56(2): 375-386
缪凌,董建国,汪有科,等.2016.黄土丘陵区不同土地利用类型下的深层土壤水分变化特征[J].水土保持研究,29(2):13-18
Lu X,Nicol G W,Neufeld J D.2018.Differential responses of soil ammonia-oxidizing archaea and bacteria to temperature and depth under two different land uses[J].Soil Biology and Biochemistry,120:272-282
Lúcio W D S,Lacerda C F D,Mendes Filho P F,et al.2013.Crescimento e respostas fisiológicas do meloeiro inoculado com fungos micorrízicos arbusculares sob estresse salino[J].Semina:Ciências Agrárias,34(4):1587-1602
Lüneberg K,Schneider D,Siebe C,et al.2018.Drylands soil bacterial community is affected by land use change and different irrigation practices in the Mezquital Valley,Mexico[J].Scientific Reports,8(1):1-15
Ma B,Zhao K,Lv X,et al.2018.Genetic correlation network prediction of forest soil microbial functional organization[J].The ISME Journal,12(10):2492
Mitchell J S,Ruess R W.2009.N2 fixing alder (Alnus viridis spp.fruticosa) effects on soil properties across a secondary successional chronosequence in interior Alaska[J].Biogeochemistry,95(2/3):215-229
Ndour N Y B,Achouak W,Christen R,et al.2008.Characteristics of microbial habitats in a tropical soil subject to different fallow management[J].Applied Soil Ecology,38(1):51-61
Ottinger M,Kuenzer C,Liu G,et al.2013.Monitoring land cover dynamics in the Yellow River Delta from 1995 to 2010 based on Landsat 5 TM[J].Applied Geography,44:53-68
Pattanop K,Tanaporn U,Ukrit R.2011.Insights into the phylogeny and metabolic potential of a primary tropical peat swamp forest microbial community by metagenomic analysis[J].Microbial Ecology,61:518-528
秦红,李昌晓,任庆水.2017.不同土地利用方式对三峡库区消落带土壤细菌和真菌多样性的影响[J].生态学报,37(10):3494-3504
Qadir M,Tubeileh A,Akhtar J,et al.2008.Productivity enhancement of salt-affected environments through crop diversification[J].Land Degradation & Development,19(4):429-453
Ramirez K S,Craine J M,Fierer N.2012.Consistent effects of nitrogen amendments on soil microbial communities and processes across biomes[J].Global Change Biology,18(6):1918-1927
Sait M,Davis K E R,Janssen P H.2006.Effect of pH on isolation and distribution of members of subdivision 1 of the phylum Acidobacteria occurring in soil[J].Applied and Environmental Microbiology,72(3):1852-1857
万盼,胡艳波,张弓乔,等.2018.甘肃小陇山油松与柴胡栽培土壤细菌群落特征[J].生态学报,38(17):1-8
王颖,宗宁,何念鹏,等.2018.青藏高原高寒草甸不同海拔梯度下土壤微生物群落碳代谢多样性[J].生态学报,38(16):1-8
王影,张志明,李晓慧,等.2013.土地利用方式对土壤细菌、泉古菌和氨氧化古菌丰度的影响[J].生态学杂志,32(11):2931-2936
Wang X,Wang Q,Guo X,et al.2015.Functional genomic analysis of Hawaii marine metagenomes[J].Science Bulletin,60(3):348-355
夏昕,石坤,黄欠如,等.2015.长期不同施肥条件下红壤性水稻土微生物群落结构的变化[J].土壤学报,52(3):697-705
徐志霞,王璇,李慧敏,等.2018.不同林龄木麻黄林地土壤细菌及与土壤因子的相关性分析[J].基因组学与应用生物学,37(2):780-788
Xu M,Li X,Cai X,et al.2017.Land use alters arbuscular mycorrhizal fungal communities and their potential role in carbon sequestration on the Tibetan Plateau[J].Scientific Reports,7(1):1-11
杨劲松.2008.中国盐渍土研究的发展历程与展望[J].土壤学报,45(5):837-845
Yang J,Zhang J,Yu H.2014.Community composition and cellulase activity of cellulolytic bacteria from forest soils planted with broad-leaved deciduous and evergreen trees[J].Applied Microbiology and Biotechnology,98(3):1449-1458
张媚,林马水,曹秀秀,等.2018.不同经营模式山核桃林地土壤pH值、养分与细菌多样性的差异[J].生物多样性,26(6):611-619
张双双,靳振江,贾远航,等.2019.岩溶地区不同土地利用方式土壤固碳细菌群落结构特征[J].环境科学,40(1):1-12
张彦军.2018.环境因子对土壤微生物呼吸及其温度敏感性变化特征的影响[J].环境科学,39(6):1-21
Zhang S,Fan C,Wang Y,et al.2018.Salt-tolerant and plant growth-promoting bacteria isolated from high-yield paddy soil[J].Can J Microbiol,:1-33
包明,何红霞,马小龙,等.2018.化学氮肥与绿肥对麦田土壤细菌多样性和功能的影响[J].土壤学报,55(3):734-743
Bell C W,Asao S,Calderon F,et al.2015.Plant nitrogen uptake drives rhizosphere bacterial community assembly during plant growth[J].Soil Biology and Biochemistry,85:170-182
Bowman K,Nobre M,Da M C.2013.Dehalogenimonas alkenigignens sp.nov.a chlorinated-alkane-dehalogenating bacterium isolated from groundwater[J].International Journal of Systematic & Evolutionary Microbiology,64(4):1492-1498
Chandrasekaran M,Boughattas S,Hu S,et al.2014.A meta-analysis of arbuscular mycorrhizal effects on plants grown under salt stress[J].Mycorrhiza,24(8):611-625
Chatterjee P,Samaddar S,Anandham R,et al.2017.Beneficial soil bacterium pseudomonas frederiksbergensis OS261 augments salt tolerance and promotes red pepper plant growth[J].Frontiers in Plant Science,8:1-9
陈重军,张海芹,汪瑶琪,等.2016.基于高通量测序的ABR厌氧氨氧化反应器各隔室细菌群落特征分析[J].环境科学,37(7):2652-2658
Chen Y,Wen Y,Tang Z,et al.2015.Effects of plant biomass on bacterial community structure in constructed wetlands used for tertiary wastewater treatment[J].Ecological Engineering,84:38-45
Eichorst S A,Breznak J A,Schmidt T M.2007.Isolation and characterization of soil bacteria that define terriglobus gen.nov.,in the phylum acidobacteria[J].Applied and Environmental Microbiology,73(8):2708-2717
冯晨,孙占祥,王宝荣,等.2018.辽西地区覆膜条件对不同土层土壤氮磷钾累积量的影响[J].中国土壤与肥料,(5):129-133
高鹏飞,姚国强,赵树平,等.2014.乳酸菌在农产品种植及其质量安全中的研究进展[J].中国农业科技导报,16(6):143-148
Gomez E,Pioli R,Conti M.2007.Fungal abundance and distribution as influenced by clearing and land use in a vertic soil of Argentina[J].Biology and Fertility of Soils,43(3):373-377
孔涛,张德胜,徐慧,等.2014.盐碱地及其改良过程中土壤微生物生态特征研究进展[J].土壤,46(4):581-588
李倩,马琨,冶秀香,等.2018.不同培肥方式对土壤有机碳与微生物群落结构的影响[J].中国生态农业学报,26(12):1-8
梁志婷,邓建强,王自奎,等.2017.陇东旱塬区不同粮草轮作模式下土壤细菌群落组成特征[J].草业学报,26(8):180-191
刘开辉,张波,丁小维,等.2018.基于高通量测序的花马盐湖原核微生物及其耐盐基因[J].微生物学报,58(10):1743-1753
刘灵芝,秦嗣军,吕德国,等.2013.樱桃园土壤优势放线菌的分离及其促长功能研究[J].中国农业科学,46(6):1221-1229
刘旻霞,李瑞,张灿,等.2018.兰州市南山季节性土壤微生物特征及影响因素[J].中国环境科学,38(7):2722-2730
刘兴华,章海波,李远,等.2018.黄河三角洲滩涂-湿地-旱地土壤团聚体有机质组分变化规律[J].土壤学报,56(2): 375-386
缪凌,董建国,汪有科,等.2016.黄土丘陵区不同土地利用类型下的深层土壤水分变化特征[J].水土保持研究,29(2):13-18
Lu X,Nicol G W,Neufeld J D.2018.Differential responses of soil ammonia-oxidizing archaea and bacteria to temperature and depth under two different land uses[J].Soil Biology and Biochemistry,120:272-282
Lúcio W D S,Lacerda C F D,Mendes Filho P F,et al.2013.Crescimento e respostas fisiológicas do meloeiro inoculado com fungos micorrízicos arbusculares sob estresse salino[J].Semina:Ciências Agrárias,34(4):1587-1602
Lüneberg K,Schneider D,Siebe C,et al.2018.Drylands soil bacterial community is affected by land use change and different irrigation practices in the Mezquital Valley,Mexico[J].Scientific Reports,8(1):1-15
Ma B,Zhao K,Lv X,et al.2018.Genetic correlation network prediction of forest soil microbial functional organization[J].The ISME Journal,12(10):2492
Mitchell J S,Ruess R W.2009.N2 fixing alder (Alnus viridis spp.fruticosa) effects on soil properties across a secondary successional chronosequence in interior Alaska[J].Biogeochemistry,95(2/3):215-229
Ndour N Y B,Achouak W,Christen R,et al.2008.Characteristics of microbial habitats in a tropical soil subject to different fallow management[J].Applied Soil Ecology,38(1):51-61
Ottinger M,Kuenzer C,Liu G,et al.2013.Monitoring land cover dynamics in the Yellow River Delta from 1995 to 2010 based on Landsat 5 TM[J].Applied Geography,44:53-68
Pattanop K,Tanaporn U,Ukrit R.2011.Insights into the phylogeny and metabolic potential of a primary tropical peat swamp forest microbial community by metagenomic analysis[J].Microbial Ecology,61:518-528
秦红,李昌晓,任庆水.2017.不同土地利用方式对三峡库区消落带土壤细菌和真菌多样性的影响[J].生态学报,37(10):3494-3504
Qadir M,Tubeileh A,Akhtar J,et al.2008.Productivity enhancement of salt-affected environments through crop diversification[J].Land Degradation & Development,19(4):429-453
Ramirez K S,Craine J M,Fierer N.2012.Consistent effects of nitrogen amendments on soil microbial communities and processes across biomes[J].Global Change Biology,18(6):1918-1927
Sait M,Davis K E R,Janssen P H.2006.Effect of pH on isolation and distribution of members of subdivision 1 of the phylum Acidobacteria occurring in soil[J].Applied and Environmental Microbiology,72(3):1852-1857
万盼,胡艳波,张弓乔,等.2018.甘肃小陇山油松与柴胡栽培土壤细菌群落特征[J].生态学报,38(17):1-8
王颖,宗宁,何念鹏,等.2018.青藏高原高寒草甸不同海拔梯度下土壤微生物群落碳代谢多样性[J].生态学报,38(16):1-8
王影,张志明,李晓慧,等.2013.土地利用方式对土壤细菌、泉古菌和氨氧化古菌丰度的影响[J].生态学杂志,32(11):2931-2936
Wang X,Wang Q,Guo X,et al.2015.Functional genomic analysis of Hawaii marine metagenomes[J].Science Bulletin,60(3):348-355
夏昕,石坤,黄欠如,等.2015.长期不同施肥条件下红壤性水稻土微生物群落结构的变化[J].土壤学报,52(3):697-705
徐志霞,王璇,李慧敏,等.2018.不同林龄木麻黄林地土壤细菌及与土壤因子的相关性分析[J].基因组学与应用生物学,37(2):780-788
Xu M,Li X,Cai X,et al.2017.Land use alters arbuscular mycorrhizal fungal communities and their potential role in carbon sequestration on the Tibetan Plateau[J].Scientific Reports,7(1):1-11
杨劲松.2008.中国盐渍土研究的发展历程与展望[J].土壤学报,45(5):837-845
Yang J,Zhang J,Yu H.2014.Community composition and cellulase activity of cellulolytic bacteria from forest soils planted with broad-leaved deciduous and evergreen trees[J].Applied Microbiology and Biotechnology,98(3):1449-1458
张媚,林马水,曹秀秀,等.2018.不同经营模式山核桃林地土壤pH值、养分与细菌多样性的差异[J].生物多样性,26(6):611-619
张双双,靳振江,贾远航,等.2019.岩溶地区不同土地利用方式土壤固碳细菌群落结构特征[J].环境科学,40(1):1-12
张彦军.2018.环境因子对土壤微生物呼吸及其温度敏感性变化特征的影响[J].环境科学,39(6):1-21
Zhang S,Fan C,Wang Y,et al.2018.Salt-tolerant and plant growth-promoting bacteria isolated from high-yield paddy soil[J].Can J Microbiol,:1-33
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