不同光质条件及NH_4~+-N、Cu~(2+)浓度对栅藻处理沼液的影响
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摘要
将筛选所得耐污能力强的栅藻作为研究对象,研究不同光质条件对栅藻处理沼液的影响,并且以实际沼液废水中NH_4~+-N、Cu2+浓度为参照设置不同浓度,分别考察NH_4~+-N、Cu2+对栅藻生长的影响。结果表明:在白光、蓝光、红光3种光质下,栅藻生物产率分别是0.21、0.04、0.03 g·L~(-1)·d~(-1),白光条件下栅藻生长相对较好。50 mg·L-1低浓度NH_4~+-N下栅藻生长较好,其生物产率优于BG 11培养基,分别为0.20、0.18 g·L~(-1)·d~(-1);500、2000 mg·L-1高浓度NH_4~+-N下,藻细胞生长缓慢,生物产率仅为0.12、0.11 g·L~(-1)·d~(-1)。在Cu2+浓度分别为0.5、1.0、2.0 mg·L-1的培养液中,藻细胞生物产率分别为0.18、0.15、0.13 g·L~(-1)·d~(-1)。一定浓度NH_4~+-N存在下,栅藻能耐受较高的Cu2+浓度。
In this study, a strain of Scendesmus dimorphus with strong anti-fouling property was selected to investigate the effects of light qualities on the growth of Scendesmus dimorphus to treat swine wastewater. The growth of the algae based on standard concentrations of ammonia nitrogen and copper in swine wastewater was studied. The results showed that the growth of algal biomass in white light was better than that in blue and red light, with biomass productivity of 0.21, 0.04 g·L~(-1)·d~(-1), and 0.03 g·L~(-1)·d~(-1), respectively. The growth of the algae at low ammonia nitrogen concentration of 50 mg·L-1was similar to that of the control, with biomass productivity of 0.20 g·L~(-1)·d~(-1)and 0.18 g·L~(-1)·d~(-1), respectively. However, the growth of the algae was influenced by high concentrations of ammonia nitrogen(500 mg·L-1and 2000mg·L-1)with biomass productivity of 0.12 g·L~(-1)·d~(-1)and 0.11 g·L~(-1)·d~(-1), respectively. In addition, the growth of Scendesmus dimorphus was influenced by copper, with biomass productivity of 0.18, 0.15 g·L~(-1)·d~(-1), and 0.13 g·L~(-1)·d~(-1)at copper concentrations of 0.5, 1.0 mg·L-1, and2.0 mg·L-1, respectively. The algal cell can be resistant to high concentrations of copper when ammonia nitrogen co-exists in the medium.
In this study, a strain of Scendesmus dimorphus with strong anti-fouling property was selected to investigate the effects of light qualities on the growth of Scendesmus dimorphus to treat swine wastewater. The growth of the algae based on standard concentrations of ammonia nitrogen and copper in swine wastewater was studied. The results showed that the growth of algal biomass in white light was better than that in blue and red light, with biomass productivity of 0.21, 0.04 g·L~(-1)·d~(-1), and 0.03 g·L~(-1)·d~(-1), respectively. The growth of the algae at low ammonia nitrogen concentration of 50 mg·L-1was similar to that of the control, with biomass productivity of 0.20 g·L~(-1)·d~(-1)and 0.18 g·L~(-1)·d~(-1), respectively. However, the growth of the algae was influenced by high concentrations of ammonia nitrogen(500 mg·L-1and 2000mg·L-1)with biomass productivity of 0.12 g·L~(-1)·d~(-1)and 0.11 g·L~(-1)·d~(-1), respectively. In addition, the growth of Scendesmus dimorphus was influenced by copper, with biomass productivity of 0.18, 0.15 g·L~(-1)·d~(-1), and 0.13 g·L~(-1)·d~(-1)at copper concentrations of 0.5, 1.0 mg·L-1, and2.0 mg·L-1, respectively. The algal cell can be resistant to high concentrations of copper when ammonia nitrogen co-exists in the medium.
引文
[1] Chen X, Luo A C, Kuniaki S, et al. An introduction of a multi-soil-lay-ering system:A novel green technology for wastewater treatment in ru-ral areas[J]. Water and Environment Journal, 2009, 23(4):255-262.
[2] Zhao J, Zhang K, Wang F, et al. Influence of livestock wastewater irriga-tion on nitrogen compounds contents in groundwater[J]. Ecology and Environmental Sciences, 2011, 20(1):149-153.
[3]吴盼盼.基于微藻养殖的养猪沼液预处理技术研究[D].南昌:南昌大学,2015.WU Pan-pan. Study on piggery biogas slurry pretreatment based on mi-croalgae culturing[D]. Nanchang:Nanchang University, 2015.
[4] Zhu L D, Hiltunen E. Application of livestock waste compost to culti-vate microalgae for bioproducts production:A feasible framework[J]. Renewable and Sustainable Energy Reviews, 2016, 54:1285-1290.
[5]刘刈,宋立,邓良伟.我国规模化养殖场粪便污水处理利用现状及对策[J].猪业科学,2011,28(6):30-33.LIU Yi, SONG Li, DENG Liang-wei. Present situation and countermea-sures of fecal sewage treatment and utilization of large-scale farm inChina[J]. Swine Industry Science, 2011,28(6):30-33.
[6] Sturm B S M, Lamer, S L. An energy evaluation of coupling nutrient re-moval from wastewater with algal biomass production[J]. Applied Energy, 2011, 88(10):3499–3506.
[7]卫丹,万梅,刘锐,等.嘉兴市规模化养猪场沼液水质调查研究[J].环境科学, 2014, 35(7):2650-2657.WEI Dan, WAN Mei, LIU Rui, et al. Study on the quality of digestedpiggery wastewater in large-scale farms in Jiaxing[J]. Environmental Science, 2014,35(7):2650-2657.
[8] Cai T, Park S Y, Li Y. Nutrient recovery from wastewater streams by mi-croalgae:Status and prospects[J]. Renew Sust Energy Rev, 2013, 19:360-369.
[9] Kumar M S, Miao Z H, Wyatt S K. Influence of nutrient loads, feedingfrequency and inoculum source on growth of Chlorella vulgaris in di-gested piggery effluent culture medium[J]. Bioresource Technology,2010, 101(15):6012-6018.
[10] Min M, Hu B, Mohr M J, et al. Swine manure-based pilot-scale algalbiomass production system for fuel production and wastewater treat-ment:A case study[J]. Applied Biochemistry and Biotechnology, 2014,172(3):1390-1406.
[11] Deng X Y, Gao K, Zhang R C, et al. Growing Chlorella vulgaris onthermophilic anaerobic digestion swine manure for nutrient removaland biomass production[J]. Bioresource Technology, 2017, 243:417-425.
[12] Deng X Y, Gao K, Addy M, et al. Cultivation of Chlorella vulgaris onanaerobically digested swine manure with daily recycling of the post-harvest culture broth[J]. Bioresource Technology, 2017, 247:716-723.
[13]苗洪利,孙丽娜,田庆震,等. LED单色光谱及复合光谱对赤潮优势种中肋骨条藻生长的作用[J].中国海洋大学学报, 2011, 41(10):107-110.MIAO Hong-li, SUN Li-na, TIAN Qing-zhen, et al. Effects of mochro-matic and combination spectra of LED on the gowth of the Skeletonema costatum in red tide[J]. Periodical of Ocean University of China,2011, 41(10):107-110.
[14]柴雨,吴垠,赵慧慧.光质对湛江等鞭金藻生长和脂肪酸组成的影响[J].植物生理通讯, 2009, 45(6):571-574.CHAI Yu, WU Yin, ZHAO Hui-hui. Effects of light qualities ongrowth and fatty acid composition of Isochrysis zhanjiangensis Hu&Liu[J]. Plant Physiology Communications, 2009, 45(6):571-574.
[15] Fernandez E, Galvan A. Nitrate assimilation in chlamydomonas[J]. Eukaryotic Cell, 2008, 7(4):555-559.
[16]卢信,罗佳,高岩,等.畜禽养殖废水中抗生素和重金属的污染效应及其修复研究进展[J].江苏农业学报,2014, 30(3):671-681.LU Xin, LUO Jia, GAO Yan, et al. A review in ecotoxic effect of anti-biotics and heavy metals co-contamination in livestock and poultrybreeding wastewater and its remediation[J]. Jiangsu Journal of Agricultural Sciences, 2014, 30(3):671-681.
[17]王愿珠,程鹏飞,刘德富,等.生物膜贴壁培养小球藻净化猪粪沼液废水的效果[J].环境科学,2017,38(8):3354-3361.WANG Yuan-zhu, CHENG Peng-fei, LIU De-fu, et al. Purificationeffect of piggery wastewater with Chlorella pyrenoidosa by immobilizedbiofilm-attached culture[J]. Environmental Science, 2017, 38(8):3354-3361.
[18]韩军军,钟晨辉,何培民,等.不同光质LED光源对坛紫菜自由丝状体生长和生理特性的影响[J].水产学报,2017,41(2):230-239.HAN Jun-jun, ZHONG Chen-hui, HE Pei-min, et al. Effects of differ-ent light-qualities on growth and physiological characteristics of freeliving conchocelis of Pyropia haitanensis[J]. Journal of Fisheries of China, 2017,41(2):230-239.
[19] Wang C Y, Fu C C, Liu Y C. Effects of using light-emitting diodes onthe cultivation of Spirulina platensis[J]. Biochemical Engineering Journal, 2007, 37(1):21-25.
[20]韩博平,韩志国,付翔.藻类光合作用机理与模型[M].北京:科学出版社,2003.HAN Bo-ping, HAN Zhi-guo, FU Xiang. Algae photosynthesis:Mech-anisms and models[M]. Beijing:Science Press, 2003.
[21]郑洁,胡美君,郭延平.光质对植物光合作用的调控及其机理[J].应用生态学报, 2008, 19(7):1619-1624.ZHENG Jie, HU Mei-jun, GUO Yan-ping. Regulation of photosynthe-sis by light quality and its mechanism in plants[J]. Chinese Journal of Applied Ecology, 2008,19(7):1619-1624.
[22] Lu Q, Chen P, Addy M. Carbon-dependent exploration[J].Bioresource Technology, 2017, 249:9.
[23] Dai G Z,Qiu B S,Forchhammer K. Ammonium tolerance in the cyano-bacterium Synechocystis sp. strain PCC 6803 and the role of the psbAmultigene family[J]. Plant, Cell&Environment, 2014, 37(4):840-851.
[24] Ayre J M, Moheimani N R, Borowitzka M A. Growth of microalgae onundiluted anaerobic digestate of piggery effluent with high ammoniumconcentrations[J]. Algal Research, 2017, 24:218-226.
[25] Wen Y M, He Y J, Ji X W, et al. Isolation of an indigenous Chlorella vulgaris from swine wastewater and characterization of its nutrient re-moval ability in undiluted sewage[J]. Bioresource Technology, 2017,243:247-253.
[26]程鹏飞,王艳,杨期勇,等.微藻贴壁培养对沼液废水的处理效果[J].浙江农业学报, 2017, 29(9):1564-1569.CHENG Peng-fei, WANG Yan, YANG Qi-yong, et al. Purification ef-fect for swine wastewater with attached culture of microalgae[J]. Acta Agriculturae Zhejiangensis, 2017, 29(9):1564-1569.
[2] Zhao J, Zhang K, Wang F, et al. Influence of livestock wastewater irriga-tion on nitrogen compounds contents in groundwater[J]. Ecology and Environmental Sciences, 2011, 20(1):149-153.
[3]吴盼盼.基于微藻养殖的养猪沼液预处理技术研究[D].南昌:南昌大学,2015.WU Pan-pan. Study on piggery biogas slurry pretreatment based on mi-croalgae culturing[D]. Nanchang:Nanchang University, 2015.
[4] Zhu L D, Hiltunen E. Application of livestock waste compost to culti-vate microalgae for bioproducts production:A feasible framework[J]. Renewable and Sustainable Energy Reviews, 2016, 54:1285-1290.
[5]刘刈,宋立,邓良伟.我国规模化养殖场粪便污水处理利用现状及对策[J].猪业科学,2011,28(6):30-33.LIU Yi, SONG Li, DENG Liang-wei. Present situation and countermea-sures of fecal sewage treatment and utilization of large-scale farm inChina[J]. Swine Industry Science, 2011,28(6):30-33.
[6] Sturm B S M, Lamer, S L. An energy evaluation of coupling nutrient re-moval from wastewater with algal biomass production[J]. Applied Energy, 2011, 88(10):3499–3506.
[7]卫丹,万梅,刘锐,等.嘉兴市规模化养猪场沼液水质调查研究[J].环境科学, 2014, 35(7):2650-2657.WEI Dan, WAN Mei, LIU Rui, et al. Study on the quality of digestedpiggery wastewater in large-scale farms in Jiaxing[J]. Environmental Science, 2014,35(7):2650-2657.
[8] Cai T, Park S Y, Li Y. Nutrient recovery from wastewater streams by mi-croalgae:Status and prospects[J]. Renew Sust Energy Rev, 2013, 19:360-369.
[9] Kumar M S, Miao Z H, Wyatt S K. Influence of nutrient loads, feedingfrequency and inoculum source on growth of Chlorella vulgaris in di-gested piggery effluent culture medium[J]. Bioresource Technology,2010, 101(15):6012-6018.
[10] Min M, Hu B, Mohr M J, et al. Swine manure-based pilot-scale algalbiomass production system for fuel production and wastewater treat-ment:A case study[J]. Applied Biochemistry and Biotechnology, 2014,172(3):1390-1406.
[11] Deng X Y, Gao K, Zhang R C, et al. Growing Chlorella vulgaris onthermophilic anaerobic digestion swine manure for nutrient removaland biomass production[J]. Bioresource Technology, 2017, 243:417-425.
[12] Deng X Y, Gao K, Addy M, et al. Cultivation of Chlorella vulgaris onanaerobically digested swine manure with daily recycling of the post-harvest culture broth[J]. Bioresource Technology, 2017, 247:716-723.
[13]苗洪利,孙丽娜,田庆震,等. LED单色光谱及复合光谱对赤潮优势种中肋骨条藻生长的作用[J].中国海洋大学学报, 2011, 41(10):107-110.MIAO Hong-li, SUN Li-na, TIAN Qing-zhen, et al. Effects of mochro-matic and combination spectra of LED on the gowth of the Skeletonema costatum in red tide[J]. Periodical of Ocean University of China,2011, 41(10):107-110.
[14]柴雨,吴垠,赵慧慧.光质对湛江等鞭金藻生长和脂肪酸组成的影响[J].植物生理通讯, 2009, 45(6):571-574.CHAI Yu, WU Yin, ZHAO Hui-hui. Effects of light qualities ongrowth and fatty acid composition of Isochrysis zhanjiangensis Hu&Liu[J]. Plant Physiology Communications, 2009, 45(6):571-574.
[15] Fernandez E, Galvan A. Nitrate assimilation in chlamydomonas[J]. Eukaryotic Cell, 2008, 7(4):555-559.
[16]卢信,罗佳,高岩,等.畜禽养殖废水中抗生素和重金属的污染效应及其修复研究进展[J].江苏农业学报,2014, 30(3):671-681.LU Xin, LUO Jia, GAO Yan, et al. A review in ecotoxic effect of anti-biotics and heavy metals co-contamination in livestock and poultrybreeding wastewater and its remediation[J]. Jiangsu Journal of Agricultural Sciences, 2014, 30(3):671-681.
[17]王愿珠,程鹏飞,刘德富,等.生物膜贴壁培养小球藻净化猪粪沼液废水的效果[J].环境科学,2017,38(8):3354-3361.WANG Yuan-zhu, CHENG Peng-fei, LIU De-fu, et al. Purificationeffect of piggery wastewater with Chlorella pyrenoidosa by immobilizedbiofilm-attached culture[J]. Environmental Science, 2017, 38(8):3354-3361.
[18]韩军军,钟晨辉,何培民,等.不同光质LED光源对坛紫菜自由丝状体生长和生理特性的影响[J].水产学报,2017,41(2):230-239.HAN Jun-jun, ZHONG Chen-hui, HE Pei-min, et al. Effects of differ-ent light-qualities on growth and physiological characteristics of freeliving conchocelis of Pyropia haitanensis[J]. Journal of Fisheries of China, 2017,41(2):230-239.
[19] Wang C Y, Fu C C, Liu Y C. Effects of using light-emitting diodes onthe cultivation of Spirulina platensis[J]. Biochemical Engineering Journal, 2007, 37(1):21-25.
[20]韩博平,韩志国,付翔.藻类光合作用机理与模型[M].北京:科学出版社,2003.HAN Bo-ping, HAN Zhi-guo, FU Xiang. Algae photosynthesis:Mech-anisms and models[M]. Beijing:Science Press, 2003.
[21]郑洁,胡美君,郭延平.光质对植物光合作用的调控及其机理[J].应用生态学报, 2008, 19(7):1619-1624.ZHENG Jie, HU Mei-jun, GUO Yan-ping. Regulation of photosynthe-sis by light quality and its mechanism in plants[J]. Chinese Journal of Applied Ecology, 2008,19(7):1619-1624.
[22] Lu Q, Chen P, Addy M. Carbon-dependent exploration[J].Bioresource Technology, 2017, 249:9.
[23] Dai G Z,Qiu B S,Forchhammer K. Ammonium tolerance in the cyano-bacterium Synechocystis sp. strain PCC 6803 and the role of the psbAmultigene family[J]. Plant, Cell&Environment, 2014, 37(4):840-851.
[24] Ayre J M, Moheimani N R, Borowitzka M A. Growth of microalgae onundiluted anaerobic digestate of piggery effluent with high ammoniumconcentrations[J]. Algal Research, 2017, 24:218-226.
[25] Wen Y M, He Y J, Ji X W, et al. Isolation of an indigenous Chlorella vulgaris from swine wastewater and characterization of its nutrient re-moval ability in undiluted sewage[J]. Bioresource Technology, 2017,243:247-253.
[26]程鹏飞,王艳,杨期勇,等.微藻贴壁培养对沼液废水的处理效果[J].浙江农业学报, 2017, 29(9):1564-1569.CHENG Peng-fei, WANG Yan, YANG Qi-yong, et al. Purification ef-fect for swine wastewater with attached culture of microalgae[J]. Acta Agriculturae Zhejiangensis, 2017, 29(9):1564-1569.