玉米骨干自交系对除草剂苯磺隆和甲咪唑烟酸的敏感性差异
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摘要
为了在培育玉米抗除草剂突变体过程中选择合适的受体材料,以7个玉米骨干自交系C92、京724、京2416、B73、郑58、HZ178、178为材料,研究不同自交系对苯磺隆和甲咪唑烟酸的敏感性差异。测定了不同浓度除草剂处理下各自交系的初生根长抑制率、幼苗生长抑制率和乙酰乳酸合成酶(ALS)活性,结果显示,随着2种除草剂浓度的增加,各自交系的初生根长抑制率和幼苗生长抑制率明显提高,且各自交系的ALS活性存在不同程度下降趋势。7个自交系对2种除草剂的敏感性均为C92>京724>京2416>B73>郑58>HZ178>178,各自交系均对甲咪唑烟酸更敏感。综上所述,自交系C92、京724、京2416相比B73、郑58、178、HZ178对苯磺隆和甲咪唑烟酸更加敏感,适合作为培育抗除草剂突变体的受体材料。
In order to select the right receptor material during the cultivation of herbicide resistant mutants,the sensitivity of various maize inbred lines to tribenuron-methyl and imazapic was studied using seven maize backbone inbred lines,C92,Jing 724,Jing 2416,B73,Zheng 58,HZ178 and 178.We measured the inhibition rates of primary root length,seedling growth and acetolactate synthase(ALS) activity of each inbred line under different herbicide concentration.The results showed that the inhibition rates of primary root length and seedling growth of each inbred line increased obviously with the increase of the treatment concentration of two herbicides.And the ALS activity of each inbred line decreased to different degrees.The sensitivities of seven inbred lines under two herbicides were:C92>Jing 724>Jing 2416>B73>Zheng 58>HZ178>178.All the inbred lines were more sensitive to imazapic than tribenuron-methyl.To sum up,compared with B73,Zheng 58,178 and HZ178,the inbred lines C92,Jing 724 and Jing 2416 are more sensitive to tribenuron-methyl and imazapic,and suitable as receptor materials for cultivation of anti-herbicide mutant.
In order to select the right receptor material during the cultivation of herbicide resistant mutants,the sensitivity of various maize inbred lines to tribenuron-methyl and imazapic was studied using seven maize backbone inbred lines,C92,Jing 724,Jing 2416,B73,Zheng 58,HZ178 and 178.We measured the inhibition rates of primary root length,seedling growth and acetolactate synthase(ALS) activity of each inbred line under different herbicide concentration.The results showed that the inhibition rates of primary root length and seedling growth of each inbred line increased obviously with the increase of the treatment concentration of two herbicides.And the ALS activity of each inbred line decreased to different degrees.The sensitivities of seven inbred lines under two herbicides were:C92>Jing 724>Jing 2416>B73>Zheng 58>HZ178>178.All the inbred lines were more sensitive to imazapic than tribenuron-methyl.To sum up,compared with B73,Zheng 58,178 and HZ178,the inbred lines C92,Jing 724 and Jing 2416 are more sensitive to tribenuron-methyl and imazapic,and suitable as receptor materials for cultivation of anti-herbicide mutant.
引文
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[15] 王健,钟雪梅,吕香玲,等.不同品种玉米对烟嘧磺隆的耐药性研究进展[J].农药学学报,2016,18(3):282-290.
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[18] YOON T Y,CHUNG S M,CHANG S I,et al.Roles of lysine 219 and 255 residues in tobacco acetolactate synthase[J].Biochemical & Biophysical Research Communications,2002,293(1):433-439.
[19] 吴明根,曹凤秋,刘亮.磺酰脲类除草剂对抗、感性雨久花乙酰乳酸合成酶活性的影响[J].植物保护学报,2007,34(5):545-548.
[2] 赵国顺,陈素省,宋丽娜.新型玉米除草剂的防效与评价研究[J].华北农学报,2006,21(S2):203-205.
[3] 降磊,韩文清,尹蓉,等.玉米田除草剂的对比试验[J].山西农业科学,2017,45(7):1146-1148,1152.
[4] 柴超,叶非.安全剂减轻磺酰脲类和咪唑啉酮类除草剂药害的作用机制[J].农药科学与管理,2001,22(6):34-36.
[5] 张金林,石键,李川,等.磺酰脲除草剂对不同作物的敏感度测定[J].河北农业大学学报,1997,20(4):42-46.
[6] 苏旺苍,孙兰兰,吴仁海,等.甲咪唑烟酸模拟残留对后茬作物的敏感性[J].农药,2014,53(4):260-262.
[7] 赵爽,叶非.咪唑啉酮类除草剂的应用及降解[J].植物保护,2009,35(2):15-19.
[8] 柴建方,杜义英,李灵芝,等.一种改进的滤纸卷直立发芽法[J].种子,1997(1):71-72.
[9] 刘伟,王金信,杨广玲,等.不同小麦品种对苯磺隆耐药性差异及其机理[J].植物保护学报,2005,32(3):300-304.
[10] 范志金,陈俊鹏,党宏斌,等.单嘧磺隆对靶标乙酰乳酸合成酶活性的影响[J].现代农药,2003,2(2):15-17.
[11] MALLORY-SMITH C A,RETZINGER E J.Revised classification of herbicides by site of action for weed resistance management strategies[J].Weed Technology,2003,17(3):605-619.
[12] 郑培忠,沈健英.乙酰乳酸合成酶抑制剂的种类及其耐药性研究进展[J].杂草科学,2009(2):4-8.
[13] 陈锡岭,周增莲.磺酰脲类除草剂对玉米敏感性研究[J].河南职技师院学报,2000,28(2):14-17.
[14] 谢娜,毕亚玲,李凌绪,等.不同玉米品种对氯吡嘧磺隆的耐药性差异及其机制[J].植物保护学报,2012,39(6):567-572.
[15] 王健,钟雪梅,吕香玲,等.不同品种玉米对烟嘧磺隆的耐药性研究进展[J].农药学学报,2016,18(3):282-290.
[16] 王焕民.磺酰脲类及咪唑啉酮类除草剂的特性及其应用问题[J].农药科学与管理,1995,16(1):18-21.
[17] KOEPPE M K,BAREFOOT A C,COTTERMAN C D,et al.Basis of selectivity of the herbicide flupyrsulfuron-methyl in wheat[J].Pesticide Biochemistry and Physio-logy,1997,59(2):105-117.
[18] YOON T Y,CHUNG S M,CHANG S I,et al.Roles of lysine 219 and 255 residues in tobacco acetolactate synthase[J].Biochemical & Biophysical Research Communications,2002,293(1):433-439.
[19] 吴明根,曹凤秋,刘亮.磺酰脲类除草剂对抗、感性雨久花乙酰乳酸合成酶活性的影响[J].植物保护学报,2007,34(5):545-548.