高效液相色谱法测定化妆品中的辛酰羟肟酸
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摘要
建立了高效液相色谱法测定化妆品中辛酰羟肟酸含量的分析方法。样品采用甲醇超声提取,经XBridge C_(18)色谱柱分离,高效液相色谱/二极管阵列检测器检测,以保留时间和紫外吸收光谱定性,外标法定量。结果表明,辛酰羟肟酸浓度在1～200 mg/L范围内线性关系良好,相关系数为0.999 9。在20、100、150 mg/L 3个浓度水平进行样品加标实验,回收率为97.2%～104.6%,相对标准偏差在0.48%～1.90%(n=6),方法检出限为0.001%。本方法操作简便、快速、准确,适用于各类化妆品中辛酰羟肟酸含量的测定。
A method for the determination of caprylohydroxamic acid in cosmetics by high performance liquid chromatography was established.Cosmetic samples were extracted by methanol ultrasound,separated by XBridge C_(18) column,detected by diode array detector,and quantified by external standard method.Under the optimized experimental conditions,the linear relationship of caprylohydroxamic acid concentration was good in the range of 1～200 mg/L,and the correlation coefficient was 0.999 9.The recovery was 97.2%～104.6%,the relative standard deviation was 0.48%～1.90%(n=6),and the detection limit was 0.001% when the standard addition experiments were carried out at the concentration levels of 20,100 and 150 mg/L.The method is simple,rapid and accurate.It is suitable for the determination of caprylohydroxamic acid in various cosmetics.
A method for the determination of caprylohydroxamic acid in cosmetics by high performance liquid chromatography was established.Cosmetic samples were extracted by methanol ultrasound,separated by XBridge C_(18) column,detected by diode array detector,and quantified by external standard method.Under the optimized experimental conditions,the linear relationship of caprylohydroxamic acid concentration was good in the range of 1～200 mg/L,and the correlation coefficient was 0.999 9.The recovery was 97.2%～104.6%,the relative standard deviation was 0.48%～1.90%(n=6),and the detection limit was 0.001% when the standard addition experiments were carried out at the concentration levels of 20,100 and 150 mg/L.The method is simple,rapid and accurate.It is suitable for the determination of caprylohydroxamic acid in various cosmetics.
引文
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[2]薛虹宇,印桂琪,Evelyn G.Su.防腐剂的作用及有效性评价方法概述[J].日用化学品科学,2004(7):41-44.
[3]袁仕扬,叶志虹.化妆品用防腐剂浅谈[J].广西轻工业,2009,25(9):29-30.
[4]艾晶,尚衍波,李成必,张行荣.羟肟酸的合成表征及其在浮选中的应用[J].化工矿物与加工,2015,44(8):47-50.
[5]任浏祎,邱航,覃文庆,等.辛基羟肟酸浮选锡石的机理[J].中国矿业大学学报,2017,46(6):1364-1371.
[6]漆静,王少东.羟肟酸类捕收剂的合成及其发展前景[J].云南冶金,2013,42(3):21-25.
[7]唐清,钟宏,王帅,等.羟肟酸类化合物的合成与应用研究进展[J].化工进展,2014,33(03):703-709.
[8]漆静,王少东.羟肟酸类捕收剂的合成及其发展前景[J].云南冶金,2013,42(3):21-25.
[9]李明.化妆品防腐剂及其特点[J].口腔护理用品工业,2018,28(5):35-37.
[10]郭阳,臧埔,郜玉钢,等.化妆品防腐剂的使用现状及进展[J].中南药学,2018,16(9):1258-1263.
[11]范超,张梅,张晶.天然防腐剂的研究进展[J].食品工业,2017,38(10):200-203.
[12]梁辰宇.化妆品防腐剂及其发展[J].科技创新与应用,2015(22):10-11.