黄条金刚竹花期内源激素含量与生理生化指标变化
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摘要
为了探究黄条金刚竹花期内源激素与生理指标含量变化与开花衰老之间的关系,对黄条金刚竹开花与未开花植株各器官花期内源激素(ZR、GA3、ABA及IAA)与生理指标(叶绿素、可溶性蛋白、SOD、POD、MDA、可溶性糖)的含量(活性)进行测定与数据分析。结果表明,开花植株ZR、ABA和IAA总含量高于未开花植株,GA3总含量低于未开花植株;开花植株ZR含量在叶、秆、鞭中下降,花序中先上升后下降。开花植株中,ABA含量在叶、秆和鞭中持续上升。IAA含量在叶、秆、花序中先上升后下降,在鞭中一直下降,GA3含量在各器官中均先上升后下降。开花与未开花植株的各项生理指标变化呈现大致相同的趋势:叶绿素含量均下降,其中开花植株下降幅度大;可溶性蛋白含量降低;SOD和POD活性先上升后下降;MDA含量增加;可溶性糖含量先上升后下降。黄条金刚竹内源激素变化对竹子开花和衰老影响明显,竹子外在衰老表型和衰老过程伴随的生理指标变化相吻合。
In order to explore the relationship between the changes of endogenous hormone, physiological indexes contents and bamboo senility during the flowering period of Sasaella kongosanensis' Aureostriatus', we measured and analyzed the contents of endogenous hormones(ZR, GA3, ABA, IAA) and physiological indexes contents(chlorophyll, soluble protein, SOD, POD, MDA, soluble sugar) in the flowering and non-flowering plants of Sasaella kongosanensis ‘Aureostriatus'. Results showed the total contents of ZR, ABA and IAA in flowering plants were higher than those in non-flowering plants, while the total content of GA3 was lower than that in non-flowering plants. The contents of ZR in flowering plants decreased in leaves, culms and rhizomes, while which increased firstly and then decreased in bamboo inflorescence. ABA content in flowing plants increased in leaves, culms and rhizomes. IAA content firstly increased and then decreased in the leaves, stalks, and inflorescences, and it always decreased in the rhizome. The GA3 content rose firstly and then decreased in all bamboo organs. The contents of physiological indexes in flowering and non-flowering plants showed the same trend, that is, the chlorophyll content decreased, while it decreased greatly in the flowering plants; the soluble protein content decreased; the SOD and POD activities increased firstly and then decreased; the MDA content increased; the soluble sugar content firstly increased and then decreased. The endogenous hormones in Sasaella kongosanensis‘Aureostriatus' have obvious effects on the flowering senescence of bamboo, and the external aging phenotype of bamboo and the physiological indicators accompanying the aging process are consistent.
In order to explore the relationship between the changes of endogenous hormone, physiological indexes contents and bamboo senility during the flowering period of Sasaella kongosanensis' Aureostriatus', we measured and analyzed the contents of endogenous hormones(ZR, GA3, ABA, IAA) and physiological indexes contents(chlorophyll, soluble protein, SOD, POD, MDA, soluble sugar) in the flowering and non-flowering plants of Sasaella kongosanensis ‘Aureostriatus'. Results showed the total contents of ZR, ABA and IAA in flowering plants were higher than those in non-flowering plants, while the total content of GA3 was lower than that in non-flowering plants. The contents of ZR in flowering plants decreased in leaves, culms and rhizomes, while which increased firstly and then decreased in bamboo inflorescence. ABA content in flowing plants increased in leaves, culms and rhizomes. IAA content firstly increased and then decreased in the leaves, stalks, and inflorescences, and it always decreased in the rhizome. The GA3 content rose firstly and then decreased in all bamboo organs. The contents of physiological indexes in flowering and non-flowering plants showed the same trend, that is, the chlorophyll content decreased, while it decreased greatly in the flowering plants; the soluble protein content decreased; the SOD and POD activities increased firstly and then decreased; the MDA content increased; the soluble sugar content firstly increased and then decreased. The endogenous hormones in Sasaella kongosanensis‘Aureostriatus' have obvious effects on the flowering senescence of bamboo, and the external aging phenotype of bamboo and the physiological indicators accompanying the aging process are consistent.
引文
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[20]何见,蒋丽娟,李昌珠,等.光皮树花芽分化过程中内源激素含量变化的研究[J].中国野生植物资源,2009,28(2):41-45.
[21]刘雪华,吴燕.秦岭大熊猫主食竹叶片不同花期内源激素变化及与高光谱的响应关系[J].光谱学与光谱分析,2013,33(9):2512-2518.
[22]刘静.月月竹开花生物学研究[D].南京:南京林业大学,2008.
[23]谢寅峰,林候,张千千,等.鹅毛竹花后叶片衰老生理特性[J].南京林业大学学报(自然科学版),2009,33(6):39-43.
[24]杨淑慎,高俊凤.活性氧、自由基与植物的衰老[J].西北植物学报,2001,21(2):215-220.
[25]谢海平.竹类植物叶片衰老机理研究[D].南京:南京林业大学,2004.
[2]齐飞艳,彭镇华,胡陶,等.毛竹花期不同器官内源激素含量的变化[J].林业科学研究,2013,26(3):332-336.
[3]柴振林.雷竹花期激素和营养动态及其成花机理研究[D].杭州:浙江大学,2005.
[4]丁兴萃.覆盖栽培早竹开花的激素机制研究[J].林业科学,2007,43(7):10-15.
[5]顾琪.淡竹花期激素及营养元素动态变化的研究[D].南京:南京林业大学,2016.
[6]LALNUNMAWIA F,JHA L K,LALENGLIANA F.Preliminary observations on ecological and economical impacts of bamboo flowering in Mizoram(North East India)[J].Journal of Bamboo and Rattan,2005,4(4):317-322.
[7]WANG W,FRANKLIN S B,LU Z J,et al.Delayed flowering in bamboo:evidence from Fargesia qinlingensis in the Qinling mountains of China[J].Front Plant Sci,2016,7:151.
[8]周芳纯.中国观赏竹种简介[J].竹类研究,1993,49(2):68-87.
[9]周芳纯,高尚愚.日本竹林生产和利用情况考察报告[J].竹类研究,1984,3(2):5.
[10]张宪政.植物叶绿素含量测定-丙酮乙醇混合液法[J].辽宁农业科学,1986(3):26-27.
[11]邹琦.植物生理生化实验指导[M].北京:中国农业出版社,1995.
[12]陈建勋,王晓峰.植物生理学实验指导[M].2版.广州:华南理工大学出版社,2002.
[13]刘清华.核桃种子萌发及苗期生长特性研究[D].济南:山东师范大学,2011.
[14]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[15]傅永福,孟繁静.植物的成花生理信号(综述)[J].中国农业大学学报,1998,3(3):1-11.
[16]丁兴萃,田新立,潘雁红,等.早竹覆盖栽培的衰老生理机制[J].林业科学,2009,45(4):41-45.
[17]冯慧,王建红,王茂良.激素在植物开花结果中的作用[J].北京园林,2006(3):28-30.
[18]何奇江,汪奎宏,华锡奇,等.雷竹开花期内源激素、氨基酸和营养成分含量变化[J].林业科学,2005,41(2):169-173.
[19]杨传平,刘桂丰,姜静,等.白桦雄花发育过程中内源激素含量的变化[J].东北林业大学学报,2002,30(4):1-4.
[20]何见,蒋丽娟,李昌珠,等.光皮树花芽分化过程中内源激素含量变化的研究[J].中国野生植物资源,2009,28(2):41-45.
[21]刘雪华,吴燕.秦岭大熊猫主食竹叶片不同花期内源激素变化及与高光谱的响应关系[J].光谱学与光谱分析,2013,33(9):2512-2518.
[22]刘静.月月竹开花生物学研究[D].南京:南京林业大学,2008.
[23]谢寅峰,林候,张千千,等.鹅毛竹花后叶片衰老生理特性[J].南京林业大学学报(自然科学版),2009,33(6):39-43.
[24]杨淑慎,高俊凤.活性氧、自由基与植物的衰老[J].西北植物学报,2001,21(2):215-220.
[25]谢海平.竹类植物叶片衰老机理研究[D].南京:南京林业大学,2004.
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