外源褪黑素对生菜根系生长及内源褪黑素含量的影响
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摘要
以'韩育'奶油生菜为试材,以清水和乙醇溶液为对照,设置了6个不同的褪黑素浓度(0.01、0.10、1.00、10.00、50.00、100.00μmol·L~(-1)),研究了根施不同浓度褪黑素溶液对生菜幼苗种子萌发阶段侧根发育、生菜幼苗根系形态、干鲜质量的影响,进一步筛选出最适外源褪黑素浓度(M1)对生菜叶片与根系内源褪黑素含量的影响,以期为褪黑素对植物生长发育的调控作用提供参考依据。结果表明:与对照相比,1.00μmol·L~(-1)褪黑素处理显著提高了生菜种子萌发阶段侧根数目及生菜幼苗根系的侧根数目和根系的平均直径,降低了根系长度,扩大了根系总表面积。同时,施加褪黑素提高了生菜幼苗的干鲜质量,且该处理下生菜中的褪黑素含量与对照相比提高了9倍。
With 'Hanyu' butter lettuce as the test material and clear water and ethanol as the control,six different melatonin concentrations(0.01 μmol·L~(-1),0.10 μmol·L~(-1),1.00 μmol·L~(-1),10.00 μmol·L~(-1),50.00 μmol·L~(-1) and 100.00 μmol·L~(-1)) were used to study the effects of different concentrations of melatonin on lateral root development of lettuce seedlings at the germination stage,root morphology,fresh and dry weight and endogenous melatonin content of lettuce seedlings were investigated.In order to provide theoretical basis for root development regulation of melatonin in lettuce seedlings.The results showed that 1.00 μmol·L~(-1) significantly increased the number of lateral roots in the germination stage of lettuce seeds,the number of lateral roots,the average diameter of roots and the total surface area of roots of lettuce seedlings,while reduced the length of roots,in comparison with CK and ethanol.Meanwhile,it improved the fresh and dry weight,and endogenous melatonin content of lettuce seedlings was 9 times that of CK.
With 'Hanyu' butter lettuce as the test material and clear water and ethanol as the control,six different melatonin concentrations(0.01 μmol·L~(-1),0.10 μmol·L~(-1),1.00 μmol·L~(-1),10.00 μmol·L~(-1),50.00 μmol·L~(-1) and 100.00 μmol·L~(-1)) were used to study the effects of different concentrations of melatonin on lateral root development of lettuce seedlings at the germination stage,root morphology,fresh and dry weight and endogenous melatonin content of lettuce seedlings were investigated.In order to provide theoretical basis for root development regulation of melatonin in lettuce seedlings.The results showed that 1.00 μmol·L~(-1) significantly increased the number of lateral roots in the germination stage of lettuce seeds,the number of lateral roots,the average diameter of roots and the total surface area of roots of lettuce seedlings,while reduced the length of roots,in comparison with CK and ethanol.Meanwhile,it improved the fresh and dry weight,and endogenous melatonin content of lettuce seedlings was 9 times that of CK.
引文
[1] ARNAO,MARINO B,HERNáNDEZRUIZ,et al.Melatonin:Plant growth regulator and/or biostimulator during stress[J].Trends in Plant Science,2014,19(12):789-797.
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[10] ARNAO M B,HERNáNDEZ-RUIZ J.Protective effect of melatonin against chlorophyll degradation during the senescence of barley leaves[J].Journal of Pineal Research,2008,46(1):58-63.
[11] ARNAO M B.Phytomelatonin:Discovery,content,and role in plants[J].Advances in Botany,2014:e815769.
[12] 曹秀,夏仁学,杨环宇,等.沙培条件下磷、钾、钙亏缺对枳幼苗根系形态及营养吸收的影响[J].植物营养与肥料学报,2014,20(4):981-988.
[13] 徐春梅,谢涛,王丹英,陈松,计成林,章秀福,石庆华.根际氧浓度对水稻分蘖期养分吸收和根系形态的影响[J].中国水稻科学,2015,29(6):619-627.
[14] 范伟国,杨洪强.不同基质对平邑甜茶幼树生长、根系形态与营养吸收的影响[J].植物营养与肥料学报,2009,15(4):936-941.
[15] 程建峰,戴廷波,荆奇,等.不同水稻基因型的根系形态生理特性与高效氮素吸收[J].土壤学报,2007,44(2):266-272.
[16] 陆扣萍,谢寅峰,闵炬,等.不同施氮量对大棚莴苣根系形态及产量和品质的影响[J].土壤,2011,43(4):542-547.
[17] PELAGIO F R,ORTíZ C R,MéNDEZ B A,et al.Serotonin,a tryptophan-derived signal conserved in plants and animals,regulates root system architecture probably acting as a natural auxin inhibitor in Arabidopsis thaliana[J].Plant Cell Physiol,2011:52,490-508.
[18] DAN W,GONG B,SUN S,et al.Promoting roles of melatonin in adventitious root development of Solanum lycopersicumv L.by regulating auxin and nitric oxide signaling[J].Frontiers in Plant Science,2016,7(925):1-11.
[2] 王全喜,张小平.植物学[M].2版.北京:科学出版社,2012.
[3] 李欣欣,赵静,廖红.吲哚乙酸、吲哚丁酸和萘乙酸对大豆幼根生长的影响[J].植物生理学报,2013,49(6):573-578.
[4] 李欣欣,廖红,赵静.GA3、ABA和6-BA对大豆根系生长的影响[J].华南农业大学学报,2014,35(3):35-40.
[5] HERNáNDEZRUIZ J,CANO A,ARNAO M B.Melatonin:A growth-stimulating compound present in lupin tissues[J].Planta,2004,220(1):140-144.
[6] HERNáNDEZ-RUIZ J,CANO A,ARNAO M B.Melatonin acts as a growth-stimulating compound in some monocot species[J].Journal of Pineal Research,2010,39(2):137-142.
[7] CHEN Q,QI W B,REITER R J,et al.Exogenously applied melatonin stimulates root growth and raises endogenous indoleacetic acid in roots of etiolated seedlings of Brassica juncea[J].Journal of Plant Physiology,2009,166(3):324-328.
[8] 杨大进,王竹天,方从容.保健食品中褪黑素含量的测定:GB/T5009.170-2003[S].北京:中国标准出版社:405-408
[9] ARNAO M B,HERNáNDEZ-RUIZ J.Melatonin promotes adventitious-and lateral root regeneration in etiolated hypocotyls of Lupinus albus L.[J].Journal of Pineal Research,2007,42(2):147-152.
[10] ARNAO M B,HERNáNDEZ-RUIZ J.Protective effect of melatonin against chlorophyll degradation during the senescence of barley leaves[J].Journal of Pineal Research,2008,46(1):58-63.
[11] ARNAO M B.Phytomelatonin:Discovery,content,and role in plants[J].Advances in Botany,2014:e815769.
[12] 曹秀,夏仁学,杨环宇,等.沙培条件下磷、钾、钙亏缺对枳幼苗根系形态及营养吸收的影响[J].植物营养与肥料学报,2014,20(4):981-988.
[13] 徐春梅,谢涛,王丹英,陈松,计成林,章秀福,石庆华.根际氧浓度对水稻分蘖期养分吸收和根系形态的影响[J].中国水稻科学,2015,29(6):619-627.
[14] 范伟国,杨洪强.不同基质对平邑甜茶幼树生长、根系形态与营养吸收的影响[J].植物营养与肥料学报,2009,15(4):936-941.
[15] 程建峰,戴廷波,荆奇,等.不同水稻基因型的根系形态生理特性与高效氮素吸收[J].土壤学报,2007,44(2):266-272.
[16] 陆扣萍,谢寅峰,闵炬,等.不同施氮量对大棚莴苣根系形态及产量和品质的影响[J].土壤,2011,43(4):542-547.
[17] PELAGIO F R,ORTíZ C R,MéNDEZ B A,et al.Serotonin,a tryptophan-derived signal conserved in plants and animals,regulates root system architecture probably acting as a natural auxin inhibitor in Arabidopsis thaliana[J].Plant Cell Physiol,2011:52,490-508.
[18] DAN W,GONG B,SUN S,et al.Promoting roles of melatonin in adventitious root development of Solanum lycopersicumv L.by regulating auxin and nitric oxide signaling[J].Frontiers in Plant Science,2016,7(925):1-11.